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Citations for Roche :
101 - 150 of 3318 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... size and concentration of cDNA libraries were checked using the High Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480). Libraries were sequenced using an Illumina Novaseq 6000 sequencer as single-end 100 base reads ...
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bioRxiv - Genomics 2020Quote: ... cDNA libraries were prepared using 500 ng of large RNA as input for the KAPA mRNA HyperPrep Kit (Kapa Biosystems; Wilmington, MA, USA) combined with the KAPA Unique Dual-Indexed Adapter Kit (Kapa Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... 10mM β-mercaptoethanol) and two protease inhibitor tablets (Roche) added ...
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bioRxiv - Biochemistry 2021Quote: ... with two complete EDTA-free protease inhibitor tablets (Roche). Cells were clarified by centrifugation ...
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bioRxiv - Immunology 2021Quote: ... cDNA was generated using High-capacity cDNA generation kit (Roche). PCR amplicons ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was produced with Transcriptor First-strand cDNA kits (Roche), and RT-PCR was performed using Kappa SYBR Green 2X Mastermix.
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were quantified by KAPA Library Quantification Kit (Roche), normalized ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were quantified using KAPA Library Quantification Kit (Roche) following the manufacturer’s instructions and by BioAnalyser ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were then quantified by Kappa Library Quantification (Roche), pooled ...
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bioRxiv - Cell Biology 2023Quote: ... Libraries were quantified using KAPA library quantification kit (Roche) and 20nM of libraries sequenced by paired end sequencing (150bp ...
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bioRxiv - Microbiology 2023Quote: ... were ligated to the individual cDNA products to barcode the samples and Kapa HiFi HotStart Library Amplification kit (kk2612, Roche Sequencing and Life Science) was used for library amplification.
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bioRxiv - Zoology 2023Quote: ... Paired-end cDNA libraries were constructed from 4 μg of total RNA using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, Inc., Wilmington, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Resultant libraries and barcoded hashtag libraries were quantified using the KAPA Library Quantification Kit (KAPA Biosystems). Primer sequences used for this study were shown in Supplementary Table 3 ...
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bioRxiv - Microbiology 2020Quote: ... Probe-target hybrids were visualized by a chemiluminescent assay with the DIG luminescent detection kit (Roche Molecular Biochemicals), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... and the cDNA generated using the Transcriptor Universal cDNA Master (Roche) following manufacturer’s protocols ...
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bioRxiv - Physiology 2021Quote: ... and cDNA prepared using Transcriptor High Fidelity cDNA Synthesis Kit (Roche). Relative abundance of cDNAs was determined a Roche LightCycler 480 ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated with Evo-Script Universal cDNA Master kit (Roche) and qPCR performed using SYBR Green I Master kit on LightCycler 480 instrument(Roche) ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA was synthesized with Transcriptor high-fidelity cDNA synthesis kit (Roche) and RT-qPCR was performed using Luminaris HiGreen qPCR Master Mix (Thermo scientific ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was generated using Transcriptor First-strand cDNA synthesis kit (Roche) and ProtoScript II first strand cDNA synthesis kit (New England BioLabs ...
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bioRxiv - Plant Biology 2021Quote: ... and two tablets of EDTA-free protease inhibitor cocktail (Roche). Unless otherwise stated ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were quantified by KAPA library quantification kit (KAPA biosystems) and then multiplexed and sequenced on Illumina Hiseq 4000 platform to obtain 150 nt paired-end reads.
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bioRxiv - Genomics 2019Quote: ... All libraries were quantified by Library Quantification Kit (Kapa Biosystems). The pools were sequenced using TruSeq SBS Kit v3-HS (Illumina Inc.) ...
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bioRxiv - Microbiology 2020Quote: ... and libraries prepared using a DNA KAPA library kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Libraries were quantified with the KAPA Library Quantification kit (Roche) and sequenced on a NovaSeq 6000 system (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... The final library was quantified (KAPA Library Quantification Kit, Roche) and sequenced (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Final libraries were quantified using KAPA Library Quantification Kits (Roche). The libraries were loaded at a concentration of 10 nM and sequenced on NovaSeq 6000 system (Illumina ...
