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Citations for Roche :
401 - 450 of 1949 citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Thee injections of either an anti-GluA2 antibody (clone 15F1, gift from E. Gouaux) or a monoclonal anti-GFP IgG1-K (Roche, 11814460001) were targeted to the L2/3 of S1 (−0.1 to 0.3 mm dorsoventral) ...
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bioRxiv - Microbiology 2022Quote: ... with the IDT E Assay First Line Screening primers and probe (Cat # 10006804) on a LightCycler 96 System (Roche, Cat# 05815916001).
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bioRxiv - Genetics 2019Quote: ... and RIPA double-detergent buffer (2% deoxycholate, 2% NP40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with a protease-inhibitor cocktail (Roche). Cells were subsequently incubated on ice for 30 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... 10 mM MgCl2, 2 mM DTT, 2 µM leupeptin, 2 mM PMSF, 250 mM sucrose, and 1× PhosSTOP phosphatase inhibitors [Roche]). The homogenate was centrifuged for 15 min at 10,000 × g at 4°C ...
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bioRxiv - Genetics 2023Quote: ... and RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche), as described previously (Kemaladewi et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Protein was equally loaded on SDS-PAGE for immunoblotting and the HA tag was detected using an anti-HA antibody (Roche, #11867423001 www.sigmaaldrich.com) and Femto chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP) and cOmplete protease inhibitors (Roche). Cells were lysed by sonication and cell debris pelleted at 25,000 rpm for 40 min ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mM Mg(OAc)2 supplemented with phosphatase and protease inhibitors (Roche) (adapted from (29)) ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-GFP (1:1000, 0.4 µg/ml; Roche).
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-GFP (7.1+13.1, Roche, 11814460001; 1:1000), rabbit anti-Caveolin (BD Biosciences 610059 ...
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bioRxiv - Biochemistry 2019Quote: ... mouse-anti-GFP (1:1000) (Roche Cat# 11814460001, RRID:AB_390913), and rabbit-anti-Hxk1 (1:3000 ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with recombinant DNASE I (1000 U) (Roche, 0453628001), cOmplete protease EDTA-free inhibitor cocktail (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:1000 for anti-HA (rat mAb, 3F10, Roche), anti-flag (mouse mAb ...
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bioRxiv - Developmental Biology 2020Quote: ... or for FISH (Roche anti-DIG-POD 1:1000). NBT/BCIP developed animals were stored in 80% glycerol overnight prior to mounting ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies (anti-HA biotin conjugate 1:1000 (Roche), mouse anti-RAP148 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... 1:1000 mouse monoclonal anti-HA (3F10; Roche, 11867423001), 1:1000 rabbit polyclonal anti-RPN1 (abcam ...
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bioRxiv - Microbiology 2020Quote: ... and rat anti-HA at 1:1000 (Roche, 11867423001). After washing ...
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bioRxiv - Molecular Biology 2022Quote: ... rat-anti-HA (1:1000 for IB; Roche, ROAHAHA), rabbit anti-RECQL5 (1:1000 for IB ...
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bioRxiv - Cancer Biology 2022Quote: ... medium containing 1000 μg/mL Neomycin (G-418, Roche) was added to the cells ...
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bioRxiv - Microbiology 2023Quote: ... before application of Anti-His6-Peroxidase (Roche, 1:1000) in 5% (v/w ...
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bioRxiv - Plant Biology 2023Quote: ... or GFP (diluted 1:1000, catalog no. 11814460001, Roche). Alkaline phosphatase conjugated anti-rabbit ...
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bioRxiv - Genetics 2023Quote: ... 1:1000 anti-GFP antibody (Roche, # 11814460001, RRID: AB_390913) and 1:5000 goat anti-mouse IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2023Quote: ... and labeled with anti-HA (1:1000, 1hr; Roche). HA-tagged CDPK1 in the cWT and cMut lines was visualized with secondary goat antibodies (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... and rat-anti-HA (1:1000 dilution, Roche, 11867423001). Secondary antibodies used were Alexa-546-anti-rabbit (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Following antibody incubation (anti-GFP, 1:1000, Roche #118144600001), membranes were developed in ECL and imaged using the Chemidoc imaging system (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... 1:1000 dilution of 1mg/ml DAPI (Roche Diagnostics) was included during Poly-L-Lysine labeling ...
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bioRxiv - Developmental Biology 2019Quote: ... 2% blocking reagent (Roche), 20% heat inactivated goat serum and then incubated overnight with anti-DIG antibody (Roche ...
