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Citations for Roche :
501 - 550 of 6729 citations for 7 chloro 5 methylpyrazolo 1 5 A pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 % DMSO and 12.5 μL of KAPA HiFi HotStart ReadyMix (Roche). The primers used to amplify the library were P1 (5’-GGGGAAGCTTGCCGTAAG-3’ ...
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bioRxiv - Neuroscience 2021Quote: ... and .5 mM spermidine along with phosphatase and protease inhibitors (Roche). All steps were performed on ice ...
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bioRxiv - Neuroscience 2021Quote: ... and 5’-GCTTGAGGTAGC CCTGTTGTCACC-3’ using KAPA HiFi Hotstart polymerase (Roche:KK2602). This PCR reaction produced a 185 bp wild type band and a 750 bp knockout band in heterozygous animals ...
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bioRxiv - Bioengineering 2021Quote: ... 5 μl 2500 U/ml DNase I (04716728001 Roche, Sigma Aldrich) was added to the single-cell suspension 10 min before the end of the digestion ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 mM CaCl2 and 15 units of S7 micrococcal nuclease (Roche). Lysates were sonicated for 10 seconds at low power followed by incubation on ice for 30 minutes and clarification by centrifugation at 13,000 x g for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... ATP 5 mM) supplemented with proteases inhibitors (cOmplete EDTA-free, Roche). Cells were homogenized in a ball homogenizer with 10 μm clearance ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% (w/v) glycerol) with protease inhibitor tablet (EDTA-free, Roche), and lysed by sonication ...
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bioRxiv - Cell Biology 2021Quote: ... and digested with collagenase D (5 mg/mL, #11088882001, Roche, Germany) and dispase (2 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... pastoris were removed by incubation with 5 mU of EndoglycosidaseH (Roche) in 50 mM sodium citrate buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... 5–10 mM NaF) containing a protease inhibitor cocktail (Roche, 04693132001) and the protein lysis buffers were incubated on ice for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... PCR conditions were as follows: 5 μl Kapa Enhancer (Kapa Biosystems), 5 μl Kapa Buffer A ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 2) 5 10^6 copies of bacteriophage MS2 RNA (Roche) were spiked in per isolation ...
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bioRxiv - Neuroscience 2019Quote: ... 5 mM DTT and protease inhibitors (#04 693 159 001 Roche). After a squeezing step ...
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bioRxiv - Genomics 2019Quote: ... 0.08U KAPA2G Robust HotStart DNA Polymerase (5 U/μL, Roche KK5517). PCR reactions were performed using a thermocycler with the following conditions ...
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bioRxiv - Neuroscience 2020Quote: ... The PVDF membranes were blocked in 5% Western Blocking Reagent (Roche) then probed with primary and secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... 5 μl FastStart Universal SYBR Green Master mix (Roche, Basel, Switzerland) and 3 μl MilliQ ...
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bioRxiv - Neuroscience 2020Quote: ... 5 mM magnesium acetate) supplemented with protease/phosphatase inhibitor cocktail (Roche) and RNasin Plus (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... 5% (w/v) glycerol] supplemented with complete protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail (Sigma ...
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bioRxiv - Immunology 2022Quote: ... samples were incubated in 5 mM 1,4-dithiothreitol (Roche, Basel, Switzerland) for 20 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM Benzamidine and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche)) was added and mixed by pipetting ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μl of 10X 5-Bromo-2’-deoxyuridine (BrdU) (Roche, Germany) per well was added ...
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bioRxiv - Plant Biology 2023Quote: ... 5 mM Benzamidine and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche)) and incubated on ice for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM β-mercaptoethanol and protease inhibitors (cOmplete, Roche, Basel, Switzerland) and lysed mechanically using EmulsiFlex (Avestin ...
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bioRxiv - Microbiology 2023Quote: ... 5 mM β-ME) with complete EDTA-free protease inhibitor (Roche). The cells were lysed by sonication (Vibra-Cell™ VCX 130 ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM Na-molybdate and EDTA-free protease inhibitor cocktail (Roche). Protein concentration was measured using the Bradford method (Bio-Rad # 500-0006) ...
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bioRxiv - Cell Biology 2022Quote: ... Aliquots were incubated with anti-GFP (5 µg, Roche, # 11814460001, Germany) for GFP or HA11(5ug ...
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bioRxiv - Biophysics 2023Quote: ... 5 % glycerol and cOmplete™ EDTA-free protease inhibitor cocktail (Roche).
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bioRxiv - Cell Biology 2022Quote: ... 5 mM Na-molybdate and EDTA-free protease inhibitor cocktail (Roche). Protein concentration was measured using the Bradford method (Bio-Rad # 500-0006) ...
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bioRxiv - Cell Biology 2023Quote: ... and 5% IGEPAL CA-630 supplemented with cOmplete protease inhibitors (Roche), and incubated on ice for 15 mins ...
