Labshake search
Citations for Roche :
301 - 350 of 6336 citations for SpectraDye Antibody Labeling Kit 350 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
-
bioRxiv - Pathology 2020Quote: ... the sequence was labeled with digoxigenin using the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Diagnostics Deutschland GmbH, Germany). After dewaxing in water ...
-
Dissemination of linezolid-resistance through sex pheromone plasmid transfer in Enterococcs faecalisbioRxiv - Microbiology 2019Quote: ... blots were hybridized with digoxigenin (DIG)-labeled optrA-specific probe using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche Applied Sciences, Germany) following the manufacturer’s instructions.
-
bioRxiv - Genetics 2022Quote: ... Sense and antisense digoxigenin (DIG)-labeled probes were synthesized from the purified PCR product using DIG RNA Labeling Kit (Roche, cat. no.11175025910). All primer sequences were listed in Supplementary Information (Supplementary Table 3).
-
bioRxiv - Genetics 2022Quote: Southern blot analysis was performed to ensure that the foreign gene Bar was present in randomly excised segments of the T2 transformed soybean genome by following the protocol from the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche, Indianapolis, IN, USA). DNA was extracted from the plants with positive PCR results ...
-
bioRxiv - Developmental Biology 2022Quote: ... Digoxygenin-labeled sense and antisense probes were synthesized from the linearized plasmids using the DIG RNA Labeling Kit (SP6/T7) (Roche/MilliporeSigma, Burlington, MA). Whole-mount ISH was performed as previously described (Yan et al. ...
-
bioRxiv - Immunology 2022Quote: Tumor tissue sections were subjected to the terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling (TUNEL) assay using the In Situ Cell Death Detection Kit (Roche Diagnostics, Indianapolis, IN) according to the manufacturer’s protocol ...
-
bioRxiv - Zoology 2023Quote: ... Digoxigenin-labeled antisense and sense RNA probes were produced from cloned PCR fragments using a digoxigenin RNA labeling kit (SP6/T7; Roche Diagnostics, Basal, Switzerland). Labeling was performed as previously described (Ukena et al. ...
-
bioRxiv - Developmental Biology 2023Quote: Probes for whole mount ISH and FISH were prepared with RT-PCR using primers listed in Table S7 followed by ligation into pGEM-T Easy vectors and transcribed using a DIG RNA labeling kit (Roche, Cat no. 11175025910), some probes were made using flourescein labeling mix (Roche ...
-
bioRxiv - Neuroscience 2024Quote: ... brain sections were processed for DNA strand breaks using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) from Fluorescence In Situ Cell Death Detection kit (Roche Diagnostic, Indianapolis, IN) according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2021Quote: ... DIG-labeled RNA probes were then synthesized (DIG RNA Labeling Mix, Roche) following the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2020Quote: ... Antisense cRNA transcripts were synthesized with DIG labeling mix (Roche Life Science) and T3 or SP6 RNA polymerase (New England Biolabs Inc.) ...
-
bioRxiv - Neuroscience 2022Quote: ... from the linearized plasmids with DIG or FITC RNA labeling mix (Roche), respectively.
-
bioRxiv - Neuroscience 2020Quote: ... We synthesized the probe using the DIG RNA Labeling Mix (Roche, 11277073910).
-
bioRxiv - Developmental Biology 2019Quote: The RNA probe was synthesized using the Roche DIG labeling mix (Roche) and detected with an anti-DIG antibody coupled to alkaline phosphatase (1:5000 ...
-
bioRxiv - Genetics 2020Quote: ... Probes were generated with the PCR DIG labeling Mix (Roche, Mannheim, Germany) following the manufacturer’s instructions and chemiluminescent signals were detected using the GelDocTM XR+ system (Bio-Rad ...
-
bioRxiv - Neuroscience 2021Quote: ... Premixed RNA labeling nucleotide mixes containing digoxigenin-labeled UTP (Roche Molecular Biochemicals) were used to generate RNA riboprobes ...
-
bioRxiv - Developmental Biology 2020Quote: ... Transcription of probes was done using the DIG RNA Labeling Mix (Roche). All protocols were used according to the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2023Quote: ... some probes were made using flourescein labeling mix (Roche, Cat. No 11685619910). Plasmids for Pax37A and Pax37B probes was kindly provided by Dr ...
-
bioRxiv - Developmental Biology 2024Quote: ... DIG-labeled probes were prepared using a DIG RNA labeling mixture (Roche) and the T7 (antisense probe ...
-
bioRxiv - Genomics 2024Quote: ... in vitro synthesized from a linearized plasmid using RNA Labeling Mix (Roche) and T3 RNA polymerase (Roche) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin-labeled riboprobes were transcribed using the DIG-RNA Labeling Mix (Roche). Embryonic heads (E11.5–E13.5 ...
-
bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS before 350 µl of external lysis buffer (Roche, Penzberg, Germany) was added to the cells ...
-
bioRxiv - Developmental Biology 2020Quote: Digoxigenin (DIG)-labeled and Fluorescein (Flu)-labeled in-situ probes were synthesized using in vitro transcription with Flu/DIG RNA Labeling kits respectively (Roche,Cat#11175025910, Cat#11685619910). RNA in-situ hybridization was conducted according to the published protocol 85 ...
-
bioRxiv - Microbiology 2020Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was conducted using an in-situ cell death detection kit (Roche, Indianapolis, Indiana, United States) as per the manufacturer’s instructions [31,34] ...
