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Citations for Roche :
501 - 550 of 2419 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The sections were immediately covered with 20-60 µL primary antibody in Triton-Wash buffer (20mM HEPES pH 7.5,150mM NaCl, 2mM spermidine and Roche complete EDTA-free protease inhibitor) added dropwise ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded human intestinal tissues was carried out on a Discovery Ultra automated slide stainer using a rabbit anti-human monoclonal antibody for CEA (Clone T84.66, Roche Glycart AG, Switzerland) at 2.23 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Microbiology 2020Quote: ... and 3-4 mice/group subcutaneously received 25ng/g of pegylated-human interferon α (Peg-hIFNα-2a) (Hoffmann La Roche, Basel, Switzerland). To activate IFN-1 signaling ...
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bioRxiv - Biophysics 2019Quote: ... or for confocal in a alkaline-washed glass-bottomed chamber coated with human plasma fibronectin (25 µg/mL at 4°C overnight in 1/3 X MBS; Roche Molecular Biochemicals) in DFA supplemented with antibiotic/antimycotic (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: Ovaries and testis were fixed and stained according to standard procedures with the following primary antibodies: rat anti-HA (1:500, Roche cat# 11867423001, RRID: AB_390919), rat anti-Vasa (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed using 10X Cell Lysis Buffer (Cell Signalling) supplemented with protease and phosphatase inhibitors (Roche). Protein concentration was determined using a BCA protein assay kit (Pierce ...
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bioRxiv - Molecular Biology 2021Quote: ... cell viability was assessed using Cell Proliferation Kit I (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cell growth was monitored using a CASY cell counter (Roche). PCR products or plasmids linearized by NotI were transfected into cells by electroporation (Biorad) ...
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bioRxiv - Cell Biology 2024Quote: ... Cell growth was monitored using a CASY cell counter (Roche). Expression of GFP fusion proteins and RNAi were induced with doxycycline at a final concentration of 10 ng/mL and 1 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Cell growth was monitored using a CASY cell counter (Roche). PCR products or plasmids linearized by NotI were transfected into cells by electroporation (Biorad) ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were blocked in 10% BSA and 0.05% Tween 20 in PBS for 30 minutes before application of one of the following antibodies: mouse anti-human p16INK4a (CINTEC-Roche, cat# 705-4793), rabbit anti-human alpha-smooth muscle actin or aSMA (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... frozen human samples were disrupted in 600 µl of lysis buffer containing green ceramic beads using the MagNA Lyser Instrument (Roche Life Science, Germany). The cDNA synthesis was performed in a 20-µl reaction using random hexamers ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of beta-globin was performed by a commercially available human genomic DNA kit (The LightCycler Control Kit DNA, Roche Diagnostics, Basel, Switzerland)69.
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bioRxiv - Systems Biology 2020Quote: ... Cell counting and assessment of cell viability were performed using the Cell Counter and Analyzer System (CASY, Roche).
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation in LNCaP IL1B overexpression and vector cells was assessed using Cell Proliferation Reagent WST-1 (Roche), per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Cells proliferation was assessed by BrdU Cell proliferation ELISA from Roche. IL-6 ELISA was from Affimetrics ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was evaluated using a colorimetric Cell Proliferation ELISA (Roche), based on the measurement of the incorporation of bromodeoxyuridine (BrdU ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell numbers were monitored using the CASY automated cell counter (Roche).
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bioRxiv - Biochemistry 2023Quote: Cell proliferation was determined using a colorimetric Cell Proliferation ELISA (Roche) kit ...
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bioRxiv - Immunology 2021Quote: Single-cells were sorted into PCR tubes containing 1μl of cell lysis solution (1:10 Cell Lysis buffer(Roche), 10U/μl Rnasin plus Ribonuclease inhibitor (Promega ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG; or anti-GFP, 1:1000, Roche #11814460001 in 3% BSA/TBS-T) at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were harvested by centrifugation and red blood cells were removed using a red blood cell lysis buffer (Roche). Samples for intracellular staining were stained with zombie red for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: Viability and cell growth ratios were determined analyzing cell density by MTT using the Cell Proliferation Kit I (Roche, 11465007001) in DND41 cells treated with EX-527 (10-6 to 10-3 M ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then counted using a CASY cell counter (Roche, Basel, Switzerland). Final cell number was normalised to the initial cell count obtained at T0 ...