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bioRxiv - Genomics 2022Quote: ... using the KAPA Library Quantification Kit for Illumina Libraries (Roche), and its profile was assessed using a DNA High Sensitivity LabChip kit on an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Libraries were quantified using a KAPA Library Quantification Kit (Roche Sequencing and Life Science ...
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bioRxiv - Systems Biology 2024Quote: ... Libraries prepared using the KAPA Library Preparation Kit (KAPA Biosystems) and amplified using NEB indexed adapters with 12 PCR cycles (15 sec at 98°C ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA synthesis was performed using Transcriptor High-Fidelity cDNA Synthesis Kit (Roche) with 500 ng RNA per reaction ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by cDNA synthesis using Transcriptor First Strand cDNA Synthesis Kit (Roche) then was stored at −20 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... and cDNAs transcribed with the Transcription First Strand cDNA Synthesis Kit (Roche) according to the manufacturer’s description ...
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bioRxiv - Molecular Biology 2020Quote: ... and cDNA synthesised using the Transcriptor First Strand cDNA Synthesis kit (Roche) with random hexamer primers ...
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bioRxiv - Genomics 2020Quote: ... Double stranded cDNA was synthesised using the cDNA Synthesis System (Roche-Nimblegen), including RNase I and Proteinase K treatment followed by DNA clean up using the PCR purification kit (Qiagen) ...
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Development of a novel allograft model of prostate cancer: a new tool to inform clinical translationbioRxiv - Cancer Biology 2020Quote: ... and phenol-chloroform extraction and cDNA synthesised using first strand cDNA (Roche) according to the respective manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... and cDNA was synthesized using Transcriptor First Strand cDNA Synthesis Kit (Roche) following the protocol recommended by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... and cDNA was synthesized using the Transcriptor Universal cDNA Master kit (Roche). Abca1 ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesised with the Transcriptor First Strand cDNA Synthesis kit (Roche). Gene expression was determined using SYBR-green qPCR on the Lightcycler 480 (Roche) ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was prepared with the Transcriptor High Fidelity cDNA Synthesis Kit (Roche) using oligo-dT primers ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA synthesis was performed using EvoScript Universal cDNA Master (Roche, cat#07912374001). The strawberry Elongation factor 1 (EF1 ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared using the Transcriptor Universal cDNA Master (Roche, Cat# 05893151001) in a 20 μl reaction containing 1x Transcriptor Universal Reaction Buffer ...
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bioRxiv - Immunology 2023Quote: ... and transcribed into cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche). SYBR green master mix (Roche) ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was then transcribed using the first strand cDNA synthesis kit (Roche), followed by PCR to amplify the fragments of interest using primer pairs listed in the key resource table ...
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bioRxiv - Systems Biology 2023Quote: ... cDNA was synthetized using the Transcriptor First Strand cDNA Synthesis Kit (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized using a Transcriptor First Strand cDNA Synthesis Kit (Roche). Amplification was performed using the Tks Gflex DNA Polymerase Low DNA (Takara Bio) ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized using the Transcriptor First Strand cDNA synthesis kit (Roche) using total RNA ...
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bioRxiv - Genomics 2020Quote: ... Library concentration was determined using a KAPA Library Quantification Kit (Roche). Deep sequencing was performed on an Illumina NovaSeq 6000.
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bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared using the KAPA Library Preparation Kit (KAPA Biosystems), and were amplified for a total of 12 PCR cycles (15 s at 98°C ...
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bioRxiv - Genetics 2020Quote: ... Sequencing libraries were quantified using qPCR Library Quantification kits (Roche, #KK4824).
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared using the KAPA HTP Library preparation kit (Roche), according to the manufacturer’s instructions ...