Labshake search
Citations for Roche :
401 - 450 of 1277 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: Plasmids were obtained from Transomic Technologies and the antisense mRNAs were synthesized using the Digoxigenin RNA Labeling Kit (SP6/T7; Roche, 11175025910). DIG RNA in situ hybridization (ISH ...
-
bioRxiv - Cell Biology 2023Quote: ... libraries were prepared in an automated fashion using an Agilent Bravo robot with a KAPA Standard mRNA-Seq Kit (KAPA BIOSYSTEMS). In house adaptors were ligated to 100-300 bp fragments of dsDNA ...
-
bioRxiv - Genomics 2024Quote: ... mRNA was then isolated and RNA-Seq library was prepared following the KAPA RNA HyperPrep Kit protocol (Roche, Cat. No: 08098123702). The RNA- seq libraies were sequenced by NovaSeq 6000 (Illumina ...
-
bioRxiv - Developmental Biology 2024Quote: ... RNA sequencing (RNA-seq) libraries were prepared from 4 µg of total RNA using the KAPA stranded mRNA-seq kit (Roche, KK8421) according to manufacturer’s specifications ...
-
bioRxiv - Developmental Biology 2019Quote: ... Sample preparation protocol for the RNASeq libraries was following the manufacturer’s recommendations of KAPA Stranded mRNA-Seq Illumina® Platforms Kit (Roche-Kapa Biosystems). The libraries were sequenced on HiSeq2500 (Illumina ...
-
bioRxiv - Neuroscience 2020Quote: Expression of UPR marker and CDNF mRNAs was quantified using the LightCycler® 480 Real-Time PCR System and Lightcycler 480 SYBR Green I master mix (Roche, Switzerland). β-actin was chosen as a reference gene ...
-
bioRxiv - Neuroscience 2019Quote: ... and was used to prepare libraries for RNA sequencing using the KAPA mRNA HyperPrep Kit according to the manufacturer’s instructions (KAPA Biosystems, Wilmington, MA). Libraries were quality control checked via Qubit (ThermoFisher ...
-
bioRxiv - Immunology 2020Quote: ... Total RNA was then provided to the Functional Genomics Laboratory at UC Berkeley where RNA-seq libraries were prepared by Oligo dT enrichment followed by a stranded Illumina library prep protocol with the KAPA mRNA HyperPrep kit (Kapa Biosystems, KK8580). Libraries were checked for quality on an AATI Fragment analyzer (Agilent ...
-
bioRxiv - Genomics 2020Quote: ... Whole transcriptome libraries were constructed using the KAPA Stranded mRNA-seq library kit (Roche Sequencing and Life Science, Kapa Biosystems, Wilmington, MA) from 250ng of total input RNA with the Beckman Coulter Biomek FXP automation system (Beckman Coulter ...
-
bioRxiv - Cancer Biology 2023Quote: ... Strand-specific and barcode-indexed RNA-Seq libraries were generated from 300 ng total RNA each after poly-A enrichment using the mRNA-Seq Hyper Kit (Kapa Biosystems, KK8581) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... A DNA library targe9ng polyadenylated transcripts was constructed for each sample (100ng total RNA) with the KAPA hyper mRNA stranded library prep kit (Roche, catalogue# KK8581) in a 12-cycle PCR ...
-
bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA stranded mRNA kit (v4.16) (Kapa Biosystems, Wilmington, MA). RNA was sheared to 300-400 bp ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... RNAseq libraries were prepared in a single batch by the Bauer Core at Harvard using the KAPA mRNA HyperPrep Kit (Roche, Palo Alto, CA) with 300-500 nanograms of input RNA ...
-
bioRxiv - Genomics 2020Quote: ... cDNA libraries were prepared using 500 ng of large RNA as input for the KAPA mRNA HyperPrep Kit (Kapa Biosystems; Wilmington, MA, USA) combined with the KAPA Unique Dual-Indexed Adapter Kit (Kapa Biosystems ...
