Labshake search
Citations for Roche :
401 - 450 of 1814 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 5 μg of plasmid and dsRNA were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) every 3 days four times ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were washed 24 hours after transfection with phosphate-buffered saline and lysed with 100 μl of Reporter Lysis Buffer (Roche), and lysates were assayed for luciferase activity using Bright-Glo™ (Promega ...
-
bioRxiv - Pathology 2023Quote: ... in HEK 293T cells (3×105 cells per well in 6-well plates) using X-treme gene transfection reagent (Roche). Pseudotyping was achieved by co-transfecting pHEF-VSVg (400 ng/well) ...
-
bioRxiv - Genomics 2024Quote: ... Cells were then transfected with the Tet-pLKO-puro plasmids targeting either UHRF1 or DNMT1 transcripts and the accompanying lentiviral packaging (psPAX2) and envelope (pMD.2G) plasmids with Opti-MEM and Xtreme HP Gene transfection reagent (Roche) per the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... and subsequently transfected without (Mock) or with ultrapure LPS (10 μg/ml) into the cytosol with DOTAP Liposomal Transfection Reagent (Roche) for 4 hours.
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) and cells were fixed 5–6 days later ...
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
-
bioRxiv - Cancer Biology 2024Quote: ... human 293FT cells were grown on 10cm dishes and transfected with sgRNA-containing vector (pX330A-1x2-cNAT10/PITCh) and donor vector using Xtremegene 9 transfection reagent (Roche). 72 h after transfection ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected with 1 µg of shRNA/cDNA and 1 µg of viral packaging plasmids (250 ng pMD2.G and 750 ng psPAX2) using 6 µl of Xtreme Gene 9 transfection reagent (Roche). After 24 hours of transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... Transfection of 106 cells in a 6-well plate was performed using 5 μl of transfection agent (X-tremeGENE HP Reagent, Roche), 10 μL of bacmid and 85 μL of SF900II-SFM medium (Gibco) ...
-
bioRxiv - Immunology 2024Quote: The retroviral recombination substrate pINV-12/23 was introduced into primary leukemic cells utilizing X-treme GENE HP DNA Transfection Reagent (Roche). Recombina-tion efficiency of pINV-12/23 was evaluated through flow cytometry analysis for mouse CD90 (mCD90 ...
-
bioRxiv - Cell Biology 2024Quote: ... Lentiviral vectors expressing sgRNAs were transfected into HEK293T cells with lentiviral packaging vectors CMV VSV-G and psPAX2 using XtremeGene 9 transfection reagent (Roche). After 24 h ...
-
bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-transfected with sgRNA expression vectors and lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 2:1:1 ratio using X-tremeGENE 9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... expression vectors were co-transfected into HEK293T cells with the lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 1:1:1 ratio using X-tremeGENE9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... the Roche LightCycler® 480 System (Cat# 05015243001) in combination with 384-well plates (FrameStar, Cat# 4ti-0381/DBC, and Roche, Cat# 04729757001) was used ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Triplicate no-template controls contained 1x KAPA Sybr Fast Low-Rox qPCR Master Mix (KK4621; Roche Sequencing, Pleasanton, CA, USA) with 200 nM each primer and water only ...
-
bioRxiv - Immunology 2021Quote: ... Control siRNA (qiagen) and CXCR4 siRNA (SMARTPool, Dharmarcon) were diluted in DOTAP (1,2-dioleoyl-3-trimethylammonium-propane; Roche Applied Sciences). The mix was gently mixed and incubated at room temperature for 15 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... for genes of interest and Gapdh (a normalization control for amplification efficiency) on a LightCycler 480 Real-Time PCR machine (Roche). The primer sequence used for qPCR analysis is listed in Supplementary Table 12 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The reaction for the internal control was performed using dual detection of FAM and YAK/VIC in a Lightcycler 480 (Roche), the detection of the N-gene was done in a QuantStudio5 cycler (Thermo Fisher).
-
bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed with sigA/Rv2703 gene as control and fold change was measured by ΔΔCt method using Fast Start universal master mix (Roche). The following primers were used ...
-
bioRxiv - Cell Biology 2022Quote: All controls contained 4% (v/v) 25x concentrated protease inhibitor cocktail solution (cOmplete Protease inhibitor cocktail tablets, Roche, Mannheim, Germany) in accordance with the PPP samples.
-
bioRxiv - Cancer Biology 2022Quote: ... genomic (g)DNA was extracted both from primary Irf4−/− tumours as well as FACS-sorted control B220+mIgM- BM fr.A-D cells using the High Pure PCR Template Preparation kit from Roche (11796828001). Integrity of resultant gDNA was confirmed in a 2 % Agarose gel ...
-
bioRxiv - Immunology 2022Quote: ... PBMCs were incubated with an equimolar amount of DMSO as negative control and with phytohemagglutinin (5 μg/ml) (PHA, Roche) as a positive control ...
