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Citations for Roche :
4901 - 4950 of 6311 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Column was washed three times with wash buffer (PBS 2X, Triton X-100 0.1%, imidazole 50 mM, NaCl 500 mM, DTT 1 mM, 1x Complete protease inhibitor [Roche]) before storage in 50% glycerol (0.01 % Triton) ...
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bioRxiv - Developmental Biology 2021Quote: ... treatment for 1 hour at 37°C and cDNAs were amplified with KAPA HiFi Hotstart Ready Mix (Roche, KK2602). PCR reactions were conducted as follow ...
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bioRxiv - Developmental Biology 2021Quote: ... blocked in 1xBM Blocking/PBST at RT for 2 hours and incubated with anti-DIG-POD (1:1,000, 11207733910, Roche) at 4°C overnight ...
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bioRxiv - Genomics 2022Quote: ... 100µl of fly heads/∼107 S2 cells were homogenized in 500µl/1ml lysis buffer (50mM TRIS HCL 7.4, 150mM NaCl, 1mM EDTA, 1% TRITON X100 and protease inhibitor tablets [Roche]). Homogenate was kept on ice for 30 min and then sonicated using a Bioruptor sonication device (Diagenode ...
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bioRxiv - Microbiology 2022Quote: ... comprising 0.5 μL of DNA (40 ng/μL) and 1 μL of LightCycler 480 SYBR Green I Master mix (Roche) containing 0.5 μM PCR primers (Eurogenetec SA) ...
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bioRxiv - Molecular Biology 2021Quote: ... in a total volume of 40 µl to 2.4 pmole trypSL in the presence of 1 mM ATP and 20 U RNasin (Roche) in vaccinia capping buffer ...
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bioRxiv - Molecular Biology 2021Quote: In situ antibody stainings were done as described previously 31 using rat anti-HA (MAb 3F10, 1:20; Roche), rabbit anti-FLAG (M2 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4°C with an established dilution in fresh-prepared sample buffer (1% bovine serum albumin and 0.05% Tween-20 in PBS) supplemented with protease inhibitor (cOmplete, Roche). Detection antibody (R&D Systems ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was resuspended in 5ml lysis buffer (20 mM Tris, 150 mM NaCl, 1% NP40 pH 7.5, plus a tablet of protease inhibitor cocktail from Roche) per mg of cell pellet and incubated at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... washed in cold PBS and incubated on ice in cold swelling buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: Mouse livers were homogenized with a motorized tissue grinder in STM buffer (Tris-HCl pH 7.4, 5 mM MgCl2, 250 mM Sucrose, 1 mM DTT, supplemented with 500 µM PMSF, Protease Inhibitor Cocktail [Roche, Basel ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed in 500 μl lysis buffer (50mMTris-HCl, pH8.0, 100mM NaCl, 10m MEDTA, 5% glycerine, 1% NP- 40 and protease inhibitor cocktail (Roche)) for 40 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), washed once with PBS ...
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bioRxiv - Genomics 2020Quote: ... and mixed with 100 μL Binding Buffer (1% Triton X-100, 0.1% Sodium Deoxycholate, 1x complete protease inhibitor (Roche)) plus 100 μL 0.2 μg/μl chromatin followed by overnight incubation on a rotating platform at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Between 60-80 mg pellets were lysed in 1mL lysis buffer (50mMTris-Cl pH 7.0, 10mM EDTA, 1%SDS, 1mM PMSF, complete protease inhibitor cocktail (Roche), 0.1U/mL RiboLock RNase inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... 1mM EGTA, 1mM EDTA, 1% Triton X-100, 0,27M sucrose, 1mM DTT, cOmplete EDTA-free protease inhibitor cocktail – Roche, 1mM NaF ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were incubated with 50 μl of 1:10 Enzyme:TUNEL reagent (TdT and fluorescein-dUTP) (Roche, Cat No. 11684795910) at 37°C for 3 h in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... or NETN Buffer (20 mM TRIS pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% IGEPAL, 1.25 mM DTT and Roche Protease Inhibitor) with several freeze/thaw cycles ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were rinsed twice with PBS and resuspended in 1 mL lysis buffer (1X PBS, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) supplemented with protease inhibitor cocktail (Roche, #11836170001). Chromatin was sheared to 200-500 base pairs using a Covaris E220 sonicator and cleared by centrifugation for 15 minutes at 19,000 × g ...
