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Citations for Roche :
551 - 600 of 1607 citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... pastoris were removed by incubation with 5 mU of EndoglycosidaseH (Roche) in 50 mM sodium citrate buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... 5–10 mM NaF) containing a protease inhibitor cocktail (Roche, 04693132001) and the protein lysis buffers were incubated on ice for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... PCR conditions were as follows: 5 μl Kapa Enhancer (Kapa Biosystems), 5 μl Kapa Buffer A ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 2) 5 10^6 copies of bacteriophage MS2 RNA (Roche) were spiked in per isolation ...
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bioRxiv - Neuroscience 2019Quote: ... 5 mM DTT and protease inhibitors (#04 693 159 001 Roche). After a squeezing step ...
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bioRxiv - Genomics 2019Quote: ... 0.08U KAPA2G Robust HotStart DNA Polymerase (5 U/μL, Roche KK5517). PCR reactions were performed using a thermocycler with the following conditions ...
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bioRxiv - Neuroscience 2020Quote: ... The PVDF membranes were blocked in 5% Western Blocking Reagent (Roche) then probed with primary and secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... 5 μl FastStart Universal SYBR Green Master mix (Roche, Basel, Switzerland) and 3 μl MilliQ ...
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bioRxiv - Neuroscience 2020Quote: ... 5 mM magnesium acetate) supplemented with protease/phosphatase inhibitor cocktail (Roche) and RNasin Plus (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... 5% (w/v) glycerol] supplemented with complete protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail (Sigma ...
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bioRxiv - Immunology 2022Quote: ... samples were incubated in 5 mM 1,4-dithiothreitol (Roche, Basel, Switzerland) for 20 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM Benzamidine and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche)) was added and mixed by pipetting ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μl of 10X 5-Bromo-2’-deoxyuridine (BrdU) (Roche, Germany) per well was added ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM β-mercaptoethanol and protease inhibitors (cOmplete, Roche, Basel, Switzerland) and lysed mechanically using EmulsiFlex (Avestin ...
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bioRxiv - Microbiology 2023Quote: ... 5 mM β-ME) with complete EDTA-free protease inhibitor (Roche). The cells were lysed by sonication (Vibra-Cell™ VCX 130 ...
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bioRxiv - Neuroscience 2023Quote: ... and BCIP (5-bromo-4-chloro-3-indolyl-phosphate, Roche 11383221001). Larvae were mounted in 100% glycerol under coverslips and bright-field images captured using a Leica DFC500 camera mounted on a Zeiss Axioskop
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bioRxiv - Plant Biology 2023Quote: ... 5 mM Benzamidine and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche)) and incubated on ice for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM Na-molybdate and EDTA-free protease inhibitor cocktail (Roche). Protein concentration was measured using the Bradford method (Bio-Rad # 500-0006) ...
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bioRxiv - Cell Biology 2022Quote: ... Aliquots were incubated with anti-GFP (5 µg, Roche, # 11814460001, Germany) for GFP or HA11(5ug ...
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bioRxiv - Biophysics 2023Quote: ... 5 % glycerol and cOmplete™ EDTA-free protease inhibitor cocktail (Roche).
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bioRxiv - Cell Biology 2022Quote: ... 5 mM Na-molybdate and EDTA-free protease inhibitor cocktail (Roche). Protein concentration was measured using the Bradford method (Bio-Rad # 500-0006) ...
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bioRxiv - Cell Biology 2023Quote: ... and 5% IGEPAL CA-630 supplemented with cOmplete protease inhibitors (Roche), and incubated on ice for 15 mins ...
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bioRxiv - Biochemistry 2023Quote: ... 5% glycerol supplemented with DNase1 and 1xEDTA-free protease inhibitors (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: ... 5 units/mL Benzonase and an EDTA-free protease inhibitor (Roche). After sonication ...
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bioRxiv - Developmental Biology 2024Quote: ... 5’-AGCGTTCACATCATATGGCA-3’) using Taq DNA polymerase (Roche, Cat. No. 11146165001). Fragments of foxl2l and id1 were cloned into pGEMT-easy plasmid by TA cloning while nanos2 fragment was cloned into pCS2+ plasmid by BamHI and KpnI ...