-
bioRxiv - Immunology 2022Quote: ... Library for RNA-Seq was prepared according to KAPA Stranded mRNA-Seq poly(A) selected kit with 201-300bp insert size (KAPA Biosystems, Wilmington, MA) using 250 ng total RNAs as input ...
-
bioRxiv - Cancer Biology 2022Quote: A total amount of 1 μg total RNA per sample was used for sequencing libraries generation by using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
-
bioRxiv - Cancer Biology 2019Quote: Four RNA-seq libraries (from CER217, CER218, CER220, and CER221 strains) were prepared with the KAPA Stranded mRNA-Seq Illumina® Platforms Kit (Roche-Kapa Biosystems) following the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... 500 μg total RNA was used to prepare polyA-enriched RNA-seq libraries using the KAPA mRNA Hyper Prep Kit (Roche, ref. KK8581/KK8581). Those libraries were prepared separately for each sample with 11 amplification cycles ...
-
bioRxiv - Microbiology 2022Quote: ... #439259) or HeV (Advanced Cell Diagnostics Inc #410719) and detected using the Discovery mRNA purple HRP detection kit (Roche Tissue Diagnostics #760-255) on the Ventana Discovery ULTRA staining platform (Roche Tissue Diagnostics) ...
-
bioRxiv - Physiology 2023Quote: ... Library for RNA-Seq was prepared according to KAPA Stranded mRNA-Seq poly(A) selected kit with 201-300bp insert size (KAPA Biosystems, Wilmington, MA) using 250 ng total RNA as input ...
-
bioRxiv - Developmental Biology 2023Quote: ... Libraries were prepared by the Van Andel Genomics Core from 1ug of total RNA using the KAPA stranded mRNA kit (v5.16) (Kapa Biosystems, Wilmington, MA USA). RNA was sheared to 300-400 bp ...
-
bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA mRNA Hyperprep kit (v4.17) (Kapa Biosystems, Wilmington, MA USA). RNA was sheared to 300-400 bp ...
-
bioRxiv - Genetics 2023Quote: ... RNA sequencing libraries were prepared as described previously (Ma, Fuqua et al. 2019) using the KAPA Stranded mRNA-Seq Kit (cat #KK8421, KAPA Biosystems, Wilmington, MA). The pooled libraries were sequenced in an Illumina HiSeq4000 instrument (Illumina ...
-
bioRxiv - Cancer Biology 2023Quote: Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA mRNA Hyperprep kit (v4.17) (Kapa Biosystems, Wilmington, MA USA). RNA was sheared to 300-400 bp ...
-
bioRxiv - Cancer Biology 2020Quote: ... cell lysates were prepared as described previously [17] Quantitative detection of mRNA was performed by real-time PCR using the Lightcycler 480 detector (Roche Applied Science, Manheim Germany) as previously published [17].
-
bioRxiv - Cancer Biology 2022Quote: A total amount of 1 μg total RNA per sample was used for sequencing libraries generation by using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
-
bioRxiv - Cell Biology 2020Quote: ... The RNA-Seq Library was prepared according to KAPA Stranded mRNA-Seq poly(A) selected kits with 201-300 bp insert sizes (KAPA Biosystems, Wilmington, MA, USA) using 250 ng total RNA as an input ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... n=40) and brain (n=40) for each individual using the KAPA mRNA HyperPrep Kit and Unique Dual-Indexed Adapters (KAPA Biosystems; Wilmington, MA, USA), with 100 ng of large RNA as input ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... first purified with two rounds of polyA mRNA selection with oligo-dT magnetic beads and then converted to sequencing-compatible cDNA using the KAPA mRNA HyperPrep kit (KAPA biosystems, Cat. No. KK8580), using the KAPA single-indexed adapter kit for multiplexing (KAPA biosystems ...
-
bioRxiv - Zoology 2023Quote: ... Paired-end cDNA libraries were constructed from 4 μg of total RNA using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, Inc., Wilmington, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... Stimulated cultures were lysed in M-PER mammalian protein extraction reagent supplemented with PhosphoSTOP (Roche) and cOmplete Tablets (Roche ...
