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Citations for Roche :
251 - 300 of 557 citations for Cytokeratin 8 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-GFP (1:1000; 11814460001, Roche). Secondary antibodies used were HRP-conjugated ...
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bioRxiv - Cancer Biology 2021Quote: ... PD-L1 (rabbit monoclonal, clone SP142, Roche) and CD163 (mouse monoclonal ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-EGFR (prediluted, Roche, 790-4347), rabbit anti-TFF3 (1:250 ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by HRP-conjugated anti-rabbit (Roche) or anti-mouse (Santa Cruz ...
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bioRxiv - Developmental Biology 2021Quote: Digoxigenin-labelled sense and antisense RNA probes were prepared by in vitro transcriptions using recombinant plasmids of target genes made as mentioned above (Roche Life Science) and used for in situ hybridization ...
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bioRxiv - Neuroscience 2024Quote: ... brain slices were permeabilized by 0.3% triton in PBS and positive control slices were incubated with Dnase I recombinant (Roche, Basel, Switzerland, #4536282001) (1,000 units) ...
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bioRxiv - Developmental Biology 2024Quote: ... The pIEx-4-BmU6-Cad96ca-gRNA-Cas9-GFP-P2A-Puro or pIEx-4-BmU6-Fgfr1-gRNA-Cas9-GFP-P2A-Puro recombinant vectors were transfected into HaEpi cells with transfection reagent (Roche, Basel, Switzerland). After 48 h of vector transfection (cells can be observed to express green fluorescent protein) ...
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bioRxiv - Molecular Biology 2021Quote: ... Final DNA concentration of 1μM and increasing concentrations of Stl (0.5-8 μM) were mixed with 1μg/ml poly(d[I-C]) (Roche) in EMSA buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Neuroscience 2020Quote: ... brain from transgenic mice expressing different PrP deletion mutants was homogenized in 10 volumes of lysis buffer (50 mM Tris-HCl pH 8, 0.5% Na deoxycholate, and 0.5% Igepal, protease inhibitors (complete Mini, Roche) using TissueLyser LT for 5 min for 2 cycles ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in 450 μl lysis buffer (20 mM Tris pH 7.5, 0.5 mM EDTA pH 8, 200 mM NaCl, 10% glycerol, 1 mM PMSF, 10 mM NEM, Roche cOmplete protease inhibitor catalog # 11836153001) ...
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bioRxiv - Microbiology 2020Quote: RNA of high quality (RINe>8) was prepared for mRNA-sequencing using the KAPA mRNA HyperPrep kit (Roche). Libraries were quantified using a High Sensitivity D1000 ScreenTape (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... and chloride [Cl-] concentrations were determined using an Ion Selective Electrode (Hitachi Modular P+ISE. Roche 8 Diagnostic). Plasma osmolality was analyzed by vapor pressure osmometry (VAPRO 5520 ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1 × protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 μl/10 mg) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1× protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 µl/10 mg) ...
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bioRxiv - Molecular Biology 2021Quote: Protein lysates were prepared from snap-frozen dorsal skin with urea buffer (8 M Urea, 40 mM Tris-HCl, 2.5 mM EDTA, pH 8.0) containing phosphatase (PhosSTOP, Roche) and protease inhibitors (cOmplete Protease Inhibitor Cocktail Tablet EDTA-free ...
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bioRxiv - Cell Biology 2020Quote: ... This was followed by 8 min of perfusion with digestion buffer (4mg/mL trypsin, 5mg/mL liberase (Roche) and 0.3mM CaCl2) ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were lysed with Cell Lysis Buffer (10mM Tris-Cl pH 8, 10mM NaCl, 0.5% NP40, 1xPIC (Roche)) followed by mechanical shearing for 10minutes on ice ...
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bioRxiv - Molecular Biology 2022Quote: Pelleted cells were lysed in 500 μl 8 M urea (pH 8.0) with 0.2% Rapidgest (Waters) and protease inhibitors (Roche) using three 20-s sonication pulses in a VialTweeter (DrHielscher) ...
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bioRxiv - Cell Biology 2022Quote: ... the whole-cell extracts and immunoprecipitates were subjected to western blotting using monoclonal anti-GFP (JL-8, Roche), Flag-M2 monoclonal antibody (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: Cell lysates were harvested at indicated time points with RIPA buffer (50mM Tris pH 8, 150mM NaCl, 0.5% deoxycholate, 0.1% SDS, 1% NP40) supplemented with protease inhibitors (Roche: cOmplete mini EDTA-free protease inhibitor ...
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bioRxiv - Plant Biology 2022Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1× protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 µl/10 mg) ...
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LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicrybioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 20 mM Tris-HCl pH 8 containing a EDTA-free protease inhibitor cocktail (Roche). For the purification of protein samples that had to be analyzed both by reducing and non-reducing SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... tissue slides were heat pre-treated using a Cell Conditioning Buffer 1 (pH 8) (Roche Diagnostic, Meylan, France) at 98°C ...
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bioRxiv - Microbiology 2023Quote: ... pH 8) supplemented with 0.2 mg/mL Lysozyme and EDTA free protease inhibitor cocktail (cOmplete, Roche, Basel, Switzerland) and incubated for 30 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... and with palindromic forked adapters using unique 8-base index sequences embedded within the adapter (purchased from Roche). The libraries are then amplified by 10 cycles of PCR ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were harvested at indicated time points using RIPA buffer (50mM Tris pH 8, 150mM NaCl, 0.5% deoxycholate, 0.1% SDS, 1% NP40) supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with ice-cold PBS two times and lysed in 300 μl RIPA buffer (50 mM TRIS pH 8, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, Roche Complete protease inhibitors ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with ice-cold PBS two times and lysed in 300 μl RIPA buffer (50 mM TRIS pH 8, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, Roche Complete protease inhibitors ...
