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Citations for Roche :
5001 - 5050 of 6945 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cell proliferation was quantified after 48 h of incubation using cell proliferation reagent WST-1 (Roche, 11644807001) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell pellet was treated with 1 ml of Red Blood Cell Lysis Buffer (Roche, Solna, Sweden) for five minutes at room temperature ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... 1 mM EDTA) freshly supplemented with protease inhibitors (cOmplete™, Mini Protease Inhibitor Cocktail, Roche-Merck, #11836153001) and lysed for 20 minutes on a rotating wheel at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... The embryos were subsequently incubated in the blocking solution (2%Roche block, 5% calf serum, 1% DMSO). γH2AX antibody (GTX127342 ...
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bioRxiv - Cell Biology 2019Quote: ... 1% Triton X-100 plus protease and phosphatase inhibitor cocktails (Complete Mini, EDTA-free; PhosSTOP; Roche Diagnostics). Insoluble material was cleared by centrifugation for 10 min at 20,000xg ...
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bioRxiv - Microbiology 2019Quote: ... Cells were subsequently solubilised in 1% Triton X-100 in PBS containing 1x cOmplete protease inhibitor (Roche) and incubated on ice for 20 min ...
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bioRxiv - Microbiology 2019Quote: ... Infected red blood cells were lysed on ice with chilled hypotonic buffer (1 mM HEPES/NaOH, Roche cOmplete EDTA-free protease inhibitor ...
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bioRxiv - Microbiology 2019Quote: ... pH 8.0) containing 1 mg/mL lysozyme and one complete mini EDTA-free protease inhibitor pellet (Roche). Cell suspensions were incubated on ice for 20 min before sonication ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was probed with a 1:20000 dilution of mouse anti-GFP antibody (JL-8, Roche) or mouse anti-alpha-tubulin antibody (B-511 ...
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bioRxiv - Biophysics 2019Quote: ... 1% (v/v) Triton X-100 and protease inhibitors (cOmplete mini EDTA-free protease inhibitor cocktail; Roche) were added to one well and the plate incubated overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: The following primary antibodies were used in the specified concentrations: mouse anti-GFP (1:1000, Roche #11814460001), rat anti-HA (1:1000 ...
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bioRxiv - Immunology 2021Quote: Sorted cells or stimulated cells were lysed in RIPA buffer (50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM PMSF, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1x Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Following hybridization, the sections were treated with secondary antibody anti-dig-ap (1:1000, 11093274910, Merk (Roche), UK) ...
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bioRxiv - Developmental Biology 2020Quote: ... Tissue sections were incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:1500, Roche Applied Sciences 11093274910) for 16-20h incubation at room temperature and colorimetric signals were detected using Nitro blue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl phosphase (NBT-BCIP ...
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bioRxiv - Developmental Biology 2021Quote: ... DIG was detected with anti-DIG Fab fragments conjugated to alkaline phosphatase (Roche, Indianapolis IN; 1:2000), and the chromogenic reaction performed using BM purple (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was evaluated using a colometric formazan-based cell viability assay (WST-1; Roche, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... 1% NP 40 and 0.5M EDTA with 1x protease Mini-complete protease inhibitor (04693124001; Roche, Indianapolis, IN) and 1x phosphatase inhibitor cocktail (4906845001 ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblot with the anti-His-antibody conjugated to peroxidase (BMG-His-1 monoclonal antibody; Roche, Basel, Switzerland) was used to confirm the presence of enzymes.
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bioRxiv - Plant Biology 2021Quote: ... 2 M NaCl 1 mM TCEP) supplemented by one protease inhibitor cocktail tablet Complete EDTA-free (Roche) and centrifuged for 30 min at 18,000 g 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.4 supplemented with 1 µg/mL Dnase I and cOmplete EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mM β-mercaptoethanol and supplemented with complete EDTA-free cocktail tablets (1 tablet/50ml cells; Roche), 0.01 mg/ml DNase (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... The protein was extracted by adding ice-cold 10 v/w RIPA buffer (250 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris HCl, pH 8.0, containing Roche cOmplete™ protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail and 1mM AEBSF ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% Triton X-100 supplemented with protease and phosphatase inhibitor cocktail tablets (Roche Diagnostics, Mannheim, Germany). Liquid N2 was used to devastate cell membranes to collect whole protein lysate ...
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bioRxiv - Plant Biology 2021Quote: ... 2mM EDTA) containing 1% Triton-X100 and protease inhibitors (cOmplete EDTA-free®, Roche catalogue no 04693132001). For immunoprecipitation ...
