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Citations for Roche :
101 - 150 of 5877 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... and Ki-67 was performed automatically on a Ventana Benchmark XT autostainer system with the XT ultra-View DAB Kit (Ventana Medical Systems, Roche, Basel, Switzerland). Primary antibodies were the monoclonal anti-PD-L1 antibody raised in rabbit (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System, Laval, QC, Canada) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by two washes with Buffer A (without Roche cOmplete protease inhibitors). Diluted Chromatin was added to the pre-bound beads and incubated for 4 h on a rotating wheel with 30 rpm at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue was homogenized in two 10-second intervals using MagNA Lyser (Roche), incubated with 200 ul chloroform (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: All specimens were tested concurrently using two cobas Liat Analyzer (Roche Diagnostics) instruments and a LIAISON MDX (Diasorin Molecular ...
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bioRxiv - Cell Biology 2024Quote: ... two steps of transfections were performed: DNA using XtremeGENE™ 9 (Roche) followed by siRNA (4392420-s30722 ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunostaining was carried out using the BenchMark XT (Ventana Medical Systems, Illkirch Graffenstaden, France) with the OmniMap kit (a “biotin-free” system using multimer technology, Roche, Boulogne Billancourt, France) and a Tris borate EDTA pH8 buffer for antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... OptiView DAB detection kit (proprietary, indirect, biotin free system, Roche Tissue Diagnostics, Indianapolis, IN) was used for primary antibody detection ...
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bioRxiv - Genetics 2021Quote: ... Two corneas (per mouse) were incubated in 15 mg/ml Dispase (4942078001, Roche) in DMEM (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... kit according to the manufacturer’s instructions on a LightCycler 96 Real-Time PCR System (Roche).
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bioRxiv - Cell Biology 2021Quote: ... The Optiview DAB IHC detection and the Discovery Chromomap DAB Kits (Roche, Ventana Medical Systems) were used to stain with the antibodies against Glut1 and SF3B1 ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was extracted using the Roche High Pure RNA isolation kit (Roche Molecular Systems, Inc.) according to the manufacturer protocol and purity as well as integrity controlled using the Agilent RNA 6000 nano kit (Agilent Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... which was sequenced (Roche 454 Genome Sequencer FLX system with a Titanium kit, Roche Diagnostics). Sequencing was done at the Functional Genomics Center Zürich ...
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bioRxiv - Physiology 2020Quote: ... and immunodetection of the DIG-labeled hybrids was performed with a peroxidase-conjugated anti-DIG antibody (1:2000; raised in sheep; Roche Diagnostics, Mannheim, GER). The fluorescence signal was developed with tyramide signal amplification (TSA ...
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bioRxiv - Molecular Biology 2021Quote: ... washed two times with PBS and blocked with 1.5% blocking reagent (Roche Applied Science) at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then washed two times with cold PBS containing protease inhibitor cocktail (Roche) followed by lysis with 500 μl ChIP sonication buffer (0.5% SDS ...
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bioRxiv - Cancer Biology 2019Quote: ... Two molecules were used: gefitinib (IRESSA®, Astra-Zeneca) and erlotinib (TARCEVA®, Roche). These two therapies were given until progression ...
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bioRxiv - Biochemistry 2022Quote: ... The DBD was amplified in two stages with Kapa HiFi DNA Polymerase (KAPA Biosystems) for tailed amplicon sequencing ...
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bioRxiv - Genetics 2023Quote: ... At least two libraries were independently prepared with the Expand Long Template polymerase (Roche) using the primers listed in Supplementary Table 9 ...
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bioRxiv - Biophysics 2023Quote: ... Two 1L pellets were thawed and one tablet of protease inhibitor (cOmplete™, Roche) was added ...
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bioRxiv - Neuroscience 2023Quote: ... followed by the detection with the ultraView Universal DAB Detection kit (Ventana Medical System Inc., Roche), according to the automated procedure ...
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bioRxiv - Cell Biology 2019Quote: ... Two ml of ice-cold lysis buffer supplemented with protease inhibitors (Roche Applied Science, 11836145001), phosphatase inhibitors (Calbiochem ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We performed a two-steps PCR protocol on a LifeCycler® 480 (Roche Life Science), using the following program ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 0.1 mM PMSF and two EDTA free protease inhibitor tablets (Roche, Basel, Switzerland), the expressed protein was extracted from the supernatant using PierceTM High-Capacity Ni-IMAC Resin (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 20 ml PBS plus two cOmplete Mini EDTA- free protease inhibitor tablets (Roche) and 25 μg/ml phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Pathology 2021Quote: ... CK values were determined using the CKL ACN 057 kit on a Cobas 311 Analyser System (Roche).