-
bioRxiv - Developmental Biology 2019Quote: Total DNA was isolated from L3 larvae or 5-days old adults of Iduna homozygous mutants and control sequencing strain using the Roche genomic DNA extraction kit (Roche). To confirm the mutant line ...
-
bioRxiv - Microbiology 2020Quote: ... thus one negative control is placed after every 5 samples on a Light cycler 480 multiwell plates 96-well plate (Roche).
-
bioRxiv - Synthetic Biology 2020Quote: ... Library quality control and quantification were performed using the KAPA Library Quantification Kit for Illumina platforms (Kapa Biosystems, Massachusetts, USA) following the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... The PCR was performed with single sense (negative control) or antisense primers in presence of DIG-labelled deoxynucleoside triphosphates (dNTPs) (Roche). Freshly hatched J2s were fixed and hybridized with the probes following the protocol of de Boer et al ...
-
bioRxiv - Immunology 2020Quote: ... Cyclophilin B (control) and PPARγ siRNA (SMARTPool, Dharmarcon) were diluted in DOTAP (1,2-dioleoyl-3-trimethylammonium-propane; Roche Applied Sciences). The mix was gently mixed and incubated at room temperature for 15 min ...
-
bioRxiv - Genomics 2020Quote: ... Quantification and quality control of the final HiChIP library was conducted using a Bioanalyzer and KAPA quantification kit (Kapa Biosystems). Libraries underwent Next Generation Sequencing on a HiSeq 2500 generating 100 bp paired-ends.
-
bioRxiv - Cancer Biology 2022Quote: Cells were transfected with YAP/TAZ luciferase reporter construct along with pRL-CMV-Renilla luciferase control reporter vector by Fugene6 (Roche). Luciferase activity was measured with Dual Luciferase Reporter Assay System (Promega ...
-
bioRxiv - Microbiology 2023Quote: Cells transiently expressing M2-1-GFP or GFP as control were lysed with cell lysis buffer (CST) supplemented with complete mini-EDTA-free protease inhibitors (Roche) on ice and cell debris removed by centrifugation ...
-
bioRxiv - Neuroscience 2022Quote: ... we included snRNA-seq data on temporal and prefrontal cortex control samples from a further unrelated 87 unique subjects from Roche. Details of the Roche control samples and nuclei isolation are as previously described [12] ...
-
bioRxiv - Genomics 2023Quote: ... The batches of isolated RNA were arranged sequentially in 96-well plates for quality control and preparation of libraries using the standard automated KAPA stranded mRNA library preparation protocol (Roche). One sample was lost in transport before library preparation (HG03478 (MSL)) ...
-
bioRxiv - Molecular Biology 2020Quote: ... protease (Cat. no. 03115836001, Roche) digested ...
-
bioRxiv - Cell Biology 2021Quote: ... and KpnI (Roche, cat#10899186001) and ligation with T4 DNA ligase (Applichem ...
-
bioRxiv - Cell Biology 2022Quote: ... GFP antibody (Cat#11814460001 Roche), IKKε Rabbit Ab (#2905S ...
-
bioRxiv - Molecular Biology 2022Quote: ... G418 (Roche, Cat no. 04727894001) and incubated at 37 °C in 5 % CO2.
-
bioRxiv - Biochemistry 2022Quote: ... dNTPs (cat. no.11969064001; Roche), 0.5mM ...
-
bioRxiv - Biochemistry 2022Quote: Pronase (Roche Cat No.: 10165921001) was dissolved in de-ionized water at a stock concentration of 10 mg/ml ...
-
bioRxiv - Cell Biology 2022Quote: ... and phosphatase (Roche, cat# 04906845001) inhibitors and lysed for 30 min at 4°C with rotation ...
-
bioRxiv - Neuroscience 2022Quote: ... containing complete (cat: 04693116001, Roche) protease inhibitor ...
-
bioRxiv - Cancer Biology 2022Quote: ... and PhosSTOP (Roche, Cat# 4906837001). Protein samples of cleared lysates were measured using BCA assay (Thermo Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... or DH (Roche; cat: 5401089001) and 70 U/ml hyaluronidase (Merck ...
-
bioRxiv - Cancer Biology 2020Quote: ... protease inhibitor (Roche, Cat# 04693124001), and propidium iodide (ACROS ...
-
bioRxiv - Microbiology 2022Quote: ... and phosSTOP (Roche, Cat. 4906845001). Lung AT2 lysates were made using Laemmli sample buffer (Bio-Rad ...
-
bioRxiv - Plant Biology 2022Quote: ... or GFP (Cat# 1181446001, Roche) were incubated for 1 h at RT in 3% (wt/vol ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNAse-free (Roche, Cat# 4716728001) at 37°C for 1 hr and purified by phenol-chloroform extraction as described before ...
-
bioRxiv - Molecular Biology 2022Quote: ... EDTA free (Roche Cat. 4693132001)] ...
-
bioRxiv - Developmental Biology 2022Quote: ... protease inhibitor (Roche Cat # 05892970001) and phosphatase inhibitor (Roche Cat # 04906837001) ...