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Citations for Roche :
451 - 500 of 1612 citations for IL 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 5 μl of KAPA HiFi 2X master mix (Roche) and 3 μl of diluted overnight culture grown in 96-well plates ...
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bioRxiv - Immunology 2024Quote: ... 5% glycerol) supplemented with protease inhibitors (Complete mini, Roche), and immunoprecipitation was performed with 2μg of anti-survivin antibodies (RnD AF886 ...
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bioRxiv - Immunology 2020Quote: ... NK cells were then lysed in lysis buffer (IP Lysis Buffer, Pierce Biotechnology Inc., Rockford, IL) supplemented with protease inhibitors (Complete Mini, EDTA-free Cocktail tablets, Roche Holding, Basel, Switzerland) and incubated for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Proliferating cells remaining in the cultures were further expanded by incubating for a further 7 days with 20 IU/mL IL-2 (Roche Life Science Products). After expansion ...
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bioRxiv - Cancer Biology 2019Quote: ... Pellets were resuspended in 5 mL of membrane isolation buffer (5 mM TrisHCl pH 7.6, 5 mM MgCl2, 1 mM EGTA, protease inhibitor cocktail cOmplete [Roche Applied Science, Penzberg, Germany]) with a glass dounce tissue grinder (Wheaton ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgOAc, 5 %w/v glycerol, 0.25 %w/v NP-40, 3 mM DTT, 1 mM PMSF, Roche protease inhibitor cocktail (PIC), pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... pellets were resuspended in lysis buffer (50 mM HEPES pH 8, 400 mM NaCl, 75 mM Imidazole pH 8, 5% v/v glycerol, 5 mM 2-Mercaptoethanol, supplemented with Roche Protease Inhibitor Tablets) and lysed using an Emulsiflex-05 homogenizer ...
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bioRxiv - Molecular Biology 2019Quote: ... washed once with ice cold Phy Buffer (150 mM Potassium Acetate, 5 mM Magnesium Acetate, 20 mM HEPES-KOH pH 7.4, 5 mM DTT, protease inhibitor cocktail [Roche], 10 µg/mL cycloheximide). Cell pellets were then re-suspended in 1 mL Phy Buffer ...
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Tryptophan Metabolites And Their Predicted Microbial Sources In Fecal Samples Of Healthy IndividualsbioRxiv - Microbiology 2024Quote: ... Libraries were amplified for 13 PCR cycles in 50 μl reactions containing 150 pmol of P1.1 (5’-AATGATACGGCGACCACCGAGA) and P3 (5’-CAAGCAGAAGACGGCATACGAGA) primer and Kapa HiFi HotStart Library Amplification kit (Cat# kk2612, Roche Sequencing and Life Science). Library quantification and size estimation were performed using a Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... 30 ml of additional human dynein lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche) was added to the frozen cell pellet ...
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bioRxiv - Neuroscience 2020Quote: ... The RNA probes complementary to human METTL7B cDNA (NM_152637.2) were labeled with digoxigenin-UTP (Roche). After acetylation ...
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bioRxiv - Genetics 2022Quote: ... Probes were then mixed with 20μg of COT Human DNA (Roche, 11 581 074 001) and 79μg of Salmon sperm DNA (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... Human periocular skin was digested in 2.4 U/ml dispase type II (Roche, Basel, Switzerland) at 4 °C for 12 hours ...
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bioRxiv - Cell Biology 2022Quote: ... 30 ml of additional human dynein lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche) was added to the frozen cell pellet ...
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bioRxiv - Cell Biology 2019Quote: ... 30 ml of additional human dynein lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche) was added to the frozen cell pellet ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and ethanol precipitated with human Cot-1 DNA (Roche, Germany) and herring sperm DNA (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: Recombinant human (rh)EPO (5000 IU/kg body weight; NeoRecormon, Roche, Welwyn Garden City, UK) or placebo (PL ...
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bioRxiv - Cancer Biology 2023Quote: ... and retrotranscribed cDNA quantified using the Universal Human Probe Roche library (Roche-Diagnostics, Barcelona, Spain). Assays were made in triplicate and normalized to TBP expression (ΔΔCT method) ...
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bioRxiv - Biophysics 2022Quote: ... The PVDF membranes were developed using an enhanced chemiluminescence western blot detection kit (Pierce SuperSignal® West Dura, Rockford, IL) and exposed to Lumi-Film chemiluminescent detection films (Roche Diagnostics, Mannheim, Germany). The experiment was replicated five times ...
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bioRxiv - Plant Biology 2019Quote: ... 300 mM sucrose, 1% Triton X-100) containing 1x protease inhibitor cocktail (Pierce, Rockford, IL) and a phosphatase inhibitor (PhosSTOP, Roche Applied Science, Penzberg, Germany). Protein extracts were centrifuged at 1,500 rpm for 5 min and the supernatant was re-centrifuged at 12,000 rpm for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 20 uL/mL Liberase TL (5 mg/ml, Roche) and 50 U/ml DNAse I (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate (BCIP, Roche).
