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Citations for Roche :
51 - 100 of 3310 citations for 26S Proteasome Non ATPase Regulatory Subunit 10 PSMD10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and blocked in 10% Sheep Serum for 2 hours followed by incubation with an anti-DIG antibody (1:2000) (Roche) in TBST / 1% sheep serum overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were blocked in 10% inactivated sheep serum for 1 h followed by overnight incubation with 1:1000 anti-digoxygenin (DIG) antibody (Roche). The sections were washed in PBT and incubated with NBT/BCIP (Roche ...
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bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26 ...
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bioRxiv - Neuroscience 2023Quote: ... Washes were followed by blocking in 10% heat-inactivated sheep serum for 1-3 hours and incubation in buffer containing sheep antidigoxigenin antibody (Roche) at 1:5000 dilution for 16-20 hours at 4oC ...
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bioRxiv - Plant Biology 2024Quote: ... The immuno-complexes was then analysed on a 10% SDS-PAGE gel using immunoblotting methods with anti-HA antibodies (Anti-HA High Affinity Roche 3F/10 ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were blocked with 5% non-fat milk TBST and probed with primary anti-GFP (Roche, 1:1000) and secondary HRP- conjugated anti-mouse antibody (R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... Non-radioactive antisense RNA probes were generated by in vitro transcription using DIG RNA labeling kit (Roche Diagnostics). For histological analysis sections were stained with Hematoxylin and Eosin according standard protocols.
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bioRxiv - Developmental Biology 2022Quote: ... and 10% sheep serum] and incubated with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche Cat# 11093274910) in MABT/Block at 4°C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant containing the immunoprecipitated protein was resolved in a 10% SDS-PAGE gel and detected by an anti-HA antibody (Roche, 12158167001).
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bioRxiv - Developmental Biology 2022Quote: ... samples from the same stage were pooled and resuspended in DPBS with 0.5% non-acetylated BSA and 0.5U/μl RNAse inhibitor (Roche 3335399001).
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bioRxiv - Cell Biology 2023Quote: ... the membranes were blocked using non-fat dry milk and stained with an anti-GFP primary (Roche; 1:1000) and a goat anti-mouse secondary antibody coupled to horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2023Quote: ... 10% SDS) buffer containing 10% protease inhibitor cocktail (Roche) for 30 min on ice ...
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bioRxiv - Biophysics 2023Quote: ... 1% Triton X-100, 10% glycerol, 10 mM NaH2PO4, 10 mM NaP2O7, 100 μM ZnCl2, 10 mM NaF, 1 Roche protease inhibitor tablet), lysed by sonication and the supernatant isolated by centrifugation ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked with 10% lamb serum in TBST for 1 hour at RT and incubated with alkaline phosphatase conjugated to DIG antibody (Roche, Basel, Switzerland) in TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed to determine relative gene expression in pigmented versus non-pigmented mouse melanocytes using a LightCycler 480 instrument (Roche) with PrimeTime Gene Expression Master Mix (IDT DNA ...
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bioRxiv - Neuroscience 2021Quote: ... for qRT-PCR were intron-spanning to avoid amplification of non-digested genomic DNA fragments and were designed by online Universal ProbeLibrary Assay Design Center (Roche). Standard housekeeping genes (elongation factor 1 ...
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bioRxiv - Cancer Biology 2019Quote: Intestinal tumor and adjacent non-tumor tissue were lysed in KALB lysis buffer supplemented with protease inhibitor and phosphatase inhibitor (Roche) and then homogenized using a QIAGEN TissueLyser II ...
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bioRxiv - Immunology 2022Quote: ... Silent point mutations and non-cleavable point mutants were generated by quick-change PCR using Kapa high-fidelity DNA polymerase (Roche) and all mutations were verified by sequencing.
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Phosphomimic and non-phospho mutants (S265D and S265A) were generated by site- directed mutagenesis using PWO DNA Polymerase (Roche, #11644955001). Primers containing the appropriate mutations were designed with QuickChange® Primer Design Program (Agilent) ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR amplification of the re-purified circular fragments have been performed using View-Point specific primers (Reading Primer: 5’-tacacgacgctcttccgatctAACTCGATTTGGAGCGATC-3’; Non-reading Primer: 5’-actggagttcagacgtgtgctcttccgatctCTGGGACTGCACTTGCTC-3’) using the Expand Long Template PCR System (Roche). Amplicons were purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested in non-denaturing buffer (20 mM Tris, 500 mM NaCl, 20 mM Imidazole and protease inhibitor cocktail (Roche), pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... a 2.3 kb-homologous DNA fragment was cloned into a unique SpeI site of a non-replicative plasmid (pIJ10700) using T4 DNA ligase (Roche). The fragment was amplified using Q5® DNA polymerase (NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... 10% glycerol + 1:10 cOmplete mini protease inhibitor cocktail (Roche)) by incubation on ice followed by sonication (Bioruptor ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissues were washed and the secondary antibodies applied (dilutions listed below) with 4′-6-diamidino-2-phenylindole (DAPI: Cat # 10-236-276-001, Roche Diagnostics, Indianapolis, IN) at 1:1,000 for 1 hour ...
