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Citations for Roche :
251 - 300 of 5494 citations for Protein Labeling kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The probe was amplified by PCR with digoxigenin-dUTP from the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Diagnostics), using the glms sequence as a template ...
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bioRxiv - Systems Biology 2020Quote: ... We generated digoxigenin (DIG)-labeled mRNA antisense probes against Nrxn3 using reverse-transcribed mouse cDNA as a template and an RNA DIG-labeling kit from Roche (Sigma). Primer and probe sequences for the Nrxn3 probe is available in Allen Brain Atlas (http://www.brain-map.org) ...
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bioRxiv - Genetics 2019Quote: ... Digoxigenin-labeled sense and antisense probes were synthesized using linearized pGEM-Teasy vector subcloned with this thoc1 fragment by in vitro transcription with DIG-RNA labeling Kit (Roche, Switzerland). Zebrafish embryos and larvae were collected and fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Plant Biology 2019Quote: ... The probes were labeled with digoxigenin (DIG) according to the manufacturer’s protocol (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche, Basel, Switzerland). Northern blot procedures were performed as previously described (Jiang et al. ...
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bioRxiv - Plant Biology 2019Quote: ... were used as templates to synthesis DIG-labeled probes according to the manufacturer’s protocol (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche, Basel, Switzerland). ULTRAhyb®-Oligo Hybridization Buffer (Ambion ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then hybridized overnight at 70 °C with a digoxigenin (DIG)-labeled RNA probes (DIG RNA labeling kit, Roche 11277073910) for Gadd45b mRNA ...
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bioRxiv - Molecular Biology 2021Quote: Random primed DNA probes were generated by using the Random Primed DNA Labeling Kit from Roche (cat# 11-004-760-001). Generally ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcription was performed on the pcr product with Ampliscribe T7-flash kit with Dig RNA labeling mix from Roche(75). In situ hybridization with both the anti-sense probes yielded the same results.
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bioRxiv - Immunology 2023Quote: ... Apoptosis also was detected using a terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end-labeling (TUNEL) assay with Cell Death Detection Kit (Roche, Germany). Nuclei were stained with 4-6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2023Quote: ... Southern blotting was conducted to confirm the correct deletion using the digoxigenin (DIG) high prime DNA labeling and detection starter Kit I (11745832910 Roche Germany). The Myb gene complementation vectors were constructed by cloning the entire length of the target gene with the native promoter region (about 1.5-kb ...
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bioRxiv - Neuroscience 2023Quote: Plasmids were obtained from Transomic Technologies and the antisense mRNAs were synthesized using the Digoxigenin RNA Labeling Kit (SP6/T7; Roche, 11175025910). DIG RNA in situ hybridization (ISH ...
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bioRxiv - Zoology 2023Quote: The PCR products amplified from both IHHNV-309 and IHHNV-389 methods were used as templates to prepare digoxigenin (DIG)-labeled IHHNV probes using a PCR DIG-labeling kit (Roche, Germany). After preparation ...
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bioRxiv - Neuroscience 2023Quote: ... and Vglut3 and fluorescein (FITC)-labeled mRNA against DBH using reverse-transcribed mouse cDNA as a template and an RNA DIG or FITC-labeling kits from Roche (Sigma). Primer and probe sequences for the Vglut1 ...
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bioRxiv - Cell Biology 2023Quote: ... Digoxigenin (DIG)-labeled anti-sense mak RNA probe was generated by in vitro transcription from this T7 promoter: mak cDNA fragment as the template using a DIG RNA labeling kit (Roche, 11175025910). Zebrafish embryos from the 1-cell stage to 72 hpf were fixed with 4% PFA at 4 °C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... The digoxigenin (DIG)-labeled sense or antisense RNA probe for full-length Emi2 was synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals). No signal was detected with sense probes ...
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bioRxiv - Developmental Biology 2021Quote: ... The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals). Two μg of probes was incubated with purified Flag-tagged proteins for 15 min ...
