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Citations for Roche :
451 - 500 of 5243 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... containing protease inhibitor cocktail (11697498001, Roche) and phosphatase inhibitor cocktail (4906845001 ...
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bioRxiv - Immunology 2023Quote: ... containing 1mg/mL dispase II (Roche). The separated epidermis was then laid upon cell culture medium (RPMI-1640 medium supplemented with 10% fetal calf serum ...
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bioRxiv - Microbiology 2023Quote: ... containing 10 μM oseltamivir carboxylate (Roche) and 10 µM zanamivir (GSK ...
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bioRxiv - Cell Biology 2023Quote: ... containing complete mini protease inhibitors (Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: ... DIGprobes were detected with antibodies in MABT containing 5% horse serum appropriate for NBT/BCIP in situ hybridization (Roche anti-DIG-AP 1:1000) or for FISH (Roche anti-DIG-POD 1:1000) ...
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bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS containing 0.5% Tween-20 samples were incubated with fluorescent labelled secondary antibodies containing 4′,6-diamidino-2-phenylindole (DAPI; Roche Cat# 10236276001, 1.0 µg/ml) for 30 min ...
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bioRxiv - Developmental Biology 2019Quote: ... to visualise targeted cells we performed immunocytochemistry using anti-fluorescein antibodies (Roche 426346910).
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bioRxiv - Developmental Biology 2022Quote: ... Cell death was revealed using the alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche) and NBT/BCIP (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Pellets containing virions and EVs were resuspended in 1 ml PBS containing a protease cocktail inhibitor (Roche) during 2 h at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: Cells were lysed with ice-cold RIPA lysis buffer containing EDTA-free protease inhibitor cocktail and PhosSTOP phosphatase inhibitor (Roche), with DNA-shearing by 21-gauge needle ...
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were harvested with a cell scraper and lysed by adding 2 x TNI buffer 8 containing complete Ultra protease inhibitor (Roche) and Halt phosphatase inhibitor (Pierce) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed with ice-cold DPBS and scraped in ice-cold kit lysis buffer (1 mL/107 cells) containing a cocktail of protease inhibitors (cOmplete, EDTA-free; Roche). Cells were then lysed in a Potter-Elvehjem homogenizer (~50 strokes) ...
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bioRxiv - Microbiology 2021Quote: ... each tube contained about 106 of cells in 200 μl of the digestion buffer containing 50 μU of neuraminidase (Roche). The tubes were incubated at 37°C for one hour ...
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bioRxiv - Cancer Biology 2020Quote: Total cellular protein was isolated from exponentially growing cells and lysed in ice cold RIPA buffer containing 1x cOmplete Mini Protease Inhibitor Tablets (Roche). Protein concentration were quantified and 75 ug resolved (SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately eight million cells were harvested for each sample and lysed into Pierce lysis buffer (Thermo) containing Complete protease inhibitors (Roche). Lysate was clarified by centrifugation at 10,000 g for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... At 80% confluency the cells were harvested on ice by scraping and washed once with PBS containing protease inhibitors (Roche). The cells were pelleted at 300g for 5 min at 4°C and were washed once with KPBS buffer (136 mM KCL ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were briefly washed in ice-cold PBS and lysed in RIPA buffer containing phosphatase inhibitor cocktail (PhosStop, Roche, USA) and protease inhibitor cocktail (HaltProtease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2021Quote: 160 μl DMEM containing 10 % FBS were added to the bottom wells of xCELLigence Real-Time Cell Analyzer CIM plate (Roche), as chemo-attractant ...
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bioRxiv - Cell Biology 2021Quote: ... Cells lysed with lysis buffer were incubated in the buffer containing protease and phosphatase inhibitor cocktails (04693159001 and 04906845001, Roche), 1 mM serine protease inhibitor PMSF (phenylmethylsulfonyl fluoride ...
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bioRxiv - Cell Biology 2020Quote: Protein lysates were obtained from tissues or cells using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Roche, USA). Western blotting was conducted as described previously (Yao et al. ...
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bioRxiv - Microbiology 2022Quote: ... UK) at 1kbar (10g of cells in 40ml final of 20mM Tris pH 8.0 containing protease inhibitor (Roche, EDTA free), at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Protein aggregation was evaluated by western blot in total cell extracts prepared in 1% Triton X-100 in PBS containing proteases and phosphatases inhibitors (Roche). Protein quantification was performed with the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: ... Labelled cells were washed twice with 1mL FACS buffer and resuspended in 500uL FACS buffer containing DNAse I (10ug/mL, Roche). Sorting of the cells was performed into 500uL RNAlater on FACSAria III (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed with cold phosphate buffered saline (PBS) then scraped into cold PBS containing protease inhibitors (Roche, Laval, QC) and 5 mM sodium fluoride (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... The washed pellet was resuspended in approximately 1/3 of packed cell volume of ORC lysis buffer containing cOmplete Protease Inhibitor Cocktail Tablet (1 tablet per 25 mL total volume; Roche) and frozen dropwise in liquid nitrogen.
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bioRxiv - Neuroscience 2020Quote: ... Cells were dissociated into a single-cell suspension by incubating the retinas in 1mL of HBSS containing 10U of papain (Roche) and 200µM cysteine ...
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bioRxiv - Neuroscience 2019Quote: ... cells were washed with cold PBS and lysed on ice with RIPA buffer containing complete mini protease inhibitor cocktail (Roche), followed by bath ultrasonication in chill water and centrifugation for total protein extract ...
