Labshake search
Citations for Roche :
551 - 600 of 2327 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... cells were transfected using XtremeGENE 9 (Roche), as per the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were transfected using XtremeGene9 reagent (Roche) according to the manufacturer’s instructions using an equal molar ratio of each construct ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were passaged using Dispase/collagenase (Roche) and DNase (Worthington Biomedical ...
-
bioRxiv - Genetics 2020Quote: ... Staining for apoptotic cells was performed using the AP-In situ Cell Death Detection Kit (Roche Diagnostics, Penzberg, Germany) following manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... The red blood cell layer was lysed with 2 mL of Red Blood Cell Lysis Buffer (Cat# 11814389001, Roche) in a 15 mL tube ...
-
bioRxiv - Systems Biology 2021Quote: ... cell culture counts were accessed in the count plate on an automated cell counter (Cedex, by Roche, Basel, Switzerland). As there was little or no growth lag after splitting the culture ...
-
bioRxiv - Cell Biology 2021Quote: ... were transfected into COS-7 cells (14,000-17,000 cells/cm2 in 12-well plate) for 24 h using X-tremeGENE HP DNA (Roche, #06366236001). Cells were incubated for 7 min at 37 °C with either 4 μg.mL-1 rabbit anti-γ2 Ex2 or 1:50 serum rabbit anti-γ8 Ex1 antibodies prior to fixation ...
-
bioRxiv - Cell Biology 2022Quote: ... The resulting cell suspension was subsequently passed through a 40μm strainer before cell counting using a Casey TT counter (Roche). Single cell suspensions (5 million cells/sample ...
-
bioRxiv - Microbiology 2020Quote: Cytotoxicity of the identified drugs on Vero E6 cell was determined with WST-1 cell proliferation assays (ROCHE, 5015944001). Twenty thousand cells were seeded into a 96-well plate and incubated for 20–24 h at 37 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... Apoptotic cells were detected in dissected guts using the TMR red In Situ Cell Death Detection Kit (Roche; 12156792910) following standard kit staining protocol ...
-
bioRxiv - Biophysics 2019Quote: ... 4 million cells were seeded in a 10 cm cell culture dish for transfection using X-tremegene 9 (Roche) and Opti-MEM (Gibco ...
-
bioRxiv - Biochemistry 2021Quote: ... The bacmid produced by DH10Bac cells was transfected into Sf9 cells using X-tremeGENE HP DNA Transfection Reagent (Roche), and then cultured at 27 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... 500 µl of 1x cell lysis buffer (Cell Signalling Technology) supplemented with Complete EDTA-free Protease Inhibitor Cocktail (Roche) was added for cell lysis ...
-
bioRxiv - Immunology 2022Quote: ... Apoptotic cells were detected in dissected guts using the TMR red In Situ Cell Death Detection Kit (Roche; 12156792910) following standard kit staining protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were harvested by cell scraping into 2 ml of PBS with a protease inhibitor cocktail (Roche cOmplete 4693159001). The cell suspension was centrifuge at 200xg for 10 min ...
-
bioRxiv - Molecular Biology 2022Quote: The psyllid midguts were diagnosed for cell-death or apoptosis using In situ cell-detection kit TMR-red (Roche) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: T cells were thawed into T-cell medium supplemented with 3,000 IU IL-2 (Proleukin) and DNAse (Pulmozyme, Roche) three to four days before coincubation with target cells ...
-
bioRxiv - Physiology 2023Quote: ... Cell death and DNA fragmentation were detected with a TUNEL In Situ Cell Death Detection Kit (Roche, Cat. 12156792910). Frozen pancreata for histology were removed and fixed as described above ...
-
bioRxiv - Physiology 2023Quote: ... Cell apoptosis was measured using an in situ cell death detection kit and following the manufacturer protocol (Roche, 12156792910). Images were obtained using an inverted fluorescent microscope (Leica DMi8 ...
-
bioRxiv - Cell Biology 2020Quote: ... generation of total protein from MEF cells was contained by homogenization of cells in lysis buffer containing protease inhibitor (Complete, Roche). For determination of G/F-actin ratio fractionation was performed as described in Huang et al ...
-
bioRxiv - Microbiology 2020Quote: Cell pellets of 1 × 106 cells were resuspended in 100 μl RIPA lysis buffer containing a protease inhibitor cocktail (Roche). Samples were centrifuged for 5 min to remove cell debris and stored at −20°C until use for Western blot analysis.
-
bioRxiv - Molecular Biology 2020Quote: ... Cell confluency was detected in real-time with the IncuCyte S3 Live Cell Analysis System (Roche Applied Sciences, Indianapolis, IN), with four captures per well every hour for 48 hours.
-
bioRxiv - Molecular Biology 2019Quote: ... cell pellet was incubated with Lysis buffer (ProteoJETTM Mammalian Cell Lysis Reagent, Fermentas) complemented with the Protease Inhibitor Cocktail (Roche), for 10 min at room temperature on a shaker (500 rpm) ...
-
bioRxiv - Immunology 2019Quote: ... lungs were removed and single-cell suspensions of lung mononuclear cells were prepared by Liberase Blendzyme 3 (70 ug/ml, Roche) digestion containing DNaseI (30 µg/ml ...
