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Citations for Roche :
51 - 100 of 1648 citations for 6 Amino 5 formamido uracil since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 16ng bisulfite converted DNA was obtained and amplified via KAPA HiFi Uracil+ Kit (Roche 7959052001) in a total volume of 25uL ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM amino acids (Roche), 1 nM pY71sfGFP plasmid encoding superfolder GFP (M ...
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bioRxiv - Microbiology 2020Quote: ... G355-5 cells were transfected with 1 to 6 µg DNA in 6 cm dishes using X-treameGENE 9 (Roche, Basel, Switzerland). The 293T/17 cell line was obtained from ATCC (CRL-11268 ...
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bioRxiv - Developmental Biology 2021Quote: ... Library amplification and indexing were performed with KAPA HiFi HotStart Uracil+ ReadyMix (2×) (Kapa Biosystems KK2801). The PCR amplification was carried out as follows ...
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bioRxiv - Cell Biology 2022Quote: ... The bisulfite converted libraries were amplified for 12 cycles with KAPA HiFi HotStart Uracil+ ReadyMix (Roche). The amplified libraries were cleaned up with AMPure beads (0.8X ratio ...
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bioRxiv - Biochemistry 2020Quote: ... and then amplified for 10 cycles using Kapa HiFi HotStart Uracil ReadyMix (Kapa Biosystems, KK 2801) before sequencing on a Hiseq 4000 (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... except the Enhanced PCR Mix (EPM) was substituted with KAPA HiFi HotStart Uracil+ ReadyMix (Kapa Biosystems) to enable the amplification of uracil encountered in the DNA template ...
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bioRxiv - Genomics 2021Quote: ... Library amplification (13 PCR cycles) was performed with KAPA HiFi HotStart Uracil+ DNA polymerase (Kapa Biosystems). Library size was determined by the Agilent 4200 Tapestation system ...
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bioRxiv - Genetics 2021Quote: ... The library PCR reactions for the bone samples utilized KAPA HiFi HotStart Uracil+ ReadyMix (Roche Sequencing) to accommodate for uracils present in the native DNA molecule from cytosine deamination ...
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bioRxiv - Genomics 2021Quote: ... the captured strands were process for library amplification by PCR using KAPA Uracil+ HiFi enzyme (Roche) and TrueSeq primers included in the kit ...
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bioRxiv - Genomics 2021Quote: ... The resulting libraries were amplified with 4 PCR cycles using KAPA HiFi Uracil+ PCR enzyme (Roche). Libraries were quality controlled on a TapeStation 2200 HSD1000.
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bioRxiv - Genomics 2024Quote: ... treated with USER enzyme and amplified by PCR using HiFi HotStart Uracil+ Ready Mix (Kapa/Roche). Completed libraries were quantified by qPCR using Kapa Library Quantification kit (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... for 4hrs and amplified with index primers using KAPA HiFi HotStart Uracil+ Ready Mix (KAPA Biosystems). The samples after PCR amplification were then purified using Ampure XP Beads (Beckman Coulter ...
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bioRxiv - Genetics 2024Quote: ... and PCR amplified using the KAPA HiFi Uracil PCR Kit (Catalogue Number: ROC-07959052001, Kapa Biosystems). The final libraries were assessed with the Agilent 2200 Tapestation System using D1000 Kit (Catalogue Number:5067-5582) ...
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bioRxiv - Genomics 2020Quote: ... DNA was amplified by 9 cycles of PCR using the Kapa Hifi Uracil + DNA polymerase (KAPA Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... PCR amplification with 9 – 12 cycles using the KAPA HiFi HotStart Uracil + DNA Polymerase (Roche/KAPA Biosystems) was performed according to suggested protocols ...
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bioRxiv - Genomics 2022Quote: ... PCR amplification with 9 – 12 cycles using the KAPA HiFi HotStart Uracil + DNA Polymerase (Roche/KAPA Biosystems) was performed according to suggested protocols ...
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bioRxiv - Genetics 2023Quote: ... We PCR-amplified bisulfite-treated DNA with 15-16 cycles with KAPA HiFi Uracil polymerase (Kapa Biosystems). We used Illumina TruSeq unique index adapters for PCR amplification and multiplexing of the libraries ...
