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Citations for Roche :
151 - 200 of 2640 citations for 7 9 Diazaspiro 4.5 decane 6 8 10 trione 7 ethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 4.5 mg of psPAX2 and 1 mg of pVSVG with X-tremeGENE HP (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: Pancreases were rapidly dissected after intra-ductal injection from 9-10 week-old male mice and islets were isolated by digesting the pancreas with collagenase P (Roche, Basel, Switzerland) and purified using a Ficoll gradient (Merck Biochrom ...
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bioRxiv - Cancer Biology 2020Quote: ... Resulting cell pellets were resuspended in Buffer A (100 mM NaH2PO4, 10 mM Tris, 6 M GuHCl, 10 mM imidazole) + PIC (Roche 05056489001). Cells were sonicated 3X at 25% power for 10s (Cole-Parmer GE 130PB-1) ...
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bioRxiv - Cell Biology 2020Quote: ... X-tremeGENE 9 and Complete Protease Cocktail from Roche; Alexa 488 ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were transfected using either X-tremeGENE 9 (Roche), polyethylenimine (Polysciences ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were cotransfected using XtremeGENE 9™ (Roche) using the above CRISPR plasmid ...
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bioRxiv - Developmental Biology 2020Quote: ... The transfection reagent XtremeGENE 9 was purchased from Roche Molecular Systems and polybrene from MilliporeSigma ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... and GFP- tagged pCREB3L3 using X-tremeGENE 9 (Roche). Cells were grown on coverslips ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... Indicated plasmids were transfected with X-tremeGENE 9 (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 12 μL X-tremeGENE 9 DNA Transfection Reagent (Roche) and 500 μL Opti-MEM (Gibco ...
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bioRxiv - Immunology 2019Quote: ... into HEK293T cells using X-tremeGENE™ 9 (Roche) as per the manufacturer’s instructions41 ...
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bioRxiv - Cell Biology 2019Quote: ... or pRetroX-Tet-On using X-tremeGENE 9 (Roche).
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bioRxiv - Microbiology 2020Quote: ... the cells were transfected using X-TremeGENE 9 (Roche) with the pLentiCRISPR plasmids and the lentiviral packaging plasmids pMD2.G and psPAX2 to generate lentiviral particles coated with the VSV-G protein and incubated overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... 24 μL X-tremeGENE 9 DNA transfection reagent (Roche) and 500 μL Opti-MEM media (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... Lentivirus was generated by using Xtremegene-9 (Roche 06365787001) to co-transfect the scaffold-containing pLenti plasmid ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 mM Tris pH 8) containing protease inhibitors (Roche mini EDTA-free, 1 tablet in 10 ml) and phosphatase inhibitors (2mM Sodium Ortho-Vanadate ...
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bioRxiv - Microbiology 2022Quote: ... and lentivirus vectors at a 2:8:10 μg ratio to HEK293T cells using Xtreme-Gene9 (Roche) and lentivirus-containing supernatants were collected 72hr post-transfection ...
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bioRxiv - Genomics 2022Quote: ... 1 μl 10 μM reverse primer with different 6-nt index sequence (barcode) and 10 μl 2X KAPA HiFi HotStart ReadyMix (KAPA Biosystems, KK2602), was performed using 95 °C ...
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bioRxiv - Genetics 2020Quote: ... 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml, ½ Roche phosSTOP tablet/10 ml) using 14 × 1.5 min bursts on a BioSpec mini bead-beater at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... or X-Treme transgene 9 transfection reagent (Roche, Basel, Switzerland), according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected using X-tremeGENE 9 transfection reagent (Roche). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... cell line using X-tremeGENE 9 DNA transfection reagent (Roche). 48 h after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... using X-tremeGENE 9 DNA transfection reagent (Roche, cat# 6365779001) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and transfected 24 h later using Xtreme-GENE 9 (Roche) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were seeded and transfected using XtrememGene 9 (Roche, USA) according to manufacturer’s manual 48 h prior to infection ...
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bioRxiv - Cell Biology 2021Quote: ... pX330-based plasmids were transfected using X-tremeGENE-9 (Roche) together with a mCherry-expressing plasmid ...
