Labshake search
Citations for BioLegend :
701 - 750 of 1226 citations for N6 2 aminoethyl 9H purine 2 6 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... HEPES (10 mM) and L-glutamine (2 mM) supplemented with 25 ng/mL M-CSF (Biolegend, 574806) for 6 days.
-
bioRxiv - Molecular Biology 2024Quote: ... Red blood cells were lysed using a 2-minute incubation with RBC lysis buffer (BioLegend, catalog #420302) and the cells were then washed ...
-
bioRxiv - Immunology 2024Quote: ... eleven different TotalSeq™-C anti-human Hashtag oligo-antibodies (0.5 g/106cells; BioLegend, Supplementary table 2) were added to the PBMCs stimulated with myelin peptides to enable further multiplexing of the sorted samples at the single-cell TCR sequencing step ...
-
bioRxiv - Bioengineering 2024Quote: ... they were incubated overnight at 4 °C with mouse anti-βIII tubulin (2 µg/mL, TUBB3, Biolegend). After the primary antibody incubation ...
-
bioRxiv - Genetics 2024Quote: ... they were stained with 200 ng/1 million cells PE CD46 (TRA-2-10) flow antibody (BioLegend) and the CD46 negative population was separated by fluorescence-activated cell sorting ...
-
bioRxiv - Cell Biology 2020Quote: ... Supernatant was discarded and the pellet was resuspended in 100µl FACS Stain Buffer (2% FBS in PBS) with 1µl anti-CD45-APC/Cy7 (clone 30-F11, BioLegend) and 1µl anti-EPCAM-AlexaFluor488 (clone G8.8 ...
-
bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Digested meniscus cells were re-suspended in flow buffer (2% FBS/PBS) and stained with Sca1 (BioLegend, 108131), Cd90 (BioLegend ...
-
bioRxiv - Neuroscience 2021Quote: ... Brilliant Violet 421 or Alexa Fluor 594 conjugated anti-mouse CD8a (2 μg/mL; clone 53-6.7; BioLegend) was used ...
-
bioRxiv - Molecular Biology 2021Quote: ... and about 2×105 cells were resuspended in 100μl binding buffer (FITC Annexin V Apoptosis Detection Kit, Biolegend). Subsequently ...
-
bioRxiv - Molecular Biology 2021Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: ... Cell hashing antibodies targeting human Beta-2-microglobulin (clone 2M2) and CD298 (clone REA217) were purchased from Biolegend and Miltenyi Biosciences ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-Vimentin (Santa-Cruz; 2 μg/mL) and mouse anti-placental alkaline phosphatase (Biolegend; 1 μg/mL). Immunostaining for hCMV was performed as previously described [32] ...
-
bioRxiv - Immunology 2022Quote: ... with PE-conjugated anti-mouse H-2 or I-A/I-E Ab and corresponding isotype controls (BioLegend) (for the MHC study) ...
-
bioRxiv - Immunology 2022Quote: Whole blood inoculated with SARS-CoV-2 was blocked with 5μl of TruStain (Biolegend, San Diego, California, # 422302) for 10 minutes at room temperature and then stained for granulocyte and monocyte markers ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
-
bioRxiv - Neuroscience 2022Quote: ... Dead cells were excluded by labelling with the cell viability dye Zombie Aqua (2 ng/ml, BioLegend, 423101) that was added to the single cell suspension before sorting ...
-
bioRxiv - Molecular Biology 2020Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2022Quote: ... Both cell fractions were stained with cell hashing antibodies (1:1000, #2 for heart, #3 for blood, Biolegend) and Propidium iodide (1:100 ...
-
bioRxiv - Bioengineering 2023Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% BSA in PBS) and stained with 7-AAD (BioLegend) as a dye for cell viability assay ...
-
bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...
-
bioRxiv - Immunology 2024Quote: ... Mouse and human T cells were washed three times with RPMI 10% FBS and 2.5 x 105 cells were added to each well with 2 µg/ml soluble anti-CD28 (37.51 or CD28.2, Biolegend). For co-culture experiments ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 2% PFA for 20min and permeabilized with Perm/Wash buffer (Biolegend, Cat No.421002) for 30mins ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2 X10^5 cells were then resuspended to be stained with 5ul of Annexin V-FITC (#640945, Biolegend) in 100ul of Annexin V binding buffer (10mM HEPES ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
-
bioRxiv - Bioengineering 2022Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% FBS in PBS) and stained with 7-AAD (BioLegend), and the indicated cell-surface marker ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2% FCS and 0.09% NaN3) and stained with fluorophore-conjugated antibodies (CD146-APC, SHM-57 or P1H12, BioLegend), (EPCAM-FITC ...
