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Citations for Roche :
601 - 650 of 789 citations for Adenovirus Type 40 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Protein was harvested from cell pellets lyzed using 2x pellet volume NP-40 lysis buffer (0.5% Nonidet P-40, 50mM Tris [pH 7.8], and 150mM NaCl) supplemented with protease inhibitor (Roche Molecular Biochemicals) and phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Plant Biology 2022Quote: ... 200 mM NaCl, 1 mM EDTA, 1%NP-40, 1 mM DTT, 10 mM MgCl2, 1 × protease inhibitor cocktail from Roche) for 2 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... cells or brain tissue were lysed in mild lysis buffer (50mM Tris-HCl, 150mM NaCl and 1% NP-40) with cOmplete mini protease inhibitor cocktail (Roche) and centrifuged at 12 000g for 10 minutes at 4 °C ...
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bioRxiv - Physiology 2022Quote: Mouse platelets were isolated using modified standard procedures,[50] as previously outlined.[44] Washed platelets were resuspended directly in NP-40 lysis buffer containing Complete protease inhibitors (Roche). Glomeruli were isolated using magnetic bead perfusion as previously described,[48 ...
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bioRxiv - Microbiology 2022Quote: ... and cells were lysed in assay buffer (1% NP-40) supplemented with cOmplete™ mini EDTA-free protease inhibitor (Roche) and PhosSTOP phosphatase inhibitor (Roche) ...
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bioRxiv - Microbiology 2020Quote: Cells were washed once with ice-cold PBS and lysates harvested at the indicated times post infection with lysis buffer (1% NP-40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris HCl) supplemented with protease inhibitors (Roche – complete mini EDTA-free protease inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... Equal amounts (Protein estimated by Bradford) of soluble fractions were incubated with 40 μl of pre-equilibrated anti-HA affinity agarose gel (Roche) in binding buffer (Lysis buffer) ...
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bioRxiv - Molecular Biology 2020Quote: 50 million cell pellets were resuspended in 2.5ml ice cold Hi-C Lysis Buffer (10mM Tris HCl, 10mM NaCl, 0.2% NP-40, 1X protease inhibitors (Roche, 04693124001)) and split into 10 million cell amounts ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1% SDS,1% NP-40 and 1% Triton X-100) supplemented with 1 mM PMSF and protease inhibitor cocktail (Roche) at 4°C for 20 min ...
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bioRxiv - Immunology 2019Quote: ... Lysis buffer was added (40 mM HEPES, 140 mM NaCl, 0.1% Triton-X-100, Roche EDTA-free complete protease inhibitor) and cells were detached with a cell scraper ...
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bioRxiv - Microbiology 2019Quote: ... Protein extracts were incubated for 3 h on a spinning wheel at 4°C with 40 µl of Protein G Sepharose beads (Roche) and 2.5 µg of the specific BTV-NS3 antibody ...
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bioRxiv - Genomics 2019Quote: ... amplicons were diluted 1:100 and 1 µL was used into a 40-µL barcoding reaction using 20 µL 2x KAPA HiFi Hotstart reagents (Roche) and 80pmol each barcoded primer ...
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bioRxiv - Plant Biology 2019Quote: ... 60 mM KOAc, 10 mM MgOAc, 0,5% [v/v] Nonidet P-40 and proteinase inhibitor cocktail [cOmplete™, COEDTAF-RO, Roche]). After a solubilization step (15 min ...
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bioRxiv - Biochemistry 2019Quote: ... were lysed with lysis buffer (25mM Tris, 150mM NaCl, 1mM EDTA, 1% NP-40, 5% Glyceral) with proteinase inhibitor cocktail (Roche) on ice for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 20% glycerol, 0.05% NP-40, 0.2 mM EDTA, 0.2 mM PMSF, 0.5 mM DTT, and Roche complete protease inhibitor tablets). The frozen extract was thawed ...
