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Citations for Roche :
501 - 550 of 1607 citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and 5 mM EDTA with cOmplete mini protease inhibitors (Roche) and phosphatase inhibitor cocktails 2 and 3 (Sigma) ...
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bioRxiv - Physiology 2023Quote: ... 5 mM EDTA: pH = 7.4) supplemented with protease inhibitors (Roche) and PhosStop tablets (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... 5%β-mercaptoethanol) supplemented with Complete Protease Inhibitor Cocktail (Roche) and PhosSTOP Phosphatase Inhibitor Cocktail (Roche) ...
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bioRxiv - Genomics 2023Quote: ... The KAPA Library Quantification DNA Standards #1–5 (Roche #07960387001) were used for the standard curve ...
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bioRxiv - Biochemistry 2023Quote: ... 5% glycerol supplemented with EDTA-free Complete Protease Inhibitor (Roche) and Salt-Active Nuclease (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% glycerol and a protease and phosphatase cocktail inhibitor (Roche). Lysates were rotated at 4°C for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% glycerol and a protease and phosphatase cocktail inhibitor (Roche). Lysates were vortexed to homogenize and incubated on ice for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 5% glycerol) with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). After lysate centrifugation at 48.384g for 45 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... #11383213001)/BCIP (5-bromo-4-chloro-3-indolyl phosphate, Roche, #11383221001) color development substrate via AP activity ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mg/ml NEM and protease inhibitors (Roche, Mannheim, Germany)) ...
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bioRxiv - Plant Biology 2023Quote: ... and 5 µL of Master mix SYBR Green I (Roche). Reactions were run in triplicates using a LightCycler® 480 II (Roche ...
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bioRxiv - Microbiology 2023Quote: ... with 5 nanomoles of oligo [dT] (average length 18nt) (Roche) in TE (10mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM 2-mercaptoethanol (BME) and protease inhibitor cocktail (Roche) using a combination of dounce homogenization and sonication ...
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bioRxiv - Developmental Biology 2023Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate solution (Roche). Images were captured using a BX-50 microscope (Olympus Corp. ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 mM MgCl2 and 1x cOmplete protease inhibitor cocktail (Roche) and lysed using a Microfluidizer ...
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bioRxiv - Biophysics 2023Quote: ... 5 mM MgCl2 and cOmplete protease inhibitor EDTA-free (Roche), pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... 5 mM MgCl2) completed with complete protease inhibitor cocktail (Roche) and disrupted with a Bioruptor Sonicator by sonication (2 min ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked in TBS containing 5% BSA (Roche, 10735086001) for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... with glass coverslip (coated with 5 μg/cm2 fibronectin (Roche) for an hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% beta-mercaptoethanol and protease and phosphatase inhibitors (Roche Complete Mini protease inhibitor ...
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bioRxiv - Neuroscience 2023Quote: ... 5% glycerol plus a protease inhibitor cocktail (PIC) (Roche, 046693132001). One % Triton X-100 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: The gDNA libraries were prepared using a SeqCap EZ Human Exome Library v2.0 (Roche, Basel, Switzerland). Sequencing was performed with 100-bp paired-end reads by the HiSeq2000 sequencer (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 ng of labeled probe was mixed with 10 μg of human Cot-1 DNA (Roche) and 10 μg each of salmon sperm DNA and E ...
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bioRxiv - Neuroscience 2022Quote: ... Primers were designed using the Universal Probe Library (UPL) Probefinder software for human (Roche, version 2.53) or using previously published sequences and adjusted to be intron-spanning ...
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bioRxiv - Immunology 2019Quote: For the in vitro cell stimulation and maintenance reagents were as follows: human IL-2 (Roche); IL-21 (PeproTech) ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used for the immunostaining include human ERα rabbit monoclonal antibody (Roche Diagnostics, 790-4325), human PR rabbit monoclonal antibody (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... Regions to sequence were selected with the SeqCap EZ Human Exome Library v3.0 (Roche Applied Science) according to the manufacturer’s instructions and underwent 2 × 151 base-pair sequencing on Hiseq 4000 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... were transfected into the human 293FT cell line using X-tremeGENE 9 DNA transfection reagent (Roche). After 48 hours ...