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bioRxiv - Cell Biology 2023Quote: ... 5 units/mL Benzonase and an EDTA-free protease inhibitor (Roche). After sonication ...
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bioRxiv - Biochemistry 2023Quote: ... 5% glycerol supplemented with DNase1 and 1xEDTA-free protease inhibitors (Roche)) ...
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bioRxiv - Developmental Biology 2024Quote: ... 5’-AGCGTTCACATCATATGGCA-3’) using Taq DNA polymerase (Roche, Cat. No. 11146165001). Fragments of foxl2l and id1 were cloned into pGEMT-easy plasmid by TA cloning while nanos2 fragment was cloned into pCS2+ plasmid by BamHI and KpnI ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM DTT) supplemented with EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication for ten cycles of 30 pulses of sonication and 30 s breaks using a Hielscher UP200st set to pulse=70% ...
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bioRxiv - Bioengineering 2024Quote: ... with EDTA (invitrogen, #15575-038) (5 mM) and pronase (Roche, #10165921001) (1 mg/mL) ...
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bioRxiv - Neuroscience 2024Quote: ... 5 µM MG132) and a protease inhibitor cocktail (Roche, Basel, Switzerland), as previously described72 ...
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bioRxiv - Neuroscience 2020Quote: ... for 30 min at 4°C on a rotator and S1 (1 ml for cerebellum and 1 ml for cerebrum) was incubated with 5 μg of anti-HA mAb (Roche, clone 12CA5, RRID: AB_514505) at 4°C for 60 min on a rotator ...
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bioRxiv - Developmental Biology 2019Quote: ... and subsequently incubated in antibody solution (5% Sheep serum, 1% Tween20 and 1:2500 dilution of Alkaline Phosphatase-linked α-Digoxigenin antibody, Roche, 11093274910 in TBST) for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 100 mL of cold lysis buffer (50 mM HEPES pH 8, 175 mM NaCl, 5 % glycerol, 1 mM EDTA and 2 tablets of protease inhibitors by Roche). The bacterial solution was then incubated at room temperature (RT ...
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bioRxiv - Developmental Biology 2020Quote: ... and 0.1% Tween 20) for 5 min each and then incubated in the AP reaction buffer with NBT/BCIP substrate (Roche).
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... Eight million HEK293T cells were lysed with 800 μL cell lysis buffer (HBS, 0.1% NP-40, 5 mM EGTA, supplemented with 1 χ protease inhibitor cocktail [Roche, Cat.# 11873580001] ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear pellets were lysed by sonication on ice in 10 mL lysis buffer (50 mM Tris pH 7.4, 0.5 M NaCl, 0.4% SDS, 5 mM EDTA, 1 mM DTT, 1x Roche complete protease inhibitor). After sonication ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and EDTA-free protease inhibitors (Roche). The lysate was clarified by centrifugation and the proteins in the supernatant were purified by gravity Ni-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Cancer Biology 2019Quote: Whole cell lysates from 5.0 × 106 cells were prepared using SDS-based buffer (5 mM EDTA, 1% SDS) supplemented with Protease inhibitor cocktail (Roche) and PhosSTOP EASY (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested after 48-72 h and resuspended in lysis buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 30 mM imidazole, 1 mM TCEP and complete EDTA-free protease inhibitors cocktail [Roche]) and lysed by sonication ...
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bioRxiv - Bioengineering 2021Quote: ... S-phase Synchronous HeLa S3 cells were washed with ice-cold 1× PBS and lysed in a swelling buffer (20 mM HEPES, pH 7.5, 2 mM MgCl2, 5 mM KCl, 1 mM Dithiothreitol [DTT], and protease inhibitor cocktail [Roche; #11836170001]) supplemented with energy-regenerating mixture ...
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bioRxiv - Neuroscience 2019Quote: ... and hybridized overnight at 55°C with 1 ng/ml digoxigenin-labelled (GGGGCC)5 oligonucleotide probe (Integrated DNA Technologies) in 25 ml hybridization reaction (EasyHyb Granules, Roche). Following denaturation of the probe at 95°C for 5 min ...
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bioRxiv - Biochemistry 2021Quote: Cells were washed 2x with PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1X PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
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bioRxiv - Genomics 2021Quote: ... Cells from two biological replicates were lysed with ChIP lysis buffer (5 mM PIPES pH8, 85 mM KCl, 1% Igepal) with a protease inhibitor (PI) cocktail (Roche). Nuclei were collected by centrifugation at 2,000 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... un-probed input RNA was also subjected to Sanger sequencing: RNA was dissolved in 5 μl water and supplemented with 1 μl of 10 mM corresponding ddNTP (Roche), then reverse-transcribed into cDNA and analysed by PAGE as described below.