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Citations for Roche :
401 - 450 of 560 citations for Recombinant Human Membrane Metallo Endopeptidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... transferred to PVDF membranes and protein expression was detected using anti HA (3F10, Roche 11867423001, 1:5000) followed by horseradish peroxidase (HRP)-conjugated anti-rat antibodies (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were probed overnight at 4 °C with anti-GFP primary antibodies (Roche 11814460001; 1:1000) and then with anti-mouse peroxidase-conjugated secondary antibodies (Jackson Immunoresearch # 115-035-146 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked by incubation in 5% (w/v) milk power or 1 × Western Blocking Reagent (Roche) in Tris-buffered saline and 0.1% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were western blotted onto PVDF membranes and probed with the following antibodies: anti-HA (11867423001, Roche), anti-Flag (F3165 ...
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bioRxiv - Neuroscience 2020Quote: ... The DIG-labeled probes were hybridized to the membrane in DIG Easy Hyb hybridization buffer (Roche #11603558001) at 49°C for overnight ...
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bioRxiv - Neuroscience 2022Quote: ... For detection the membranes were shaken in 10 mL Lumi-light Western Blotting substrate (Roche, Cat. #12015200001) spiked in 1 mL SuperSignalTM West Femto Maximum Sensitivity Substrate (ThermoFischer ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was probed using the primary antibodies mAb mouse α-GFP (1:1’000, Roche Diagnostics #11814460001), mAb mouse α-PfGAPDH (1:20’000 ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated one hour in 5% milk before adding the primary antibody (anti-HA 3F10, Roche 27573500 ...
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bioRxiv - Neuroscience 2021Quote: ... Vector DNA was transiently transfected into human embryonic kidney (HEK) cells using Fugene-6 (Roche Molecular Biochemicals) transfection reagent ...
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bioRxiv - Immunology 2021Quote: ... Antibody protein treatments (anti-SARS-CoV-2 mAbs or human IgG1 isotype control Trastuzumab/Herceptin® (Roche)) were initiated 24 hours post infection by intraperitoneal injection ...
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bioRxiv - Immunology 2021Quote: Total RNA was retrotranscribed and cDNA was quantified using the Universal Human Probe Roche library (Roche Diagnostics). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... and goat anti-human AF647 (Stratech 109-606-088- JIR)) DNA was then stained with DAPI (Roche) and coverslips mounted with Vectashield (Vector Laboratories - Vector H-1000).
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bioRxiv - Immunology 2024Quote: Mice or Human CD4 T cells were lysed using RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Pierce) ...
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bioRxiv - Biophysics 2021Quote: ... Suspended membrane vesicles were diluted 1:2 in buffer EXT supplemented with protease inhibitors (Roche cOmplete EDTA-free) and 1% (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... the membrane was first probed with an anti-GFP antibody (Cat.No. 11814460001, Roche, Basel, Switzerland; 1:3’000 dilution) followed by a secondary anti-mouse-IR800 (R-05061 ...
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bioRxiv - Microbiology 2019Quote: Separated RNA was transferred from polyacrylamide gels to positively charged nylon membranes (Roche, cat. 11 209 272 001) using the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: Northern blotting was performed according to the standard protocol (23) using a positively charged nylon membrane (Roche, Switzerland). Before the transfer ...
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bioRxiv - Molecular Biology 2019Quote: ... The membrane was incubated overnight (O/N) at 4°C with mouse anti-α-tubulin (Roche, loading control) at 1:4000 ...
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bioRxiv - Biochemistry 2020Quote: RNA was separated on 12 % Urea-PAGE in 0.5x TBE and transferred to Nylon membranes (Roche, GE Healthcare) using semi-dry blotting in 0.5x TBE for 30 minutes at 5 V=constant ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to PVDF membranes and labelled overnight with anti-HA (0.01 μg/mL, Roche clone 3F10), anti-VE-Cadherin (0.5 μg.ml−1 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then probed overnight at 4℃ in anti-GFP (mouse monoclonal IgG1κ, cat#: 11814460001 Millipore Sigma/Roche) diluted 1:1000 in 5% Applichem nonfat dried milk in TBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were washed twice with ice-cold PBS containing cOmpleteTM protease inhibitor and phosSTOPTM phosphatase inhibitor cocktails (Roche). Cells were then gently lifted from membranes with cell scrapers (Corning ...
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bioRxiv - Microbiology 2021Quote: ... Equivalent amounts of protein samples were separated by SDS-PAGE and electroblotted onto a polyvinylidene fluoride membrane (Roche) using a Mini Trans-Blot Cell (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were blocked with 5% non-fat milk TBST and probed with primary anti-GFP (Roche, 1:1000) and secondary HRP- conjugated anti-mouse antibody (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were blocked with 10 mL of 1x blocking solution diluted in 1x Maleic Acid Buffer (Roche, 115857262001) with 0.3% TWEEN 20 for 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were blocked with 5% milk dissolved in TBST and probed with primary anti-HA (Roche, 1:2000), anti-Flag (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... intact Descemet’s membrane was stripped off the corneas and HCEnCs isolated using 1.5mg/ml Collagenase A (Roche, USA) in DMEM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was treated according to the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche) according to the manufacturer’s instructions using a DlG-labeled DNA probe corresponding to a single repeat of the KSHV terminal repeats as previously described13 ...