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bioRxiv - Neuroscience 2024Quote: ... 5 µM MG132) and a protease inhibitor cocktail (Roche, Basel, Switzerland), as previously described72 ...
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bioRxiv - Bioengineering 2024Quote: ... with EDTA (invitrogen, #15575-038) (5 mM) and pronase (Roche, #10165921001) (1 mg/mL) ...
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bioRxiv - Neuroscience 2024Quote: ... and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP; 11383221001, Roche) was performed in alkaline phosphatase reaction buffer [100 mM Tris pH 9.5 ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM DTT) supplemented with EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication for ten cycles of 30 pulses of sonication and 30 s breaks using a Hielscher UP200st set to pulse=70% ...
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bioRxiv - Molecular Biology 2021Quote: ... and 20 mM HEPES) supplied with 30 U/ml of human recombinant IL-2 (rIL-2, Roche). Four domains of SUPT16H ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in complete media plus 10 IU/mL recombinant human IL-2 (rHIL-2, Roche), and seeded in fresh media at 0.5×106 cells/mL every 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Vector DNA was transiently transfected into human embryonic kidney (HEK) cells using Fugene-6 (Roche Molecular Biochemicals) transfection reagent ...
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bioRxiv - Immunology 2021Quote: ... Antibody protein treatments (anti-SARS-CoV-2 mAbs or human IgG1 isotype control Trastuzumab/Herceptin® (Roche)) were initiated 24 hours post infection by intraperitoneal injection ...
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bioRxiv - Immunology 2021Quote: Total RNA was retrotranscribed and cDNA was quantified using the Universal Human Probe Roche library (Roche Diagnostics). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... and goat anti-human AF647 (Stratech 109-606-088- JIR)) DNA was then stained with DAPI (Roche) and coverslips mounted with Vectashield (Vector Laboratories - Vector H-1000).
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bioRxiv - Immunology 2024Quote: Mice or Human CD4 T cells were lysed using RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Pierce) ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...
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bioRxiv - Molecular Biology 2019Quote: ... and were then stained with the mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) and the FITC-tagged secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: Human stem cells and neurons lysed with RIPA lysis buffer supplemented with 5mM EDTA and protease inhibitor (Roche), for 5 minutes at room temperature and 10 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... freshly resected human samples were cut into small fragments and digested with 0.1 mg/ml Liberase TL (Roche) and 0.1 mg/ml DNase (Roche ...
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bioRxiv - Developmental Biology 2021Quote: On day 0 (start of differentiation) human pluripotent stem cells were treated with 1mg/ml Collagenase B (Roche) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... human vimentin immunohistochemical staining of the paraffin sections was performed by using the Ventana Discovery XT instrument (Roche) with Ventana DAB Map detection Kit (760-124 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... lumbar vertebrae 1 – 5 were incubated in 2% Collagenase P (Roche, Switzerland) for 30 minutes at 30° C ...
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bioRxiv - Genomics 2022Quote: ... 5 μl of 2X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602).
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bioRxiv - Genomics 2020Quote: ... Nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche) were used for the colorimetric detection of alkaline phosphatase activity ...
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bioRxiv - Biochemistry 2020Quote: ... 5% Glycerol and EDTA free protease inhibitor cocktail tablet/50ml (Roche, UK)] ...
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bioRxiv - Cell Biology 2021Quote: ... 5% glycerol ()AppliChem #A2926) and phosphatase inhibitors (PhosSTOP™, Roche, Indianapolis, IN), 2 mM sodium orthovanadate ...
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bioRxiv - Biochemistry 2021Quote: ... 5% (v/v) glycerol with complete EDTA-free protease inhibitor cocktail (Roche) and 10 µg/ml DNAseI (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM imidazole) supplemented with complete EDTA-free protease inhibitor tablets (Roche) and disrupted by sonication ...
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bioRxiv - Biochemistry 2021Quote: ... 5 %(v/v) glycerol and cOmplete EDTA-free protease inhibitor tablets (Roche).