-
bioRxiv - Cell Biology 2022Quote: ... 3500 nucleotides located upstream of the transcription initiation of FN mRNA were amplify from HUVEC genomic DNA using KAPA HiFi HotStart (Kapa Biosystems, Wilmington, MA, USA, #07958889001) and cloned upstream of the luciferase coding sequence of the pGL3-Basic plasmid (Promega ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The protein extraction was performed by adding 10 M urea containing complete protease inhibitor cocktail (Roche) and Halt™ Phosphatase Inhibitor (Thermo ...
-
bioRxiv - Cell Biology 2023Quote: ... HBE were processed for protein lysates and stored at -20°C (Complete Lysis-M Roche diagnostics). NET dosage was an average of extracellular dsDNA concentration (QuantiFluor ONE dsDNA System ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... mRNA was purified from approximately 200 ng of total RNA with oligo-dT beads and sheared by incubation at 94 C in the presence of Mg (Roche Kapa mRNA Hyper Prep Catalog # KR1352).
-
bioRxiv - Plant Biology 2023Quote: ... and 1.5 ug RNA was used for library preparation at the High-throughput Sequencing Facility at UNC Chapel Hill using the Stranded mRNA-Seq kit (Kapa Biosystems. The NovaSeq 6000 sequencer (Illumina) generated 50bp paired-end reads with a read depth of 23-35 million reads per biological replicate ...
-
bioRxiv - Cell Biology 2022Quote: ... Mouse monoclonal antibodies were used to detect green fluorescent protein (GFP) (Cat#11814460001, clones 7.1 and 13.1, Roche). Rabbit polyclonal anti-Cdc42p antibodies were provided by Dr ...
-
bioRxiv - Cell Biology 2020Quote: Cells were lysed and proteins were purified by Complete™ Lysis-M EDTA-free kit (Roche, 04719964001). Protein samples were run on Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad ...
-
bioRxiv - Physiology 2023Quote: ... cells/tissue were lysed in mammalian protein extraction reagent (M-PER) supplemented with protease inhibitor cocktail (Roche). Soluble proteins and insoluble matrix were separated by centrifugation at 10,000 × g ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were lysed in 8 M urea buffer (8 M urea, 1 M Tris pH 7.4, 5 M NaCl) containing protease and phosphatase inhibitors (Roche) followed by sonication at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were incubated in staining solution (1 M Tris-HCl pH 9.5, 5 M NaCl, 1 M MgCl2, NBT-BCIP, H2O) (Roche) in the dark for 10 min at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... with fluorescent standards (KAPA Biosystems). Libraries were sequenced twice on the Illumina Nextseq platform (Illumina ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-Green Fluorescent Protein overnight at 4°C (Clones 7.1 and 13.1; 1:50 dilution, Roche, Mannheim, Germany) and rat monoclonal anti-HA High Affinity for 60 minutes at room temperature (clone 3F10 ...
-
bioRxiv - Immunology 2021Quote: Cells were collected in RIPA buffer (0.5 M EDTA, 1 M Tris-Cl pH7.5, 1 M NaCl, 200 mM, Roche protease inhibitor) at 0h ...
-
bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The coding region of the sequence (flanked by sequences specific to T7 (Roche T7 RNA polymerase Sigma cat no 1088176700 ...
-
bioRxiv - Neuroscience 2020Quote: ... or anti-fluorescent antibodies (#11207733910, Roche) overnight at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... or anti-fluorescent antibodies (#11207733910, Roche) overnight at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... MSNs were harvested in M-PER Mammalian Protein Extraction Reagent (Pierce) with cOmplete Mini EDTA-free protease inhibitor (1 tablet/10 mL) (Roche). Whole-cell lysates were sonicated with a 5 second pulse followed by a 5 second rest (X 5 times ...
-
bioRxiv - Cancer Biology 2020Quote: Protein extracts were prepared from drug-treated cells by lysis on ice for 20 minutes in M-PER Mammalian Protein Extraction Reagent (ThermoScientific) supplemented with protease and phosphatase inhibitors (Roche). Lysates were centrifuged at 16,000 RPM for 20 minutes at 4°C and the supernatants were assessed for concentration via a Bradford assay ...