Labshake search
Citations for Roche :
1 - 50 of 4954 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... Yeast RNA (Roche) was included as the vehicle for RNA recovery.
-
bioRxiv - Biochemistry 2022Quote: ... Yeast tRNAmix (Roche) was used to reduce the number of compounds that inhibit PRORP1 activity by targeting tRNA ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1x yeast protease inhibitors (Roche) using a bead beater (Biospec products ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1x yeast protease inhibitors (Roche) using a bead beater (Biospec products ...
-
bioRxiv - Biochemistry 2023Quote: ... Total yeast RNA (totRNA, Roche) was resuspended in aggregation assay buffer (20 mM potassium phosphate pH 7.2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 µg/µL yeast RNA (Roche), 1 U/µL RiboLock RNase inhibitor (Thermo Scientific) ...
-
Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail.bioRxiv - Animal Behavior and Cognition 2021Quote: ... 200 μg/mL yeast tRNA (Roche Diagnostics), and 500 μg/mL DNA (Roche Diagnostics) ...
-
bioRxiv - Biochemistry 2022Quote: ... Baker’s yeast tRNAmix was purchased from Roche. Gambogic acid was purchased from EMD Millipore and juglone (5-hydroxy-1,4-naphthoquinone ...
-
bioRxiv - Physiology 2023Quote: ... 0.25 mg/mL yeast tRNA (Roche, 10109525001), 0.2 mg/mL fish sperm ssDNA (Roche ...
-
bioRxiv - Biochemistry 2021Quote: ... and 20 ug of competitor yeast tRNA (Roche) in a total volume of 300 uL per sample ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1 mg/ml yeast tRNA (Cat. # 10109509001, Roche), 1×Denhardt’s [1% (w/v ...
-
bioRxiv - Biochemistry 2024Quote: ... we mixed 0.6 µg of yeast tRNA (Roche), 10 mM MgCl2 ...
-
bioRxiv - Immunology 2022Quote: ... CAT expression was determined using an ELISA kit in accordance with the manufacturer’s instructions (Roche). Bovine IFN-α standards were used to construct a type I IFN standard curve to interpolate sera sample IFN levels.
-
bioRxiv - Biochemistry 2020Quote: ... using 10 ng / µl yeast tRNA as a carrier (Roche) and created NTC samples of the same dilution ...
-
bioRxiv - Cell Biology 2024Quote: ... MycTrap beads were pre-incubated with yeast tRNA (Roche, Switzerland) to block non-specific RNA binding in 0.15M KCl Wash Buffer supplemented with 0.5mM DTT and 40U/ml RiboLock RNase inhibitor (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2019Quote: For quantification of CAT expression we used an ELISA based assay (CAT ELISA Kit assay, Roche). Briefly ...
-
bioRxiv - Microbiology 2022Quote: ... Cell-clone colonies were tested for β-galactosidase expression to check viral infectivity (kit Roche #11758241001). Positive clones were transduced with the lentiviral vector (LV ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... α-SG expression analysis were performed using the TaqMan gene expression Master Mix (Roche), following the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... were generated cloning each enhancer from the genomic zebrafish DNA DNA using KAPA Long Range HotStart PCR kit (Kapa Biosystems) and cloned into the E1b:GFP:AC-DS vector (102417 Addgene (Chong-Morrison et al. ...
-
bioRxiv - Cancer Biology 2022Quote: ... Expression of the genes were determined using FastStart Essential DNA Green Master Kit (ROCHE, catalog no: 06402712001). cDNA converted from RNA was used as 1:5 dilution (in ultra-pure water ...
-
bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels were determined by qRT-PCR with KAPA SYBR FAST qPCR Master Mix Kit (Kapa Biosystems, KK4610). cDNA was synthesized from human cells using SuperPrep II Cell Lysis & RT Kit (TOYOBO ...
-
bioRxiv - Microbiology 2022Quote: ... 0.25 mg/ml yeast RNA and 1 % blocking reagent for nucleic acids (Roche, Switzerland) was used ...
-
bioRxiv - Genomics 2020Quote: ... mixed with alignment oligos and sample tracking control oligos (Nimblegen Hybridisation and Sample Tracking Control Kits) and hybridised to a 12×135k HD2 expression array (Roche Nimblegen ...
-
bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels were determined by qRT-PCR with KAPA SYBR FAST qPCR Master Mix Kit (Kapa Biosystems, no. KK4610). Relative expression levels were normalized to human ACTB or mouse Actb ...
