Labshake search
Citations for Roche :
201 - 250 of 5494 citations for Protein Labeling kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... subtilis strains at the different time points were evaluated via terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) using the In-Situ Cell Death Detection Kit with fluorescein (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The 32P-radiolabeled probe was generated from a plasmid containing the entire HPV16 genome by radiolabeling using a Random Prime labeling kit (Roche).
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bioRxiv - Plant Biology 2020Quote: ... The amplified fragments were then used to produce digoxigenin (DIG)-labeled sense and antisense RNA probes using a DIG RNA Labeling Kit (Roche). Whole-mount in situ hybridization was performed following (39) ...
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bioRxiv - Microbiology 2020Quote: ... the DNA was subjected to pre-hybridization and hybridization using a DIG High Prime DNA labeling and detection starter kit II (Roche) according to the manufacturer’s instructions with a DIG-labeled probe of ∼100-200 bp annealing in the targeted gene (for primer sequences ...
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bioRxiv - Biochemistry 2022Quote: ... samples were incubated with 50 μl mix of 5 μl enzyme and 45 μl labeling solution (TUNEL In situ Cell Death Detection Kit, TMR Red’ from Roche) for 1.5 h at 37°C in a dark humid chamber ...
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bioRxiv - Developmental Biology 2022Quote: ... Riboprobes labeled with digoxigenin (DIG), fluorescein (FLU) or dinitrophenol (DNP) were prepared as described (Opitz et al., 2011) using DIG RNA labeling kit (Roche), FLU RNA labeling kit (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR products were then purified and reverse transcribed with digoxigenin labeling using a DIG RNA labelling kit (Roche, Switzerland). For dissected adult pituitary or 5-8 dpf larvae ...
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bioRxiv - Genetics 2019Quote: ... 1 ug purified digested plasmid was used as template for SP6 or T7 transcription using the DIG-RNA labeling kit (Roche). Following probe transcription ...
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bioRxiv - Neuroscience 2021Quote: The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay (Gavrieli, Sherman, and Ben-Sasson 1992) was performed using the fluorescein kit from Roche Diagnostics (Mannheim ...
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bioRxiv - Genomics 2020Quote: ... These DNA fragments were labeled with digoxigenin (DIG) using a DIG High Prime DNA Labeling and Detection Starter Kit II (Roche), and signals were detected according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: ... Antisense or sense strand digoxygenin-UTP labeled RNA was generated for PEPCK and NADP-ME using a DIG RNA labeling kit (Roche). For PEPCK hybridizations ...
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bioRxiv - Neuroscience 2021Quote: Digoxigenin RNA-labeled or Fluorescein RNA-labeled probes were transcribed in vitro using the RNA Labeling Kit (Roche Diagnostics Corporation) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: The in vitro synthesis of digoxigenin (DIG)-labeled antisense SatIII probe (158 bp SatIII repeat cloned in 1μg of PGEM-2-98 construct) with T7 RNA polymerase was carried out using the DIG labeling kit from Roche Products Pvt ...
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bioRxiv - Immunology 2021Quote: ... Synthesized DNA specific to regions of homologous recombination (see Oligonuleotide section in Key Resources Table) was labeled with 32P (Amersham) using the High Prime labeling kit (Roche) according to manufacturer’s instructions and unincorporated 32P was removed with G-50 Sephadex Quick Spin columns (Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Alkaline hydrolysis was then performed at 60°C for 30 min to fragment the target RNA before labeling it with Digoxigenin and Fluorescein according to kit instructions (Roche).
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bioRxiv - Molecular Biology 2022Quote: ... The blots were hybridized with the MVC VP2 genome probe using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed using the In Situ Cell Death Detection Kit (Roche) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Digoxigenin (DIG)-labeled RNA probes were transcribed in vitro using a DIG RNA labeling kit (Roche Applied Science, Basel, Switzerland) and brain sections were hybridized with gene-specific antisense RNA probes ...
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bioRxiv - Developmental Biology 2022Quote: 155BS-upd from Doug Harrison and RE35237 from BDGP cDNAs were used to generate upd and sna RNA probes using the DIG RNA Labeling Kit (Roche). Secondary biotinylated antibody against mouse (1:200 ...
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bioRxiv - Plant Biology 2022Quote: ... Sense and anti-sense digoxigenin-labelled probes were synthesised using the DIG RNA Labeling Kit (SP6/T7; Roche, Basel, Switzerland) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... The RNA level was detected using the DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche, Cat. 11585614910).
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Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... The antisense RNA probes corresponding to the 3’ s-end portion of glnA CDS were prepared by the DIG RNA Labeling Kit (Roche).
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bioRxiv - Plant Biology 2022Quote: ... derived from SlARF2A and SlARF2B promoter was labeled with digoxigenin as per the manufacturer’s protocol using DIG Oligonucleotide 3′-End Labeling Kit (Roche Diagnostics). The same unlabeled DNA fragment was used as a competitor ...
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bioRxiv - Neuroscience 2023Quote: ... 2008) using commercial transcription kits and RNA labeling mix (REF #: 11277073910, Lot #: 26591021 & REF #11426346910:, Lot # 24158720; Roche Applied Science) to generate digoxigenin-labeled and fluorescein-labeled riboprobes using a plasmid template containing cDNA ...
