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Citations for Roche :
1 - 50 of 2114 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: H9 human pluripotent stem cells were maintained in E8 media and passaged every four days onto matrigel-coated plates (Roche). ESCs ...
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bioRxiv - Immunology 2022Quote: 100 μL overnight culture media of 100.000 cells/200 μL was analyzed on the CEDEX Bio Analyzer (Roche), using the Glutamine V2 Bio or Glucose Bio test kits.
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bioRxiv - Cell Biology 2020Quote: ... culture media were collected and cells harvested on ice in Nonidet lysis buffer supplemented with protease inhibitor mixture (Roche). Culture media and cell lysates were precleared and α1AT was immunoprecipitated with a α1AT polyclonal antibody (total ...
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bioRxiv - Immunology 2023Quote: ... The final sodium concentrations in the cell culture media were confirmed by potentiometry with a Cobas 8000 analyzer (Roche). T cells were stimulated with plate-bound CD3 (1 μg/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... Upon addition of 1ml of culture media containing (10µg/ml) DNaseI (Roche, 11284932001), samples were spun 15’ 500g at RT ...
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bioRxiv - Biochemistry 2020Quote: ... Ganglia were mechanically disrupted with a pipette tip until obtaining a single cell suspension in culture media supplemented with DNAse I grade II at final concentration 3mg/ml (Roche, Cat# 104159), which was centrifuged at 1300 rpm (302 rcf ...
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bioRxiv - Developmental Biology 2021Quote: Testosterone concentrations were measured in culture media using the Roche e411 analyzer (Roche Diagnostics, USA) with the Elecsys Testosterone II enzyme-linked immunosorbent assay ...
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bioRxiv - Immunology 2020Quote: ... diluted 2-fold with PBMC culture media and supplemented with 10% WST-1 reagent (Roche) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and digested in 2.5 ml of culture media containing 1 mg/mL of Collagenase IV (Roche) and 50 unit/mL DNase (Sigma ...
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bioRxiv - Neuroscience 2023Quote: Culture media collected from the treatments was supplemented with a 10× concentrated solution of PI cocktail (Roche) at a 9:1 conditioned:fresh media ratio (i.e. ...
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bioRxiv - Immunology 2021Quote: ... Culture media was supplemented with 500 U/mL human IL-2 (Tecin from Roche, kindly provided by the NIH) starting on culture day 2 ...
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bioRxiv - Immunology 2023Quote: ... Single cells suspension were generated in media containing Liberase (Roche) and DNase I (Roche ...
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bioRxiv - Neuroscience 2021Quote: LDH release in the culture media from primary hippocampal neurons was measured following manufacturer instructions (Cytotoxicity Detection KitPlus –LDH, #04 744 926 001, Roche).
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bioRxiv - Cell Biology 2021Quote: Human stem cells and neurons lysed with RIPA lysis buffer supplemented with 5mM EDTA and protease inhibitor (Roche), for 5 minutes at room temperature and 10 minutes on ice ...
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bioRxiv - Developmental Biology 2021Quote: On day 0 (start of differentiation) human pluripotent stem cells were treated with 1mg/ml Collagenase B (Roche) for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: Human induced pluripotent stem cell (iPSC) line KYOU-DXR0109B (ATCC) was routinely cultured on growth-factor reduced Matrigel (Roche) in mTeSR1 culture medium (StemCell Technologies ...
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bioRxiv - Neuroscience 2024Quote: Cell cultures and sciatic nerves were homogenized in TriPure Reagent (Roche) using Precellys Lysing Kit CK14 and homogenizer (8000 rpm ...
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bioRxiv - Cell Biology 2021Quote: Human pluripotent stem cells (hPSCs) were maintained in E8 medium and passaged every 4 days onto matrigel-coated plates (Roche). The following hPSC lines were used in the study ...
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bioRxiv - Biophysics 2020Quote: ... cells were grown in media containing 0.1 mg/mL Hygromycin (H3274, Roche).
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bioRxiv - Bioengineering 2021Quote: ... 700 µl of T cell media + 30 U/ml rhIL-2 (Roche) was added to each well ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were resuspended in pre-warmed specific media containing DNAse (Pulmozyme, Roche). After 20 h cells at 37ºC and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... Culture density was measured with a CASY model TT cell counter (Roche Diagnostics) with a 60 µm capillary and exclusion of particles with a pseudo diameter below 2.0 µm.
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bioRxiv - Immunology 2020Quote: ... The cells were plated in pMCPECs media on 50ug/ml laminin (Roche, Mannheim, Germany) coated inverted filters of 5μm pore size and 6mm diameter (Corning ...
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bioRxiv - Epidemiology 2019Quote: ... the specimens were subjected to MTB culture (Roche fixed culture test), bacterial species identification and drug sensitivity testing (Ratio methods ...
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bioRxiv - Biochemistry 2020Quote: ... MR-Fc was purified from cell culture supernatant using Protein A-agarose beads (Roche). For ELISA experiments ...
