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Citations for Roche :
651 - 700 of 3928 citations for 8 4 Chlorophenylthio 2' O methyladenosine 3' 5' cyclic monophosphate sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 300 mM NaCl and 2 mM β -mercaptoethanol) per 5 × 108 cells in the presence of EDTA-free anti-protease cocktail (complete from Roche). Lysis was performed with two cycles of freezing (− 180 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription (RT) was performed from 2-3 µg of RNA with the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Sciences, Basel, Switzerland) using random hexamer primers ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The Block C and Block O blocking oligos were replaced with an equivalent volume of SeqCap EZ Developer Reagent (Roche Diagnostics, Mannheim, Germany). Each reaction was amplified for 16 cycles ...
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bioRxiv - Cancer Biology 2020Quote: ... All the signals were visualized by adding 3-3′-Diaaminobenzidinetetrahydrochloride (DAB substrate) solution (Roche) to the slides and counterstained with haematoxylin ...
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bioRxiv - Biophysics 2021Quote: ... Sf9 cell pellets were resuspended in low salt buffer (50 mM HEPES, pH 7.5, 50 mM NaCl, 0.5 mM EDTA and protease inhibitor cocktails (Roche) and lysed by Dounce homogenization (∼30 strokes) ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting pure nuclear pellet was first resuspended with 70 μL low salt buffer (20 mM HEPES, pH 7.6, 1.5 mM MgCl2, 10 mM KCl, 25% glycerol, protease inhibitor (Roche)) and 2.5 μL benzonase (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... High Salt buffer (50 mM HEPES pH 7.5, 500 mM NaCl, 1% Triton X-100, 1x protease (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissues were washed and the secondary antibodies applied (dilutions listed below) with 4′-6-diamidino-2-phenylindole (DAPI: Cat # 10-236-276-001, Roche Diagnostics, Indianapolis, IN) at 1:1,000 for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in SDS-PAGE loading buffer (50 mM TrisC1 pH 6.8, 2% SDS, 0.1% bromophenol blue, 10% glycerol, 4% β-mercaptoethanol, 1 mM PMSF, 1x Roche cOmplete protease inhibitor cocktail) and boiled for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... Nematodes were resuspended in 8 M urea in 100 mM TRIS-HCl buffer (pH 8) containing cOmplete™ protease inhibitor cocktails (Roche Diagnostic Canada, Laval, QC, Canada) and aliquoted into reinforced 1.5 mL homogenizer tubes containing 500 μm glass bead homogenizer tubes with 25 mg of glass beads ...
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Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesisbioRxiv - Evolutionary Biology 2022Quote: ... and 3% Blocking Reagent (Roche) to the hybridization mix during overnight blocking and hybridization of the probe ...
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bioRxiv - Developmental Biology 2023Quote: ... and 3% Blocking Reagent (Roche) to the hybridization mix during overnight blocking and hybridization of the probe ...
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bioRxiv - Cancer Biology 2021Quote: ... were prepared using the IP buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% TritonX-100, 0.5% Na-DOC, 1 mM EDTA, 2 mM PMSF and 1x Roche protease inhibitor cocktail). After sonification for 5 min and clarification with centrifuge at 16,000 g at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... on a gentleMACS Octo Dissociator (Miltenyi) using the “Protein_01_01” protocol in MACS buffer (5 mM CaCl2, 2 mM EDTA, 1X protease inhibitor (Roche, 05-892-970-001), 300 mM MgAc ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellets were lysed in 200μL of lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 5 mM MgAc, 2% digitonin, and 1X Roche protease inhibitor cocktail) by incubating on ice for 30 min and then diluted to 1ml with the lysis buffer containing 0.1% digitonin ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in buffer F (20 mM Tris pH 7.5, 100 mM NaCl, 5 mM MgCl2, 2 mM EGTA and Roche cOmplete protease inhibitor) and lysed by fluidizer ...
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bioRxiv - Cell Biology 2022Quote: ... and then homogenized on a gentleMACS Octo Dissociator (Miltenyi) using the “Protein_01_01” protocol with MACS buffer (5 mM CaCl2, 2 mM EDTA, 1X protease inhibitor (Roche, 05-892-970-001), 3 mM MgAc ...
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bioRxiv - Biophysics 2021Quote: ... Cell pellets were resuspended in Lysis Buffer (25 mM HEPES pH 8.0, 250 mM NaCl, 10 mM imidazole pH 8.0, 5 mM 2-mercaptoethanol, 10% glycerol and supplemented with Roche cOmplete protease inhibitor) and sonicated ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was resuspended in Buffer B (10 mM Pipes, 50 mM KCl, 5 mM MgCl2, 1 mM DTT, 2 mM PMSF, Roche protease inhibitor) with 1% Triton X-100 and incubated on ice for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... autofluorescence was quenched by treating paraffin-embedded sections with PBS/BSA (5%) for 2 h before performing TUNEL staining (Roche, Basel, Switzerland) according to the manufacturer’s protocol and using Proteinase K treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... 20mM Tris-HCl pH 8) supplemented with protease inhibitors (Roche, 04693116001), followed by a final washing step with TE 1X buffer ...
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bioRxiv - Biochemistry 2019Quote: ... pH 8) containing complete protease inhibitor (in 50ml) cocktail (Roche Molecular) and disintegrated with a French press (1100 psi) ...
