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Citations for BioLegend :
451 - 500 of 1226 citations for N6 2 aminoethyl 9H purine 2 6 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Membranes were incubated with primary antibodies CD63 (NVG-2; 1:1000; BioLegend), CD81 (Eat-2 ...
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bioRxiv - Biochemistry 2020Quote: ... and rat anti-mouse CD4 F(ab’)2 fragment (clone GK1.5, BioLegend, fragmented according to published procedures45 ...
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bioRxiv - Microbiology 2021Quote: ... and 1:100 BST-2/Tetherin stain (BioLegend Cat# 348415, clone RS38E) in 3% FBS in PBS (Gibco Cat# 14190144 ...
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bioRxiv - Cell Biology 2021Quote: ... was used at 2 μg/ml and soluble anti-mouse CD28 (Biolegend) was used at 8 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... α-IL-2-AlexaFluor700 (1:200; Cat.-No. 503818, Biolegend, San Diego, USA) and α-TNF-α-Pe-Cy7 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 ΔRBM RBD-PE/Cy5.5 (streptavidin-PE/Cy5.5 from BioLegend). The panel for the cohort that received the SARS-CoV-2 spike prime followed by the RBD homotrimer cocktail boost also included SARS-CoV RBD-APC (streptavidin-APC from BioLegend ...
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bioRxiv - Immunology 2021Quote: ... and anti-mouse-IL-2-PE-Cy7 (Biolegend Cat# 503832, RRID: AB_2561750). Stained cells were acquired by FACSAriaIII Flow Cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... and AlexaFluor-647-conjuaged anti-CD18 antibody (M18/2, Biolegend, 1:100). Cells were washed with FACS buffer ...
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bioRxiv - Pathology 2021Quote: ... pan aSyn (N-terminal directed, SYN303 [aa 2-12 Cat# 82401, Biolegend] ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 μg/ml soluble anti-mouse CD28 antibody (102116, BioLegend, USA) in RPMI 1640 medium (21870-076 ...
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bioRxiv - Immunology 2021Quote: ... PE-anti-human-Nectin-2 (TX31) (all from BioLegend, San Diego, CA). The following antibodies were used for in vivo blocking experiments ...
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bioRxiv - Immunology 2020Quote: ... KLRG1 APC-Cy7 (clone 2FI/KLRG1, BioLegend, cat# 138426, 2:100 dilution); CD279 (PD-1 ...
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bioRxiv - Immunology 2020Quote: ... CD279 (PD-1) BV650 (clone EH12.2H7, BioLegend, cat# 329950, dilution 2:100); CD45RA BV510 (clone HI100 ...
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bioRxiv - Neuroscience 2023Quote: ... or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend, 148603) for 30 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Media was additionally supplemented with 10 ng/mL murine IL-2 (BioLegend) prior to resuspension and subsequent passaging ...
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bioRxiv - Immunology 2024Quote: ... 1-2×106 cells were incubated with Fc receptor blocking reagent (BioLegend) for 5 min at RT ...
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bioRxiv - Immunology 2024Quote: ... and with 100 U/ml IL-2 (BioLegend, San Diego, CA, USA) (referred to as “Bead activated T cells” or “nonspecific activation” ...
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bioRxiv - Immunology 2024Quote: ... the gels were incubated with 2 μg/ml Calcein-AM (BioLegend, 425201) and 1 μg/ml Propidium Iodide (MP Biomedicals ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with 2× Cyto-FastTM Perm Wash solution (CFPWS; BioLegend) and incubated with PGP9.5 for 20 min at RT ...
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bioRxiv - Immunology 2022Quote: ... 1-2×106 cells were incubated with Human TruStain FcX (BioLegend #422302) and Fixable Viability Stain 510 (BD #564406 ...
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bioRxiv - Neuroscience 2022Quote: ... Fisher) along with 2 µl of live/dead cell stain (DRAQ7, BioLegend) per ml and then FACS sorted.
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bioRxiv - Immunology 2022Quote: ... Rat anti-CD138 antibody (rat anti-CD138, clone 281-2, Biolegend, 142502) was applied to each section and specific bounds were visualized using a Alexa Fluor488-conjugated anti-rat antibody (JacksonImmunoResearch ...
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bioRxiv - Immunology 2023Quote: ... anti-CD138 Alexa Flour 647 (1:80 dilution, clone 281-2, BioLegend), donkey anti-mouse IgG Alexa Fluor Plus 405 (1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were collected and analyzed for IL-2 by ELISA (BioLegend).