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bioRxiv - Immunology 2023Quote: ... the medium was supplemented with 30 IU/mL recombinant human interleukin 2 (IL-2) (Proleukin, Roche). Electroporated T cells were kept in culture ON and then used directly or frozen.
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bioRxiv - Developmental Biology 2021Quote: Immunostaining was performed after FISH development by re-blocking planarians in Blocking Solution (5% heat inactivated horse serum, 5% Roche Western Blocking Buffer in TNTx) for 2 hours at room temperature ...
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bioRxiv - Genetics 2020Quote: ... A LYS2 probe was amplified and labeled with Digoxigenin (DIG)-dUTP using the primers 5’-TGAAGCCTTCCCAGAGAGAA and 5’-GCCAAGGAAAAATGTCTACCA (Roche PCR DIG Probe Synthesis Kit). The DIG probe was hybridized to the membrane (at 44°C ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG; or anti-GFP, 1:1000, Roche #11814460001 in 3% BSA/TBS-T) at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM EDTA) supplemented with phosphatase inhibitors (5 mM ß-glycerophosphate, 5 mM NaF, 1 mM Na3VO4) and protease inhibitors (Roche cOmplete ULTRA Tablets, EDTA-free). The lysates were sonicated on ice (4x 10s bursts ...
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bioRxiv - Biophysics 2021Quote: ... 5 μL FastStart SYBR Green Master (Roche Diagnostics, Indianapolis, IN USAl), 0.4 μL qRT-PCR primers (Table S2) ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.3 μl of 5 U/μl FastStart Taq DNA polymerase (Roche), 0.4 μl of 100 μM “up” or “down” barcode primer pair ...
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bioRxiv - Microbiology 2019Quote: ... or 5 μl RNA molecular weight marker I DIG-labelled (Roche) were treated in the same way as the RNA samples to allow the detected transcript length to be estimated ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% glycerol: pH 7.4) containing protease and phosphatase inhibitor cocktails (Roche) for 30 min at 4°C with intermittent mixing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 % DMSO and 12.5 μL of KAPA HiFi HotStart ReadyMix (Roche). The parameters for PCR were 95 °C for 5 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 % DMSO and 12.5 μL of KAPA HiFi HotStart ReadyMix (Roche). The parameters for PCR were 95 °C for 5 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 % DMSO and 12.5 μL of KAPA HiFi HotStart ReadyMix (Roche). The primers used to amplify the library were P1 (5’-GGGGAAGCTTGCCGTAAG-3’ ...
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bioRxiv - Neuroscience 2021Quote: ... and .5 mM spermidine along with phosphatase and protease inhibitors (Roche). All steps were performed on ice ...
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bioRxiv - Neuroscience 2021Quote: ... and 5’-GCTTGAGGTAGC CCTGTTGTCACC-3’ using KAPA HiFi Hotstart polymerase (Roche:KK2602). This PCR reaction produced a 185 bp wild type band and a 750 bp knockout band in heterozygous animals ...
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bioRxiv - Bioengineering 2021Quote: ... 5 μl 2500 U/ml DNase I (04716728001 Roche, Sigma Aldrich) was added to the single-cell suspension 10 min before the end of the digestion ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 mM CaCl2 and 15 units of S7 micrococcal nuclease (Roche). Lysates were sonicated for 10 seconds at low power followed by incubation on ice for 30 minutes and clarification by centrifugation at 13,000 x g for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 5% glycerol) containing 1× complete EDTA-free protease inhibitor cocktail (Roche). Insoluble debris was removed by centrifugation at 15000 rpm for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... ATP 5 mM) supplemented with proteases inhibitors (cOmplete EDTA-free, Roche). Cells were homogenized in a ball homogenizer with 10 μm clearance ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% (w/v) glycerol) with protease inhibitor tablet (EDTA-free, Roche), and lysed by sonication ...
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bioRxiv - Developmental Biology 2022Quote: ... and 0.035 mg/ml 5-bromo-4-chloro-3-indolyphosphatase (Roche). Images were acquired by the Leica MZ10F microscope with the DS-Ri1 camera.
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bioRxiv - Cell Biology 2021Quote: ... and digested with collagenase D (5 mg/mL, #11088882001, Roche, Germany) and dispase (2 mg/mL ...