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bioRxiv - Immunology 2020Quote: ... supplemented with BaseMuncher nuclease (Expedion; 1 ml per 40 ml cell suspension) and Complete EDTA-free protease inhibitor mix (Roche). The extract was precleared by centrifugation ...
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bioRxiv - Systems Biology 2020Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Molecular Biology 2019Quote: 1×106 cells were lysed in 50 μl NP40 lysis buffer (0.5% Nonidet P-40, 50 mM Tris, pH 7.8, 150 mM NaCl) supplemented with protease inhibitor (Roche Molecular Biochemicals) and phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Microbiology 2019Quote: Immunoprecipitation was performed using IP buffer (1% Nonidet P-40, 50mM Tris-HCl [pH 7.5], 150mM NaCl, and Complete™ protease inhibitor cocktail-EDTA (Roche)) ...
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bioRxiv - Genetics 2021Quote: ... 3.2mM MgCl2, 0.25% Triton X-100, 0.25% NP-40, 0.1% Na-deoxycholate and 1mM DTT and Roche Complete protease inhibitor). To this 4ul of Benzonase (E1014 ...
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bioRxiv - Genetics 2021Quote: ... 3.2mM MgCl2, 0.25% Triton X-100, 0.25% NP-40, 0.1% Na-deoxycholate and 1mM DTT and Roche Complete protease inhibitor), 2ul of Benzonase (E1014 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% Nonidet P-40, 1 mM sodium orthovanadate and 1× complete protease inhibitor cocktail from Roche) at 4°C for 15 minutes ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 0.5% Nonidet P 40 Substitute (NP40; Fluka Analytical) and cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche)] ...
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bioRxiv - Biochemistry 2021Quote: The confluent cells were washed with PBS and lysed with ice-cold NP-40 Lysis Buffer supplemented with protease inhibitor cocktail (Roche) and PhosSTOP (Roche) ...
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bioRxiv - Biochemistry 2021Quote: Cells confluent in a 15-cm dish were washed by PBS buffer three times before being lysed on plate with 1 mL ice-cold NP-40 lysis buffer supplemented with protease inhibitor cocktail (Roche) and PhosSTOP (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: Naïve and activated B cells were incubated on ice for 20 min in NP-40 lysis buffer in the presence of protease inhibitors (Roche) and lysates were cleared by centrifugation ...
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bioRxiv - Developmental Biology 2021Quote: ... The testicular tissue was finely minced and incubated in a dissociating solution containing 40 mg/mL Collagenase H (Roche, Switzerland), 33.3 mg/mL Dispase II (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: Protein from cell pellets was extracted with 2x pellet volume protein lysis buffer (0.5% Nonidet P-40, 50mM Tris [pH 7.8], and 150mM NaCl) supplemented with protease inhibitor (Roche Molecular Biochemicals) and phosphatase inhibitor cocktail (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were washed once with PBS and resuspended in ice-cold WCE buffer (10 mM Tris-HCl, 1% NP-40, 2 mM MgCl2, benzonase, cOmplete Protease Inhibitor Cocktail (Roche), 25 nM NEM where relevant ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mM ortho-vanadate) supplemented with 0.5% Surfact-AMPS NP-40 (Thermo, Waltham MA, #85124) and 1x Complete Protease Inhibitor (Roche, #11697498001) for 20 min at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed with SLB lysis buffer (10 mM Tris, 150 mM NaCl, 0.5% NP-40) with protease inhibitors (Roche #11873580001) and phosphatase inhibitors (Roche #04906837001) ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed pellets were resuspended with 200-300 µL native lysate buffer (1% NP-40 with 1X cOmplete protease inhibitor cocktail [SKU, 11836145001, Roche] ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor and tumoroid sections were in-cubated for 40 min with the appropriate antibody before incubation with Discovery UltraMap anti-Rabbit (760–4315, Roche) or anti-mouse horseradish peroxidase (HRP ...
