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Citations for Roche :
4851 - 4900 of 6945 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... and a biotin molecule (Supplementary Note 1) via PCR amplification with KAPA Hi Fi Polymerase (Roche) and nicking near the ends by the site-specific nicking enzyme Nt.BspQ1 (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were washed twice in PBST then incubated with DAPI (1 μg/ml in PBS; Roche) for 5 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... and washed with 1 mL 1X Wash Buffer (1 mL HEPES pH 7.5, 1.5 m 5M NaCl, 12.5 uL 2M spermidine and 47.5 mL dH2O with 1 Roche Complete Protease Inhibitor EDTA-Free tablet) ...
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bioRxiv - Neuroscience 2022Quote: ... or 1 mL CUT&RUN 1X wash buffer (1 mL HEPES pH 7.5, 1.5 m 5M NaCl, 12.5 uL 2M spermidine and 47.5 mL dH2O with 1 Roche Complete Protease Inhibitor EDTA-Free tablet) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) with the addition of two EDTA-free cOmplete protease inhibitor cocktail (Roche, 11836170001). Supernatant from lysed bacteria was collected by centrifugation at 16000 g for 10 minutes at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were incubated overnight at 4 °C with rat anti-HA Abs at 1:200 (Roche) and guinea-pig anti-red fluorescent protein (RFP ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue pellet was resuspended in a solution of HBSS with 1 mg/ml collagenase A (Roche) and 1 mg/ml DNase I (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: The following primary antibodies were used in this study: anti-HA (rat, 1:200, 11867423001, Roche), anti-V5 (mouse ...
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bioRxiv - Biochemistry 2022Quote: ... Cell membranes were solubilized in the lysis buffer plus 1% LMNG and Protease Inhibitor Cocktail (Roche) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 1% Triton X-100 and 10% glycerol in PBS) supplemented with a protease inhibitor cocktail (Roche) and 50 µM Palmostatin B (for SwissKASH-derived samples) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Hybridization signals were detected by Anti-Digoxigenin-FITC (Roche; dilution 1:10 in 0.5% BSA/PBS) and Streptavidin-Cy3 (Invitrogen Life Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated for 1h in 1% milk prepared in TBST with anti-GFP antibody (Roche). The blot was washed 3 times with TBST and incubated for 30 min in 1% milk prepared in TBST with anti-mouse HRP conjugate (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... ATP-induced dissociation was tested with an addition of running buffer containing ATP (1 mM, Roche) following binding of the MSH homolog.
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bioRxiv - Cancer Biology 2021Quote: ... 1%IGEPAL CA-630) supplemented with PhosSTOP phosphatase inhibitors and cOmplete protease inhibitor cocktail (both Roche). BCA protein assay (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: ... The cell lysates (1 μg/μL) were enzymatically digested with 50 μg/mL pronase E (Roche) at 37°C for 1 hour followed quenching with protease inhibitors and SDS-PAGE loading buffer ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of RIPA lysis buffer supplemented with complete protease inhibitor cocktail (cOmplete, EDTA-free [Roche] and phosphatase inhibitor cocktail (PhosSTOP [Roche] ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mM MgCl2) supplemented with 1% Triton-X100 and a mini complete protease inhibitor pill (Roche). Lysates were centrifuged at 20,000 × g for 15 min at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The culture medium was harvested and protease inhibitors (1 mM PMSF + 1X complete tabs from Roche) were added ...
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bioRxiv - Molecular Biology 2022Quote: ... Cat 15555799) and homogenized at 7000 g for 1 min using a MagNA Lyser (Roche diagnostic) at 4ºC ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1 mg/ml phenylmethyl sulfonyl fluoride (PMSF)) supplemented with 1% cOmpleteTM protease inhibitor cocktail (Roche Diagnostics). The tissue lysates were harvested and centrifuged at 12500×g for 15 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 1 mM EDTA) supplemented with protease inhibitor cocktail (cOmplete™, EDTA-free, Roche Cat. Nr. 11873580001) and phosphatase inhibitors for 30 min at 4 °C with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 mM TCEP) supplemented with Protease Inhibitor Cocktail (1 tablet/30 ml; complete, EDTA-free, Roche) and sonicated on ice ...
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bioRxiv - Developmental Biology 2022Quote: ... The samples were incubated with alkaline phosphatase (AP)-conjugated anti-DIG antibodies (1:2000, Roche, 11175041910) in 1% blocking buffer for 4 h at room temperature ...
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bioRxiv - Pathology 2019Quote: ... and 1 μg RNA was used for cDNA synthesis (Transcriptor first strand cDNA synthesis kit, Roche). INF2 was then PCR amplified from cDNA using isoform-specific primers (Supplementary Figure 1 ...
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bioRxiv - Physiology 2020Quote: ... 1%v/v Triton X-100) with protease inhibitors (cOmplete Tablets EDTA-free, EASYpack, Roche, 04693132001). Resulting nuclei were centrifuged at 12,000 RPM and resuspended in shearing buffer (1% SDS ...