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bioRxiv - Cell Biology 2019Quote: Lysates from A375 cells stably expressing FLAG-EZH2 with or without Dox-inducible expression of FLAG-USP7 were prepared by incubating the cells in lysis buffer containing protease inhibitor cocktail (Roche). Anti-FLAG immunoaffinity columns were prepared using anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Genetics 2019Quote: Protein was isolated from 10 brain-imaginal disc complexes were dissected from wandering L3 larvae or S2 cells and homogenized in 1X Laemmli/RIPA buffer containing 1X protease inhibitors (Roche cOmplete Mini EDTA-free protease inhibitor tablets ...
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bioRxiv - Microbiology 2019Quote: Whole cell lysates were prepared by suspending purified parasite pellets in whole cell lysis buffer (1XPBS, 1% v/v Triton X-100) containing Protease Inhibitors (cOmplete EDTA-free, Roche). 24 h in vitro ookinetes were also subjected to cellular fractionation using the following method ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were harvested in ice-cold PBS using a cell scraper and lysed by sonication for three times 10 seconds in ice-cold PBS containing Complete protease inhibitors (Roche). Unbroken cells were removed by centrifugation at 500 g for 2 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Whole-cell lysates were prepared by using Triton-X sample buffer containing protease inhibitor cocktail from Roche (Grenzach-Wyhlen, Germany). The protein concentration was assessed by using the DC Protein assay reagent obtained from Bio-Rad Laboratories ...
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bioRxiv - Genetics 2021Quote: ... cells were collected in lysis buffer (TBS + 1% Triton X-100) containing protease inhibitors (Complete Mini EDTA-Free, Roche 04693159001), briefly sonicated ...
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bioRxiv - Neuroscience 2020Quote: ... Protein aggregation was evaluated by western blot in total cell extracts prepared in 1% Triton X-100 in PBS containing proteases and phosphatases inhibitors (Roche). Sample quantification was performed with the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested by centrifugation and lysed in 1% Nonidet P40/1 mM EDTA/PBS containing Complete protease inhibitors (Roche), and nuclei were removed by centrifugation ...
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bioRxiv - Genomics 2022Quote: ... The reaction was quenched using 125 mM glycine for 5 minutes with rotation and the cells were washed three times using ice-cold PBS containing complete protease inhibitor cocktail tablets (Roche). Cells were resuspended in lysis buffer (1% SDS ...
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bioRxiv - Immunology 2021Quote: ... HEK293T and A549 cells were lysed in ice-cold RIPA lysis buffer containing complete protease inhibitor and phosphatase inhibitor cocktails (Roche), resolved by SDS-PAGE ...
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bioRxiv - Genetics 2022Quote: ... with 5mM EDTA to remove waste and epithelial cells then were minced and dissociated in RPMI containing collagenase (100 mg/ml collagenase A; Roche), DNase I (50 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: Whole endothelial extracts were prepared from 70-80% confluent cells in lysis buffer (3% SDS, 60 mM sucrose, 65 mM Tris (pH 6.8) containing protease inhibitor cocktail (Roche-11836170001). Protein extracts were sonicated briefly and the concentration of the isolated proteins was assessed using BCA Protein Assay Reagent (Thermo Scientific™ ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were lysed by the lysis buffer 10 M urea containing the cOmplete™ protease inhibitor cocktail (Roche, #11697498001). The cell tubes were ultrasonicated at 4 ℃ for two cycle (1 min per cycle ...
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bioRxiv - Genetics 2022Quote: ... 50000 cells were lysed in NPB buffer (PBS containing 5% BSA, 1mM DTT, 0.2% IGEPAL, 1□×_Roche Complete Protease Inhibitor) and incubated on ice for 15 min to isolate nuclei ...
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bioRxiv - Neuroscience 2022Quote: ... washing them twice with PBS containing 1 mM CaCl2 and MgCl2 and collecting cells by scraping them in CaCl2/MgCl2 PBS containing cOmplete protease inhibitor cocktail (Roche). Lysates were cleared by centrifuging at 1000 x g for 10 min at 4 °C and pellets were resuspended in PBS with 1 % Igepal ...
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bioRxiv - Microbiology 2022Quote: ... 33.75 µL cells were mixed with a PCR master mix containing 37.5 µL KAPA HiFi HotStart ReadyMix (Roche, CN KK2602), 2.25 µL second strand synthesis primer SMRT_dT (10 µM ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T-cells were plated in 200ul of medium (RPMI, 10% human serum) containing IL-2 (Roche, 10 IU/mL) and IL-7 (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: Whole cell lysate proteins were extracted using RIPA buffer (Cat. #89901, Pierce) containing complete protease inhibitor cocktails (Cat. #04693116001, Roche) and phosSTOP (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were selected 48 h after transduction with antibiotic supplemented media containing either 0.1 mg/mL Hygromycin B (Roche, H3274), 5 μg/mL Blasticidin S Hydrochloride (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using a 1% triton X-100 containing lysis buffer (CST) supplemented with protease inhibitor cocktail (Roche) on ice for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were extracted by resuspending cells from 6-well plates in 100 μl of RIPA or 7M Urea Buffer containing protease inhibitor (11873580001, Roche) and ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed 80 h post seeding in RIPA buffer (Thermo, 89901) containing cOmplete EDTA-free protease inhibitor cocktail (Roche). Lysate were centrifuged at 15000 rpm for 15 min ...