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bioRxiv - Plant Biology 2020Quote: ... resuspended in 25 ml ice-cold lysis buffer (20 mM Hepes-KOH pH 8.0, 0.5 M NaCl, 1 mM EDTA, 0.1% Triton X-100, Roche cOmplete™ EDTA-free protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2020Quote: ... washed for 30 min with washing buffer (1 × maleic acid buffer + 0.3% Tween 20; Roche, Catalog# 11585762001), then equilibrated for 3 min in detection buffer (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... 1% Triton X-100) supplemented with PMSF and the cOmplete(tm) protease inhibitor cocktail (EDTA-free; Roche). Lysates were subjected to SDS-PAGE and transferred to PVDF membrane (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies against the following antigens were used at the indicated dilutions: GFP (Mouse, 11814460001, Roche, 1/5000), HA (Rabbit ...
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bioRxiv - Cell Biology 2021Quote: ... 1% (v/v) Triton X-100) containing 150 mM NaCl and EDTA-free protease inhibitor Complete (Roche) for 20 to 30 min at 4°C (Kessels & Qualmann ...
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bioRxiv - Cell Biology 2022Quote: ... followed by lysis with 1% (w/v) SDS in PBS-containing protease inhibitors mixture (Roche Applied Science) and phosphatases inhibitors mixture (1 mM sodium pyrophosphate ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight with a peroxidase conjugated anti-DIG antibody (1:200, Roche, Mannheim, Germany) and primary antibodies for GFP/YFP (chicken anti-GFP ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were transfected with a total of 1 µg of DNA using Fugene 6 (Roche, Branchburg, NJ) according to the manufacturer’s instructions
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with alkaline phosphatase-conjugated anti-DIG antibody (1:1000, Roche Applied Science, cat# 1093274) and chicken anti-GFP antibodies (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... 1% Triton-X-100 supplemented with 1X protease inhibitors (CompleteTM Mini, EDTA-free Protease Inhibitor Cocktail, Roche) and 1/100 diluted phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2 ...
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bioRxiv - Genomics 2022Quote: ... and 12.5 μL of second-strand synthesis mixture [1× KAPA Hifi ReadyMix (KAPA Biosystems, Wilmington, MA, USA) and 0.4 µM 5’universal-9G primer] was added ...
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bioRxiv - Neuroscience 2022Quote: ... and 1 mM DTT) containing 200 µg/mL lysozyme and an EDTA-free protease inhibitor cocktail (Roche Diagnostics ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were probed overnight at 4 °C with anti-GFP primary antibodies (Roche 11814460001; 1:1000) and then with anti-mouse peroxidase-conjugated secondary antibodies (Jackson Immunoresearch # 115-035-146 ...
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bioRxiv - Pathology 2022Quote: ... supplemented with 1 tablet of Protease inhibitors (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail, Roche #11836170001) per 10ml lysis buffer ...
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bioRxiv - Immunology 2022Quote: OMVs were lysed with 1% Triton supplemented with cOmplete™ Protease Inhibitor Cocktail Tablets (cat# 11697498001, Roche). Samples were mixed with sample buffer with/without dithiothreitol (DTT) ...
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bioRxiv - Developmental Biology 2022Quote: ... E15.5 fetal stage thymi were dissected and digested in 1 mg/ml collagenase/dispase (Roche, Basel, Switzerland) and passed through a 100 μm mesh to remove debris ...
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bioRxiv - Cancer Biology 2022Quote: ... DF-1 cells were transfected with the relevant RCAS viral plasmids using Extreme-Gene 9Transfection reagent (Roche) accordingly to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Tubes were placed on a magnet stand to remove unbound antibody and washed three times with 1 ml digitonin buffer (20 mM HEPES pH 7.5, 0.15 M NaCl, 0.5 mM Spermidine, 1x Roche complete protease inhibitors ...
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bioRxiv - Bioengineering 2022Quote: ... PMSF (MP Biomedical) and protease inhibitor tablets (1 tablet/100 mL, cOmplete Mini EDTA-Free, Roche Diagnostics), 5ml / g wet weight of pellet ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked by incubation in 5% (w/v) milk power or 1 × Western Blocking Reagent (Roche) in Tris-buffered saline and 0.1% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... 25 mM potassium chloride and 1 mM EDTA) in the presence of a protease inhibitor cocktail (Roche). Cell lysis was performed using mechanical homogenisation with constant incubation of the samples on ice ...
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bioRxiv - Cell Biology 2022Quote: ... the following antibodies were used (all at 1:3000 dilution): α-GFP (Roche, 11 814 460 001), α-Pgk1 (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were resuspended in 200 µL PBS containing 1× Complete EDTA-free protease inhibitor cocktail (Roche) and sonicated by Bioruptor II (BMBio) ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were washed twice with 1 ml PA-150 buffer containing EDTA-free protease inhibitor cocktail (Roche) (500 × g ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... 1 mM EDTA) freshly supplemented with protease inhibitors (cOmplete™, Mini Protease Inhibitor Cocktail, Roche-Merck, #11836153001), followed by brief sonication ...
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bioRxiv - Neuroscience 2021Quote: ... The following antibody cocktails were used: rat anti-HA (1:200, Cat. No. 10145700, Roche Diagnostics, USA) with rabbit anti-CamKIIα (1:200 ...