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bioRxiv - Genomics 2024Quote: ... Cell pellets were resuspended in 250 µL ice-cold Hi-C lysis buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% Igepal CA630, 1 tablet/10 mL Roche complete mini EDTA-free protease inhibitor) ...
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bioRxiv - Genetics 2020Quote: N-terminal HA-tagged PRUNE1 overexpression constructs were transfected into PRUNE1 non-expressing HEK293 cells using X-tremeGENE HP DNA (Roche; 06366236001) transfection reagent ...
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bioRxiv - Genomics 2021Quote: ... and then resuspended in Antibody buffer (10 mM HEPES pH 150 mM NaCl, 2 mM spermidine, 2 mM EDTA, 0.1% BSA, and Roche complete EDTA-free protease inhibitor) with primary anti-Pol2S5p antibody (Cell Signaling Technology cat ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of nuclear extract of KDM3A and KDM3B endogenously tagged ESCs (prepared as above) was incubated with 10 µg of HA antibody (Roche, clone 12ca5, catalog#11583816001) for 4 hours rotating at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Antibodies used: Mouse anti-GFP primary antibody (Roche) at 1:3,000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: Cells were plated to ∼50% confluence in 6-well plates and 30pmol of siRNA against CTNNB1 (siCTNNB1) or non-targeting siRNA (siNT) (Dharmacon) were transfected using XtremeGENE™ siRNA Transfection Reagent (Roche, Merck) as per manufacturer’s instructions (for SW620 150pmol siRNA was used) ...
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bioRxiv - Cell Biology 2022Quote: ... in 5% non-fat dry milk in TBS/0.1% Tween-20 (TBST) or in 1% Western blocking reagent (WBR) (Roche Applied Science, Germany) in TBS (for β-actin) ...
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bioRxiv - Immunology 2023Quote: ... and PLN from non-diabetic 6w– 8w female mice were minced and enzymatically dissociated with 1 mg/mL collagenase D (Roche Applied Science) and 25 U/mL DNase I in DMEM for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... with 10 U PNK in 10 µl with 20 U RNasin (Roche). After 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... 10 mM Hepes pH 7.9) containing 10 % proteinase inhibitor cocktail (PIC, Roche) and lysed in RIPA buffer + 1x PIC ...
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bioRxiv - Neuroscience 2020Quote: ... 10 units papain (Roche), 5 mM L-cysteine ...
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bioRxiv - Microbiology 2020Quote: ... 10% protease inhibitor (Roche) were added to lyses buffer before using ...
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bioRxiv - Neuroscience 2022Quote: ... 10 U/µl (Roche) for 15 min at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... 50 mM Tris HCl pH 7.5, 10% glycerol, 10 mM EDTA, 10 mM DTT, 1mM NaF, 1% IGEPAL, 2X protease inhibitor, and 2X Roche phosphatase inhibitor) according to the sample quantity (approximately 200 µl of extraction buffer per 100 mg of ground plant material) ...
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bioRxiv - Microbiology 2021Quote: ... Western-blot antibody detection was used using antibodies from Roche Diagnostics Mannheim Germany (Anti-HA ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies were diluted in antibody dilution buffer (catalog# 5280524001, Roche Diagnostics ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μl of 10 mM dNTPs and 10 units of Transcriptor Reverse Transcriptase (Roche).
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bioRxiv - Plant Biology 2020Quote: ... yeast strains were grown for 2 d on non-selective medium and the β-galactosidase enzymatic activity was determined in an assay using red-β-D-galactopyranoside (CPRG, Roche Applied Science) as substrate (Yeast Protocols Handbook ...
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bioRxiv - Developmental Biology 2019Quote: Approximately 150 μl whole blood was collected from dams via a tail tip bleed at 1 h and 5 h post gavage on E13.5 and 14.5 to measure blood alcohol concentration (BAC; see details for measurement below) and non-fasted blood glucose level using an Accu-Chek Performa glucometer (Roche, Bella Vista, NSW, Australia). At PN30 ...
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bioRxiv - Molecular Biology 2020Quote: Equal amount by mole of non-targeted and targeted RNA-Seq library were mixed based on qPCR-based quantification with Kapa Library Quantification Kit (KAPA BIOSYSTEMS, Wilmington, MA, USA). Sequencing of 150-bp paired-ends using HiSeq was carried out.
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bioRxiv - Developmental Biology 2019Quote: ... POD antibodies (Roche) in western blocking reagent overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... DNase (10 μg/ml, Roche) and dispase II (1.5 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μg/mL insulin (Roche) and penicillin-streptomycin (100 U/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μg/mL insulin (Roche), 200 μM indomethacin ...
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bioRxiv - Immunology 2022Quote: ... 10 μg/ml DNAse (Roche) and 2% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mM each dNTP (Roche), 1.13 mg/ml ultra-pure (non-acetylated ...