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Citations for Roche :
451 - 500 of 1277 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Western blot analysis of infected and uninfected erythrocytic material performed on protein extracts prepared by resuspending cells in M-PER mammalian protein extraction reagent (Pierce) supplemented with 1x protease inhibitory cocktail (EDTA free) (Roche), 300 mM benzamidine ...
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bioRxiv - Genomics 2022Quote: ... fourteen non-coding regions of interest were PCR amplified from human genomic DNA (Roche) using the Phusion High-Fidelity PCR Kit (NEB) ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in M-PER mammalian protein extraction reagent (Thermo, MA, USA) with protease and phosphatase inhibitors (Roche, Basel, Switzerland), and proteins were quantified using protein assays (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 48 h at 37°C in 5% CO2, harvested and lysed using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) (with 1X Roche Complete Protease Inhibitor and 100 mM NaCl) ...
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bioRxiv - Cell Biology 2021Quote: Total protein from cells or mouse tissues (n=3 per genotype) were extracted using the M-PER protein lysis buffer (ThermoScientific, Beverly, MA) containing protease inhibitors (Roche, Indianapolis, IN). Approximately 25 μg of total protein was electrophoresed on 4-12% SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and lysed (200 µl) with M-PER Mammalian Protein (ThermoScientific®) plus 1x Protease inhibitor (cOmplete Mini EDTA, Roche®). Cells were detached using a scraper (on ice) ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the gene-coding sequences via in vitro transcription using DIG RNA Labeling Kit (Roche, 11175025910). A short bleaching was used to remove the pigments from the fixed zebrafish embryos.
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bioRxiv - Cancer Biology 2019Quote: ... together with Rhodamine and FAM fluorescent substrates (Roche/Ventana ...
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bioRxiv - Neuroscience 2020Quote: ... Forebrains were finely chopped and then homogenized in 0.32 M sucrose solution (0.32 M sucrose, 10 mM Hepes pH 7.4, with cOmplete protease (Roche) and phosphatase inhibitors ...
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bioRxiv - Bioengineering 2023Quote: ... 3 % NaCl (5 M, v/v), 0.08 % CaCl2 (2.5 M, v/v) and cOmpleteTM ULTRA Protease Inhibitor Cocktail tablets (Roche) in H2O ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 M EDTA and Protease Inhibitor (Roche). The cell lysate was collected by centrifuging the homogenate at 20,000g for 10 min ...
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bioRxiv - Immunology 2023Quote: ... for GFP and mCherry or 1 mg/mL G-418 solution (Roche, 04727878001) for luciferase ...
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bioRxiv - Genetics 2020Quote: ... Cells pellets were combined and lysed in a denaturing urea buffer (8 M urea, 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1.5 ml 5 M NaCl, 12.5 μL 2 M Spermidine, final volume brought to 50 ml with dH2O, complemented with one Roche Complete Protease Inhibitor EDTA-Free tablet) ...
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bioRxiv - Molecular Biology 2024Quote: ... The remaining supernatant was diluted in 1 mL pre-warmed capturing buffer (0.5 M LiCl, 4 M urea, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and 10 µL from an equimolar stock (10 µM ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting supernatant is the soluble fraction and the insoluble fraction is generated by resuspending the pellet in urea buffer (30 mM Tris, 7 M Urea, 2 M Thiourea, 4% CHAPS, 1X Protease Inhibitor Cocktail (Roche), 0.5 mM PMSF ...
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bioRxiv - Neuroscience 2022Quote: ... Cell pellets were homogenized in lysis buffer (7 M urea, 2 M thiourea, 1% CHAPS, 70 mM DTT, and Roche EDTA-free complete protease inhibitor cocktail ...
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bioRxiv - Biochemistry 2021Quote: ... 8 M urea and protease inhibitor (Roche, 05892791001) with sonication on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.05 M TRIS-HCl (Roche Cat. No. 10812846001), 0.8 mM EDTA (Sigma Aldrich Cat No ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.25 M KCl) supplemented with protease inhibitors (Roche) and sonicated 3x5 seconds using a Bioruptor (Diagenode) ...
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bioRxiv - Neuroscience 2022Quote: ... and DAPI fluorescent die (1:300, Roche Diagnostics, Indianapolis, IN) were incubated at RT for 1 h ...
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bioRxiv - Plant Biology 2023Quote: ... 0.2 mM EDTA, 0.3 M NaCl, 1 M Urea, 1% IGEPAL CA-630, 1 mM PMSF, and Roche protease inhibitor cocktail) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... was introduced by site directed mutagenesis on C-terminal SNAP-tagged H3.1 (HIST1H3C coding sequence cloned in pSNAPm) and H3.3 (H3F3B coding sequence cloned in pSNAPm) encoding plasmids with a PCR master kit (Roche) and the primers indicated in Supplementary table 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Initial T cell activation was performed with anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10µg/ml Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentivirus transduction procedure ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were pelleted and resuspended in 2× SDS-PAGE solvent (4% SDS, 20% glycerol, 0.1 M DTT, 0.125 M Tris-HCl pH 7.5 and Roche EDTA-free protease inhibitor cocktail) and boiled at 95°C for 5min ...
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bioRxiv - Biophysics 2020Quote: ... EDTA-free lysis-M buffer with protease inhibitor (Roche). Protein concentrations were determined using PierceTM BCA kit (Thermo ScientififTM) ...
