Labshake search
Citations for Roche :
201 - 250 of 1949 citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... The resuspended pellet was layered carefully over 3 ml of S2 solution (0.35 M sucrose, 0.5 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) and centrifuged at 1430 × g for 5 min at 4°C ...
-
bioRxiv - Genomics 2024Quote: ... and the RT-qPCR was performed using ChamQ SYBR qRCR Master Mix (High ROX Premixed) (Cat# Q341-02-AA, Vazyme, China) by the LightCycler® 480 System (Roche, Switzerland) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2020Quote: ... The measurements were done on a Cobas e 311 (Roche, Switzerland). The inter-assay coefficient of variation was <1.65% using a frozen serum pool and <1.66% using quality control samples from Roche.
-
bioRxiv - Pathology 2020Quote: ... a sandwich electrochemiluminescence immunoassay ECLIA on Cobas e 411 (Roche, Switzerland). The inter-assay coefficient of variation was < 5.06% using a frozen serum pool and < 2.36% using quality control samples from Roche ...
-
bioRxiv - Cancer Biology 2021Quote: ... Insert amplification was performed using the Kappa Hi Fi Hotstart ReadyMix (Roche). For a 25 μL reaction ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: The recombinant biotinylated A33-His antigen was immobilized on streptavidin (StreptaWells, Roche) or avidin coated wells (Avidin ...
-
bioRxiv - Cancer Biology 2021Quote: ... Insert amplification was performed using the Kappa Hi Fi Hotstart ReadyMix (Roche). For a 25 μL reaction ...
-
bioRxiv - Microbiology 2020Quote: ... His-tagged CopS(34-151) was purified using Ni-NTA columns (Roche)(11) ...
-
bioRxiv - Biophysics 2022Quote: ... His-tagged ecMSG was loaded onto a gravity nickel affinity column (Roche) and eluted using 300 mM imidazole ...
-
bioRxiv - Molecular Biology 2022Quote: ... Hi-C libraries were prepared using KAPA LTP Library Preparation Kit (Roche) 65 with 12 amplification cycles ...
-
bioRxiv - Bioengineering 2021Quote: ... coli total tRNA from strain MRE600 was obtained from Roche Pharmaceuticals.
-
bioRxiv - Molecular Biology 2021Quote: ... GFP and Myc-tag were detected using anti-GFP (Ref. 11814460001, Roche) and anti-Myc (either Ref ...
-
bioRxiv - Molecular Biology 2020Quote: Rat monoclonal anti-HA tag antibody (clone 3F10) was purchased from Roche LifeScience (Penzberg ...
-
bioRxiv - Biochemistry 2022Quote: ... Coverslips were incubated with HA-tag primary antibody (1:200, Roche, 11867423001) in 0.1% BSA in PBS for 1 hour before subsequent incubation with antirat Alexa Fluor 546 secondary antibody (1:400 ...
-
bioRxiv - Biochemistry 2023Quote: ... blotted onto a PVDF membrane and probed with α-HA tag (Roche, Catalog# 11867431001 ...
-
bioRxiv - Cell Biology 2023Quote: ... using primer sets specific for OSGIN1 and OSGIN2 and from the Universal ProbeLibrary (Roche, see Table S2 for sequences). Analysis was done with Expression Suite software (Applied biosystems ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and DNA libraries were amplified with KAPA 2X Hi-Fi Hotstart Readymix (Roche).
-
bioRxiv - Cancer Biology 2021Quote: ... Full length cDNA amplification with Hi-Fi Hotstart Readymix (Roche, cat. no. 07959079001) was completed with a 98°C incubation then 21 cycles of (98°C for 15 sec ...
-
bioRxiv - Genomics 2021Quote: ... The Hi-C library was quantified using a KAPA library quantification kit (Roche), and further PCR amplification was performed using Phusion Hot Start II DNA polymerase (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2023Quote: ... After gap repair with Kapa Hi-Fi HotStart Uracil+ DNA Polymerase (KAPA Biosystems) and Taq DNA Ligase (NEB ...
-
bioRxiv - Genetics 2023Quote: ... or zebrafish genomic DNA using the Expand Hi-Fidelity PCR System (11732641001, Roche). Exact coordinates are listed in Supplemental Table 3 ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were in contact for 2 hours at room temperature with a mouse monoclonal primary antibody anti-BrdU (1/1000 dilution) (Roche), then rinsed and incubated for 30 min with a fluorescent secondary antibody Alexa-633nm goat anti-mouse (Invitrogen ...
-
bioRxiv - Neuroscience 2022Quote: ... The beads were then collected at 1000 g for 2 min and washed at least 3 times with lysis buffer coupled with protease inhibitor cocktail (Roche). After last wash and centrifugation ...
-
bioRxiv - Neuroscience 2021Quote: ... rat monoclonal anti-HA tag (clone BMG-3F10, Roche 12013819001, dilution 1:100); mouse monoclonal anti-Kv1.2 (clone K14/16 ...
-
bioRxiv - Biochemistry 2019Quote: The following antibodies were used in this study: anti-HA-tag (11867423001; Roche), anti-FlagM2-tag (F3165 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The let-A and I1B fragments were amplified from S2 cell genomic DNA using the Expand Long Template PCR System (Roche) and cloned in pBacPAK8_EGFP under a metallothionein promoter ...
-
bioRxiv - Developmental Biology 2022Quote: The different CRE candidate sequences were amplified using PCR-specific primers (see Table S2) and the Expand High Fidelity PCR system (Roche). Amplified fragments were gel-purified and cloned into the pSK-lacZ vector as in (Beccari et al. ...