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bioRxiv - Evolutionary Biology 2021Quote: Two-color in the fluorescence in situ hybridization experiment was performed following the instructions of DIG RNA Labeling Kit (Roche, Mannheim, Germany). When synthesizing probe ...
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bioRxiv - Plant Biology 2020Quote: ... washing and detection were performed according to instructions of the DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche, Mannheim, Germany).
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bioRxiv - Plant Biology 2020Quote: ... antisense RNA probes were generated using a DIG-labeling kit and the resulting probes hydrolyzed as previously described (Roche Diagnostics, IN, USA) (36) ...
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bioRxiv - Microbiology 2021Quote: ... Southern blot was performed using the DIG High Prime DNA Labeling and Detection Starter kit I according to the manufacturer’s instructions (Roche Diagnostics, Mannheim, Germany). The specific sequence was amplified from Hph gene using primer pairs (Supplemental Table S2) ...
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bioRxiv - Molecular Biology 2022Quote: ... According to the manufacturer’s protocol for Southern blot hybridization and detection digoxigenin-labeled hybridization probes (DIG DNA Labeling and Detection Kit, Roche Applied Science, 11175033910) were used ...
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bioRxiv - Neuroscience 2020Quote: The extent of cell death following MPP+ treatment with and without LMX1B overexpression was assessed by using the terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end-labeling (TUNEL) method (In situ cell death detection kit, TMR red, Roche, Cat# 12156792910), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... Partial f5 cDNA fragments were amplified to synthesize sense and antisense digoxin-labeled RNA riboprobes (DIG RNA labeling kit, Roche, Basel, Switzerland) (Table 1) ...
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bioRxiv - Bioengineering 2019Quote: ... Hybridization and washing were performed according to the procedures of DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche, Mannhein, Germany). The membranes were imaged using UVP software.
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bioRxiv - Molecular Biology 2019Quote: ... and the operating procedures according to the protocol supplied by the manufacturer of DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche, Mannheim, Germany). The prober primes are shown in supplementary (Table S3).
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bioRxiv - Microbiology 2019Quote: Southern blot was performed using a digoxigenin (DIG) High Prime DNA Labeling and Detection Starter Kit I (Roche Diagnostics GmbH, Mannheim, Germany) as instructed by the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... The T7 promoter was added to the 5’ and 3’ ends to synthesize the sense and anti-sense probes using the DIG RNA Labeling Kit (Roche, Cat# 11175025910). In situ hybridization was performed as previously described [65,66] with minor modifications ...
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bioRxiv - Cell Biology 2020Quote: ... Southern blotting was performed as instructed in the instruction manual for the DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche Cat. 11585614910).
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bioRxiv - Evolutionary Biology 2021Quote: ... and the product was used for the second round PCR using the primers F and R-T7 for generating antisense probe and primers R and F-T7 for generating sense probe used in ISH assays with the DIG RNA Labeling Kit (Roche, Mannheim, Germany),the primers were shown in table 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA probes were synthesized by in vitro transcription under the drive of SP6 promoter with the DIG RNA Labeling Kit (Roche, Mannheim, Germany); Then ...
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bioRxiv - Immunology 2021Quote: ... it was added 50 µL of fresh TUNEL reaction mixture composed of 5 µL of enzyme solution mixed with 45 µL of labeling solution (In Situ Cell Death Detection kit, POD, ROCHE, version 15.0) for 1 hour at 37 °C ...
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bioRxiv - Genetics 2020Quote: ... The membrane was hybridized using digoxigenin-labeled DNA probes (S4 Table) for mPSP based on the DIG-High Prime DNA Labeling and Detection Starter Kit II protocol (Roche, Mannhein, Germany). For western blotting ...
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bioRxiv - Developmental Biology 2022Quote: ... in situ terminal deoxynucleotidyl transferase deoxyuridin triphosphate nick end labeling (TUNEL) assay was performed using in situ cell death kit from Roche (Basel, Switzerland).