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bioRxiv - Bioengineering 2022Quote: Ileal and colonic proteins were obtained by tissue homogenization in NP-40 Buffer with 1x cOmplete Mini Protease Inhibitor Cocktail (Roche). Protein concentrations were determined using Bradford Assay ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were diluted 1:4 with ice-cold dilution buffer (1× phos-stop [Roche], 0.5% NP-40, 1 mM DTT) and centrifuged again at 15,000 rpm for 15 minutes at 4°C to precipitate actomyosin components ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspension was generated by mechanically disrupting tissues through a 100µm filter into FBS followed by a Percoll gradient centrifugation (40% Percoll, GE Healthcare; FBS free media containing 0.5mg/ml DNase, Roche). Cells were then washed with complete media and analyzed by flow cytometry.
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bioRxiv - Genomics 2022Quote: ... The cell pellet was lysed in cell lysis buffer (20 mM Tris-HCl pH 8, 85 mM KCl, 0.5% NP-40) supplemented with 1X protease inhibitors (Roche, 11836170001) on ice for 20 minutes then spun at 5000 rpm for 10 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... total proteins were extracted with lysis buffer (10 mM Tris-Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 100 mM PMSF, and 1X Roche protease inhibitor cocktail), followed by incubated with anti-GFP beads (AB_2631358 ...
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bioRxiv - Plant Biology 2022Quote: ... 10% [v/v] glycerol, 10 mM EDTA, 1% [v/v] NP-40, and complete EDTA-free protease inhibitor cocktail [Roche]). The extracts were centrifuged at 20,000 × g at 4°C for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was dounce homogenized in a nuclear extraction buffer (10mM Tris, 146mM NaCl, 1mM CaCl2, 21mM MgCl2, 0.1% NP-40, 40u/mL Protector RNAse inhibitor (Roche 3335399001). Dounce homogenizer was washed with 4mL of a washing buffer (10mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM EDTA, 0.1% NP-40, 1 mM DTT, 10 mM NaF, and EDTA-free protease inhibitor cocktail, Roche, Germany), and centrifugation at 20,000 × g for 3 min ...
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bioRxiv - Genomics 2023Quote: Chromatin samples prepared from 40 million ES or 501 Mel cells were supplemented with protease inhibitors (Complete Protease Inhibitor Cocktail, Roche) and loaded on a 9.9 ml manually poured 10-30 % sucrose gradient containing 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: Cells and tissues were lysed in NP-40 lysis buffer or SDS lysis buffer supplemented with protease inhibitor (Roche, 04693159001) and then centrifuged at 4 °C to obtain cellular lysate ...
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bioRxiv - Cell Biology 2022Quote: HK cells were lysed using a lysis buffer (50mM Hepes, 130mM NaCl, 1mM DTT 1% NP-40) with complete protease inhibitor cocktail (Roche). Cell lysates were centrifuged at 13,000 rpm for 10 mins at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining extract was centrifuged at 2,000 rpm for 5 minutes and re-suspended in 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete), sonicated and stored at -20°C for downstream applications.
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysis buffer (150 mM NaCl, 1 mM EDTA, 50 mM Tris-HCl, 1% NP-40 with proteases and phosphatases inhibitors (Roche)) was added directly to the wells and cells were scraped and collected in pre-cooled Eppendorf tubes followed by sonification for 10 min ...
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bioRxiv - Cell Biology 2023Quote: Decapsulated testis extract was re-suspended into 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete) and sonicated for 20 s at 22% amplitude in cycles of 0.4 s on and 0.2 s off ...
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bioRxiv - Cancer Biology 2023Quote: ... Thawed pellets were lysed in LB1 (50 mM HEPES-KOH pH 7.6, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.5% NP- 40, 0.25% Triton X-100, Roche protease inhibitor cocktail) for 20 min with rotation at 4°C and cleared by centrifugation at 300 x g for 5 min at 4°C ...