Labshake search
Citations for Roche :
4751 - 4800 of 5576 citations for Mouse Legumain ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Illumina sequencing library was prepared using a TruSeq-based protocol (KAPA LTP Library Preparation kit Illumina, catalog num. 7961898001, Roche) and manufacturer protocol was strictly followed ...
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bioRxiv - Microbiology 2023Quote: ... and nucleic acid extracted using the MagNA 96 instrument with DNA/Viral NA small volume kits (Roche, Laval, Quebec, Canada). Ophidiomyces ophidiicola was not detected in these samples by qPCR (Allender et al ...
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bioRxiv - Evolutionary Biology 2023Quote: ... present-day mitochondrial genome was amplified for baits in four overlapping fragments using the Expand Long Range dNTPack kit (Roche) with four selected primers pairs (175F-5151R ...
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bioRxiv - Genomics 2023Quote: ... All strains were sequenced using an Illumina MiSeq platform and the KAPA LTP library preparation kit (KAPA Biosystems, Wilmington, MA). The pooled libraries were loaded into a MiSeq system and sequenced using a MiSeq reagent kit v2 with 2 × 250 cycles (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using a FastStart Universal Probe Master (Rox) kit (Roche Applied Science) or Luna Universal Probe qPCR Master Mix (New England Biolabs ...
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bioRxiv - Pathology 2023Quote: Total RNA was extracted from sigmoid colon tissue using a High Pure RNA Isolation kit (Roche Applied Science, Penzberg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... ChIP-seq libraries were prepared by Duke University Sequencing and Genomic Technologies Core Facility (Duke Sequencing Facility) from 1 ng of ChIP DNA using the KAPA HyperPrep Library Kit (Roche) to generate Illumina-compatible libraries ...
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bioRxiv - Neuroscience 2023Quote: Apoptotic cell death was assessed as formation of fragmented nucleosomes using the Cell Death Detection ELISAPlus kit (Roche Applied Science) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Afterwards 5ng DNA fragments were pooled and proceeded on with library construction using KAPA Hyper Prep Kit (Kapa Biosystems #KK8504) according to the standard protocol ...
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bioRxiv - Microbiology 2023Quote: ... qPCR analysis was done on 100ng of DNA using a LightCycler 480 Probe Master-Mix kit (Roche, Cat. No. 04707494001) and a primer and probe set specific to MHV68 gB to quantify the number of viral genome copies ...
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bioRxiv - Molecular Biology 2023Quote: 100 pmol gapmers (Supplemental Table S4) were labeled with biotin-16-ddUTP (Jena) and a 2nd generation DIG-oligonucleotide 3’end-labeling kit (Roche) using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer ...
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bioRxiv - Immunology 2023Quote: ... lactate dehydrogenase (LDH) was measured in supernatants collected at 48 hours post transfection using cytotoxicity detection kit PLUS Assay (CYTODET-RO, Roche) according to manufacturer’s instructions and plates were read at 490 nm.
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bioRxiv - Microbiology 2023Quote: ... Libraries were constructed using ∼50 ng of mRNA using the Kapa Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems, Illumina). Indexes were designed according to TruSeq RNA UD specifications (#20020591 ...
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bioRxiv - Biochemistry 2023Quote: Cytotoxicity assays were performed as described29 (with minor changes. Proliferation of human cells was assessed using an MTT colorimetric assay (Cell Proliferation Kit I, Roche). HeLa (epithelial cells ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNAs were labeled with [α32P]-dCTP using the Random Primed DNA Labeling Kit following the protocol provided by Roche. Total RNA was TRizol (Invitrogen ...
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Remodeling of the Ribosomal Quality Control and Protein Translation by a Viral Ubiquitin DeconjugasebioRxiv - Cell Biology 2023Quote: ... One μg total RNA was reverse transcribed using the SuperScript VILO cDNA Synthesis kit and quantified using a LightCycler 1.2 instrument (Roche Diagnostic) with the LC FastStart DNA master SYBR green I kit and specific primers (Supplementary Table S3) ...
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bioRxiv - Developmental Biology 2023Quote: Apoptotic cells present in gonadal histological preparations were revealed by the terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labelling (TUNEL) assay using the Fluorescent In Situ Cell Death Detection Kit (Roche ref ...
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bioRxiv - Genomics 2022Quote: ... RNA-seq libraries were prepared using 1 μg of total RNA input using KAPA stranded mRNA-seq kit for Illumina (Roche) as per manufacturer’s instruction and paired-end sequenced on NovaSeq 6000 (Illumina).
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining on 60 dpf paraffin embedded testes sections were performed using a fluorescein In Situ Cell Death Detection Kit (11684795910, Roche). Immunolabelling with Vasa antibody was done before the TUNEL staining ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxigenin (DIG)-labeled sense and antisense riboprobes were prepared using the in vitro transcription DIG labeling kit (Roche, IN, USA). Hybridization was carried out at 600C overnight and the slides were washed and probed with an alkaline phosphatase-conjugated anti-DIG antibody (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was done following manufacturer’ s instruction (In Situ cell Death Detection Kit TMR red, Roche).
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bioRxiv - Genomics 2023Quote: ... 1ng of Cut and Run DNA was used to make libraries using the KAPA Hyper prep kit and SeqCap adapters A &B (Roche) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The 12 and 32 libraries were equimolarly pooled and subjected to qPCR quantification using the KAPA library quantification kit (Roche). Sequencing was carried out on a NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... rRNA-depleted RNA-seq libraries were then amplified in a BioRad CFX96 using the Kapa HiFi Real-Time Amplification Kit (Roche) in a 50 μL reaction with 25 μL master mix ...