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bioRxiv - Microbiology 2021Quote: ... Fab was purified from cell culture supernatant by cOmplete His-Tag Purification Resin (Roche).
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bioRxiv - Microbiology 2020Quote: ... Cobas PCR Media (Roche); Aptima Specimen Transport Medium (Hologic) ...
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bioRxiv - Systems Biology 2021Quote: ... cell culture counts were accessed in the count plate on an automated cell counter (Cedex, by Roche, Basel, Switzerland). As there was little or no growth lag after splitting the culture ...
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bioRxiv - Biophysics 2019Quote: ... 4 million cells were seeded in a 10 cm cell culture dish for transfection using X-tremegene 9 (Roche) and Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Cells were cultured in complete media with 30 IU/mL IL-2 (Roche, 202-IL). Media containing IL-2 was replenished daily for 10 days ...
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bioRxiv - Cancer Biology 2019Quote: ... media was discarded and cells were incubated with 10 μL of WST-1 reagent (Roche) and 90 μL of growth medium for 4 hours ...
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bioRxiv - Immunology 2022Quote: ... Cells were digested in RPMI-1640 media containing 20 μg/mL DNase (Roche, Basel, Switzerland) and 1 mg/mL collagenase IV (StemCell Technologies ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed in 10 mL staining media containing DNase I (Roche, Basel, Switzerland) and then resuspended in 100 μL of staining media containing 4 μg/mL of biotinylated RAVV GPΔMuc conjugated to streptavidin PE and 4 μg/mL of biotinylated RAVV GPΔMuc conjugated to streptavidin APC and incubated for 20 minutes [45] ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche). Quality control of A33-His was performed by SDS-PAGE and size exclusion chromatography (Superdex75 10/300GL ...
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bioRxiv - Neuroscience 2021Quote: ... Detection and quantification of apoptosis in cell cultures was determined using a In Situ Cell Death Detection Kit (Roche, 11684795910) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... cultures were treated according to the protocol of In Situ Cell Death Detection Kit (Roche). Before observation ...
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bioRxiv - Cancer Biology 2022Quote: ... media was exchanged with organoid media supplemented with 1mg/ml G418 (Roche, G418-RO) to begin selection.
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bioRxiv - Neuroscience 2024Quote: ... mechanically dissociated in a single cell suspension in media containing DNase I (Roche, 100 ug/ml) using repeated pipetting and concentrated by centrifugation (3 min at 800 X g) ...
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bioRxiv - Molecular Biology 2022Quote: ... cell culture supernatants were harvested and proteins were purified from the supernatants using tandem Ni2+ (Roche) and Strep-Tactin (IBA ...
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bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in AYE media containing a complete mini EDTA-free protease inhibitor cocktail tablet (Roche) and adjusted to an OD600 of 20 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in complete media plus 10 IU/mL recombinant human IL-2 (rHIL-2, Roche), and seeded in fresh media at 0.5×106 cells/mL every 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... the LDH release in cell culture supernatant from infected cells was measured at 24 h and 96 hpi by using the colorimetric kit (Roche, Mannheim, Germany) and following the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: Culture density was measured with a haemocytometer (BSFs) or a CASY model TT cell counter (Roche Diagnostics) with a 60 µm capillary and exclusion of particles with a pseudo diameter below 2.0 µm (PCFs).
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bioRxiv - Microbiology 2020Quote: ... GISAID: EPI_ISL_413570) obtained from cell culture using the MagNA Pure LC Total Nucleic Acid Isolation Kit (Roche). We determined the slope by linear regression in GraphPad Prism and defined the required levels for PCR efficiency (E ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell culture suspension was supplemented with DNase I and EDTA-free protease inhibitor cocktail tablet (Roche) and lysed by passing through an EmulsiFlex-05 cell disruptor (Avestin ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell culture suspension was supplemented with DNase I and EDTA-free protease inhibitor cocktail tablet (Roche) and cells were lysed by passing through an EmulsiFlex-05 cell disruptor (Avestin ...
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bioRxiv - Cancer Biology 2020Quote: ... All the cells in culture were negative on periodically testing for mycoplasma using Mycoplasma Detection Kit (Roche). All the human and mouse cells ...
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bioRxiv - Biochemistry 2021Quote: ... the cell culture suspension was supplemented with DNase I and EDTA-free protease inhibitor cocktail tablet (Roche) and cells were lysed by passing through an EmulsiFlex-05 cell disruptor (Avestin ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 µL of cell culture supernatant was collected and the Cytotoxicity Detection Kit (LDH) (Roche, Indianapolis, IN) was then utilized to detect LDH levels in the culture supernatant ...
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bioRxiv - Immunology 2021Quote: The presence of lactate dehydrogenase (LDH) in cell culture supernatants was measured using the Cytotoxicity Detection kit (Roche), following manufacturer’s instructions ...