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Citations for Roche :
151 - 200 of 2114 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and minced followed by enzymatic digestion in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001) and 100 U/ml Hyaluronidase (Sigma #H3506 ...
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bioRxiv - Immunology 2023Quote: ... spleens and were minced and digested in 5 mL of Iscove’s modified Dulbecco’s media (IMDM) + 10% fetal calf serum (FCS) (cIMDM) with 250 μg/mL collagenase B (Roche) and 30 U/mL DNaseI (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Cultures were then scraped in RIPA buffer (as described above) plus complete protease inhibitor tablet (Roche; Basel, Swtizerland), PMSF (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... OPCs were removed from the tissue culture plates gently with 0.2 Wünsch unit (WU)/ml of Liberase DH (Roche) and DNase I in PGM at 37°C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... tissue culture-treated plate as described above and 50 μg/ml RNase (Cat#109142, Roche CustomBiotech, Indianapolis, IN) or 50 μg/ml DNase (04536282001 ...
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bioRxiv - Immunology 2024Quote: ... RNA was harvested by removal of culture medium and addition of 200 µl of 1 M DTT (Roche) supplement Kingfisher RNA lysis buffer (Thermofisher) ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected and digested for 30min at 37°C using RPMI media (2% FBS+ Pen/Strep, glutamine, 10mM HEPES) containing 0.5mg/mL Collagenase D (Roche). The digested fragments were passed through a 70μm cell strainer (Greiner bio-one ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cultures were centrifuged at 700 g for 3 min to remove the media and parasites were isolated from the RBC by incubating with 0.1% saponin in PBS containing protease (Roche) and phosphatase inhibitors (Sigma ...
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bioRxiv - Immunology 2020Quote: Lungs were aseptically isolated from mice and incubated at 37°C in 10% FBS RPMI media containing 1.4 mg/ml collagenase A (Roche) and 30 μg/ml DNase I for 60 min ...
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bioRxiv - Immunology 2022Quote: ... The remaining tissue was then incubated with constant shaking at 37C in RPMI media containing 0.1mg/ml collagenase/dispase (Roche) and 20µg/ml DNase (Sigma-Aldrich ...
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bioRxiv - Genetics 2020Quote: ... These pieces were then added to 3-4 ml of digestion media containing 0.1 mg/ml Liberase (Roche, 501003280), 100 U/ml DNase I (Sigma ...
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bioRxiv - Immunology 2019Quote: Tissues from donor 390c were manually diced and <5.0g was added per Miltenyi C tube with 5mL tissue dissociation media (1x Liberase TL (0.13U/mL/DNase; Roche) and Benzonase nuclease (10U/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... the tissue was minced and enzymatically digested in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001), 100 U/ml Hyaluronidase (Sigma #H3506) ...
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bioRxiv - Immunology 2022Quote: ... finely chopped and incubated for 2 hours in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... were placed in 250µL of ice-cold disassociation media (sterilized 1x PBS and 0.04% bovine serum albumin, 1.2 U/mL Dispase II (Roche 04942078001), 1 mg/mL Collagenase IV (Gibco 17104–019) ...
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bioRxiv - Cancer Biology 2023Quote: ... Bones were cut into small fragments using scissors in a dissociating media containing 10 U/ml DNase I and Collagenase P (1.2 mg/ml, 11213865001, Roche). Bones and BM were digested at 37°C for 20 min under agitation ...
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bioRxiv - Immunology 2024Quote: ... followed by tissue digestion for 1 h at 37°C with Iscov’s digestion media containing 1 mg/ml Liberase TL (Roche), and 0.5 mg/ml DNAse (Thermo ...
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bioRxiv - Bioengineering 2024Quote: ... The media was removed and 150 μL of serum free RPMI containing 0.25 mg/mL Liberase TL (Roche 054010120001) were added to the samples ...
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bioRxiv - Biochemistry 2024Quote: Homogenized lungs were 10-fold diluted starting 1:10 in infection media (DMEM containing 0.1% BSA (fraction V; Roche), 10 mM HEPES (Corning) ...
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bioRxiv - Cell Biology 2020Quote: ... L6 cells and cardiomyocytes by incubating them in their respective media containing 2% (w/v) fatty acid-free bovine serum albumin (FAF-BSA; Roche) and 0.4 mM sodium palmitate (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... and re-suspended in complete media (DMEM with 10% FBS and 1% antibiotics) supplemented with Collagenase-D (1mg/mL; Roche) and incubated at 37°C for 30 minutes with shaking to form a single-cell suspension ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in complete media (DMEM with 10% FBS and 1% antibiotics) supplemented with Collagenase-D (1 mg/mL; Roche) and incubated at 37°C for 30 min with shaking to form a single-cell suspension ...
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bioRxiv - Genetics 2020Quote: ... These pieces were then added to the 3-4 ml of digestion media containing 0.1 mg/ml liberase (Roche, 501003280), 100 U/ml DNase I (Sigma ...
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bioRxiv - Immunology 2022Quote: ... were placed in 5mL of digest media (RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche)) and homogenized in gentleMACS C tubes (Miltenyi Cat#130096334 ...
