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Citations for Roche :
551 - 600 of 1814 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Colcemid (1:100, Cat.# 10295892001, Roche) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... POD (Roche, Mississauga, ON, Cat. 11684817910) was used as per manufacturer’s protocol to detect apoptosis in FFPE mouse brain tissue sections.
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bioRxiv - Cancer Biology 2024Quote: ... HA (Roche Applied Science, cat. 11867423001). Phospho-Ser/Thr antibody (cat ...
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bioRxiv - Cell Biology 2023Quote: ... The anti-GFP (Roche, Cat#11814460001) antibody was used for detection.
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bioRxiv - Cancer Biology 2021Quote: ... Total cell lysates from COS-1 cells 24 hr post transfection were prepared using 300 μl of KAc interaction buffer (Roche Applied Science). GST fusion proteins were bound to glutathione-Sepharose beads (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Transfection with full-length nV5-WT or nV5-P112L (0.8 μg DNA/well) was performed 24 h post-seeding using X-tremeGENE™ 9 DNA Transfection Reagent (Roche, Basel, Swityerland). 24 h post-transfection ...
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bioRxiv - Cell Biology 2021Quote: Two days after transfection, cells were lysed with RIPA Lysis Buffer (P0013C, Beyotime) supplemented with 1x cOmpleteTM EDTA-free Protease Inhibitor Cocktail (Roche, Basel, Switzerland) for 40 min at 4 °C and then clarified by centrifugation at 12,000 × g for 20 min ...
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bioRxiv - Microbiology 2020Quote: ... plasmids were transfected into HeLa cells growing on 35 mm glass bottom dishes using X-treme GENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols.
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bioRxiv - Microbiology 2019Quote: ... the ORF50 trans-complementing BHK-21 cells were transfected with 2 μg of ΔORF50 BAC DNA using FuGENE HD Transfection Reagent (Roche, Mannheim, Germany). When cells showed a visible CPE ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were treated with the oligonucleotide mixed with DOTAP liposomal transfection agent (35 μg/ml) (#11811177001, Roche Diagnostics GmbH, Mannheim, Germany) for 3 to 6 hrs (16) ...
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bioRxiv - Microbiology 2021Quote: Plasmids were transfected into HeLa cells growing on 100 mm dishes using X-treme GENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols and processed for TEM at 18 hours post transfection ...
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bioRxiv - Cell Biology 2023Quote: ... UGGT2-/- and UGGT1/2-/-cells were transfected with 1 µg Trop-2-pEYFP-N1 or Trop-2-Q118E-pEYFP-N1 plasmids using X-tremeGENE 9 DNA transfection reagent (Roche, Basel, Switzerland) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: Cells were plated to ∼50% confluence in 6-well plates and 30pmol of siRNA against CTNNB1 (siCTNNB1) or non-targeting siRNA (siNT) (Dharmacon) were transfected using XtremeGENE™ siRNA Transfection Reagent (Roche, Merck) as per manufacturer’s instructions (for SW620 150pmol siRNA was used) ...
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bioRxiv - Immunology 2024Quote: 293T cells were co-transfected with plasmids encoding cognate heavy and light chains using the Xtreme Gene 9 DNA transfection reagent (Roche, Basel, Switzerland), then grown in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2024Quote: ... The pIEx-4-BmU6-Cad96ca-gRNA-Cas9-GFP-P2A-Puro or pIEx-4-BmU6-Fgfr1-gRNA-Cas9-GFP-P2A-Puro recombinant vectors were transfected into HaEpi cells with transfection reagent (Roche, Basel, Switzerland). After 48 h of vector transfection (cells can be observed to express green fluorescent protein) ...
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bioRxiv - Cancer Biology 2021Quote: ... control and Capecitabine treated tumors were digested for 2 h at 37 °C with a cocktail of Collagenase I (Roche, Ref: 11088793001) and Hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: Cell-free histone levels were assessed in plasma from healthy controls and COVID-19 patients using a photometric enzyme immunoassay (Cell Death Detection ELISAPLUS kit, Roche Applied Science) that measures histone-associated DNA fragments according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... an equimolar amount of DMSO was used to stimulate the cells in triplicate wells and as positive controls phytohemagglutinin (PHA, Roche, 1μg/ml) and a cytomegalovirus MP (CMV ...
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bioRxiv - Microbiology 2022Quote: ... Cell equivalents were calculated according to amplification of the β-globin gene (two copies per diploid cell) with commercially available materials (Control Kit DNA; Roche Diagnostics). 2-LTR circles ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from the mPFC tissues of CamK2A-Cre;Gpr158fl/fl mice and the controls (n = 4) at the age of 8 weeks with Tripure Isolation Reagent (Roche, Mannheim, Germany). RNA-sequencing (RNA-seq ...
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bioRxiv - Developmental Biology 2023Quote: ... Average library size and quality control were performed using a Fragment Analyzer (High Sensitivity NGS kit) and qPCR (Roche Light Cycler 480). CUT&RUN libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... brain slices were permeabilized by 0.3% triton in PBS and positive control slices were incubated with Dnase I recombinant (Roche, Basel, Switzerland, #4536282001) (1,000 units) ...
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bioRxiv - Pathology 2020Quote: Cells were harvested in cell lysis buffer (Cat.#P0013, Beyotime) containing protease inhibitor cocktail (Cat.#04693159001, Roche) and homogenized by ultrasonication ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.4 μM Benzamidine (Sigma, Cat# B6506 and cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche, Cat# 11873580001). The suspension was flash frozen in droplets ...
