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Citations for Roche :
651 - 700 of 6729 citations for 7 chloro 5 methylpyrazolo 1 5 A pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... 0.1% NP-40 and 5 mM β-mercaptoethanol) supplemented with fresh protease inhibitors (AEBSF, Complete™ EDTA-free Protease Inhibitor Cocktail, Roche). Emulsions were snap-frozen in droplets in liquid nitrogen and cells subjected to cryogenic grinding using a Ball Mill MM 400 (5 cycles of 3 minutes at 20 Hz) ...
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bioRxiv - Microbiology 2020Quote: ... G355-5 cells were transfected with 1 to 6 µg DNA in 6 cm dishes using X-treameGENE 9 (Roche, Basel, Switzerland). The 293T/17 cell line was obtained from ATCC (CRL-11268 ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% NP-40 and 5 mM β-mercaptoethanol) in the presence of protease inhibitors (Complete™ EDTA-free Protease Inhibitor Cocktail, Roche). Suspensions were flash frozen in droplets and cells subjected to cryogenic grinding using a Ball Mill MM 400 (5 cycles of 3 minutes at 20 Hz) ...
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bioRxiv - Plant Biology 2021Quote: Frozen inflorescence tissue was ground with liquid nitrogen and resuspended in lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5 mM DTT, 1mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were boiled at 95°C for 5 minutes and diluted 1:1000 in dilution buffer provided in ATP luminescence kit HSII (Roche, Cat# 11699709001). The further assay was performed as instructed by the kit’s manual in Luminometer (Promega GloMax96 Microplate Luminometer) ...
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bioRxiv - Molecular Biology 2022Quote: ... ESCs or HEK293T cells were harvested and lysed in TEN buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% Na-Deoxycholate, supplement with Roche cOmplete Protease Inhibitor). The lysates were quantified by the Bradford method and equal amount of proteins were loaded for Western blot assay ...
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bioRxiv - Microbiology 2023Quote: ... Vero/TMPRSS2 cells (JCRB Cell Bank, Cat# JCRB1818) were cultured in DMEM containing 5% FBS and 1 mg/mL G418 (Roche, Basel, Switzerland). VeroE6/TMPRSS2 cells (JCRB Cell Bank ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were brought to room temperature and washed twice in TNT before being incubated in blocking buffer (TNT+5% sheep serum+1% Roche blocking reagent) for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was resuspended in Buffer B (10 mM Pipes, 50 mM KCl, 5 mM MgCl2, 1 mM DTT, 2 mM PMSF, Roche protease inhibitor) with 1% Triton X-100 and incubated on ice for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... in 5% non-fat dry milk in TBS/0.1% Tween-20 (TBST) or in 1% Western blocking reagent (WBR) (Roche Applied Science, Germany) in TBS (for β-actin) ...
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bioRxiv - Microbiology 2023Quote: ... Each membrane was incubated with rat anti-HA primary antibody (Sigma 11867423001; clone 3F10, monoclonal from Roche, 1:5000 in 5% milk) overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... or 48 h after seeding in a Triton X-100-based buffer (150 mM NaCl, 20 mM Tris-HCl pH 7.5, 5 mM EDTA, 1% Triton X-100, Roche protease inhibitor cocktail). A linear sucrose density gradient was prepared in 13x51 mm centrifuge tubes (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was isolated by isopropanol precipitation of cells and tumor lysates (lysis buffer was Tris [pH 8] 100 mM, EDTA 5 mM, SDS 0.2%, NaCl 200 mM, and 1 mg/mL proteinase K [Roche Diagnostics, Mannheim, Germany]) and resuspended in Tris/EDTA (TE ...
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bioRxiv - Developmental Biology 2021Quote: ... 5% glycerol and 140 mM NaCl) supplemented with Protease Inhibitor Cocktail (PIC, Roche) on ice for 10 min ...
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bioRxiv - Developmental Biology 2020Quote: ... blocking was in 5% Horse Serum (X?) and 0.5% Western Blocking Reagent (Roche) MABTween solution and anti-DIG-POD was used ...
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bioRxiv - Immunology 2019Quote: ... 5% glycerol) containing Complete® protease inhibitor and Phosphostop® phosphatase inhibitors (Roche). Immunoprecipitation was then conducted by first preclearing lysates with protein A/G-Sephrose (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2019Quote: ... 5 min each with 2x SSC (Roche-Apply science 11 666 681 001) by submerging in a tray on a rotating platform ...