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bioRxiv - Bioengineering 2024Quote: ... and cells were cultured with 0.5 µg/mL recombinant IL-2 (BioLegend) in RPMI + 10% heat-inactivated FBS for 24 h before use in NK cell cytotoxicity or flow cytometry experiments.
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bioRxiv - Immunology 2024Quote: ... cells were stained with 2 µl of TotalSeqTM-C hashing antibodies (Biolegend) in wash buffer for 30 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... T cells were supplemented with 2 µg/mL anti-CD28 (37.51; Biolegend), seeded in a F-bottom 96-well plate precoated with 3 µg/mL anti-CD3 (145-2C11 ...
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bioRxiv - Immunology 2024Quote: ... permeabilized and stained with anti-IL-2 antibody (Clone, JES6-5H4, Biolegend) and analyzed using a BD LSR Fortessa (BD Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-pERK1/2 (Thr202/Tyr204) antibody (6B8B69) was purchased from BioLegend. Dead cells were stained with the LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit for 633- or 635-nm excitation (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... 2 hours after start of restimulation 5μg/mL of Brefeldin A (BioLegend) was added to each well for the remaining 3 hours ...
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bioRxiv - Bioengineering 2024Quote: ... antibody while staining with B2M (Beta-2 Microglobulin) (1:200, 316312 BioLegend) antibody for 60 min in the dark at +4⁰C to identify pluripotency state and B2M KO of the cells before acquiring on flow cytometer.
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bioRxiv - Bioengineering 2020Quote: ... IL-6 (Biolegend) and TGF-β (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... IL-6 (Biolegend) or Tocilizumab (a kind gift from Dr Ceri Roberts in Professor Leonie Taams lab ...
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bioRxiv - Immunology 2023Quote: ... IL-6 (BioLegend® Cat ...
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bioRxiv - Microbiology 2022Quote: ... IL-6 (Biolegend), TNF-α (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... anti-IgD-FITC (1:20 dilution, clone IA6-2, cat. no. 348206, BioLegend), anti-IgM-FITC (1:20 dilution ...
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bioRxiv - Microbiology 2021Quote: ... in the presence of 2 μg/mL soluble αCD28 antibody(clone CD28.2, Biolegend). RNAi knockdown of DCAF1 was performed as described before52 using ON-TARGET plusHuman DCAF1 siRNA - SMARTpool (Dharmacon ...
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bioRxiv - Bioengineering 2022Quote: ... and 50 μg/mL anti-IL-2 Alexa Fluor 488 (Biolegend, Catalog: 500314) in 50μL washing buffer per 6 μL nanovial volume at 37°C for 30 minutes protected from light ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were collected to measure IL-2 by ELISA (ELISA MAX Deluxe, BioLegend) and proliferation assessed by methyl-3[H]-thymidine (1 µCi/well ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... and a SARS-CoV-2 nucleocapsid antibody (clone A20087H, mouse IgG2b isotype, BioLegend) followed by a secondary antibody stain with PE-Cy7-conjugated anti-mouse IgG2b (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... CD46 APC (targets MCP protein) clone TLA-2-10 (cat# 352405, BioLegend®), CD49d APC (targets α4 integrins ...
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bioRxiv - Microbiology 2021Quote: ... rat anti-mouse CD138-BV605 (clone 281-2, 1:200, Biolegend, cat#142516), rat anti-mouse CD19-BV785 (clone 1D3 ...
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bioRxiv - Immunology 2020Quote: ... 2 μg/ml rat anti-mouse CD40 antibody (BioLegend, San Diego, California, USA), 2 ng/ml interleukin 7 (IL7 ...
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bioRxiv - Immunology 2021Quote: ... and seeded into 2 μg/mL anti-CD3 antibody (clone: 145-2C11, BioLegend) pre-coated 24-well plates ...
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bioRxiv - Immunology 2023Quote: ... followed by testing with a murine IL-2 commercial sandwich ELISA kit (BioLegend).
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bioRxiv - Immunology 2023Quote: ... followed by testing with a murine IL-2 commercial sandwich ELISA kit (BioLegend).
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bioRxiv - Immunology 2023Quote: ... followed by testing with a murine IL-2 commercial sandwich ELISA kit (BioLegend).
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bioRxiv - Immunology 2023Quote: ... followed by testing with a murine IL-2 commercial sandwich ELISA kit (BioLegend).
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bioRxiv - Microbiology 2023Quote: ... 10 µM each) and 50 U/mL of recombinant mouse IL-2 (BioLegend) overnight ...
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bioRxiv - Immunology 2023Quote: ... 2/3 of the cells were fixed with 750μL fixation buffer (BioLegend, #420801) for 30min ...