Labshake search
Citations for Roche :
201 - 250 of 4954 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... The expression of LTK and ALK was determined using LightCycler 480 SYBR Green I Master Mix (Roche Life Science, Switzerland) and normalized to GAPDH using commercially available primers (Qiagen ...
-
bioRxiv - Developmental Biology 2019Quote: ... nkx2.4b expression in 28 hpf embryos was revealed by BM purple staining and tg expression in 55 hpf embryos by NBT/BCIP staining (Roche). Staining reactions were stopped by several 5 min washes in PBST followed by a 10 min incubation in 100% methanol to remove background staining ...
-
bioRxiv - Developmental Biology 2021Quote: ... Relative mRNA expression was quantified using FastStart SYBR Green master mix on the LightCycler 96 instrument (Roche Life Science, USA) with specific primer pairs (Supplementary Table 9).
-
bioRxiv - Cell Biology 2020Quote: ... 0.78 g/L CSM-His and 20 g/L agar) and screened for expression of Erg6-GFP by Western blot with anti-GFP bodies (11814460001; Roche) and for localization of Erg6-GFP to LDs by fluorescence microscopy.
-
bioRxiv - Genomics 2021Quote: ... Early passage cells were seeded at 25% confluency in six-well plates and transfected with 2 ug of expression vector using Fugene reagent (Roche). Cells were harvested and seeded into 100 mm dishes after 48 h of transfection ...
-
bioRxiv - Molecular Biology 2021Quote: A-431 cells were transiently hNAA40-flag transfected for 24 h with hNAA40-flag expression vector and using Fugene HD (Roche) according to the manufacturer’s instructions and as described above ...
-
bioRxiv - Cancer Biology 2022Quote: A vector control plasmid and WT and mutant Plk3 expression plasmids were transfected into 293T cells using FuGENE 6 transfection reagent (Roche) as described previously (Li et al. ...
-
bioRxiv - Microbiology 2023Quote: ... The coding sequence of SifA amplified from SL1344 genome were cloned in a series of prokaryotic expression vectors including pBH-HA from Roche, pET28a ...
-
bioRxiv - Physiology 2023Quote: ... Gene expression was measured after reverse transcription by quantitative real-time PCR (qRT-PCR) with FastStart SYBR Green master mix (Roche) using a LightCycler Nano or LC480 instruments (Roche) ...
-
bioRxiv - Physiology 2022Quote: ... The hairpin vectors were co-transfected with the lentivirus expression plasmid and packaging plasmid into actively growing HEK293FT using X-tremeGENE 9 DNA transfection reagent (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Mef2a mRNA expression was then assessed by qPCR (PowerTrack SYBR Green Master Mix, Applied Biosystems, LightCycler 480 Instrument II, Roche), along with that of 36b4 mRNA for data normalization ...
-
bioRxiv - Neuroscience 2024Quote: ... for all qPCRs except C9orf72 variant 2 expression levels for which TaqMan probes were used (sequences provided in Table S2) and measured with a LightCyclerTM (Roche). qPCR was performed in technical triplicate with data normalised to GAPDH expression levels.
-
bioRxiv - Cancer Biology 2023Quote: Gene expression levels were measured on a LightCycler 96 system using the LightCycler 480 SYBR Green I Master mix (Roche). The primer sequences were the following ...
-
bioRxiv - Physiology 2024Quote: ... Gene expression was measured by quantitative reverse transcription PCR (qPCR) using LightCycler 480 SYBR Green I Master (50-720-3180 Roche). Quantitative polymerase chain reaction (PCR ...
-
bioRxiv - Biochemistry 2021Quote: ... The expression of targeted genes and GAPDH transcripts were monitored by LightCycler®480 Real-time PCR (Roche Diagnostics GmbH, Germany) using gene-specific primers (Eurofins ...
-
bioRxiv - Cancer Biology 2022Quote: Gene expression knockdown in response to shRNA expression was verified by RT-QPCR as previously described (Saurty-Seerunghen et al 2019) using the LightCycler480 (Roche, France) and the SYBR Green PCR Core Reagents kit (Bimake.com) ...
-
bioRxiv - Molecular Biology 2019Quote: ... A qPCR analysis of mRNA expression from FD cells samples were conducted using KAPA SYBR Fast qPCR master mix (Kapa Biosystems) in a StepOne plus thermocycler PCR machine (Applied Biosystems ...
-
bioRxiv - Developmental Biology 2020Quote: ... The expression level of different genes was assessed with quantitative real-time PCR using the LightCycler Real-Time PCR system (Roche Diagnostics) and the primers described in the Supplementary data ...
-
bioRxiv - Physiology 2021Quote: ... Gene expression of the target genes fshb and lhb was quantified by qPCR using the Light Cycler 96 (Roche, Basel, Switzerland) thermocycler and SYBR Green I master (Roche ...
-
bioRxiv - Physiology 2021Quote: ... The expression of targeted genes and GAPDH transcripts were monitored by LightCycler®480 Real-time PCR (Roche Diagnostics GmbH, Germany) using gene-specific primers (Eurofins ...
-
bioRxiv - Plant Biology 2023Quote: ... The expression patterns of candidate genes were explored by Real-time fluorescence quantitative PCR using a Roche thermocycler (LightCycler480, Roche, Switzerland). The ACTIN2 (At3g18780 ...
-
bioRxiv - Cancer Biology 2023Quote: ... mRNA expression of melanocytic markers and PD-L1 was determined using intron-spanning primers with SYBR FAST qPCR master mix (Kapa Biosystems). qRT-PCR was performed with the following primers ...
