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Citations for Roche :
351 - 400 of 789 citations for Adenovirus Type 40 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) and sonicated for 90 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Adipocytes were washed and lysed in a buffer (10mM Tris-HCl pH8.0, 10mM NaCl, 0.2% NP-40, 1x cOmplete Protease Inhibitor Cocktail from Roche) on ice for 30 min with shaking ...
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bioRxiv - Microbiology 2019Quote: ... Crosslinked cell pellets were mixed with 1 mL fractionation buffer (5mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40 with cOmplete EDTA-free Protease Inhibitors [Roche]) and incubated on ice for 10 min ...
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bioRxiv - Microbiology 2019Quote: Cells were lysed in RIPA buffer (50 mM Tris-HCl pH7.6, 150 mM NaCl, 3 mM MgCl2, 10% glycerol, 0.5% NP-40, cOmplete EDTA-free Protease Inhibitors [Roche]) and then clarified by centrifugation at 21,000 × g for 10 min at 4°C ...
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bioRxiv - Immunology 2019Quote: ... pH 7.4, 150 mM NaCl, 0.25% deoxycholic acid, 1% NP-40 and 0.5% SDS supplemented with protease inhibitor [Roche]) and centrifuged at 12,000 g at 4°C for 10 min ...
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bioRxiv - Genetics 2019Quote: Cells were lysed in EBC buffer (50 mMTris pH 7.5, 120 mMNaCl, 0.5% NP-40) supplemented with protease inhibitors (Complete Mini, Roche) and phosphatase inhibitors (phosphatase inhibitor cocktail set I and II ...
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bioRxiv - Neuroscience 2021Quote: ... 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA and 1X protease inhibitor cocktail; Roche). Anti-FLAG M2 Magnetic Beads (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: Transfected HeLa were harvested and lysed with lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5% NP-40, protease inhibitors tablet from Roche, 50 mM NaF ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl, 1 mM EDTA, 5mM MgCl2, 0.5% NP-40, pH 7.4, supplemented with Complete Protease Inhibitor Cocktail, Roche). After centrifugation at 5000g for 10minutes ...
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bioRxiv - Microbiology 2021Quote: ... cell extracts were lysed in TNN lysis buffer (100mM Tri-HCl pH8.0, 250 mM NaCl, 0.5% NP-40, and cOmplete protease inhibitor (Roche)) and centrifuged for 7 min at 15’000 rpm and 4°C ...
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bioRxiv - Molecular Biology 2020Quote: Adherent cells were washed twice with PBS and then scraped and digested on ice for 30 min with a homemade RIPA (50 mM Tris-HCl pH 7.5, 15 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS, supplemented with Protease Inhibitors [Roche] and Phosphatase Inhibitors [Sigma-Aldrich]) ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with RIPA buffer (25 mM Tris HCl [pH 7.6], 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche]). The lysates were subjected to SDS-PAGE under denaturing conditions in 12% polyacrylamide gels using Bio-Rad Mini PROTEAN electrophoresis system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: Cells transfected with indicated constructs were harvested and lysed in NETN buffer (10mM Tris-HCl [pH 8.0], 100mM NaCl, 1mM EDTA, and 0.5% NP-40) plus protease inhibitor (Roche) on ice for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... and 0.5% (w/v) polyvinylpyrrolidone-40 supplemented with a protease inhibitor cocktail (cOmplete, EDTA-free; Roche, Basel, Switzerland). After the removal of cellular debris ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with 40 µL of primary antibody (1:1,000 rat anti-HA mAb 3F10, Roche) at 4°C overnight in a solution containing 3% BSA in PBS ...
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bioRxiv - Genetics 2022Quote: ... 0.05% NP-40) + protease inhibitors (Complete Ultra Tablets, Mini Protease Inhibitor Cocktail; Roche #05892791001 and 1mM PMSF; Sigma). Worms were allowed to settle at the bottom of the tube and the lysis buffer was removed until there was 0.5-1ml buffer covering the worm pellet ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were lysed with NETN-300 buffer (20mM Tris-HCl Ph8.0, 300mM NaCl, 1mM EDTA, 0.5% Nonidet P-40) containing protease inhibitor cocktail (Roche), 3μM TSA and 50mM NAM on ice for 10min ...
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bioRxiv - Cell Biology 2021Quote: ... 50 mM Tris, 0.1% v/v SDS, 0.5% v/v NP-40 pH8, 1x complete protease inhibitor, Roche) with stainless metal beads (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... the cells were thawed on ice followed by lysis using Farham lysis buffer (5 mM PIPES pH 8.0, 85mM KCl, 0.5% NP-40 supplemented with protease and phosphatase inhibitors (Roche)) made in miliQ ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was aspirated and the pellet was resuspended in RIPA buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS supplemented with Roche protease and phosphatase inhibitor tablet just before use) ...
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bioRxiv - Genomics 2020Quote: ... Cells were collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 0.1% SDS, 2 mM EDTA, 1 mM PMSF, Roche protease and phosphate inhibitor cocktails ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM Tris, pH 7.5, 1 mM EDTA, 0.5% Nonidet P-40, protease and phosphatase inhibitor cocktail [Roche]). Cell lysates were denatured in Laemmli sample buffer and incubated for 5 min at 95°C ...
