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Citations for Roche :
201 - 250 of 557 citations for Cytokeratin 8 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... pH 8) containing a cocktail of protease and phosphatase inhibitors (Roche Applied Science, Mannheim, Germany) was used to extract total proteins from tissue samples and CRC cell lines by its incubation with cell pellets for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 20 mM Tris pH 8) supplemented with 1 mg/mL of protease inhibitors (Roche). Cell lysates were sonicated for 10 cycles with Bioruptor Pico (Diagenode ...
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bioRxiv - Evolutionary Biology 2021Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 594 fluorophores (dilution 1:200 ...
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bioRxiv - Cell Biology 2019Quote: ... 500mM NaCl, 1mM TCEP-HCl (ThermoScientific, 20491) pH 7.4 which was supplemented with recombinant DNase I (1000U) (Roche,04536282001), cOmplete protease EDTA-free inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... 12-20 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) with 2.3 U of Liberase Blendzyme 3 recombinant collagenase (Roche) were cannulated into the vena cava to isolate hepatocytes ...
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bioRxiv - Cell Biology 2022Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 488 fluorophores (dilution 1:200 ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in a 50 mM sodium phosphate buffer at pH 7.8 (buffer A) supplemented with DNase I (100 U, DNase I recombinant, RNase-free Roche), and disrupted using a French Pressure Cell (3 cycles at 1100 psi) ...
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bioRxiv - Immunology 2019Quote: Purified B cells were cultured either in medium alone or in the presence of 1.56-25ng ml− of recombinant BAFF for the indicated time and stained with Annexin V (Roche) and 7-aminoactinomycin D (7-AAD ...
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bioRxiv - Neuroscience 2021Quote: The tissue was dissociated in 300 µL of 0.25% trypsin-EDTA solution supplemented with 10 U/mL recombinant DNase I (Roche) for 15 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and maintained in RPMI GlutaMax supplemented with 10% FCS and 30 U/ml recombinant IL-2 (Roche).
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bioRxiv - Neuroscience 2023Quote: ... and NexCreERT2::R26R-tdT male mice received intraperitoneal injections (i.p.) of recombinant human (rh)EPO (5000 IU/kg body weight; NeoRecormon, Roche) or PL (solvent solution ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
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bioRxiv - Systems Biology 2019Quote: ... 25ml of pre-warmed to 37°C EGTA buffer followed by 25ml of pre-warmed to 37°C EBS buffer with 2.3U of Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) were cannulated into the vena cava ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant protein was expressed in HEK293T cells and released by 100 μM digitonin in PBS with protease inhibitor cocktail (Roche). The cell lysate prepared with the same method from HEK293T was used as the negative control.
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bioRxiv - Microbiology 2021Quote: ... A second DNase I treatment to remove all residual genomic DNA was performed using 30 U of recombinant DNase I (Roche) and 10 U of Protector RNase inhibitor (Roche ...
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bioRxiv - Immunology 2020Quote: ... The treated samples were purified using a C18 cartridge (Oasis HLB Plus Waters) prior to the release of N-glycans by PNGase F (recombinant from Escherichia coli, Roche) digestion ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 mL of Sf9 cells at a density of 1.2×106 cells/mL were transfected with 5 µL of recombinant bacmid containing the gene of interest using 3 µL of X-tremeGENE HP DNA transfection reagent (Roche) and 100 µL of transfection medium (Expression Systems) ...
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bioRxiv - Neuroscience 2021Quote: ... MGEs pooled from a single litter were incubated with 200 µL papain solution supplemented with 10 U/mL recombinant DNase I (Roche) for 10 minutes at 37 °C on a rocking platform ...
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bioRxiv - Molecular Biology 2021Quote: ... An equal volume of lysis buffer and 110 U/ml of DNase (DNase I recombinant, RNase-free solution, Cat no. 4716728001, Roche) was added to the ground cell lysate followed by incubation for 1 hour at 4°C ...
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bioRxiv - Genetics 2022Quote: ... MYC-tagged or FLAG-tagged recombinant proteins were captured using ~5μg antibody and Protein-Agarose beads (Roche Applied Science, 11243233001) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Positive controls were generated by incubating some sections from control 3 dpf animals with recombinant DNAse I (400 U/ml; Roche) for 20 minutes at room temperature before the incubation in the reaction mix ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR enriched (8-10 cycles) using the KAPA Hyper Library Preparation kit (KAPA Biosystems, #KK8504). eCLIP libraries were prepared as described above according to (Van Nostrand et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... 50 mM Tris-HCl pH 8) supplemented with protease and phosphatase inhibitor cocktail (Roche Molecular Biochemicals), and at 4 °C with gentle shaking/tumbling for 30 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 mM Tris-HCl pH 8 supplemented with phosphatase and protease inhibitor cocktail tablets (Roche Diagnostics)) ...