-
bioRxiv - Plant Biology 2020Quote: ... Western Blotting was performed on 14 day old seedlings to determine protein expression using anti-HA high affinity antibody (Roche, Penzberg, Germany). Primer sequences for RxL21 and RxL21 deletion variants are in Table S9 ...
-
bioRxiv - Molecular Biology 2021Quote: ... were transiently transfected for 24 h with 9,36 μg of hNAA40-flag expression vectors and using 35 μl Fugene HD (Roche, cat. n°04709705001) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: Relative mRNA expression was determined by RT-qPCR using LightCycler® 480 SYBR Green I Master Mix on a LightCycler® 480 instrument (Roche). All reactions were performed in 10 or 20 µL volumes ...
-
bioRxiv - Pathology 2022Quote: ... Expression of mtDNA encoded genes were determined by quantitative real-time RT-PCR using SYBR Green PCR Master Mix (Roche Applied Science). The primers were listed as followed.
-
bioRxiv - Cancer Biology 2021Quote: CHI3L1 and nestin expression was detected using the ultraView DAB protocol on the automated VENTANA® BenchMark ULTRA platform (Roche, Basel, Switzerland).
-
bioRxiv - Cancer Biology 2021Quote: ... Quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) analysis of cDNA expressions was conducted using SYBR FAST Universal qPCR Master Mix (Kapa Biosystems; #KK4602) with a StepOnePlus real-time PCR system (Applied Biosystems) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The relative expression levels were determined by performing a quantitative reverse transcription PCR (qRT-PCR) analysis using a LightCycler 480 system (Roche, Basel, Sweitzer) with an Evo M-MLV RT-PCR kit (Accurate Biotechnology Co. ...
-
bioRxiv - Cell Biology 2024Quote: ... The expression levels of individual genes of interest were determined by quantitative real time PCR (qPCR, Roche Diagnostics, Germany or Stratagen, USA), primer pairs used for amplification are listed in Table 1 ...
-
bioRxiv - Cancer Biology 2023Quote: ... For gene expression analysis, appropriate primers (metabion international AG, Germany) and LightCycler® 480 SYBR® Green I Master (Roche, Mannheim, Germany) were used ...
-
bioRxiv - Microbiology 2020Quote: ... and the Library Quantification Kit – Illumina/Universal Kit (KAPA Biosystems) quantified the prepared libraries ...
-
bioRxiv - Genetics 2020Quote: ... PCR KIT (Roche). For each reaction ...
-
bioRxiv - Genomics 2022Quote: ... High Sensitivity DNA Analysis Kit and KAPA SYBR FAST qPCR Kit (Roche). WGS was performed on the HiSeq X (HCS HD 3.5.0.7 ...
-
bioRxiv - Cancer Biology 2022Quote: ... RNase free kit (Roche). RNA amount was quantified and cDNA was prepared using TaqMan Reverse Transcription Reagents (Applied Biosystems) ...
-
bioRxiv - Microbiology 2023Quote: ... followed by library preparation with KAPA RNA HyperPrep kit (Roche, kit code KK8541), according to manufacturer instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... using an Ovation Ultralow V2 kit (NuGEN) or a Kapa DNA HyperPrep Kit (Roche) in combination with IDT UD barcodes (Illumina) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Riboprobes were synthesized by DIG RNA labeling kit and in vitro transcription kit (Roche Applied science ...
-
bioRxiv - Cell Biology 2024Quote: ... Libraries were quantified using the KAPA Library Quanitification Kits - Complete kit (Universal) (Kapa Biosystems). Average size fragment was determined using a LabChip GXII (PerkinElmer ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5’/3’RACE Kit (Roche) was used to amplify the RNA obtained and the final DNA was sequenced to obtain the +1 nucleotide of str ...
-
bioRxiv - Molecular Biology 2020Quote: ... and SeqCap Adapter Kit (Roche) according to manufacturer instructions ...
-
bioRxiv - Microbiology 2019Quote: ... chemiluminescent kit (Roche Applied Science). Chemiluminescence values were obtained with the use of a TECAN at 492 nm ...
-
bioRxiv - Genetics 2020Quote: ... KAPA HyperPrep Kit (KAPA Biosystems), and AMPure XP Beads (Beckman Coulter ...
-
bioRxiv - Biochemistry 2020Quote: ... BrdU (colorimetric) kit from Roche Diagnostics (Mannheim ...
-
bioRxiv - Microbiology 2022Quote: ... The KAPA HyperPlus kit (Roche) was used to construct the genomic libraries with 10 ng of DNA ...
-
bioRxiv - Cancer Biology 2022Quote: (KAPA library quantification kit, Roche). The sequencing was carried out using paired-end mode (PE100 ...
-
bioRxiv - Developmental Biology 2022Quote: ... FLU RNA labeling kit (Roche) or DNP-UTP (Perkin Elmer).
-
bioRxiv - Immunology 2022Quote: Cytotoxicity Detection Kit (Roche CustomBiotech), which quantifies cytotoxicity/cytolysis based on the measurement of lactate dehydrogenase (LDH ...
-
bioRxiv - Developmental Biology 2022Quote: ... purified (PCR cleanup kit, Roche) and transcribed (SP6 mMessage mMachine Kit Ambion) ...
-
bioRxiv - Microbiology 2019Quote: ... and KAPA HyperPlus Kit (Roche) for Illumina sequencing ...