-
bioRxiv - Developmental Biology 2019Quote: TUNEL assay for scoring apoptotic cells was performed using In situ cell death detection kit fluorescein (Roche, Catalog No.11684795910), as per manufacturer’s protocol.
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche). Quality control of A33-His was performed by SDS-PAGE and size exclusion chromatography (Superdex75 10/300GL ...
-
bioRxiv - Cancer Biology 2021Quote: ... The Trypan blue exclusion method was performed to count the total number of viable tumor cells in the peritoneal cavity using the Cedex cell counter (Roche). Compared to the control (Group II) ...
-
bioRxiv - Neuroscience 2021Quote: ... Detection and quantification of apoptosis in cell cultures was determined using a In Situ Cell Death Detection Kit (Roche, 11684795910) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Cell toxicity at different concentrations of drugs as indicated was determine using the Cell Proliferation Reagent WST-1 (Roche Diagnostics) or cellular 96® Aqueous One Reagent viability reagent according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... CAL33-c5 and CAL33-c11were seeded in 96-well plates and cell viability was determined with the colorimetric Cell Proliferation Kit II (XTT) (Roche) 24 ...
-
bioRxiv - Microbiology 2020Quote: Whole cell lysates were obtained by lysing cells in RIPA buffer supplemented with a cOmplete Mini protease inhibitor cocktail (Roche). Protein concentrations were measured using a BCA assay (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2019Quote: ... GFP-AURKA-GFP and GFP-AURKA Lys162Met-GFP stable clones cells were generated by transfecting U2OS cells with X-tremeGENE HP transfection reagent (Roche), following the manufacturer’s indications ...
-
bioRxiv - Microbiology 2020Quote: The cell viability of circPSD3 siRNA treated cells was determined at day 2 post-transfection using Cell Proliferation Kit I (MTT, Roche) as previously described [59].
-
bioRxiv - Biochemistry 2021Quote: ... 2×106 cells HEK293T cells were transfected with 2 μg plasmid DNA using X-tremeGENE HP DNA Transfection Reagent (Roche) in 10-cm dishes ...
-
bioRxiv - Immunology 2021Quote: ... of SARS-CoV-2 was determined by incubating the virus in a serial dilution manner with the Vero cell line (CCL81, ATCC) in gold microelectrodes embedded microtiter wells in XCELLIGENCE Real-Time Cell Analysis (RTCA) instruments (ACEA, Roche) for 8 days ...
-
bioRxiv - Immunology 2021Quote: ... of SARS-CoV-2 was determined by incubating the virus in a serial dilution manner with the Vero cell line (CCL81, ATCC) in gold microelectrodes embedded microtiter wells in xCELLigence Real-Time Cell Analysis (RTCA) instruments (ACEA, Roche) for 8 days (Sir Karakus et al ...
-
bioRxiv - Cancer Biology 2022Quote: PC3 cell proliferation was quantified by an MTT based colorimetric assay using the Cell Proliferation Kit I (Roche, Sigma Aldrich). WT ...
-
bioRxiv - Microbiology 2022Quote: ... HeLa BioID-RSK cells were obtained by transduction of HeLa-RSK-DKO cells with BLP10 and transduced cell populations were then selected with 1mg/ml of G418 (Roche). HeLa WT-RSK ...
-
bioRxiv - Neuroscience 2023Quote: Apoptotic cell death was assessed as formation of fragmented nucleosomes using the Cell Death Detection ELISAPlus kit (Roche Applied Science) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: For nuclear cell lysis 5×10^6 cells per condition were resuspended in 1mL of Lysis Buffer + protease inhibitor (cOmplete, Roche) (10mM Tris-HCl pH 7.4 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were lysed in NP40 cell lysis buffer (10mM Tris-Cl, pH 7.4, 1% NP40, 1X Roche Complete protease inhibitors) at 4C for 1 hour followed by centrifugation at 18,000 g for 10 mins and supernatant collected as sample for analysis ...
-
bioRxiv - Microbiology 2023Quote: ... To prevent adherence of cells to one another by DNA leakage the suspension of cells was incubated with 600 Units of DNAse I (Roche) (30 mins ...
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 5 μg of plasmid and dsRNA were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) every 3 days four times ...
-
bioRxiv - Immunology 2023Quote: Cells were washed with PBS and lysed with ice-cold CelLytic M Cell Lysis Reagent containing protease inhibitor cocktail (Roche) and phosphatase inhibitors (Sigma) ...
-
bioRxiv - Pathology 2023Quote: ... in HEK 293T cells (3×105 cells per well in 6-well plates) using X-treme gene transfection reagent (Roche). Pseudotyping was achieved by co-transfecting pHEF-VSVg (400 ng/well) ...
-
bioRxiv - Cancer Biology 2023Quote: ... HEK-293T and MEF cells (except for p62 KO MEF cells) were transfected with plasmids using X-tremeGENE 9 (Roche) as described as previously (41–43) ...
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
-
bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were counterstained with DAPI (Roche, 10236276001) and coverslips embedded in fluorescent mounting medium (Dako ...
-
bioRxiv - Cell Biology 2020Quote: ... MelJuSo cells were transfected using Extremegene HP (Roche) (500ng DNA per well in 24 well plate) ...