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bioRxiv - Physiology 2020Quote: ... 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, protease inhibitors [Roche], phosphatase inhibitors [Roche]). Lysates were incubated for 15 minutes on ice and centrifuged twice at 15,000 x g for 20 minutes at 4°C to remove insoluble material ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS)39 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was removed and the pellet was dissolved in 500 µl of ice-cold nuclear solubilization buffer (25 mM HEPES [pH 6.8], 1 M sorbitol, 0.5% NP-40, 250 μg/ml PMSF, cOmplete [EDTA-free protease inhibitor cocktail; Roche]). Following incubation for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... cell extracts were lysed in TNN lysis buffer (100mM Tri-HCl pH8.0, 250 mM NaCl, 0.5% NP-40, and cOmplete protease inhibitor (Roche)) and centrifuged for 7 min at 15’000 rpm and 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... For enteric glial cell experiments were performed by hypotonic lysis in 200 µl of buffer A (20 mM HEPES, pH 8.0, 1 mM DTT, 0.1% NP-40 and a proteinase inhibitor cocktail from Roche). The cell lysates were incubated on ice for 10 minutes ...
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bioRxiv - Molecular Biology 2019Quote: Crosslinked nuclei were extracted in 0.8ml modified RIPA lysis buffer (50mM Tris, 150mM NaCl, 0.5% sodium deoxycholate, 0.2% SDS, 1% NP-40, supplemented with: 1x antiprotease (Roche), RNasin (Promega) ...
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bioRxiv - Microbiology 2019Quote: ... The total protein pellets were resuspended in 1 mL NP-40 with protease inhibitor cocktail (Roche Diagnostics GmbH). Samples were pre-cleared using Protein A/G plus agarose beads (Pierce ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were lysed with Farnham Lysis Buffer (5mM PIPES at pH 8.0, 85 mM KCl, 0.5% NP-40) containing protease inhibitor (Roche). Cell lysate was centrifuged at 2,000 rpm for 5 min at 4 °C ...
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bioRxiv - Microbiology 2019Quote: Cells were lysed in 1× lysis buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 0.5% NP-40, cOmplete EDTA-free Protease Inhibitors [Roche]) by rotating for 30 min at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... 1 mM EDTA and 0.5% NP-40 supplemented with Complete Mini EDTA-free Protease inhibitor cocktail tablets (Roche)) and left to incubate for 20 minutes on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 mM Tris-HCl pH 7.4, 5 mM EDTA, 5 mM MgCl2, 1% NP-40, 0.5 mM DTT, Roche mini-tablet protease inhibitor ...
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bioRxiv - Developmental Biology 2021Quote: ... washing the pellet in PBS and resuspending the cells in ice-cold 10 ml buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.1% NP-40, c0mplete Mini Protease inhibitor EDTA free from Roche) and rotating for 10 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Cells were harvested and lysed in NP-40 buffer (50 mm TRIS, pH 8.0, 150 mm NaCl, 1.0% NP-40 with Complete Protease Inhibitor Cocktail from Roche). For Western blots ...
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bioRxiv - Microbiology 2021Quote: ... and polymerized by UV light prior to labeling with anti-HA antibody (Roche, clone 3F10, 1:40 dilution) and 10nm gold bead conjugated goat anti-rat (Electron Microscopy Sciences ...
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bioRxiv - Genetics 2020Quote: ... and suspended in 1 mL of ice-cold protein extraction buffer (50mM HEPES pH 7.5, 150mM NaCl, 0.02% NP-40, 1mM EDTA, 1mM phenylmethylsulfonyl fluoride [PMSF; Sigma, P7626], one tablet Roche cOmplete mini EDTA-free Protease Inhibitor Cocktail [Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells are pelleted and resuspended in 1 mL of Lysis Buffer (200 mM NaCl, 25 mM HEPES, 1 mM MgCl2, 0.2 mM EDTA, 0.5% NP-40, 1X Roche complete inhibitor ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed with PBS twice and lysed with lysis Buffer II (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.5% NP-40, 1 mM EDTA and EDTA-free protease inhibitors cocktail [Roche]). As a control of protein synthesis inhibition ...
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bioRxiv - Molecular Biology 2020Quote: ... Fixed cells were resuspended in 10 ml ice cold buffer LB1 (50 mM Hepes-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.50% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Genetics 2019Quote: ... Samples were then incubated at 55 °C for 1h after addition of 40 μg of Proteinase K (Roche) and ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM MgCl2, 0.5% Triton X-100, 0.25% Nonidet P-40 and 10% glycerol, supplemented with protease inhibitors [Roche]). After a 10 minute incubation on ice ...
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bioRxiv - Microbiology 2020Quote: Intracellular expression of APOBEC3 proteins during virion production was determined by lysis of the virion-producing 293T cells with NP-40 buffer with protease inhibitor (200mM NaCl, 50mM Tris pH 7.4, 0.5% NP-40 Alternative, 1mM DTT, and Roche Complete Mini ...
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bioRxiv - Microbiology 2020Quote: Whole cell lysates were generated by lysing cells in RIPA buffer (50 mM Tris-Cl [pH 7.4], 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 1 mM phenylmethylsulfonyl fluoride [PMSF], 1× Roche complete mini-protease inhibitor cocktail ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet was suspended in 1 mL of cold lysis buffer (50 mM TRIS-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, 1 tablet of Roche cOmplete Protease inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% NP-40 in 1X PBS) plus protease inhibitor (cOmplete, Mini Protease Inhibitor Cocktail Tablets, EDTA free, Roche) on ice for 10 minutes and clarified by centrifugation at 15,000 rpm for 20 minutes at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: Protein lysates were prepared from snap-frozen dorsal skin with urea buffer (8 M Urea, 40 mM Tris-HCl, 2.5 mM EDTA, pH 8.0) containing phosphatase (PhosSTOP, Roche) and protease inhibitors (cOmplete Protease Inhibitor Cocktail Tablet EDTA-free ...
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bioRxiv - Microbiology 2020Quote: ... and lysed by resuspension in lysis buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.4, 1 mM EDTA, 0.5% NP-40, and protease inhibitor [Roche]) with rotation at 4°C for 30 min ...