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bioRxiv - Developmental Biology 2022Quote: ... Antisense and sense RNA probes of TaDOF5.6 and TaDOF3.4 genes were synthesized using a DIG RNA Labeling Kit (SP6/T7) (Roche, Cat No. 11175025910, USA). The oligonucleotide primers for in situ hybridization are listed in Table S28.
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bioRxiv - Physiology 2022Quote: ... The PCR products of target genes were purified and used as templates for probe preparation using DIG or fluorescein RNA labeling kits (Roche, Basel, Switzerland). The pituitary sections were hybridized with the antisense RNA probes for fshb and lhb as we previously reported [52] ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated at 37 °C in the dark for 1 h with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) solution from the In Situ Cell Death Detection Kit (Roche, Mannheim, Germany). The embryos were washed three times with PBS-PVP ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... or T3 polymerase (pSC-best4, pSC-otop2) and fluorescein or digoxygenin RNA labeling kits (digoxygenin: Roche, Cat# 11277073910; fluorescein: Roche, Cat# 11685619910) These reactions were cleaned using the NEB Monarch RNA Cleanup protocol (NEB ...
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bioRxiv - Pathology 2023Quote: Terminal deoxynucleotidyl transferase-mediated digoxigenin-deoxyuridine nick-end labeling (TUNEL) staining was performed by using the In Situ Cell Death Detection Kit from Roche (Mannheim, Germany). Briefly ...
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bioRxiv - Genetics 2023Quote: The TUNEL assay was performed to detect apoptosis using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and In-Situ cell death detection Fluorescein kit (Roche Cat#11684795910), as published ...
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bioRxiv - Pathology 2023Quote: Cell death was assessed in tissue sections using terminal deoxynucleotidyl transferase-mediated dUTP nick- end labeling (TUNEL, Roche TUNEL kit (12156792910; Roche, Basel, Switzerland), as previously described (Cade et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Deletion of FGSG_09786 was further verified by Southern blot with the Digoxigenin High Prime DNA Labeling and Detection Starter Kit I (Roche, Mannheim, Germany). Using a similar strategy ...
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bioRxiv - Microbiology 2024Quote: ... Gene-specific antisense or sense DIG-labeled cRNA probes were obtained by in vitro transcription with T7 polymerase using the Roche DIG RNA labeling kit (Roche Diagnost ics) following the instructions of the supplier ...
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bioRxiv - Cancer Biology 2024Quote: Terminal dUTP Nick End Labeling (TUNEL) staining was performed with an In Situ Cell Death Detection Kit (TMR Red #12156792910; Roche Applied Science) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Digoxigenin-labeled riboprobes were synthesized with DIG RNA Labeling Mix (ROCHE), Thermo T7 RNA Polymerase (ToYoBo) ...
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bioRxiv - Genomics 2019Quote: ... They were alternatively labeled using the Dig RNA labeling mix (Roche) or the biotin RNA labeling mix (Roche) ...
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bioRxiv - Genetics 2019Quote: ... along with DIG RNA labeling mix and T7 RNA polymerase (Roche Diagnostics Corporation ...
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bioRxiv - Systems Biology 2019Quote: ... for in situ probes with digoxigenin or FITC labeling mix (Roche), respectively ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... with 2 µL of the Fluorescein labeling mix (Roche, Basel, Switzerland), instead of the recommended 2 µL of each NTP ...
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bioRxiv - Pathology 2020Quote: ... the sequence was labeled with digoxigenin using the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Diagnostics Deutschland GmbH, Germany). After dewaxing in water ...
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Dissemination of linezolid-resistance through sex pheromone plasmid transfer in Enterococcs faecalisbioRxiv - Microbiology 2019Quote: ... blots were hybridized with digoxigenin (DIG)-labeled optrA-specific probe using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche Applied Sciences, Germany) following the manufacturer’s instructions.