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bioRxiv - Cancer Biology 2022Quote: ... the cell pellet was resuspended in red blood cell lysis buffer (Roche) and incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... Red blood cells were lysed using red blood cell lysis buffer (Roche), after which cells were again washed twice with cold AdDF+++ ...
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bioRxiv - Biophysics 2020Quote: ... cell viability was determined using Cell Proliferation Reagent WST-1 (Roche, 5015944001) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Cell proliferation was assessed by Cell Proliferation ELISA BrdU kit (11647229001, Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... before cell numbers were determined using a CASY TT Cell Counter (Roche). 4×106 cells were transferred to 96-well plates for antibody staining ...
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bioRxiv - Cancer Biology 2022Quote: ... B cells were thawed in B cell medium containing DNAse (Pulmozyme, Roche) 20 h before use in co-culture assays ...
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bioRxiv - Bioengineering 2023Quote: ... cell viability was measured by the MTT assay (Cell proliferation kit, Roche Diagnostics Deutschland GmbH ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability was assessed using the MTT cell proliferation kit I (Roche), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...
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bioRxiv - Cell Biology 2022Quote: Whole protein lysates were isolated from iPS cells and iPS-ECs using the 1x Cell Lysis buffer (Cell Signalling, 9803) supplemented with protease inhibitors (Roche 11697498001). Cells were washed using PBS and harvested at the same passage and confluency (80% ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were centrifuged and cell killing assessed using the LDH Assay (Roche #11644793001) according to the manufacturers protocol using the SPECTROstar Nano (BMG Labtech).
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bioRxiv - Cell Biology 2022Quote: ... Cell pellet was resuspended in 2 mL Red blood cell lysis buffer (Roche) with 5 µL DNAse (1 mg/mL) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell pellets were resuspended in red blood cell lysis buffer (Roche, cat.# 11814389001) and incubated for 1 min at RT ...
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Senescence-Induced Immune Remodeling Facilitates Metastatic Adrenal Cancer in a Sex-Dimorphic MannerbioRxiv - Cancer Biology 2022Quote: ... Red blood cells were removed using Red Blood Cell Lysis buffer (Roche, 11814389001) according to manufacturer guidelines and the cells were washed twice in HBSS containing 2% FBS before counting on a Countess Automated Cell Counter (Thermo Fisher).
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bioRxiv - Immunology 2019Quote: ... Single-cell suspensions of lung cells were prepared by Liberase Blendzyme 3 (Roche) digestion of perfused lungs as previously described48 ...
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bioRxiv - Cancer Biology 2019Quote: ... The spleen cells were fused with P3U1 cells (ATCC) using PEG1500 (Roche Diagnostics,). The hybridomas were grown in RPMI 1640 medium (Nacalai Tesque ...
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bioRxiv - Biochemistry 2021Quote: ... cell viability was measured using the Cell Proliferation Kit I (Roche, Basel, Switzerland) in a 3-fold determination ...
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bioRxiv - Developmental Biology 2021Quote: ... To detect cell death the “In Situ Cell Death AP kit” (Roche, #11684809910) was used ...
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bioRxiv - Immunology 2020Quote: ... Cell numbers and viability were determined using a CASY TT Cell Counter (Roche). 2×106 cells were transferred to 96-well plates for antibody staining ...
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bioRxiv - Immunology 2023Quote: ... Cell numbers and viability were determined using a CASY TT Cell Counter (Roche). A small aliquot of enriched B cells was taken and stained to determine the percentage of NP-binding B cells by flow cytometry before cell transfer ...
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bioRxiv - Immunology 2023Quote: ... Cell numbers and viability were determined using a CASY TT Cell Counter (Roche). Cells were washed and transferred into 96-well v-bottom plates ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were lysed in Cell Death Detection Lysis Buffer (Roche, Basel, Switzerland) for analysis of insulin content ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured using the Cell Proliferation Kit I (MTT) (Roche, #11465007001) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The apoptotic cells were defined by using In Situ Cell Death Detection Kit (Roche) following manufacturer’s protocol and quantified by calculating positively stained cells in at least five randomly chosen HPFs of each slide.
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bioRxiv - Neuroscience 2019Quote: Cells were lysed using Real-Time ready cell lysis buffer (Roche Life Science, 7248431001) with 1:80 diluted RNAse inhibitor (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Apoptotic cells were quantified by the In situ cell death detection kit (12156792910, Roche) according to the manufacturer’s instructions ...