-
bioRxiv - Developmental Biology 2022Quote: ... forward and reverse primers for the genes of interest (see Supplemental table S2) were mixed with FastStart Universal SYBR Green Master (Rox; Roche). 5 µl of this mixture was pipetted in duplicate per condition in a 384 well plate ...
-
bioRxiv - Microbiology 2020Quote: ... DNA probes for 5S rRNA and SPA0079 were amplified with PCR using primers described in Table S2 and PCR DIG Probe Synthesis Kit (#11636090910, Roche). The reaction was performed with a total volume of 50 μl containing 0.2 μM of forward primer ...
-
bioRxiv - Microbiology 2022Quote: ... Quantitative RT-PCR was performed using primers described in table S2 and KAPA SYBR Fast qPCR Master Mix (KAPA Biosystems) on a LightCycler 480 Instrument II (Roche) ...
-
bioRxiv - Genetics 2019Quote: Protein was isolated from 10 brain-imaginal disc complexes were dissected from wandering L3 larvae or S2 cells and homogenized in 1X Laemmli/RIPA buffer containing 1X protease inhibitors (Roche cOmplete Mini EDTA-free protease inhibitor tablets ...
-
bioRxiv - Developmental Biology 2020Quote: ... 5 pmol of the forward and reverse gene-specific primers each (primer sequences shown in table S2) in Light Cycler SYBR Green I Master mix (Roche) on LightCycler 480 II (Roche) ...
-
bioRxiv - Cancer Biology 2021Quote: ... The let-A and I1B fragments were amplified from S2 cell genomic DNA using the Expand Long Template PCR System (Roche) and cloned in pBacPAK8_EGFP under a metallothionein promoter ...
-
TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... was performed using gene specific primers (see File S2) in a total volume of 10 μl SYBR Green Master mix (Roche) on a LightCycler LC480 apparatus (Roche ...
-
bioRxiv - Immunology 2023Quote: ... Real-time PCR was performed using qPCR primer pairs listed in Table S2 and LightCycler480 SYBR Green I Master Mix (Roche) in a LightCycler480 instrument ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: The preparation of β-glucuronidase from Escherichia coli was from Roche Diagnostic (Mannheim ...
-
bioRxiv - Cell Biology 2022Quote: ... coli resuspensions were supplement with EDTA-free protease inhibitor tablets (Roche) and lysozyme prior to lysis via sonication ...
-
bioRxiv - Plant Biology 2023Quote: ... 1/1000 (Roche); HRP-conjugated anti-FLAG antibody ...
-
bioRxiv - Neuroscience 2023Quote: ... followed incubation with blocking solution for 2 hours before incubation with alkaline phosphatase (AP)-conjugated anti-DIG Fab fragments antibody (1:1000, Roche, #11093274910) overnight at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... targeting the indicated genes (Table S2) at a final concentration of 50 nM or 100 nM using X-tremeGene siRNA transfection reagent (Roche, 04476093001) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... About 20 g of bacterial cell pellet was resuspended in lysis buffer (100 mM Tris-HCl pH 7.5) supplemented with a tablet of EDTA-free cOmplete Protease Inhibitor S2 Cocktail Tablet (PIC) (Roche, Solna, Sweden) and DNase (PanReac Applichem ...
-
bioRxiv - Plant Biology 2021Quote: ... RT-qPCR primers (Table S1 and Table S2) were designed using the Universal Probe Library Assay Design Centre (Roche Life Sciences) and primer efficiency was determined using pooled cDNA samples ...
-
bioRxiv - Cell Biology 2022Quote: ... from BD Biosciences, mAb anti-HA-tag (11867423001; IF: 1/500, WB: 1/2000) from Roche, mAb anti-mRFP/DsRed2 (sc-101526 ...
-
bioRxiv - Microbiology 2023Quote: ... Amplified fragments with adapters and tags were purified using KAPA Pure beads (Roche, Switzerland) at a 1:1 concentration.
-
bioRxiv - Microbiology 2020Quote: ... coli Novablue were extracted using the High Pure RNA Isolation Kit (Roche) following the manufacturer’s protocol ...
-
bioRxiv - Genetics 2019Quote: ... coli strains using the High Pure plasmid isolation kit (Roche, Basel, Switzerland). PCR amplification of short DNA fragments (<3000 bp in length ...
-
bioRxiv - Microbiology 2020Quote: Escherichia coli MRE600 total transfer ribonucleic acid was purchased from Roche (Switzerland). Biotin labeled single-stranded DNA oligonucleotides were obtained from B.G.I. ...
-
bioRxiv - Physiology 2021Quote: ... 1:500 goat anti-rabbit (AlexaFlour 488; A-11008) and 1:1000 4′,6-diamidino-2-phenylindole (DAPI; Roche 10236276001; Basel, Switzerland). After secondary incubations ...
-
bioRxiv - Neuroscience 2023Quote: ... the cells were stained with secondary antibodies (1:300) for 2 hours at room temperature and nuclei stained with DAPI (1:1000, Roche, Munich, Germany). The cells were mounted using the ProLong Diamond antifade mountant (Thermo ...
-
bioRxiv - Cell Biology 2022Quote: ... Barcoded Illumina adaptor sequences (Table S2) were added to the tagmented DNA by PCR (KAPA HiFi HotStart ReadyMix, Kapa Biosystems, 12 cycles). And the DNA was cleaned with an x0.8 SPRI procedure ...