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bioRxiv - Cell Biology 2021Quote: ... Retinas from adult Long Evans rats were dissected in DMEM/F12 media supplemented with complete protease inhibitors (Roche, Indianapolis, IN) and positioned above a disc of nitrocellulose disc oriented photoreceptor-side down ...
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bioRxiv - Cancer Biology 2021Quote: ... individual primary tumors were dissected from the lungs of KL mice, mechanically dissociated, then digested for 45min in digestion media (10% FBS, pen/strep, 1mg/mLCollagenase/Dispase (Roche) in DMEM ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspension was generated by mechanically disrupting tissues through a 100µm filter into FBS followed by a Percoll gradient centrifugation (40% Percoll, GE Healthcare; FBS free media containing 0.5mg/ml DNase, Roche). Cells were then washed with complete media and analyzed by flow cytometry.
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bioRxiv - Immunology 2022Quote: ... Tissue was incubated at 37°C for 15 minutes in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Immunology 2022Quote: ... Tissue was incubated at 37°C for 30 minutes in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Immunology 2022Quote: ... tumor tissue was incubated at 37°C for 30minutes in FBS free DMEM media containing liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Physiology 2023Quote: ... and then incubated for 45 min at 37°C either in Hanks’ Balanced Salt solution (HBSS) or plain DMEM media supplemented with Liberase HD (200 μg/ml, Roche) and DNase I (40 U/ml ...
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bioRxiv - Immunology 2023Quote: ... in a plate and mechanically disrupted using the back-end of a syringe before addition of 50 μL of a digestion media (dRPMI = naRPMI supplemented with 11x collagenase D (#11088866001, Roche, Woerden ...
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bioRxiv - Biochemistry 2023Quote: ... were used to inoculate each well of a 96-well plate containing 196 μL of LB media (supplemented with ampicillin, and 200uM IPTG) The plate was covered with transparent LightCycler480 Sealing Foil (Roche), and grown for 24 h in a Biotek Synergy H1 plate reader at 42 °C with double orbital shaking ...
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bioRxiv - Immunology 2023Quote: ... minced with scissors and razor blade in the presence of 1 ml of digest media (2 mg/ml collagenase IV (Roche), 1 mg/ml hyaluronidase (Worthington) ...
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mL of the culture was then treated with either 5 µl of MeOH or LMB dissolved in 7:3 MeOH:H2O solution (Roche) at a final concentration of 50 ng/mL for 45 min before imaging.
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bioRxiv - Microbiology 2021Quote: ... xanthus culture was harvested and the pellet resuspended in lysis buffer (50 mM Tris-HCl pH7.6, Protease inhibitor cocktail (Roche)) (1 ml per 80 mg cells) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from the culture supernatant after each passage using a High Pure Viral RNA kit (Roche Diagnostics). For deep sequencing of the S1/S2 cleavage site of the viral S gene ...
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bioRxiv - Biophysics 2022Quote: ... resuspended in 20mM Tris-HCl pH 8.0 (30 ml per liter of culture) containing complete EDTA-free protease inhibitor cocktail (Roche) and lysed using a Microfluidizer M-110L (Microfluidics ...
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bioRxiv - Immunology 2020Quote: ... Parasite cultures were confirmed to be free of mycoplasma and acholeplasma using an ELISA-based Mycoplasma Detection Kit (Roche) which contains polyclonal antibodies specific for M ...
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bioRxiv - Microbiology 2020Quote: ... Culture supernatant containing retroviruses was harvested after 48 hours and premixed with DOTAP liposomal transfection reagent (Roche, Basel, Switzerland) prior to spin transfection at 2400 rpm at 32°C for 2 hours into target cells ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from BHI broth cultures using the High Pure PCR template preparation kit (Roche, Potsdam, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... cells from 100 mL exponential phase cultures resuspended in 1.5 mL lysis buffer (buffer W with added cOmplete Mini protease inhibitor cocktail, Roche) were lysed by repeated sonication (10×20 s ...
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bioRxiv - Neuroscience 2022Quote: ... All co-cultures were fixed and post-fixed (before and after incubation with primary rat anti-HA antibody (Roche), respectively ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume). The suspension was frozen and stored at −80 °C ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume) were added to the cell suspension and stirred at RT for 30 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... and estrous cycle phase were pooled in PBS and minced followed by enzymatic digestion in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001) and 100 U/ml Hyaluronidase (Sigma #H3506 ...
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bioRxiv - Immunology 2021Quote: ... was added to the PBMCs for 5 minutes before transferring drop-wise to a 10ml volume of warmed media (RPMI/20%FBS) containing 200U/ml of DNase I (Roche, Switzerland). After 10 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10 ul of purified bacmid were resuspended in 130 ul Sf-900 II media with 10 ul X-treme XP transfection reagent (Roche # 06366236001) and incubated at room temperature for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... then subject to gentle cold digestion on ice using 10% FBS-supplemented RPMI media containing 100mg/mL trypsin inhibitor from soybean (Roche, 10109886001), 10mg/mL collagenase A from clostridium histolyticum (Roche ...