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bioRxiv - Genomics 2023Quote: ... Whole genome amplification was performed by addition of 20 μL 2X KAPA HiFi HotStart Uracil+ ReadyMix (Roche KK2801), 0.4 μL 100 μM Nextera indexed P5 primer and 0.4 μL 100 μM Nextera indexed P7 primer (SI Appendix ...
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bioRxiv - Genomics 2023Quote: ... Whole genome amplification was performed by addition of 20 μL 2× KAPA HiFi HotStart Uracil+ ReadyMix (Roche KK2801), 0.4 μL 100 μM Nextera P5 index primer and 0.4 μL 100 μM Nextera P7 index primer and incubation at 98°C for 45 s ...
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bioRxiv - Molecular Biology 2020Quote: ... For the qPCR we used a 25 µL reaction with 12.5µl of 2x KAPA HiFi Uracil+ hot start polymerase mix (Roche # KK2800), 1.25µl of 10 µM forward and reverse PCR primers (IDT) ...
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bioRxiv - Genomics 2020Quote: ... Library fragments were then subjected to 7 cycles of PCR amplification with KAPA HiFi Uracil+ DNA polymerase (KAPA Biosystems). Single-end 100 bp sequencing was performed on a HiSeq 1500 or a MiSeq (Illumina).
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bioRxiv - Genomics 2020Quote: ... Bisulfite converted DNA was then used as template for PCR amplification with KAPA HiFi HotStart Uracil kit (Roche, KK2801) using primers surrounding Site 1 in intron 8 of the Fto gene ...
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bioRxiv - Genomics 2019Quote: All 5’ fragments were amplified off the array using HSSF-ATGC and DO_15R_PU (Supplemental Table 8) with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) and stopped before plateauing ...
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bioRxiv - Immunology 2020Quote: ... brains were cut into 6 pieces and incubated in 5 mL HBSS containing 50U/mL DNase (Roche), and 4U/mL papain (Worthington-Biochem ...
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bioRxiv - Molecular Biology 2020Quote: ... We then used four times fewer cycles than indicated by the qPCR to lead to logarithmic phase of amplification and prepared a PCR reaction of 50 µl PCR master mix consisting of 25µl of 2x KAPA HiFi Uracil+ hot start polymerase (Roche # KK2800) mix ...
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bioRxiv - Genomics 2020Quote: ... Entire material of one conversion was equally distributed to a 96-well plate and amplified via PCR using KAPA HiFi Uracil+ Kit (Roche) in a total volume of 16µl ...
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bioRxiv - Molecular Biology 2020Quote: ... the USER digestion product was directly performed index PCR with 1 x KAPA HiFi HotStart Uracil+ ReadyMix (Kapa Biosystems, Cat.No.KK2801). Protocol of Strand Test 3 was almost same as Strand Test 2 ...
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bioRxiv - Genomics 2021Quote: ... The enriched libraries were amplified by a second round of LM-PCR with 16 cycles using Kapa Hifi Uracil+ polymerase (Roche) and purified with AMPure XP magnetic beads ...
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bioRxiv - Molecular Biology 2021Quote: ... The bisulfite-treated DNA was amplified by nested PCR (Supplementary Table 2) using KAPA HiFi HS Uracil+ ReadyMix (Kapa Biosystems). In the first and second rounds of PCR ...
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bioRxiv - Genomics 2021Quote: Bisulfite converted DNA was amplified and barcoded in 50 µL PCR reaction (22 µL bisulfite-converted DNA, 25 µL 2× KAPA HiFi HotStart Uracil+ ReadyMix (Roche), 1.5 µL 10 µM i5 universal PCR primer ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR was performed with 500 nM of oligo U1 and U2 primer in 2x KAPA HiFi HotStart Uracil+ ReadyMix (Roche) in 50 μl ...
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bioRxiv - Genomics 2023Quote: ... Bisulfite converted DNA library was amplified using truncated TruSeq™–Compatible Indexing Primer and KAPA HiFi Uracil+ ReadyMix (Roche, KK2801), supplemented with MgCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Converted DNA was used as a substrate for PCR amplification of the below endogenous CTCF sites using bisulfite-compatible primers and KAPA HiFi Uracil+ (Roche) ([95 °C ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-SNX15 MIT was bound to cOmplete His-Tag purification beads (5 mL, Roche, Germany, 2h) and washed with 2 L wash buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... and the whole converted DNA was used as input for library preparation using the Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences) according to the recommended protocol and indexing PCR using 2X KAPA HiFi HotStart Uracil+ ReadyMix (KAPA Biosystems). Indexed ...