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bioRxiv - Molecular Biology 2019Quote: ... DIG-labeled probes were detected using mouse monoclonal anti-DIG primary antibody (Roche; 1:100 dilution) and a Cy3-labeled goat anti-mouse IgG secondary antibody (Roche ...
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bioRxiv - Systems Biology 2019Quote: ... and then washed three times with 1 ml Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, 1 × Roche cOmplete EDTA-free mini protease inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... PfAP2-G and PfAP2-I were detected with 1 μg/mL rat α HA (Roche 3F10) and 2 μg/mL rabbit α GFP (Abcam ab290) ...
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bioRxiv - Cancer Biology 2021Quote: ... Single cell suspensions were then prepared by incubating minced tissue in 1 mg/ml collagenase (Roche) and 50 U/ml DNase I (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to a new tube and combined with 1 μl glycogen (Roche, 10901393001), 40 μl 3M sodium acetate ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA] supplemented with cOmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails (Roche). Clarified cell lysates were incubated with Streptactin Sepharose beads (IBA ...
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bioRxiv - Neuroscience 2019Quote: ... TREM2 ICDs were detected using an anti-HA HRP-coupled antibody (clone 3F10, Roche, 1:250). As a positive control ...
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bioRxiv - Neuroscience 2019Quote: ... Probes were detected using a 1h incubation at RT with anti-fluorescein-POD (1:2500, Roche) and anti-DIG-POD antibodies (1:2500 ...
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bioRxiv - Plant Biology 2020Quote: ... Primary and secondary antibody dilutions were as follows: α-GFP 1:1500 (Roche Diagnostics; Basel, Switzerland), α-RFP 1:1000 (ChromoTek ...
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bioRxiv - Microbiology 2019Quote: ... Contaminating DNA was digested by DNase I (Roche; 1 U/µg RNA, 60 min, 37°C) in the presence of RNase inhibitor (RNaseOUT ...
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bioRxiv - Developmental Biology 2019Quote: ... Probes were detected by over-night incubation with anti-Digoxigenin-AP Fab fragments (1:5000) (Roche) and stained with standard Nitro Blue Tetrazolium (NBT ...
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bioRxiv - Genomics 2021Quote: ... Second-stage digestion of SUMOylated peptides was performed with 1 μg of Endoproteinase Asp-N (Roche). Digestion was performed overnight ...
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bioRxiv - Plant Biology 2021Quote: ... 6xHis-HA-RGL2-6His and its ubiquitinated conjugates were detected using anti-HA (1:1000; Roche) antibody.
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bioRxiv - Cell Biology 2020Quote: ... 1% Triton X-100 and 2 mM phenylmethylsulfonyl fluoride and Complete Protease Inhibitor cocktail (Roche Diagnostics). 2 µg of the extracted proteins were separated by SDS-PAGE gel and stained with CBB R-250 ...
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bioRxiv - Biochemistry 2021Quote: ... 25 mM KCl containing 1% (w/v) digitonin and 1X Protease Inhibitor mix (Roche, EDTA-free). After centrifugation (15 min ...
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bioRxiv - Biochemistry 2021Quote: ... 25 mM KCl containing 1% (w/v) digitonin and 1X Protease Inhibitor mix (Roche, EDTA-free), or by sonication on ice (3x 10s ...
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bioRxiv - Cell Biology 2021Quote: ... On Day 0 (start of differentiation) hPSCs were treated with 1 mg/ml Collagenase B (Roche) for one hour ...
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bioRxiv - Immunology 2021Quote: ... washed and rested for 1 h in 0.25 µL/mL DNase I (Roche Diagnostics, Indianapolis, IN)-containing R-10 complete medium (RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... and digested in 2.5 ml of culture media containing 1 mg/mL of Collagenase IV (Roche) and 50 unit/mL DNase (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... then incubated in the same buffer for 3h with anti-DIG-POD antibodies (1:500, Roche) and revealed using the kit TSA-Plus Cyanin-3 (Perkin Elmer).
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bioRxiv - Immunology 2020Quote: ... The bacterial pellet was resuspended in 1 ml of PBS containing protease inhibitors (cOmplete Mini, Roche; 1 tablet per 10 ml of PBS following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Digoxigenin FISH signals were amplified using sheep anti-digoxigenin fluorescein fab fragments (1:50, Roche 11207741910) and fluorescein rabbit anti-sheep antibodies (1:100 ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.3% 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS)) supplemented with 1X protease inhibitor cocktail (Complete; Roche), 0.5 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Immunology 2020Quote: ... 10% glycerol and 1% Triton X-100) supplemented with a protease and phosphatase inhibitor mixture (Roche) for 20min on ice ...
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bioRxiv - Genetics 2019Quote: ... Cells were scraped in PBS supplemented with protease inhibitor cocktail (1:100, 11836145001, Roche applied science) and phosphatase inhibitor cocktail (1:100 ...