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bioRxiv - Cell Biology 2023Quote: ... Lysis buffer included 142.8ul 7x protease/phosphatase inhibitor stock (mixture of Roche Cat#11836153001 and Cat#4906845001), 100ul 10x RIPA buffer ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... We induced transient expression of the plasmids in 106 cells resuspended in 2 ml using the polymer X-tremeGENE 9 DNA Transfection Reagent (Roche Applied Sciences 06365787001) following manufacturer’s instructions (1 μg total DNA and 3 μl reagent in 100 μl FBS-free culture medium) ...
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bioRxiv - Neuroscience 2022Quote: N2a cells were collected for expression validation from the 12-well plate at 24 h post transfection in RIPA buffer (Cell Signalling Technologies; Catalog No. 9806) in the presence of phosphatase inhibitor (Roche; Catalog No. 04906837001) and protease inhibitor (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of beta-globin was performed by a commercially available human genomic DNA kit (The LightCycler Control Kit DNA, Roche Diagnostics, Basel, Switzerland)69.
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bioRxiv - Developmental Biology 2023Quote: The threshold cycle of each target and reference gene amplification in control and mutant embryos was determined automatically by LightCycler® 96 Software (Roche Diagnostics, USA). Fold change in mutants versus controls was calculated with delta-delta-C(t ...
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bioRxiv - Cell Biology 2022Quote: ... as the internal reference plasmid were co-transfected with the P65 and P50 expression vector pAd5 E1-CMV-P65/P50 (each 300 ng) or the control vector pAd5 E1-CMV-MCS (600 ng) into the cells using X-tremeGENE HP Reagent (Roche, Indianapolis, IN, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: GDF15 measurements in both the HG vs control study and in Generation Scotland were measured on a Cobas e411 analyser (Roche Diagnostics, Basel, Switzerland) using the manufacturer’s reagents and quality control material ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets were lysed with CST lysis buffer (Cat #9803) that was supplemented with phosphatase (Roche, Cat #04906845001) and protease inhibitors (Roche ...
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bioRxiv - Cancer Biology 2023Quote: Total proteins were extracted in RIPA buffer (Cat. #89901, Pierce) containing complete protease inhibitor cocktails (Cat. #04693116001, Roche) and phosSTOP (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Exonic regions were captured using IDT xGen Exome Research Panel v1.0 (Cat # 1056114) with xGen Universal Blockers (Cat # 1075474) and Roche KAPA HyperCap workflow 3.0 (Roche) according to manufacturer protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 protease inhibitor tablet (Roche, Cat. 11873580001)) using a dounce homogenizer ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-HA (Roche Cat# 11867423001, RRID:AB_390918), mouse anti-Pan-Neurofascin external (clone A12/18 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µl proteinase K (Roche, Cat 10910000) was then added and the mix was incubated at 55℃ for 90 min to elute DNA from beads.
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bioRxiv - Developmental Biology 2021Quote: ... and Phosphatase inhibitor (Roche Cat. No. 04906845001), according to the manufacturer instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were washed in MABT before stained with NBT/BCIP (Roche, Cat#11681451001). Stained embryos were cleared in BBA solution (2:1 Benzyl benzoate ...
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bioRxiv - Molecular Biology 2021Quote: ... and Transcriptor Reverse Transcriptase (Roche, Cat# 03531287001) following manufacturer’s instruction.
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bioRxiv - Molecular Biology 2021Quote: ... protease inhibitor cOmplete ULTRA (cat# 05892791001, Roche) and phosphatase inhibitor PhosSTOP (cat# 04906837001 ...
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bioRxiv - Biophysics 2019Quote: ... 4 mg/ ml Catalase (Roche, Cat#10106810001) and 1mM Trolox ((±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid ...
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bioRxiv - Cell Biology 2019Quote: ... 25 μg/ml insulin (Cat.#1376497, Roche), 50 μg/ml 7.5% BSA and 2 mM L-glutamine (Cat.#25030-024) ...
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bioRxiv - Cell Biology 2019Quote: ... KAPA single-indexed adapters (Cat#08005699001, Roche) were added to the A-tailed cDNA for library amplification with 14 cycles of PCR ...
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bioRxiv - Cell Biology 2019Quote: ... pre-coated with fibronectin (Roche Cat# 11051407001) at 15,000 cells cells/well in 150 µL complete DMEM media (including 10% FBS) ...
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bioRxiv - Cell Biology 2019Quote: ... 7 μg/ml transferrin (Cat.# 652202, Roche) and 150 units penicillin/streptomycin (Cat.#15140) ...
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bioRxiv - Cell Biology 2019Quote: ... 15 μg/ml insulin (Cat.#1376497, Roche), 7 μg/ml transferrin (Cat.# 652202 ...
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bioRxiv - Cell Biology 2019Quote: ... and Phosphatase inhibitor (Roche Cat. No. 04906845001), according to the manufacturer instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... supplemented by protease inhibitors (Cat#11836170001, Roche) for 1h on ice ...
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bioRxiv - Microbiology 2021Quote: ... Kapa Pure beads (KappaBiosystems, Cat # KK8002, Roche) were applied for purification and size selection of DNA with a volumetric ratio of 0.6X-0.8X ...