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bioRxiv - Biochemistry 2020Quote: ... cells were washed in ice-cold PBS and lysed in lysis buffer (100 mM TrisHCl pH 7.5, 150 mM NaCl, 1% Triton X-100, protease inhibitors (Roche)) for 30 minutes on ice ...
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bioRxiv - Cell Biology 2020Quote: ... before lysing in lysis buffer ((1% Triton X100, 50 mM HEPES, 150 mM KCl, 1 mM MgCl2, pH 7.4, with 1x EDTA-free protease inhibitors, Roche) at 4 °C for 2 hrs ...
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bioRxiv - Genetics 2021Quote: Neuronal cultures were lysed in 20 µl of NP40/SDS buffer (1% Nonidet P40/0.01% SDS lysis buffer and protease inhibitors (Roche) per well ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for one hour in donkey anti-mouse FITC antibody (1:200) in 0.3% Triton-X100 supplemented with 1X blocking buffer (Roche). After washing with PBS for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Pellets of cross-linked cells were resuspended in shearing buffer (10 mM Tris-HCl pH 8, 0.1% SDS, 1 mM EDTA) supplemented with Complete Protease Inhibitor Cocktail (Roche), and chromatin was sheared to an average fragment size of 200-250 bp ...
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bioRxiv - Genetics 2020Quote: ... Cells pellets were combined and lysed in a denaturing urea buffer (8 M urea, 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml ...
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bioRxiv - Cell Biology 2021Quote: ... A total of ∼ 8×107 cells were lysed in 2 mL lysis buffer (20 mM Tris HCl pH 7.4, 150 mM NaCl, 1% Triton-X 100 supplemented with protease inhibitor Complete, Roche). Lysates were rotated at 4°C for 20 minutes and subsequently centrifuged at maximum speed for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... coated with H-2Kb-OVA and ICAM-1 at a 1:1 ratio for various times at 37 ºC and immediately lysed in 2× cold lysis buffer containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4) and protease inhibitors (cOmplete mini cocktail, EDTA-free, Roche). Activation of PI3K and MAP kinase signaling was assessed by immunoblot for pAkt (Phospho-Akt (Ser473 ...
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bioRxiv - Immunology 2021Quote: Single-cells were sorted into PCR tubes containing 1μl of cell lysis solution (1:10 Cell Lysis buffer(Roche), 10U/μl Rnasin plus Ribonuclease inhibitor (Promega ...
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bioRxiv - Plant Biology 2020Quote: ... 150 mM NaCl, 5 mM EDTA, 5 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 1x protease inhibitor cocktail [Roche] ...
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bioRxiv - Neuroscience 2020Quote: ... Each tissue sample was dissociated using 2.4 mL of homogenization buffer (10 nM Tris pH 8, 5 mM MgCl2, 25 mM KCl, 250 mM sucrose, 1 μM DTT, 0.5x protease inhibitor [cOmplete, Roche #4693159001] ...
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bioRxiv - Molecular Biology 2020Quote: ... and subjected to 8 cycles of lysis in a MagNALyser (Roche – 6000 rpm, 1 min on, 2 min off). Lysate was transferred to fresh microfuge tubes and clarified by centrifugation (13,000 rpm ...
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bioRxiv - Cancer Biology 2021Quote: GSDMB2-HA expression was analyzed by immunohistochemistry in 5µm-thick tissue sections using rat anti-HA (1:200; 3F10, ROCHE) or mouse monoclonal anti-GSDMB (1:10 ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM ATP and 10% glycerol) containing 1 mmol/L PMSF and DNase I (Roche Diagnostics Corp., IN, USA) to 5 µg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... The medium was discarded and the cells were resuspended in 200 µL of ice-cold 50 mM HEPES buffer pH 7.4 containing 1 x protease inhibitor cocktail (Roche) and 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested 4 hours after induction at 37°C and lysed by sonication in 1x PBS pH 7.5 with 1 tablet protease inhibitor cocktail (Roche). After cell lysis and centrifugation at 30,000g ...