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bioRxiv - Cell Biology 2020Quote: ... the specimen was digested in 8 mg/mL Collagenase D (Roche) and 4.8 U/mL Dispase II (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... pH 8] with EDTA-free Protease Inhibitor Cocktail (Roche, Basel, Switzerland) and were clarified by centrifugation ...
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bioRxiv - Molecular Biology 2019Quote: ... all at pH 8) supplemented with a phosphatase (Roche, Basel, Switzerland) and protease inhibitor cocktail (Roche) ...
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bioRxiv - Neuroscience 2019Quote: ... 8 U/ml RNAse inhibitor and a protease inhibitor cocktail (Roche). Cell debris and nuclei were removed by centrifugation at 1000 g for 10 min at 4°C yielding pellet P1 and supernatant S1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... pH 8 with added inhibitors (cOMPLETE™ tablets, EDTA-free, Roche), DNAse (1 mg/ml) ...
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bioRxiv - Cancer Biology 2019Quote: ... with filters (8-μm pore size) pre-coated with fibronectin (Roche). APOC2 overexpressing cells ...
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bioRxiv - Neuroscience 2021Quote: ... 20 mM Tris HCl pH 8 with protease inhibitor cocktail (Roche) and assayed for protein concentration by the BCA assay (Pierce) ...
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bioRxiv - Biophysics 2023Quote: ... pH 8) with cOmplete EDTA-free protease inhibitor cocktail (11873580001, Roche), 70 µg/mL lysozyme (90082 ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% sodium dodecyl sulphate (SDS) with protease inhibitors (Complete Mini, EDTA-free, Roche)] ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.25% sodium-deoxycholate and 50 mM Tris and complete protease inhibitors (PIM; Roche). Lysates were sonicated (Bioruptor pico ...
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bioRxiv - Neuroscience 2020Quote: ... 0.5% sodium deoxycholate and 0.1% SDS) plus a complete protease inhibitor cocktail (Roche). Lysates were centrifuged and supernatants were subjected to SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... 10 mM sodium pyrophosphate and protease inhibitor cocktail (complete mini, EDTA-free, Roche). Cells were lysed under denaturing conditions by bead-beating with a chilled MiniBeadbeater (Biospec ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.1% sodium deoxycholate) supplemented with 1mM PMSF and 1x protease inhibitor cocktail (Roche). Samples were aliquoted in Eppendorf tubes and frozen ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... and 0.5% sodium deoxycholate) supplemented with EDTA-free protease inhibitor (Roche Applied Science) and PhosSTOP phosphatase inhibitor (Roche Applied Science ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.1% sodium deoxycholate) supplemented with 1X complete mini EDTA protease inhibitor cocktail (Roche), incubated on ice for 15 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 mM sodium pyrophosphate in PBS) mixed with protease and phosphatase inhibitors (Roche). Equal amounts of protein were boiled ...
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bioRxiv - Cell Biology 2020Quote: ... 0.25% Sodium Deoxycholate) supplemented with Complete protease inhibitor and PHOSStop phosphatase inhibitor (Roche). Hundred micrograms of protein were separated in 10% SDS PAGE and transferred to a PVDF membrane ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% Sodium dodecyl-sulphate in ultrapure water) supplemented with Protease inhibitor (Roche: 11873580001) and Phospho-STOP (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM sodium vanadate and a protease inhibitor cocktail (Roche Pharmaceuticals, Nutley, NJ). Proteins were separated by SDS-PAGE with 4-20% gradient gels (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... 1% sodium deoxycholate and 0.1% SDS) supplemented with protease inhibitor cocktail (Roche #11697498001) and PMSF (Beyotime #ST507 ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1% sodium dodecyl sulphate) supplemented with cOmplete™ ULTRA Protease Inhibitor (Roche; #5892953001) and PhosSTOP™ Phosphatase Inhibitor (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1 % sodium doceylsulphate (SDS) pH = 7.5) supplemented with a protease inhibitor cocktail (Roche) and 200 mM phenylmethylsulphonyl fluoride (PMSF) ...
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bioRxiv - Systems Biology 2023Quote: ... pH 7.4) supplemented with 10 mM sodium ascorbate and protease inhibitors (Roche Complete). Samples were briefly sonicated ...
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bioRxiv - Microbiology 2019Quote: ... Cells were lysed in 100 µl lysis buffer (5 ml contain: 10 mM Tris-HCl pH 7.5, 4% SDS, 1 PhosSTOP tablet [Roche], a scoop of DNase I [NEB]) for 5 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed in RIPA buffer or high-salt RIPA buffer supplemented with cOmplete EDTA-Free protease inhibitor (Roche) and PhosSTOP phosphatase inhibitor (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... and finally in 1x low salt buffer without detergents (25 mM Tris pH 8.0, 150mM NaCl, 1x Protease inhibitor cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were washed with Low Salt buffer (50 mM HEPES pH 7.5, 140 mM NaCl, 1% Triton X-100, 1x protease (Roche) and phosphatase inhibitors (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2021Quote: ... brain and dura mater were chopped and subject to enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 2.5 mg/mL collagenase D (Roche) and 0.1 mg/mL DNase I (Roche ...