-
bioRxiv - Microbiology 2019Quote: ... RT-qPCR analysis of cytokine related porcine gene expression was performed using the KAPA SYBR-FAST qPCR Kit (KAPA Biosystems), using the primers shown in Table 1 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Gene expression was detected using the LightCycler 480 SYBR Green I Master Mix or Probes Master Mix kit (Roche Diagnostics) after PCR (5 min at 95 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... Polymerase for cloning was obtained from Kapa Biosystems (Wilmington, MA, USA). pcDNA™3.1 (+ ...
-
bioRxiv - Molecular Biology 2022Quote: ... All cloning was performed using KAPA HiFi DNA Polymerase (Roche 07958846001), In-Fusion HD Cloning Kit (Clontech 639649) ...
-
bioRxiv - Developmental Biology 2022Quote: ... 1 mM sodium pyruvate and Hybridoma Fusion and Cloning Supplement (Roche) in a deep 10 cm plate (Fisher) ...
-
bioRxiv - Microbiology 2024Quote: All cloning was performed using KAPA HiFi DNA Polymerase (Roche 07958846001), In- Fusion HD Cloning Kit (Clontech 639649) ...
-
bioRxiv - Microbiology 2020Quote: ... The sgRNA expression cassettes were amplified from gDNA in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems). For PCR1 ...
-
bioRxiv - Cell Biology 2022Quote: ... Gene expression was analyzed by LightCycler 480 SYBR Green Ⅰ Master kit with LightCycler 480 Detection System (Roche Diagnostics, Mannheim, Germany). qPCR values were normalized by GAPDH expression ...
-
bioRxiv - Microbiology 2022Quote: ... Quantitative expression assays were performed by using the 2x M5 HiPer SYBR Premix EsTag kit (Mei5bio) and a LightCycle®96 (Roche) real-time PCR detection system ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... We detected expression using SYBRgreen marker (Roche, Germany). To obtain the normalized expression values of Tdrd7 in BMRrepr samples with respect to the wild-type condition ...
-
bioRxiv - Microbiology 2023Quote: ... Gene expression was quantified using SYBR Green (Roche) in a CFX96 or CFX384 (BioRad ...
-
bioRxiv - Molecular Biology 2023Quote: ... The USER cloning was done with KAPA HiFi Hotstart Uracil□+□Readymix (Roche) and USER compatible primers specified in Supplemental table 2 ...
-
bioRxiv - Neuroscience 2023Quote: ... All PCR based cloning steps were performed using KAPA HiFi taq (Roche) and verified by sequencing ...
-
bioRxiv - Microbiology 2024Quote: DNA amplification for cloning was made by KAPA HiFi HotStart ReadyMix (Roche) according to manufacturer instruction ...
-
bioRxiv - Neuroscience 2022Quote: ... and PlxnA4 expression levels were measured by qRT-PCR reaction using KAPA SYBR FAST ABI prism qPCR kit (Kapa Biosystems, catalog #KK4605). Primers (5’3’ ...
-
bioRxiv - Plant Biology 2021Quote: Gene expression analysis via RT-qPCR was performed using a LightCycler480 instrument and LightCycler480 SYBR Green Master Kit reagents (Roche, Indiana, IN). Relative gene expression levels were determined using a standard curve method ...
-
bioRxiv - Bioengineering 2022Quote: ... Gene expression level was quantified using SYBR Green (Roche) with QuantStudioTM 5 System (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... Gene expression was determined using FastStart SYBR-green (Roche) following the quantitative polymerase chain reaction (qPCR ...
-
bioRxiv - Immunology 2024Quote: ... Gene expression was quantified using FastStart Roche SybrGreen (Roche), the SteponePlus™ Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.05M NaCl) and once with solution B (0.1M NaCl) and then blocked with yeast RNA (Roche, 10109223001). The blocked C1 beads ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 µl of target RNA were incubated for 15 min with 0.6 µg baker’s yeast tRNAPhe (Roche), 1 mM MgCl2 and increasing amounts of protein in a volume of 20 µl ...
-
bioRxiv - Molecular Biology 2019Quote: ... and CAT expression was quantified using the CAT ELISA (Roche) as per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: Gene expression was quantified by qPCR on Lightcycler 480 (Roche). Briefly ...
-
bioRxiv - Developmental Biology 2023Quote: ... Gene expression quantification proceeded with a SYBR Green Mastermix (Roche Diagnostics LightCycler 480 SYBR Green I ...
-
bioRxiv - Developmental Biology 2021Quote: ... Fixed embryos were placed into hybridization buffer (50% formamide, 5×SSC, 1 mg/ml yeast tRNA (Roche, 10109223001), 100 μg/ml heparin (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: ... and then prehybridized (50% formamide [ThermoScientific 15515026], 5X SSC [ThermoScientific AM9763], 1X denhardt’s solution [Sigma D2532], 250ug/mL Brewer’s yeast tRNA [Roche 10109495001] ...