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bioRxiv - Cell Biology 2022Quote: ... Libraries were prepared using the Ovation RNA-seq System V2 (NuGen) and Hyper Prep Kit (Kapa Biosystems). Library quality and concentration evaluated using D5000 ScreenTape assay (Agilent ...
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bioRxiv - Microbiology 2019Quote: ... parasite cultures were treated for two weeks with the recommended working concentration of BM-Cyclin (Roche). M ...
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bioRxiv - Genomics 2020Quote: ... qPCR was performed in two technical replicates with the FastStart Essential DNA Green master mix (Roche) on a LightCycler 96 (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... Expression profiles were tested in triplicate on at least two mice on an LC480 instrument (Roche). Data were analyzed using the comparative Ct (ΔΔCt ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) with the addition of two EDTA-free cOmplete protease inhibitor cocktail (Roche, 11836170001). Supernatant from lysed bacteria was collected by centrifugation at 16000 g for 10 minutes at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Whole-mount two-color fluorescence ISH was performed using anti-DIG and -fluorescein POD antibodies (Roche) and Tyramide Signal Amplification (TSA ...
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bioRxiv - Biophysics 2019Quote: ... The two ends of the DNA fragments were ligated to digoxigenin-coated (Roche Diagnostics, Basel, Switzerland) or biotin-coated (Roche Diagnostics ...
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bioRxiv - Microbiology 2020Quote: ... RNA pellets were then resuspended in 200 μl H2O and subjected to two consecutive DNaseI (Roche)-treatments for 1h at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... cell pellets were thawed and mixed with two cOmplete ETDA-free protease inhibitor cocktail tablets (Roche) and 50 μL of benzonase (Molecular Biology
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bioRxiv - Cancer Biology 2023Quote: ... the cells were incubated with two lysis buffers containing a complete protease inhibitor cocktail (Roche, 11697498001) (Paro Rinse 1 ...
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bioRxiv - Neuroscience 2023Quote: ... and two sets of primers for WPRE or hGHpA genes using LightCycler 480 (Roche, Basel, Switzerland). The final titration of the AAV was estimated as relative quantitation according to a calibration curve calculated from the known numbers of copies of AAV plasmids.
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bioRxiv - Microbiology 2021Quote: The RNA was subjected to reverse transcription and second strand synthesis using the cDNA synthesis system kit (Roche). The acid nucleic quantification (RNA and cDNA ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time PCR reactions were performed with SYBR Green I Master Kit and LightCycler 480 system (Roche). mRNA levels of Col4a1 were normalized to the housekeeping gene Actb or Rpl32 ...
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bioRxiv - Microbiology 2024Quote: ... in a LightCycler® 480 system (Roche Molecular System), using primers and probes targeting the M gene of influenza A (sequences available upon request ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative polymerase reactions were performed in two technical replicates for each sample using LightCycler 450 instrument (Roche).
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bioRxiv - Molecular Biology 2019Quote: ... The levels were normalized to two housekeeping genes (Actb, Gapdh) amplified with SYBR Green Master Mix (Roche), as reported before (22).
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bioRxiv - Neuroscience 2023Quote: ... with a two-step cycling protocol was used for the gene expression analysis using LightCycler 480 (Roche). Using the comparative ΔCt method (ΔΔCT method) ...
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bioRxiv - Microbiology 2023Quote: ... pH 4.3) containing two dissolved cOmpleteTM EDTA-free protease inhibitor cocktail tablets (Roche Applied Science, Penzberg, Germany) and vacuum-infiltrated at 900 mbar for 10 min ...
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bioRxiv - Immunology 2020Quote: ... genes of SARS-CoV-2 was performed using the LightMix Modular SARS and Wuhan CoV E-gene Kit (TIB MOBIOL, Synheselabor GmbH, Berlin, Germany) in a LightCycler 480 II system following the manufacturer’s recommendation (Roche Diagnostics International Ltd. ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using a Roche LightCycler 480 system with a SYBR Fast qPCR Kit (KAPA Biosystems, KK4601). The expression levels of the target genes were normalized against that of tomato ACTIN2 ...
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bioRxiv - Genomics 2023Quote: ... EM-seq and WGBS libraries were quantified using the KAPA library quantification kit (KK4854; Roche Molecular Systems, Pleasanton, CA) and pooled in equimolar amounts ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using a One-step BrightGreen RT-qPCR Kit (Abm, G471-S) following the manufacturer’s protocol in the LightCycler 96 Real-Time PCR System (Roche). Isocitrate dehydrogenase (ICDH ...
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bioRxiv - Cancer Biology 2024Quote: ... 384 system (Roche). The relative expression level of each gene was calculated by the 2-△△Ct method with GAPDH as the reference gene ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole and 10 mM β-mercaptoethanol supplemented with DNAse I and two protease-inhibitor tablets (Roche). After a 30-min incubation ...