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bioRxiv - Neuroscience 2022Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... sections were fixed with 4% PFA (PierceTM, 28908) in PBS for 15 minutes at room temperature and permeabilized with PCR grade recombinant Proteinase K (Roche Applied Science, 3115887001) for 30 minutes at 37 °C ...
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bioRxiv - Pathology 2019Quote: ... Ventana anti-Her2/neu(4B5) rabbit monoclonal primary antibody and anti-PR (1E2) rabbit monoclonal primary antibody were purchased from Roche Diagnostics GmbH ...
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bioRxiv - Neuroscience 2024Quote: Primary antibody incubation with rabbit anti-HA antibody was followed by incubation with OmniMap anti-rabbit HRP (05269679001, Roche Diagnostics). Fluorescence signal was detected with FITC fluorophore (07259212001 ...
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bioRxiv - Biophysics 2021Quote: ... Pellets were suspended in 50 ml of denaturation buffer (8 M GdmCl, 50 mM Tris-HCl, pH 8.0) with one protease inhibitor tablet (Roche) per L medium ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma], 0.2 % Nonidet P-40 substitute [Sigma], 1x Complete EDTA-free Protease inhibitor [Roche]) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... beads containing bound methylated DNA were resuspended in 250 μl digestion buffer (50 mM Tris pH 8, 10 mM EDTA, 0.5% SDS) with 100 μg Proteinase K (Roche) and incubated at 65°C for 4 h ...
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bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in TBSI (50 mM Tris, 100 mM NaCl, pH 8, plus Complete Protease Inhibitor Cocktail, Roche) and broken using 0.1 mm silica spheres (Lysing Matrix B ...
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bioRxiv - Molecular Biology 2021Quote: ... washed in cold PBS and incubated on ice in cold swelling buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... washed and homogenized in lysis buffer (20 mM Tris, pH 8, 0.5 mM EDTA and cOmplete protease inhibitor cocktail tablets, EDTA-free (Roche)) using a BeadBeater (Biozym ...
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bioRxiv - Molecular Biology 2021Quote: ... washed with cold PBS and lysed for 20 minutes at 4°C in lysis buffer (10mM Tris pH 8, 10mM NaCl, 0.2% NP-40, supplemented with cOmplete proteinase inhibitors (Roche)) prior to snap freezing in 1ml lysis buffer on dry ice ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then vigorously resuspended in 1ml of fresh ice-cold lysis buffer (50mM Tris pH 8, 150mM NaCl, 0.5% NP-40, 1 tablet of complete protease inhibitor (04693159001, Roche)) and transferred to 9 ml of pre-chilled lysis buffer and incubated for 20 min on ice ...
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bioRxiv - Plant Biology 2019Quote: ... 1.2 mM EDTA, 16.7 mM Tris-Cl [pH 8], 167 mM NaCl, 1x Complete EDTA-free protease inhibitor cocktail [Roche]) and immunoprecipitated a second time using FLAG M2 antibody (Sigma-Aldrich ...
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Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... The fixed embryos were resuspended in 2.5 ml of ice-cold lysis buffer (10 mM Tris-pH-8, 10 mM NaCl, 0.2% NP40 with Roche protease inhibitor cocktail freshly added ...
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bioRxiv - Immunology 2021Quote: ... Pellets of cross-linked cells were resuspended in shearing buffer (10 mM Tris-HCl pH 8, 0.1% SDS, 1 mM EDTA) supplemented with Complete Protease Inhibitor Cocktail (Roche), and chromatin was sheared to an average fragment size of 200-250 bp ...
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bioRxiv - Neuroscience 2020Quote: ... Each tissue sample was dissociated using 2.4 mL of homogenization buffer (10 nM Tris pH 8, 5 mM MgCl2, 25 mM KCl, 250 mM sucrose, 1 μM DTT, 0.5x protease inhibitor [cOmplete, Roche #4693159001] ...
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bioRxiv - Molecular Biology 2020Quote: ... and subjected to 8 cycles of lysis in a MagNALyser (Roche – 6000 rpm, 1 min on, 2 min off). Lysate was transferred to fresh microfuge tubes and clarified by centrifugation (13,000 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... and resuspended in proteome lysis buffer (50 mM Tris HCl pH 8.0, 8 M urea, 75 mM NaCl, 100 mM sodium butyrate, cOmplete protease inhibitors – Roche). Cells were then mechanically lysed (MagNAlyzer Roche – 6000 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... Elution buffer (50 mM Na2HPO4, 0.3 M NaCl, 250 mM imidazole; pH 8) containing EDTA-free protease inhibitors (cOmplete, Roche) was used to elute the proteins ...
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bioRxiv - Biochemistry 2020Quote: ... re-suspended with buffer A (50mM Phosphate buffer pH 8, 400mM NaCl, 5% glycerol, 1mM DTT, 0.5mM EDTA, protease inhibitor cocktail from Roche) and lysed using a microfluidizer followed by two cycles of centrifugation (12000 × g 20 min) ...
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bioRxiv - Microbiology 2019Quote: ... cells were resuspended in 500 μL of urea lysis buffer (8 M urea in 50 mM Tris pH 8.2, 75 mM NaCl, Roche Complete EDTA-free Protease Inhibitor Cocktail tablet ...