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bioRxiv - Immunology 2020Quote: ... The treated samples were purified using a C18 cartridge (Oasis HLB Plus Waters) prior to the release of N-glycans by PNGase F (recombinant from Escherichia coli, Roche) digestion ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR was performed on white 384-well plates (E&K Scientific, Santa Clara, CA) using Roche Lightcycler 480 (Roche Molecular Systems, Inc.). Each reaction contained 1 µL of cDNA from the RT reaction ...
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bioRxiv - Immunology 2023Quote: The retroviral packaging cell line Platinum-E was co-transfected with pMXs-SOX2-IP or pMXs-IP using the DNA Transfection Reagent X-TremeGene 9 (ROCHE, Cat# 06365779001) and following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The seeding of target cells was performed in 100 μl of medium per well to E-Plates 96 (Roche, Grenzach-Wyhlen, Germany), and appropriate cell densities were determined through titration experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere ...
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bioRxiv - Neuroscience 2023Quote: ... 150 μL of RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche, cat# A32953) was added to the tissue homogenate followed by incubation on a shaker for 1 h at 4ºC ...
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bioRxiv - Cell Biology 2024Quote: ... just after the resection the tissue was dissociated into small pieces (∼2 mm × 2 mm × 2 mm) and then digested for 30 minutes at 37°C with Collagenase (2 mg/mL, Roche, Basel, Switzerland) and DNase I (400 U/mL ...
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bioRxiv - Neuroscience 2020Quote: DNA from the patient and his parents was extracted from 100 μl of EDTA-anticoagulated whole blood using MagNA Pure (Roche Diagnostics, West Sussex, UK) and used for subsequent analyses.
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bioRxiv - Cell Biology 2020Quote: ... and IFNγ (1000 U/mL) (cat. 11040596001-Roche, Basilea, Switzerland) for 24 h.
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated with sheep-anti-DIG (1/1000, Roche) followed by biotinylated donkey-anti-sheep (1/500 ...
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bioRxiv - Immunology 2022Quote: ... using 1000 ng of RNA per sample (Roche, Basel, Switzerland). Quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2020Quote: ... and anti-fluorescein/DIG-Alkaline phosphatase (AP) (1:1000; Roche) antibodies were used ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies used: rat anti-HA 1:1000 (Roche 3f10) and anti-fibrillarin (ABCAM ab4566) ...
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bioRxiv - Neuroscience 2022Quote: ... sections were counterstained with DAPI (1:1000; Roche Applied Science) and then mounted ...
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bioRxiv - Molecular Biology 2020Quote: ... 1:1000 protease inhibitor pill (Roche: 04-693-132-001). For the preparation 40 g of lysed cell powder was used and a cytosolic S100 fraction was prepared ...
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bioRxiv - Cell Biology 2020Quote: ... The digestion buffer contains 1000 µg/ml Liberase TL (Roche), 100 U/ml DNase1 (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... Additional antibodies are rat anti-HA (Roche, #11867431001, 1:1000), mouse anti-NeuN (Chemicon #MAB377 1:2000) ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... or sheep anti DIG-POD (1:1000, Roche cat# 11207733910). Subsequently sections were incubated with Streptavidin-Cy2 and DAPI in blocking solution ...
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bioRxiv - Plant Biology 2022Quote: ... an antibody conjugate anti-DIG-AP (1:1000 dilution, Roche) and NBT/BCIP substrate were used for coloring reaction ...
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bioRxiv - Plant Biology 2022Quote: ... An antibody conjugate anti-DIG-AP (Roche, 1:1000 dilution) and NBT/BCIP substrate were used to visualize hybridization results ...
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bioRxiv - Cell Biology 2023Quote: ... anti-GFP (IB: 1: 1000, cat#11814460001, Roche, Basel, Switzerland) and anti-αtubulin (IB ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells were lysed by sonication on ice in PBS pH 7.4 with protease inhibitors (cOmplete, EDTA-free, Roche Applied Science). The protein was purified over Glutathione Sepharose 4 Fast Flow (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: The open-reading frame of the nikABCDE gene was amplified from genomic DNA belonging to Escherichia coli BL21(DE3) using the KAPA-HiFi Master Mix kit according to the manufacturer’s instructions (Kapa Biosystems, #KK2601). Primers are found in Supplementary Table 3 ...