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bioRxiv - Genomics 2019Quote: ... 62°C for 5 min) using KAPA HiFi HotStart ready mix (KAPA Biosystems) and NextFlex primer mix (Bio Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... 5 mM mercaptoethanol) with a tablet of cOmplete™ Protease Inhibitor Cocktail (Roche). The suspension was sonicated (10 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% glycerol and 140 mM NaCl) supplemented with Protease Inhibitor Cocktail (PIC, Roche) on ice for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... 5 μM DTT) containing protease inhibitor (Complete Mini, EDTA-free; Roche Applied Science), 10 U/ml benzonase Nuclease (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Reaction mix contained 5 μl of buffer I (Expand Long Template, Roche, Germany), 3% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... and then stained with 5 μl Annexin V and 5μl PI (Roche, Germany) for 20 min and analyzed with Beckman CytoFLEX flow cytometry ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol and 2 mM Dithiothreitol (DTT)) supplemented with protease inhibitor cocktail (Roche) and lysed by sonication on ice ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol and 10 mM imidazole supplemented with EDTA-free protease inhibitor (Roche). The cell suspension was lysed by ultrasonication and the lysate was cleared by centrifugation at 40,000 x g for 40 min ...
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bioRxiv - Cell Biology 2022Quote: ... the 5-Bromo-2’-deoxy-uridine Labelling and Detection Kit II (Roche, UK) was used per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The 5’ and 3’ linkers were terminally labelled with digoxigenin-11-dUTP (Roche) or biotin-16-dUTP (Roche) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 5 μL of 5X Kapa Buffer (Kapa Biosystems, Inc., A Roche Company, Canada), 0.75 μL of 10 mM dNTPs ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 5 μL of 5X Kapa Buffer (Kapa Biosystems, Inc., A Roche Company, Canada), 0.75 μL of 10 mM dNTPs ...
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bioRxiv - Neuroscience 2020Quote: ... all membranes were blocked with 5% Western Blocking Reagent (Roche, Indianapolis, IN, USA) in TBST ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the following were mixed: 5 μL Kapa HiFi buffer 5X (Kapa Biosystems, USA), 0.75 μL dNTPs 10 μM ...
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bioRxiv - Immunology 2021Quote: ... and 5 mM Na3VO4) in the presence of complete Protease Inhibitor Cocktail (Roche). The cell lysates were incubated on ice for 30 min and were then centrifuged for 20 min at 15,000 rpm at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mM β-mercapto-ethanol) supplemented with 5 μg/ml DNase I (Roche) and lysed using a homogenizer (Avestin Emulsiflex C5 ...
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bioRxiv - Biochemistry 2021Quote: ... 5% glycerol and 10 mM imidazole supplemented with EDTA-free protease inhibitor (Roche). The cell suspension was lysed by ultrasonication and the lysate was cleared by centrifugation at 40,000 x g for 40 min ...
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bioRxiv - Microbiology 2021Quote: ... based on the 5′ and 3′ RACE kit (2nd generation, Roche, Basel, Switzerland). Viral nucleic acids were extracted from 140 μl of the virus transport medium derived from the nasopharyngeal swab using the viral RNA isolation kit according to the manufacturer’s protocol (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... Cytokines were added at the following concentrations: GM-CSF (5 ng/mL, Roche), TNF (10 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... 5) Incubated with 50μL of TUNEL reaction mixture for one hour (Roche protocol); 6 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5% glycerol) containing a protease inhibitor cocktail (Roche,11 697 498 001). The cell lysates were further sonicated on ice for 10 seconds ...
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bioRxiv - Microbiology 2022Quote: ... and blocked at room temperature with either 5% bovine serum albumin (BSA, Roche), 5% milk or Odyssey buffer (1:1 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... determined as the amount of 5-bromo-2′-deoxy-uridine (BrdU) incorporation (Roche BrDU Labeling and Detection Kit II ...
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bioRxiv - Genetics 2023Quote: ... 5 mM Tris-HCl (pH 7.4) supplemented with Protease Inhibitor cocktail (Roche, #04693159001) and transferred to a 7 mL Dounce tissue grinder (KIMBLE KONTES ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 3 minutes 10 μL of 5 mg/mL Liberase (Roche, cat # 05401119001) were added ...
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bioRxiv - Developmental Biology 2023Quote: ... using a 5 μl reaction mixture of DNA SYBR Green I Master (Roche) according to the standard manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 5 mM beta-mercaptoethanol (BME)) with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). After lysate centrifugation at 48.384g for 45 min at 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The cell proliferation ELISA BrdU (5′-bromo-2′-deoxyuridine) colorimetric assay (Roche,11647229001) was performed as per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... then 5 min at 62°C) with KAPA HiFi HotStart Ready Mix (Roche). Libraries were quantified by Bioanalyzer (Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... 5% glycerol) containing a protease inhibitor cocktail tablet (Complete EDTA-free, Roche Diagnostics) and gently stirred for 30 min in the presence of 0.2 mg/mL lysozyme (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 5% glycerol) supplemented with Complete Mini protease inhibitor mixture tablet (Roche Applied Science) and 10 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Genetics 2023Quote: ... Each reaction consisted of 5 μL LightCycler 480 SYBR Green Master mix (Roche), 0.5 μL each of the forward and reverse primers (100 μM ...
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bioRxiv - Immunology 2024Quote: ... Cell nuclei were stained with 5 µg/ml of DAPI (10236276001, Roche Diagnostics).