-
bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 24h and extracellular IL-2 was measured using the Biolegend ELISAMax Deluxe Kit (Biolegend), according to manufacturer instructions.
-
bioRxiv - Immunology 2024Quote: ... 0.25 x 106 cells were stimulated with either plate-bound 11-CD3 (BioLegend; RRID: AB_11149115; 2 µg/mL) and plate-bound 11-CD28 (BioLegend ...
-
bioRxiv - Genomics 2024Quote: ... and resuspended in 50ul PBS plus 2% FBS containing 1:100 diluted anti-CD69-APC antibody (BioLegend 310909). Cells were stained in the dark on ice for 30 min ...
-
bioRxiv - Immunology 2024Quote: ... The culture medium was supplemented with 10 ng/mL IL-2 and 25 ng/mL IL-15 (Biolegend). On the next day ...
-
bioRxiv - Immunology 2024Quote: ... The pooled sample was then stained with diluted TotalSeq-C cell surface marker antibodies (BioLegend, Supplementary Table 2) in PBS containing 2% FBS for 30 minutes at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... Cells were washed 3 times with PBS containing 2% FBS before 7-AAD viability staining solution (BioLegend, #420404) was added and cells were subjected to FACS (BD FACSAria 4-laser digital flow cytometer with FACSDiva v8 software) ...
-
bioRxiv - Immunology 2024Quote: ... Red blood cells were then removed with a 2-minute incubation in RBC Lysis Buffer (10X) (BioLegend 420301). The solution was centrifuged again at 300 x g ...
-
bioRxiv - Cell Biology 2021Quote: ... Thrombin Receptor Activator Peptide-6 (TRAP-6; 30μM) or Adenosine Diphosphate (ADP; 30μM) along with CD42b-BV421 (1:60, Biolegend), PAC-1 FITC (1:12 ...
-
bioRxiv - Immunology 2022Quote: ... rIL-6 (20 ng/ml; Biolegend, #575706), rIL-23 (20 ng/ml ...
-
bioRxiv - Immunology 2021Quote: ... Bcl-6 FITC (Biolegend 358513, 1:100), and Glut-1 AlexaFluor 647 (Abcam ab115730 ...
-
bioRxiv - Immunology 2020Quote: ... rIL-6 (20 ng/ml; Biolegend, #575706), rIL-23 (20 ng/ml ...
-
bioRxiv - Immunology 2023Quote: ... anti-IL-6 (clone MP5-20F3, BioLegend), and isotype control (#RTK2071 ...
-
bioRxiv - Immunology 2023Quote: ... IL-6 (R&D) and IL12B (Biolegend) ELISAs were preformed to manufacturers instructions
-
bioRxiv - Microbiology 2024Quote: ... HLA-DR (G46-6; BioLegend, Cat# 562304), CD80 (2D10 ...
-
bioRxiv - Immunology 2022Quote: ... anti-IL-6-FITC (Biolegend, Cat# 501103), anti-IFN-γ-Alexa Fluor 700 (Biolegend ...
-
bioRxiv - Pathology 2022Quote: Serum levels of IL-6 and TNFα were measured by a mouse ELISA MAX Standard IL-6 kit (BioLegend) and a mouse ELISA MAX Standard TNFα kit (BioLegend).
-
bioRxiv - Immunology 2021Quote: ... Extracellular staining was performed in FACS buffer (PBS + 2% FBS + 2mM EDTA) with the following antibodies: CD4 (GK1.5, Biolegend), CD8 (53-6.7 ...
-
bioRxiv - Immunology 2022Quote: Serum samples were screened for neutralization using LEGENDplex SARS-CoV-2 neutralizing antibody assay (BioLegend, San Diego, CA, USA) following manufacturer recommendations ...
-
bioRxiv - Immunology 2021Quote: ... 2.1 mmol/L ultra-glutamine in the presence of Brefeldin A (5 μg/ml) and monensin (2 μM; BioLegend). After 5 hours of incubation ...
-
bioRxiv - Immunology 2021Quote: ... and stained with fluorochrome-labeled anti-mouse IFN-γ/TNF-α/IL-2/IL-4/IL-5 mAbs (Biolegend). LSRFortessa was used to acquire the flow cytometry data ...
-
bioRxiv - Cancer Biology 2020Quote: ... we collected the beads and labeled them with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... then resuspended in FACS buffer containing 2 ug/ml APC/Cy7 anti-human HLA-A,B,C (BioLegend, #311425). Cells were incubated with the antibody on ice for 20 minutes in the dark ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were blocked in FSC buffer (2% FBS/1x PBS) containing 0.5 µg anti-CD16/32 antibody (TruStain FcX Plus, BioLegend) for 10 min at 4°C to block non-specific binding of antibodies via Fc receptors ...