-
bioRxiv - Developmental Biology 2020Quote: ... Riboprobes were synthesized from the plasmids by in vitro transcription using either T7 or Sp6 polymerase and a digoxigenin (DIG) labeling kit (Roche Applied Science, Indianapolis, IN). The primer sequences used for riboprobe preparation are given in Supplemental Table 1.
-
bioRxiv - Genomics 2021Quote: ... Sense and antisense digoxigenin-labelled RNA probes were generated by in vitro transcription using the DIG-RNA Labeling Kit (SP6/T7; Roche Applied Sciences, QC, Canada). Labeled RNA probes were quantified by dot blot analysis.
-
bioRxiv - Neuroscience 2023Quote: ... brain sections were processed for DNA strand breaks using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) from Fluorescence In Situ Cell Death Detection kit (Roche Diagnostic, Indianapolis, IN, US) according to the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2023Quote: ... The PCR primer for generating the probe was 5’-GGTTCTGTGATTGAGTGTTTGGATCTCCCTGCG-3’ with a single-end CY3 tag generated using the DIG RNA Labeling Kit (Roche Applied Science, Penzberg, Germany) based on the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... or Sp6 RNA polymerase (Table S3) and the Dig-RNA labeling mix (Roche). The probes were purified using the RNA easy mini kit ...
-
bioRxiv - Developmental Biology 2019Quote: ... Antisense RNA probes were synthesized using digoxigenin or fluorescein RNA labeling mix (Roche) using standard methods ...
-
bioRxiv - Molecular Biology 2021Quote: ... Both probes were labeled by random hexamer labeling with High Prime (Roche, 11585592001) and [α-32P]-dCTP ...
-
bioRxiv - Neuroscience 2021Quote: ... Probes were digoxigenin-labeled using the DIG RNA Labeling Mix purchased from Roche.
-
bioRxiv - Molecular Biology 2021Quote: Antisense RNA probes labeled with digoxigenin (DIG RNA Labeling Mix 10 × conc, Roche) and biotin (Biotin RNA Labeling Mix 10 × conc ...
-
bioRxiv - Neuroscience 2020Quote: ... Nuclear labeling was completed by incubating slices with DAPI (0.1 μg/mL, Roche) at room temperature for 5 min after incubation with secondary antibodies ...
-
bioRxiv - Neuroscience 2021Quote: ... Nuclear labeling was completed by incubating slices with DAPI (0.1 μg/mL, Roche) at RT for 5 min after incubation with secondary antibodies ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... and digoxigenin-UTP (DIG RNA labeling mix; Roche Applied Science, Indianapolis, IN, USA). The riboprobe was purified with EtOH precipitation and mini Quick Spin Columns (Roche ...
-
bioRxiv - Developmental Biology 2020Quote: ... Probes were synthesized with T7 polymerase and DIG labeling mix (Roche 10881767001, 11277073910).
-
bioRxiv - Evolutionary Biology 2022Quote: ... Digoxigenin-labeled probes were generated using a 10X Dig labeling mix (Roche Diagnostics) and T7 RNA polymerase (Promega) ...
-
bioRxiv - Molecular Biology 2022Quote: Antisense RNA probes labeled with digoxigenin (DIG RNA Labeling Mix 10 × conc, Roche) or biotin (Biotin RNA Labeling Mix 10 × conc ...
-
bioRxiv - Cell Biology 2022Quote: ... in presence of Digoxygenin-labeled nucleotides (DIG RNA Labeling Mix, Roche, Bâle, Switzerland).
-
bioRxiv - Immunology 2023Quote: Apoptosis was analyzed with terminal deoxynuclotidyltransferase-mediated dUTP nick-end labeling (TUNEL; Roche) following the manufacturer’s protocol ...
-
bioRxiv - Physiology 2024Quote: ... with fluorescein using T7 RNA polymerase and Fluorescein RNA Labeling Mix (Roche Diagnostics) and with DIG as described above.
-
bioRxiv - Cell Biology 2020Quote: ... Cells were pelleted then washed once with 250 uL TAP extraction buffer (40 mM HEPES pH 7.5, 10% Glycerol, 350 mM NaCl, 0.1% Tween-20, phosphatase inhibitor cocktail and proteinase inhibitor cocktail from Roche). Cell pellets were resuspended in 180 uL modified 2X SDS buffer then boiled at 100 °C for 4-5 minutes ...
-
bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR (qRT-PCR) analysis was performed using LightCycler Quick System 350S (Roche Applied Science, Penzberg, Germany) with Thunderbird SYBR qPCR Mix (Toyobo) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Nuclei were washed once in 1 mL Wash350 (20 mM HEPES pH 7.5, 350 mM NaCl, 10 mM NaButyrate, 0.025% Digitonin, protease inhibitor cocktail (Roche), 0.5 mM Sper-midine ...
-
bioRxiv - Cell Biology 2020Quote: ... biotin-labeled RNAs were in vitro transcribed with the Biotin RNA Labeling Mix (Roche) and T7/T3 RNA in vitro transcription kit (Ambion ...
-
bioRxiv - Immunology 2019Quote: ... DNA probes were generated for each strain by labeling with digoxigenin (Roche, Basel, Switzerland) 28 ...
-
bioRxiv - Molecular Biology 2022Quote: Probes were labeled according to the Nick Translation-based DNA Probe Labeling method (Roche). Tak-1 and Tak-2 genomic DNA was extracted by CTAB method (Murray & Thompson ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: c-fos DIG antisense riboprobe was in vitro transcribed (DIG RNA Labeling Mix, Roche) from an EcoRI fragment of c-fos cDNA (Dr Inder Verma ...