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bioRxiv - Neuroscience 2021Quote: ... 0.15 M NaCl and Complete protease inhibitor cocktail (Roche)] ...
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bioRxiv - Immunology 2021Quote: ... hydrocortisone hemisuccinate (7×10−5 M, Roche-Boehringer-Manheim) and FCS (10% selected ...
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bioRxiv - Neuroscience 2019Quote: ... 0.15 M NaCl and complete protease inhibitor cocktail (Roche) by ultrasonication ...
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bioRxiv - Cell Biology 2023Quote: ... 0.3 M sucrose containing Complete Protease Inhibitor Cocktail (Roche). Suspended cells were homogenized using a syringe with a 27-gauge needle ...
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Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosisbioRxiv - Evolutionary Biology 2019Quote: ... Rec8 and Scc1 were all tagged with 3xHA at the C-terminus of coding sequences and anti-HA antibody (3F10, Roche) was used to detect the abundance of kleisin proteins ...
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bioRxiv - Immunology 2023Quote: ... Initial T cell activation was performed using anti-CD3/anti-CD28 microbeads (T Cell TransAct, Miltenyi Biotech, 130-111-160) and 10 μg/mL Phytohemagglutinin-M (PHA-M) (Roche 11082132001, 20 mg). Lentiviral transduction was performed by inoculating cells with Vectofusin 1 (10 μg/mL ...
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bioRxiv - Microbiology 2019Quote: ... Quantification of viral RNA was performed using a real-time RT-PCR assay specific for HTNV nucleocapsid coding region in a LightCycler® 96 (Roche) following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: An antisense RNA DIG-probe was generated by transcription from linearized pCS1 vector containing Sorbs1 coding sequence using SP6 RNA polymerase kit where UTPs were labelled with digoxigenin (DIG) (Roche, 11175025910).
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bioRxiv - Developmental Biology 2023Quote: ... labelled RNA probes were achieved by reverse transcription and purification of the amplified partial coding sequence of selected genes with a DIG RNA labelling kit (Roche, Switzerland). For 75 and 150 hpf larvae ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 M β-mercaptoethanol) supplemented with protease inhibitor cocktail (Roche) and sonicated for 10 s at 20% amplitude using a Sonics Vibra Cell sonicator fitted with a microprobe ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 M sucrose) supplemented with one protease inhibitor tablet (Roche). Osmotic shock was performed by the addition of diluted TES buffer to release the periplasmic proteins ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 M sucrose) supplemented with one protease inhibitor tablet (Roche). Osmotic shock was performed by the addition of diluted TES buffer to release the periplasmic proteins ...
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bioRxiv - Biophysics 2022Quote: ... 0.02% NaN3 and 1x cOmpleteT M Protease Inhibitor Cocktail (Roche). 10% D2O was added for lock.
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bioRxiv - Microbiology 2021Quote: ... 2 M CaCl2 and 2 UU/ml DNAse I (Roche) for 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... and suspended in 8 M urea containing PhosSTOP (Roche Diagnostics) and Benzonase (Novagen) ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 M β-mercaptoethanol) supplemented with protease inhibitor cocktail (Roche) and lysates were sonicated for 10 s at 20% amplitude using a Vibra-Cell sonicator (Sonics ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Digoxigenin (DIG)- and biotin-labelled antisense probes of target IRs were transcribed from linearized pCS2+ vectors with the coding region of the corresponding receptors using the T7 RNA transcription kit (Roche, Mannheim, Germany). Labelled probes were subsequently fragmented to an average length of about 800 bp by incubation in sodium carbonate buffer (80 mM NaHCO3 ...
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bioRxiv - Molecular Biology 2023Quote: ... CFP/GFP and mCherry probes were synthesized using the PCR digoxigenin probe synthesis kit (1163090910, Roche, Switzerland) with primers shown in Table 1 and the following PCR conditions ...
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bioRxiv - Physiology 2024Quote: ... and incubated in the staining buffer containing VHH15-mCherry (1:30 dilution) and protease inhibitor cocktail (Roche) in Krebs buffer for 2 hours at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... At the end of the cell disruption dry homogenised powder of cells were resuspended in 1 ml urea buffer (8 M urea, 50 mM Tris/HCl pH 8.0 containing one tablet of ‘cOmplete’ protease inhibitor per 25 ml, Roche, Germany; 1 mM DTT; 0.1 M PMSF) and centrifuged at 16,000 × g for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 M NaCl with Complete protease inhibitor tablet (Roche, Indianapolis, IN) and centrifuged for 30 min at 13,000 rpm 4°C to pellet cell debris ...
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bioRxiv - Neuroscience 2021Quote: ... 0.32 M sucrose supplemented with Complete mini protease inhibitors (# 11836153001, Roche), PhosStop phosphatase inhibitor cocktail (# 4906837001 ...
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bioRxiv - Neuroscience 2020Quote: ... 0.25 M sucrose and EDTA-free protease inhibitors (Roche, cat# 05056489001) followed by centrifugation at 9000 rpm for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates were prepared using cOmplete™ Lysis-M (4719956001, Roche) with protease and phosphatase inhibitors and subjected to standard electrophoresis and transfer as previously described41 ...