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C for 4 hr and washed two times with its resuspending buffer and once with E100 buffer (25 mM HEPES-KOH pH7.5, 10 % glycerol, 100 mM KCl, 2 mM MgCl2, 1 mM EDTA with Roche cOmplete™ ...
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bioRxiv - Neuroscience 2021Quote: ... 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor; Roche) and filtered through 40 μm filter (BD falcon ...
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bioRxiv - Neuroscience 2020Quote: ... The ipsi- and contralateral cortices were immediately transferred to lysis buffer (50 mM Tris, pH 7.6, 150 mM NaCl, 1% Triton X-100, CompleteMini Protease Inhibitor Mixture, Roche). The cortices were homogenized and incubated on ice for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with ice-cold 1x PBS added with 1.5 mM Na3VO4 and 50mM NaF before being lysed in lysis buffer (1X PBS, 1% Tx-100, 1.5 mM Na3VO4, 50mM NaF, PhosSTOP (Roche) and cOmplete protease inhibitors (Roche)) ...
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bioRxiv - Biochemistry 2021Quote: ... Mitochondria were resuspended in SEM buffer (250 mM sucrose, 1 mM EDTA, 10 mM MOPS-KOH, pH 7.2, containing 1X protease inhibitor cocktail from Roche).
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bioRxiv - Cell Biology 2021Quote: ... in lysis buffer (150 mM NaCl, 1% NP-40, 50mM Tris pH 8.0, 1x Roche protease inhibitor tablet (11697498001)) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with ice-cold 1x PBS added with 1.5 mM Na3VO4 and 50mM NaF before being lysed in lysis buffer (1X PBS, 1% Tx-100, 1.5 mM Na3VO4, 50mM NaF, PhosSTOP (Roche) and cOmplete protease inhibitors (Roche)) ...
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bioRxiv - Plant Biology 2020Quote: ... 0.5 mM PMSF) supplemented with 5 mg L−1 DNAse I and 0.5 g/L lysozyme (Roche, Mannheim, Germany). Cells were disrupted by sonication (3×2 min ...
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bioRxiv - Microbiology 2020Quote: Adherent macrophages in 6-well plates were washed with PBS containing 1 mM sodium orthovanadate and 10 mM 1,10-phenanthroline (Roche) on ice prior to lysis ...
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bioRxiv - Microbiology 2020Quote: ... pellets were re-suspended in 1.2 mL of Lysis Buffer (50 μg/ml lysozyme, 0.8% NP-40, 1 mM MgCl2, 1x protease inhibitors [Roche; cOmplete Protease Inhibitor Cocktail] in PBS) ...
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bioRxiv - Plant Biology 2020Quote: ... and homogenized in 100 μl ice-cold homogenization buffer (1% [v/v] SDS, 25 mM Tris/HCl, pH 7.5, 150 mM NaCl, 10 mM DTT and a Roche Complete protease inhibitor 1 tablet/10 ml) ...
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bioRxiv - Plant Biology 2021Quote: ... and 250 µl extraction buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 % Nonidet P40, proteinase inhibitor cocktail (Roche)) per 200 mg tissue powder was added ...
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bioRxiv - Genomics 2021Quote: ... 1 mL of lung dissociation medium (100 µg/mL of DNAse I, Roche; 1.25 mg/mL of Pronase, Roche; 9.2 µg/mL of Elastase ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were washed and re-suspended in nuclei lysis buffer (50mM Tris-HCl pH7.5, 10mM EDTA, 1% SDS, 1x Complete Proteinase Inhibitor (#11697498001, Roche)) ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed in ice cold 1% Triton lysis buffer (20mM Tris-HCl pH 8.0, 10mM EDTA, 1% Triton X-100 and 1x protease inhibitor cocktail (5892970001, Roche)) and protein concentration was measured using Bio-Rad BSA protein assay (5000116 ...