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bioRxiv - Cell Biology 2022Quote: ... containing AAVS1-targeting recombination arms [62] using XtremeGene 9 (Roche). Transfected cells were allowed to recover for 48 hrs before treatment with 1 μg/ml puromycin (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... 24 h) using X-tremeGENE 9 DNA Transfection Reagent (Roche) (3:1 ratio of reagent to DNA ...
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bioRxiv - Cell Biology 2024Quote: ... media containing 24 μL of X-tremeGENE 9 DNA (Roche) and added to HEK cells ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.4 µg vsFULL envelope plasmid using Xtremegene-9 (Roche). After 16 hours ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma], 0.2 % Nonidet P-40 substitute [Sigma], 1x Complete EDTA-free Protease inhibitor [Roche]) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... beads containing bound methylated DNA were resuspended in 250 μl digestion buffer (50 mM Tris pH 8, 10 mM EDTA, 0.5% SDS) with 100 μg Proteinase K (Roche) and incubated at 65°C for 4 h ...
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bioRxiv - Molecular Biology 2021Quote: ... washed in cold PBS and incubated on ice in cold swelling buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) for 10 min ...
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bioRxiv - Immunology 2021Quote: ... Pellets of cross-linked cells were resuspended in shearing buffer (10 mM Tris-HCl pH 8, 0.1% SDS, 1 mM EDTA) supplemented with Complete Protease Inhibitor Cocktail (Roche), and chromatin was sheared to an average fragment size of 200-250 bp ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were removed from neurons cultured in MatTek dishes at 8-10 days in vitro (DIV) by incubating cells with extraction buffer (1M sucrose, 0.15% Triton X-100 (Roche, Basel ...
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bioRxiv - Molecular Biology 2020Quote: ... Released nuclei were washed (10 mM Tris-HCl pH 8, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x PIC (Roche)) for 5 minutes at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Isolated nuclei were then lysed with lysis buffer (50mM Tris-HCl, pH 8.1, 10 mM EDTA, pH 8, 1% SDS, 1X protease inhibitor cOmplete EDTA-free cocktail, Roche). Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...
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bioRxiv - Immunology 2021Quote: ... Add 120 ul of fresh ChIP SDS Lysis Buffer (0.5% SDS, 50 mM Tris, pH 8, 10 mM EDTA, 1 × protease inhibitor cocktail (Roche)) per 5 million cells ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl GTEN-buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl,1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Plant Biology 2023Quote: ... A GTEN-based buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl, 1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Biochemistry 2023Quote: ... washed with 1 x PBS and resuspended in 100 μl of SoTE buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.15 % Triton X-100, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2024Quote: Ni-NTA Magnetic Agarose Beads were incubated with 1.5µg of recombinant His-TIRR(8) in binding buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2, 10% Glycerol, Protease inhibitors (EDTA free, Roche)) for 1 hour at 4°C on a wheel ...
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bioRxiv - Genomics 2024Quote: ... the embryos were transferred to pre-hybridization buffer (50% deionized formamide, 5x SSC pH 4.5, 2% Roche Blocking Reagent ...
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bioRxiv - Cell Biology 2022Quote: Cells were transfected using X-tremeGENE 9 DNA Transfection Reagent (Roche). To generate retroviral particles ...
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Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in developmentbioRxiv - Genomics 2020Quote: ... The plasmid was transfected into H1 hESCs with XtremeGene 9 (Roche). 24 hours later ...
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bioRxiv - Genomics 2020Quote: ... The plasmid was transfected into H1 hESCs with XtremeGene 9 (Roche), and 24 hours later 8000 GFP+ cells were sorted into a well of six-well plate ...
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bioRxiv - Genomics 2019Quote: ... The plasmid was transfected into H1 hESCs with XtremeGene 9 (Roche). 24 hours later ...
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bioRxiv - Biophysics 2021Quote: ... and cells were transfected using the Xtreme-Gene 9 reagent (Roche) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... Transfections were performed using X-tremeGENE 9 DNA transfection reagent (Roche), with 1.2 µl X-tremeGENE reagent per 500 µg DNA reaction ...
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bioRxiv - Cancer Biology 2022Quote: ... using X-treme GENE 9 DNA transfection reagent (Roche, XTG9-RO) to produce the lentiviral particles ...