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bioRxiv - Molecular Biology 2021Quote: ... followed by addition of pefabloc (Roche, 5 mM final concentration) and then dialyzed into 10 mM Tris pH 7.5 with 2 mM MgCl2 ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by addition of pefabloc (Roche, 5 mM final concentration), then dialyzed into 10 mM tris(hydroxymethyl)aminomethane (Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% glycerol) containing 1×EDTA-free protease inhibitor cocktail (Roche), sonicated and centrifuged at 10,000 × g for 5 min twice ...
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bioRxiv - Immunology 2021Quote: ... containing 20 uL/mL Liberase TL (5 mg/ml, Roche) and 50 U/ml DNAse I (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... and 5 mg/mL eComplete Mini protease inhibitor tablet (Roche). Lysates were sonicated for 10 seconds 8 times in an ice-cold water bath and centrifuged to separate the protein extract ...
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bioRxiv - Cell Biology 2019Quote: ... 5% glycerol) plus cOmplete protease inhibitor cocktail without EDTA (Roche). The suspension was clarified by a series of consecutive centrifugations (500 × g for 10 min ...
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bioRxiv - Neuroscience 2019Quote: ... 5 μl of KAPA2G Fast HotStart ReadyMix (KK5603, KAPA Biosystems) and 3.5 μl of sterile water were mixed in a 10 µl reaction ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl RNase A (2.5 g, DNase-free, Roche #11119915001) or dH2O ...
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bioRxiv - Immunology 2019Quote: ... for 3 h followed by 5 mM ATP (10519987001, Roche) or 20 μM nigericin (N-7143 ...
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bioRxiv - Genetics 2020Quote: ... 5 µL of 2x Kapa library amplification mix (Roche, KK2702), 0.2 µL forward primer (10 µM ...
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bioRxiv - Neuroscience 2020Quote: ... and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP, Roche) in NTMT pH9.5 solution (100mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... 5-bromo-4-chloro-3-indolyl-phosphate (BCIP, #11383221001, Roche) and 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride (INT ...
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bioRxiv - Biochemistry 2020Quote: ... 5 U/mL rabbit pyruvate kinase (Roche Diagnostics, Cat# 10128155001), 8 U/mL lactate dehydrogenase (Sigma-Aldrich)) ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM BME) including cOmplete EDTA-free protease inhibitor (Roche) and subjected to lysis by sonication ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM BME) including cOmplete EDTA-free protease inhibitor (Roche) and subjected to lysis by sonication ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mg DNase I (from Bovine Pancreas grade II, Roche) was added to 50 mL of lysate ...
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bioRxiv - Microbiology 2020Quote: ... 5% Na deoxycholate) supplemented with protease/phosphatase inhibitor cocktail (Roche) and clarified from the nuclei by centrifugation at 13,000×g for 10 min at 4°C for quantitative western blot analysis (see below) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% glycerol and mini-complete EDTA-free protease inhibitor (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM β-mercaptoethanol supplemented with protease inhibitor cocktail (Roche). Cell lysis was accomplished by sonication ...
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bioRxiv - Cell Biology 2021Quote: ... 5% glycerol and protease inhibitors (Complete Protease Inhibitor Cocktail, Roche). The lysate was centrifuged at 160,000 x g for 20 min ...
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bioRxiv - Cancer Biology 2021Quote: ... phosSTOP phosphatase inhibitor mixture (0.5 tablet for 5 mL, Roche) and 1% n-octylglucoside in 50 mM ammonium bicarbonate (ABC ...
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bioRxiv - Cell Biology 2022Quote: ... 5% glycerol) supplemented with EDTA-free cOmplete protease inhibitor (Roche). Samples were incubated on ice with intermittent agitation by pipetting for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol] supplemented with EDTA-free protease inhibitor cocktail (Roche), sonicated ...
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bioRxiv - Neuroscience 2021Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate (Roche Diagnostics) in detection buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... together with 5 µl of a 1kB DNA Ladder (Roche). The electrophoresis was performed at 90 V constant.
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bioRxiv - Microbiology 2021Quote: ... 5% glycerol) with cOmplete EDTA-free protease inhibitor cocktail (Roche) and disrupted by sonication for 3 min (10 s ON ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and midazolam (5 mg/ml; Hoffmann - La Roche, Hvidovre, Denmark), respectively ...
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bioRxiv - Microbiology 2019Quote: ... albumin (5 g/L; fraction V, fatty acid-free, Roche) and tyloxapol (0.04%) ...