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bioRxiv - Neuroscience 2022Quote: ... Antisense and sense RNA probes were synthesized from the linearized plasmid template by in vitro transcription using a DIG RNA Labeling Kit (Roche). In situ hybridization was performed on cryo-sections of telencephalon as previously described (Masai et al. ...
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bioRxiv - Microbiology 2022Quote: ... and visualized with anti-DIG-AP antibody according to the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche) instructions on a Chemidoc MP.
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bioRxiv - Microbiology 2023Quote: ... and the membrane was treated with the DIG-High Prime DNA Labeling and Detection Starter kit II (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: Antisense probes were synthesized using purified PCR products (1 μg per reaction) (supplementary table S16) and DIG RNA labeling Kit (T7) (Roche), following the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: Apoptotic cells were identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining using the In Situ Cell Death Detection Kit (Roche) according to the manufacturer’s protocol ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... and in vitro transcribed using T7 (pSC-pbx3a, pSC-cdx1b) or T3 polymerase (pSC-best4, pSC-otop2) and fluorescein or digoxygenin RNA labeling kits (digoxygenin: Roche, Cat# 11277073910 ...
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bioRxiv - Molecular Biology 2023Quote: 100 pmol gapmers (Supplemental Table S4) were labeled with biotin-16-ddUTP (Jena) and a 2nd generation DIG-oligonucleotide 3’end-labeling kit (Roche) using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNAs were labeled with [α32P]-dCTP using the Random Primed DNA Labeling Kit following the protocol provided by Roche. Total RNA was TRizol (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxigenin (DIG)-labeled sense and antisense riboprobes were prepared using the in vitro transcription DIG labeling kit (Roche, IN, USA). Hybridization was carried out at 600C overnight and the slides were washed and probed with an alkaline phosphatase-conjugated anti-DIG antibody (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was done following manufacturer’ s instruction (In Situ cell Death Detection Kit TMR red, Roche).
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bioRxiv - Developmental Biology 2023Quote: ... Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay was performed using the In Situ Cell Death Detection Kit (11684795910, Roche) and ...
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bioRxiv - Developmental Biology 2023Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed using the In Situ Cell Death Detection Kit (11684795910, Roche) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Digoxigenin (DIG)-labeled sense and antisense probes were generated with T7 and Sp6 RNA polymerase using a DIG RNA labeling kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... labeled antisense riboprobes were generated from the cDNA template using T7 RNA polymerase and a DIG-UTP RNA labeling kit (Roche). Brain sections were fixed in 3% phosphate buffered paraformaldehyde for 5 min followed by a rinse in phosphate buffered saline ...
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bioRxiv - Molecular Biology 2024Quote: ... To detect for DNA containing complementary sequences on membrane-bound DNA a α-32P-dCTP-labelled probe spanning the region of 37-611 nts on human mtDNA was synthesized using High Prime DNA Labeling Kit (Roche). After pre-hybridizing the membrane with Church’s buffer (250 mM NaPi pH 7.2 ...
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bioRxiv - Cancer Biology 2024Quote: The Terminal dUTP Nick End Labeling (TUNEL) and In Situ Cell Death Detection Kits (TMR Red #12156792910; Roche Applied Science) were used to examine apoptosis ...
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bioRxiv - Pathology 2021Quote: ... a commercial PCR DIG-labeling mix (Roche Molecular Biochemicals, Germany). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... fixed frozen sections were incubated with TUNEL labeling mix (Roche) at 37°C for 1 hour and then washed with PBST and mounted in DAPI containing medium (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... and a biotin RNA labeling mix (cat. no. 11685597910, Roche). To create a 20 μl reaction volume ...
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bioRxiv - Developmental Biology 2024Quote: ... or the Fluorescein RNA labeling mix (Roche, Sigma product # 11685619910) to facilitate double staining.
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bioRxiv - Neuroscience 2020Quote: ... The resulting PCR products were purified and used as templates for in vitro transcription by employing DIG RNA Labeling Kit (SP6/T7) (Roche #11175025910). The resulting RNAs generated from SP6-mediated in vitro transcription were used as positive probes (cRNA ...
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bioRxiv - Cancer Biology 2020Quote: Cell (1 × 104) proliferation was measured using the 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit III (Roche, Mannheim, Germany) (Jin ...
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bioRxiv - Plant Biology 2021Quote: ... Hybridization was conducted according to the procedures described in the DIG High Prime DNA Labeling and Detection Starter kit II protocol (Roche®), using a 725-bp C-terminal TalCMAI1 amplicon as probe (Yu et al ...
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bioRxiv - Zoology 2021Quote: ... a fragment of the EHP small subunit ribosomal RNA (SSU rRNA) gene was used to generate an EHP probe with a PCR-DIG labeling kit (Roche, Germany) ENF779 and ENR779 (Tangprasittipap et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The stained cells were further labelled by TUNEL (TdT-mediated dUTP-X nick end labeling) with an In-Situ Cell Detection Kit (TMR red) from Roche (12156792910). Fluorescent images were collected on a Zeiss Automatic stage microscope with Zen blue software ...
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bioRxiv - Genetics 2022Quote: ... Hybridization was performed as described in the Instruction Manual of the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Diagnostics). Washing conditions was performed according to Huang et al (Huang et al ...