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Citations for Roche :
51 - 100 of 5877 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Approximately 500ng of FFPE RNA or 100ng of fresh frozen RNA per sample were used for RNA library construction using the KAPA RNA Hyper library prep kit (Roche, Switzerland) per the manufacturer’s instructions with minor modifications ...
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bioRxiv - Genomics 2020Quote: ... a total of 1.0 μg of extracted DNA was used as the starting material for PCR-free library construction (KAPA HyperPrep PCR-Free Library Prep kit; Roche, #KK8505); libraries were then mechanically sheared (Covaris microtube system ...
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bioRxiv - Molecular Biology 2023Quote: ... Library construction of 300 ng total RNA for each sample was made using KAPA Stranded mRNA-Seq Kit (Illumina) (Kapa Biosystems), using 10 cycles of PCR amplification ...
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bioRxiv - Microbiology 2023Quote: We used genomic DNA extracted by physical disruption for shotgun library construction with a KAPA Hyper Prep kit for Illumina (KAPA Biosystems)69 ...
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bioRxiv - Neuroscience 2023Quote: A total of 1.0 µg of extracted DNA was used for PCR-free library construction using the KAPA HyperPrep PCR-Free Library Prep kit (Roche, KK8505). Mechanical shearing using the Covaris microtube system (Covaris ...
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bioRxiv - Microbiology 2020Quote: ... Probe-target hybrids were visualized by a chemiluminescent assay with the DIG luminescent detection kit (Roche Molecular Biochemicals), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantification of library construction was performed using KAPA qPCR (Kapa Biosystems KK4824). The Illumina HiSeq4000 and NovaSeq6000 platform was used to achieve an average of 50,000 reads per cell ...
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bioRxiv - Cancer Biology 2019Quote: ... mRNA capture and construction of stranded mRNA-Seq libraries were made using KAPA mRNA HyperPrep Kit according to instructions (Roche Sequencing Solutions). NGS was performed at the Whitehead Genome Technology Core (HiSeq 2500 ...
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bioRxiv - Plant Biology 2022Quote: ... The enriched biotinylated DNA fragments were subjected to library construction on beads using the KAPA HyperPrep Kit for Illumina (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Paleontology 2022Quote: DNA library construction for whole-genome sequencing was performed using the KAPA Hyper Prep Kits for Illumina® NGS platforms (KAPA Biosystems, KK8504). Adapters and 8bp index oligos purchased from IDT® (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The purified DNA was used for Illumina sequencing library construction using a KAPA Hyper Prep Kit (for Illumina) (KAPA Biosystems, Wilmington, MA, USA). Sequencing was performed on an Illumina HiSeq 2500 platform (San Diego ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by two steps of DNA amplification by utilizing KAPA HyperPrep Kit (Roche KK8504). Then ...
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bioRxiv - Cell Biology 2022Quote: ... Using a two-step nested PCR with KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems), sgRNA expression cassettes were amplified ...
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bioRxiv - Genomics 2023Quote: ... To correct ONT reads another library using the same HMW sample as above was constructed for shotgun sequencing with the Hyper Library construction kit from Kapa Biosystems (Roche, Basel, Switzerland) and sequencing was performed using an Illumina NovaSeq at the Roy J ...
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bioRxiv - Cancer Biology 2021Quote: Doubling times were calculated using two different methods: (i) Impedance measurements were assessed using the RTCA system (Roche) by seeding at different cell densities and registering impedance signals every 20 min ...
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bioRxiv - Genomics 2021Quote: ... cDNA synthesis from two biological replicates was performed using the Transcriptor High-fidelity cDNA synthesis kit (Roche) and real-time PCR using SYBR green (Roche ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Two µg of total RNA were retro-transcribed using the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Science ...
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bioRxiv - Cell Biology 2022Quote: DNA sequencing by NGS consisted of two PCR amplification steps with KAPA HiFi HotStart ReadyMix PCR Kit (Roche). The first step (18 cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... The “Expend Long Template PCR System” kit (Roche) was used using 300 ng of the 4C library following the manufacturer’s instruction ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with two protease inhibitor tablets (Roche) and a scoop of DNAse ...
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bioRxiv - Biochemistry 2021Quote: ... Two Complete EDTA-free inhibitor tablets (Roche), 500 μL of a 100 mM PEFA solution and 40 mg of lysozyme were added to the cell suspension ...
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bioRxiv - Molecular Biology 2022Quote: ... two PCR reactions were performed with PCR anchor primer (included in the 2nd Generation 5’/3’ RACE Kit, Roche) and GB3 (oligo 61 ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR amplification was performed in two technical replicates using KAPA Hifi Hotstart PCR kit (Kapa Biosystems, MA, USA) via a Bio-Rad CFX96 thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We prepared dual-indexed sequencing libraries using the KAPA Hyper Prep Library Construction Protocol (KAPA biosystems, Wilmington, MA, USA), generally following the manufacturer’s protocol except that most steps were performed at one-quarter volume to save costs ...