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bioRxiv - Neuroscience 2021Quote: ... 8 μL of each sample E3 was used directly for PCR as described above (KAPA Biosystems). Embryos were kept in 48-well plates until genotyping was achieved.
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bioRxiv - Cell Biology 2019Quote: ... the slides were incubated for 5 h at 8°C with mouse anti-DIG antibody (Roche, Basel ...
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bioRxiv - Microbiology 2020Quote: ... pH 8) supplemented with protease inhibitors (1 tablet of complete protease inhibitor per 20 mL; Roche, Mannheim ...
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bioRxiv - Cell Biology 2021Quote: ... overnight cultures were diluted to OD600 = 0.1 and treated with 8 mM DTT (Roche, Mannheim, Germany) for up to 2 h ...
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bioRxiv - Molecular Biology 2019Quote: ... gDNA was amplified using SV40_NLS_seq_f and SV40_NLS_seq_r (Supplementary Table 8) primers using Kapa Hifi (Kapa Biosystems KK2602) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The pellet was resuspended in 8 mL of polysome buffer containing 1x Protease inhibitor Cocktail (Roche) and 1 mM PMSF ...
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bioRxiv - Microbiology 2019Quote: ... 80mM EDTA pH 8 and complete EDTA-free protease inhibitor cocktail according to instructions (Roche, CH)) ...
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bioRxiv - Immunology 2021Quote: ... 50 mM Tris-Cl (pH 8) supplemented with Complete Protease Inhibitor Cocktail tablet (Roche, Mannheim, Germany) and 1x Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 μL of extraction solution (8 M Urea, 25 mM NH4HCO3, Protease inhibitor (cOmplete tab, Roche Switzerland ...
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bioRxiv - Developmental Biology 2023Quote: ... Each sample was lyzed in 200 mM HEPES buffer (pH 8) containing PhosSTOP phosphatase inhibitors (Roche), phosphatase inhibitor cocktail2 (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... pH 8) containing 10 mM Imidazole and supplemented with Protease Inhibitor Mixture (Complete, Roche Applied Science) for lysis at 4 °C by sonication ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Triton-X 100 at pH 8) supplemented with EDTA-free complete protease inhibitors (Roche, 56619600). Lysozyme was added to 1 mg/mL and lysate incubated for 20 min on ice ...
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bioRxiv - Genetics 2024Quote: ... Total RNA was extracted from 5-8 pairs of ovaries using an RNA extraction kit (Roche). 50 ng of total RNA was used to make cDNA using the Transcriptor First Strand cDNA Synthesis kit (Roche ...
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bioRxiv - Genetics 2021Quote: ... rabbit anti-Myc (Roche, 1:1,000); rat anti-HA (Roche ...
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bioRxiv - Microbiology 2020Quote: ... DNA was first eluted from the column in 100 µL DNA elution buffer and subsequently the column was treated with recombinant DNase I (20 units/100 µL; Roche Diagnostics) for 30 min at 37°C and finally RNA was eluted in 50 µL nuclease free water ...
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bioRxiv - Immunology 2021Quote: ... PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM) supplemented with human recombinant EPO (2 U/ml, Roche), human recombinant stem cell factor (25 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Two milligrams recombinant MYC and 12 μl T7-HCF-1VIC were rotated overnight at 4°C in Kischkel buffer + PIC (Roche 05056489001). Anti-FLAG M2 Affinity Gel (Sigma-Aldrich) ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... 1 mM EDTA pH 8 and 1 tablet of protease inhibitor cocktail Roche cOmplete (Roche, Basel, Switzerland) and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... Tissues were lysed with 8 M urea in PBS supplemented with protease and phosphatase inhibitor cocktail (Roche) at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was probed with a 1:20000 dilution of mouse anti-GFP antibody (JL-8, Roche) or mouse anti-alpha-tubulin antibody (B-511 ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 mM Tris pH 8) containing protease inhibitors (Roche mini EDTA-free, 1 tablet in 10 ml) and phosphatase inhibitors (2mM Sodium Ortho-Vanadate ...
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bioRxiv - Developmental Biology 2021Quote: ... we lysed samples in 8 μl of lysis buffer PBND containing 0.1 mg/ml Proteinase K (Roche) (Scavizzi et al. ...
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bioRxiv - Microbiology 2022Quote: ... and lentivirus vectors at a 2:8:10 μg ratio to HEK293T cells using Xtreme-Gene9 (Roche) and lentivirus-containing supernatants were collected 72hr post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 ml ice-cold 8 % (v/v) paraformaldehyde solution in vPBS containing EDTA-free protease inhibitor (Roche) or ice-cold 8% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 mM EDTA (pH 8)) supplemented with 2 mM Na3VO4 and complete protease inhibitor mixture (Roche). Lysates were first centrifuged at 4 °C for 15 min at 20.000 g and subsequently subjected to ultracentrifugation at 4° C for 30 min at 100.000 g ...