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bioRxiv - Genomics 2019Quote: ... Quality control for primer target amplification and performance in multiplex PCR was assessed by screening each primer pair in singleplex reactions using gDNA from PC3 or DU145 cells (10 ng) as template (see below) using the HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems). Reactions were supplemented with uracil N-glycosylase (0.2 U ...
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bioRxiv - Molecular Biology 2020Quote: ... A-tailed and ligated to an adaptor in the form of a uracil-containing stem-loop using the KAPA HTP Library Preparation Kit PCR Free (KAPA Biosystems). Lambda exonuclease (New England Biolabs ...
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bioRxiv - Genomics 2019Quote: All 192 bp enhancers were amplified from the array using HSSF-ATGC and HSS-R-clon (Supplemental Table 8) with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) with SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: All libraries were amplified off the array using the primers indicated in Supplemental Table 10 with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) as described above ...
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bioRxiv - Immunology 2021Quote: ... the VH or VL primer mix (reported in Table S5, final concentration of 0.5 µM each) and Kapa HiFi HotStart Uracil+ Ready Mix (Kapa Biosystems KK2802) or Kapa HiFi HotStart Ready Mix (Kapa Biosystems KK2601 ...
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bioRxiv - Genomics 2023Quote: The 19.5 μl eluate was placed in one microwell of a 96-well plate preloaded with a PCR reaction mixture containing 25 μl of 2x KAPA HiFi HotStart Uracil+ ReadyMix (Roche, KK2801), 1.5 μl of 25 μM N5XX primer (ordered from IDT with the sequence shown in Supplementary Fig ...
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bioRxiv - Genomics 2023Quote: The 19.5 μl eluate was placed in a 0.2 ml PCR tube preloaded with a PCR reaction mixture containing 25 μl of 2x KAPA HiFi HotStart Uracil+ ReadyMix (Roche, KK2801), 1.5 μl of 25 μM P5 transposon primer (ordered from IDT with the sequence shown in Supplementary Fig ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each eluate (3 µl) was subjected to PCR amplification in 50-μl reactions containing 1 KAPA HiFi HotStart Uracil+ReadyMix (Kapa Biosystems) and 0.3 μM Dual Index primers of NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Genomics 2024Quote: ... we used bisulphite-converted product as input in PCR reactions and tested an annealing temperature gradient ranging from 55 to 62C for 30 cycles with Kapa Uracil+ HotStart ReadyMix polymerase (Roche, 7959052001). Samples of each PCR product were run on a 2% agarose gel ...
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bioRxiv - Genomics 2020Quote: ... of each 12 μl bisulfite-treated DNA sample was amplified using the KAPA HiFi HS Uracil+ polymerase system (Kapa Biosytems/Roche cat. KM2800) for ~12-15 PCR cycles ...
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bioRxiv - Plant Biology 2022Quote: ... DNA fragments were amplified using the Kapa HiFi Hotstart Uracil+ PCR mix in 14 cycles of PCR (Kapa Biosystems, Wilmington, MA, USA). Bisulfite libraries were sequenced on an Illumina HiSeq3000 instrument (150 bp paired-end reads).
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bioRxiv - Cancer Biology 2023Quote: 15 ng of each patient’s cDNA library were amplified using the KAPA HiFi HotStart Uracil+ ReadyMix 2x (Kapa Biosystems, cat. no. KK2801) with 0.5 µM final concentration of custom-primers (Integrated DNA Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... Converted DNA was amplified for 23 cycles using U1 and U2 primers and KAPA HiFi HotStart Uracil+ DNA polymerase (Roche, Pleasanton, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 72°C for 1 min) including the TruSeq primer mix and the KAPA Hifi Uracil+ Poly-merase Master Mix (Kapa Biosystems, Cat. #KK2801). The PCR product was purified and size controlled by Agilent Technologies 2100 Bioanalyzer (High Sensitivity DNA Chip) ...