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bioRxiv - Cell Biology 2020Quote: ... while chemistry samples collected by Alexandria University as well as all samples for immunoturbidimetry and PTH from the two centers were measured on Cobas 6000 modular system (Cobas modular Roche, Indianapolis IN, USA), Roche ...
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bioRxiv - Plant Biology 2019Quote: ... The resulting cDNA:RNA hybrids were treated with 10 U of DNase I (Roche, Basel, Switzerland) for 30 min at 37°C and purified on PCR clean-up column (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2020Quote: ... two EDTA-free protease inhibitor cocktail tablets (Roche), 0.2% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... two different platforms (CFX96 and LC480 I, (Roche)) and deviating primer (optimised and −33,3% (i.e ...
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bioRxiv - Biochemistry 2021Quote: ... Two KAPA Biosystems buffers were purchased from Roche Diagnostic (France ...
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bioRxiv - Microbiology 2023Quote: ... two complete EDTA-free protease inhibitor tablets (Roche) and 20 U DNase I (NEB ...
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bioRxiv - Microbiology 2020Quote: ... gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems) and sequenced on an Illumina NextSeq (High Output ...
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bioRxiv - Immunology 2021Quote: ... was used for the preparation of sequencing libraries corresponding to surface marker and cytokine while additional part (5 µl) was used for the construction of sequencing library of sample hashtags using KAPA HiFi Master Mix (Roche). The 2nd-round P7/read 2-incorporated PCR primers used for protein library preparation were listed in Table S2 with the P5/read 1-incorporated primer the same to that used in cDNA library ...
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bioRxiv - Microbiology 2020Quote: ... The sgRNA expression cassettes were amplified from gDNA in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems). For PCR1 ...
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bioRxiv - Systems Biology 2022Quote: ... CRISPR gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems). In the first PCR step ...
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bioRxiv - Genomics 2024Quote: ... We used 10 ng of DNA from two biological replicates to generate sequencing libraries using KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... using the Library Quantification Kit (Roche Molecular Systems, Pleasanton, CA). Bisulfite and RNA sequencing reactions were performed on a NextSeq500 using a HighOutput chemistry ...
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bioRxiv - Cancer Biology 2023Quote: ... the TeloTAGGG Telomere Length Assay kit (Roche Molecular Systems, USA) was utilized ...
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bioRxiv - Biochemistry 2020Quote: ... 10mM β-mercaptoethanol) and two protease inhibitor tablets (Roche) added ...
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bioRxiv - Biochemistry 2021Quote: ... with two complete EDTA-free protease inhibitor tablets (Roche). Cells were clarified by centrifugation ...
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bioRxiv - Evolutionary Biology 2021Quote: Two-color in the fluorescence in situ hybridization experiment was performed following the instructions of DIG RNA Labeling Kit (Roche, Mannheim, Germany). When synthesizing probe ...
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bioRxiv - Neuroscience 2021Quote: ... was selected based on the levels of CAT expression which were determined in brain tissue homogenates of two-month old CAG-CAT-Prnp mice using the CAT ELISA kit (Roche, Basel, Switzerland) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Cell equivalents were calculated according to amplification of the β-globin gene (two copies per diploid cell) with commercially available materials (Control Kit DNA; Roche Diagnostics). 2-LTR circles ...
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bioRxiv - Microbiology 2023Quote: For NGS library preparation we added adapters and unique dual indices in a two-step amplification process using the KAPA HiFi HotStart Library Amplification Kit (KAPA Biosystems, KK2611) and purified samples with Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Plant Biology 2021Quote: ... and two tablets of EDTA-free protease inhibitor cocktail (Roche). Unless otherwise stated ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... The detection kit for the antibodies is the UltraView DAB detection Kit (Ventana Medical Systems Inc./ Roche Diagnostic). A counter-staining of the nuclei was used for 12 minutes by Hematoxylin ...
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bioRxiv - Molecular Biology 2019Quote: Two microliters RNA was used to generate cDNA using Transcriptor (Roche), random hexamer primer ...
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bioRxiv - Neuroscience 2024Quote: ... DIG-labeled hybrids were detected with an anti-DIG antibody from sheep conjugated with alkaline phosphatase as recommended by the manufacturer (Roche) by incubating the slice overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we used a long-range PCR kit (Expand Long Template PCR System, Roche) to amplify a long fragment of mitochondrial DNA (mtDNA ...
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bioRxiv - Molecular Biology 2022Quote: RNA was extracted using the High Pure RNA isolation kit (Roche Molecular Systems) according to manufacturer’s instructions and following the general precautions required for RNA work (39) ...