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bioRxiv - Biophysics 2021Quote: ... Isolated membrane fractions were diluted 1:2 in buffer EXT supplemented with protease inhibitors (Roche cOmplete EDTA-free) and 1% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Separated proteins were transferred to PVDF membranes (GE Water & Process Technologies) and treated with Western blocking reagent (Roche) for overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Equal quantities of protein were electrophoresed on 10% SDS-PAGE and transferred onto a nitrocellulose membrane (Roche Diagnostics). The membranes were incubated with the primary antibody overnight at 4°C after blocking with 5% milk dissolved in TBST buffer for another 2 h at room temperature (25°C) ...
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bioRxiv - Molecular Biology 2023Quote: ... DPCs were resolved on 5-18% SDS-PAGE gradient gels and transferred to a PVDF membranes (Roche, 03010040001). Membranes were blocked with 5% low-fat milk in TBST buffer (10mM TrisHCl (pH 7.5) ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were cut and immunodetected with either Horse Radish Peroxidase (HRP)-conjugated 3F10 anti-HA (1:2000, Roche) or HRP-conjugated Flag M2 (1:2000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Nucleic acids were UV-crosslinked to the membrane and incubated with prehybridization buffer DIG Easy Hyb (11603558001, Roche,) in a hybridization tube at 37° C for 30 min with rotation ...
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bioRxiv - Biophysics 2022Quote: ... Isolated membrane fractions were diluted 1:2 in buffer EXT supplemented with protease inhibitors (Roche cOmplete EDTA-free) and 1% (w/v ...
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bioRxiv - Plant Biology 2023Quote: ... transferred on nylon membrane and hybridized with DIG-labelled probes obtained by PCR DIG Probe Synthesis Kit (Roche) with specific primers (see table primers list) ...
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bioRxiv - Systems Biology 2023Quote: ... subsequently transferred to polyvinylidene fluoride membranes and probed with the anti-GFP primary antibody (Roche, Mouse monoclonal, #11814460001) and goat AffiniPure anti-mouse IgG (H+L ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was liberated from membrane fragments using 200µL of a 4mg/ml proteinase K solution (Roche, cat. #3115828001) diluted in PK buffer (100mM Tris-HCl ...
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bioRxiv - Plant Biology 2024Quote: ... RNA samples were separated on 1% agarose formaldehyde gels and transferred to Hybond-N membranes (Roche Molecular Biochemicals). Membrane hybridization was carried out overnight at 65°C using digoxigenin-labeled riboprobes corresponding to PVX CP sequences.
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were developed using enhanced chemiluminescence (Lumi Light or Lumi Light-plus, Roche/Sigma-Aldrich, St. Louis, MO) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... and were then stained with the mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) and the FITC-tagged secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: Human stem cells and neurons lysed with RIPA lysis buffer supplemented with 5mM EDTA and protease inhibitor (Roche), for 5 minutes at room temperature and 10 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... freshly resected human samples were cut into small fragments and digested with 0.1 mg/ml Liberase TL (Roche) and 0.1 mg/ml DNase (Roche ...
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bioRxiv - Developmental Biology 2021Quote: On day 0 (start of differentiation) human pluripotent stem cells were treated with 1mg/ml Collagenase B (Roche) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... human vimentin immunohistochemical staining of the paraffin sections was performed by using the Ventana Discovery XT instrument (Roche) with Ventana DAB Map detection Kit (760-124 ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were separated on 10%-15% SDS-PAGE gels and transferred to PVDF membranes (Roche, 03010040001). The membranes were probed with the indicated primary antibodies followed by the appropriate HRP-conjugated secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... The pellet (crude membrane preparation) was resuspended in 20mM Tris pH 8.0 plus protease inhibitor cocktail (Roche EDTA free), flash-frozen in liquid N2 and kept at -80°C.
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bioRxiv - Microbiology 2020Quote: ... The membrane was incubated with horseradish peroxidase (HRP)-conjugated rat anti-HA (clone 3F10) monoclonal antibodies (Roche, Indianapolis, IN) at a dilution of 1:5,000 for 2 hours ...
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bioRxiv - Plant Biology 2019Quote: ... Digested DNA was separated on 0.8% (w/v) agarose 1X TAE gel and electro blotted onto a positively charged nylon membrane (Roche), using 1X TBE as transfer buffer ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were blotted onto a nitrocellulose membrane and probed with anti-GFP (1:1000; Sigma-Aldrich and Roche), anti-dgt6 (1:500 ...