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bioRxiv - Developmental Biology 2023Quote: RNA sequencing (RNAseq) libraries were prepared using 1 µg of total RNA using the KAPA stranded mRNA-seq kit (Roche) according to the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2022Quote: ... or IRF4-R98AC99A variants was processed by use of the KAPA mRNA Hyper Prep Kit for Illumina Platforms (KK8580; Roche) and KAPA Single-Indexed Adapter Kit (KK8700 ...
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bioRxiv - Systems Biology 2023Quote: ... Paired-end RNA sequencing libraries were prepared by using a KAPA RNA HyperPrep kit from Kapa Biosystems (Wilmington, MA, USA) as described previously33 ...
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bioRxiv - Immunology 2023Quote: ... Lactate dehydrogenase (LDH) in the supernatants from either cell type was quantified using the LDH Cytotoxicity Detection kit (TaKaRa or Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cells were plated in 96-well plates and treated as indicated for 72 hours before performing MTT survival assay using Cell Proliferation Kit 1 MTT (Roche) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: RNA was extracted at 120 h or 144 h post aggregation using the High Pure RNA Isolation Kit (Roche 11828665001) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The library was prepared by the Dana Farber Next Generation Sequencing Core Facility using a KAPA library quantification kit (Roche). Sequencing was done on an Illumina NovaSeq 6000 with total RNA.
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bioRxiv - Immunology 2023Quote: ... cytotoxicity was determined based on the release of lactate dehydrogenase (LDH) into the co-culture supernatant measured by Cytotoxicity Detection Kit (Roche) and using a Synergy 2 Multi-Mode Microplate Reader (Biotek ...
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bioRxiv - Immunology 2023Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Developmental Biology 2023Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed using the In Situ Cell Death Detection Kit (11684795910, Roche) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... A dioxigenin (DIG)-11-UTP-labeled antisense riboprobe targeting the gene transcript of interest was synthesized using the DIG labelling kit (Roche) and T7 RNA polymerase80 ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time PCR was performed in triplets for each sample using the LightCycler DNA Master SYBR Green I Kit in a LightCycler 480 System (Roche). The relative copy number of Gapdh RNA was used for normalization ...
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bioRxiv - Microbiology 2023Quote: ... five microliter of elution was loaded into a 96-well plate with the Light Cycler RNA master hybridization Probe kit (Roche) and amplification was performed in a Lightcycler 480 machine (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... or Ultra View Universal Alkaline Phosphatase Red Detection Kit (yielding pink signals) using standard machine settings (all solutions were from Ventana, Roche). Counterstaining (light blue background ...
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bioRxiv - Systems Biology 2023Quote: ... the entire eluate was used as input for a 300 μl PCR reaction mastermix with the KAPA HiFi HotStart DNA Polymerase kit (Roche Sequencing Solutions Inc ...
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bioRxiv - Cancer Biology 2023Quote: ... The Van Andel Genomics Core prepared RNA libraries from 500ng of total RNA using a KAPA mRNA Hyperprep kit (v4.17, Kapa Biosystems). RNA was sheared to 300-400bp ...
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bioRxiv - Neuroscience 2023Quote: ... RNA-seq libraries were prepared by the Van Andel Genomics Core from total RNA using the KAPA RNA HyperPrep Kit with RiboseErase (v1.16) (Kapa Biosystems). Base calling was done by Illumina NextSeq Control Software (NCS ...
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bioRxiv - Molecular Biology 2023Quote: ... the cDNA was used for real-time quantitative PCR (qPCR) with the Kapa HiFi HotStart Library Amp kit (Roche, KK2612). For Illumina short-read sequencing ...
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bioRxiv - Neuroscience 2023Quote: The TUNEL assay was caried out using the In Situ Cell Death Detection TMR rot kit (Roche, cat. no. 12156792910) according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2023Quote: ... RNA sequencing libraries were prepared with 200 ng of total RNA using KAPA RiboErase Stranded RNA-Seq kit (cat # KK8483) following standard protocol (Roche). Sequencing was done at Stanford Functional Genomics Facility (Stanford ...
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bioRxiv - Genomics 2023Quote: ... Library preparation was performed using the Kapa Hyper Prep library preparation reagents following the manufacturer’s protocol (Kapa Hyper Prep Kit, Roche 07962363001). ChIP libraries were sequenced using the Illumina HiSeq 4000 at 50 bp single reads or NovaSeq 6000 at 100 bp single reads.
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bioRxiv - Microbiology 2023Quote: ... Probes were obtained by PCR using the primers pGK-S55 and pGK-S53 (Table S1) and labelled with the PCR DIG Probe Synthesis Kit (Roche) according to the manufacturer and revealed using the DIG Luminescent Detection Kit and DIG Easy Hyb (Roche).
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bioRxiv - Genomics 2023Quote: High quality short-read libraries were also generated and sequenced from the same DNA samples using a Kapa HyperPrep kit (Roche) and a DNBseq G50 platform by BGI Genomics (Hong Kong ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified libraries were controlled by capillary electrophoresis (LabChip - Perkin) and quantitated by qPCR (KAPA Library Quantification Kits Illumina Platforms - Roche). Pooled libraries (12 pM ...
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bioRxiv - Molecular Biology 2023Quote: cDNA libraries were generated from the RNA samples using the Kapa RNA HyperPrep Kit with RiboErase (Roche, Indianapolis, IN, USA). Quality was measured by Agilent D1000 Screen Tape (Santa Clara ...
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bioRxiv - Cancer Biology 2023Quote: ... or OSU-ERb-12 (100nM) + Enza (1uM) for 48h and processed as previously(24) reported using Annexin-V-FLUOS staining kit (Roche) and Propidium Iodide ...