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Citations for Roche :
4601 - 4650 of 5576 citations for Mouse Legumain ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... TUNEL staining was conducted following manufacturer’s protocol (In Situ Cell Death Detection Kit, Version 17, Roche Diagnistics GmbH, Mannheim, Germany). Briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µg of total RNA was converted to a sequence library using a KAPA Stranded mRNA-seq Kit (KAPA Biosystems). These libraries were analyzed using a HiSeq 2500 sequencer (Illumina ...
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bioRxiv - Developmental Biology 2019Quote: ... data in the KS cohort was performed on peripheral blood-derived DNA using either Nimblegen SeqCap target enrichment kit (Roche) or a custom Illumina capture kit (ICE ...
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bioRxiv - Cancer Biology 2019Quote: ... Nucleosomal DNA was isolated and either used as a template for qPCR (ChIP-qPCR) or to generate NGS libraries using KAPA Hyper Prep Kits (Roche) and TruSeq adaptors (Bioo Scientific ...
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bioRxiv - Genomics 2021Quote: ... 10.6 kbp and 16.4 kbp runs were constructed using the Oxford Nanopore Technologies (ONT) SQK-LSK109 kit according to the manufacturer’s instructions except that Kapa beads (Roche, UK) were used to perform the clean up steps rather than Ampure XP beads ...
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bioRxiv - Genetics 2020Quote: ... One short-insert WGS library was constructed using the Kapa Hyper Prep Kit (KR0961) from Kapa Biosystems (Woburn, MA, USA) with 150 ng of sheared DNA as input following standard manufacturer’s protocol with 10 cycles of PCR ...
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bioRxiv - Physiology 2021Quote: ... All mice were genotyped by polymerase chain reaction (PCR) using KAPA Taq PCR Kits (Kapa Biosystems, Inc., Wilmington, MA, USA). Specific primers purchased from Integrated DNA Technologies (Coralville ...
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bioRxiv - Physiology 2020Quote: 1 μg RNA was reverse transcribed into cDNA with Oligo(d)T primers using the Transcriptor First Strand cDNA Synthesis kit (Roche). The qRT-PCR was performed with the LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Physiology 2021Quote: ... cDNA libraries were validated using high sensitivity DNA chip on the Agilent 2100 Bioanalyzer and quantified using the KAPA Library Quantification Kit (Roche). Indexed libraries were pooled and sequenced with a 50-bp single-end protocol on a HiSeq4000 platform (Illumina ...
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bioRxiv - Physiology 2021Quote: ... Plasma total triglycerides (Total TG) and total cholesterol (Total Chol) were measured by enzymatic assay using commercially available kits (Roche Diagnostics GmbH ...
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bioRxiv - Molecular Biology 2020Quote: ... Only one sample was obtained per patient: one portion of the sample was extracted using the High Pure Viral RNA Kit (Roche), and another portion of the same sample was extracted using the acid pH method ...
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bioRxiv - Microbiology 2020Quote: ... Total RNAs were extracted from 200 μl of mice sera using a High Pure viral RNA kit (Roche Applied Science) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were generated by random shearing of genomic DNA followed by ligation of custom adapters using the Kapa HyperPrep Kit (Roche). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... The resulting RNA was used as a matrix for reverse transcription using the AMV cDNA first strand synthesis kit (Roche). The produced cDNA was used to assess ecpD expression as well as 16S rRNA gene as a control.
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bioRxiv - Genomics 2020Quote: ... These DNA fragments were labeled with digoxigenin (DIG) using a DIG High Prime DNA Labeling and Detection Starter Kit II (Roche), and signals were detected according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Metabolic activity was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-assay (Cell Proliferation Kit I, Roche Germany, Mannheim) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: Pitx1 WISH were performed on 40-45 somite stage mouse embryos (E12.5) using a digoxigenin-labeled Pitx1 antisense riboprobe transcribed from a cloned Pitx1 probe (PCR DIG Probe Synthesis Kit, Roche), as previously described in (Kragesteen et al ...
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bioRxiv - Plant Biology 2021Quote: ... Antisense or sense strand digoxygenin-UTP labeled RNA was generated for PEPCK and NADP-ME using a DIG RNA labeling kit (Roche). For PEPCK hybridizations ...
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bioRxiv - Immunology 2021Quote: ... The qPCR reactions were performed in triplicate for each cDNA sample using the Thunderbird Next SYBR qPCR Mix kit (Toyobo) on Light Cycler 96 (Roche). The primers used were the followings ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was extracted for tissues with sufficient tissue and bulk RNAseq libraries were prepared with KAPA mRNA HyperPrep kit (Roche). Bulk RNA sequencing data was processed with the same pipeline as the GLASS samples.
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bioRxiv - Neuroscience 2021Quote: Digoxigenin RNA-labeled or Fluorescein RNA-labeled probes were transcribed in vitro using the RNA Labeling Kit (Roche Diagnostics Corporation) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
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bioRxiv - Cancer Biology 2020Quote: ... The short-insert paired-end libraries for the whole genome sequencing were prepared with KAPA HyperPrep kit (Roche Kapa Biosystems) with some modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... The short-insert paired-end libraries for the whole genome sequencing were prepared with KAPA HyperPrep kit (Roche Kapa Biosystems) with some modifications ...
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bioRxiv - Genomics 2019Quote: ... from each specimen underwent cDNA synthesis and construction of sequencing libraries using a KAPA Stranded RNA-Seq Library Preparation Kit (Roche Sequencing ...
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bioRxiv - Genetics 2021Quote: We extracted DNA from nail samples using the ISOHAIR (Nippon Gene) and oral mucosa samples using the High Pure PCR Template Preparation Kit (Roche).
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bioRxiv - Genomics 2020Quote: Barcoded 2×300 bp metagenomic libraries were prepared with the KAPA Hyperplus Library Preparation kit (KAPA Biosystems, Wilmington, MA, US) and sequenced on an Illumina MiSeq system at the National Oceanography Centre (Southampton ...
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bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
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bioRxiv - Cancer Biology 2021Quote: Reverse transcription for qPCR analysis was done with the first strand synthesis kit (Fermentas) using oligo (dT)18 as a primer and qPCR was carried out with LightCycler 96 (Roche) using FastStart Essential DNA Green Master Mix (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: The in vitro synthesis of digoxigenin (DIG)-labeled antisense SatIII probe (158 bp SatIII repeat cloned in 1μg of PGEM-2-98 construct) with T7 RNA polymerase was carried out using the DIG labeling kit from Roche Products Pvt ...
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bioRxiv - Cancer Biology 2021Quote: ... libraries were prepared in an automated fashion using an Agilent Bravo robot with aKAPA Standard mRNA-Seq Kit (KAPA BIOSYSTEMS). In-house sequencing adaptors were ligated to 100-300 bp fragments of input dsDNA ...
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bioRxiv - Genomics 2019Quote: ... Genome libraries were prepared using the KAPA Hyper Library Preparation Kit with Standard PCR Library Amplification (Kapa Biosystems, Wilmington, MA) and sequenced on an Illumina MiSeq using V3 sequencing chemistry (Illumina Inc. ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed using the LightCycler 480 Probe Master Kit and the LightCycler 480 II system (Roche Applied Science) as described previously92 ...
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bioRxiv - Microbiology 2021Quote: ... Radiolabeled probe was generated by purifying a digested (Ncol; 1 cut) pCpG vector DNA using High Pure PCR product Purification Kit (Roche) followed by radiolabeling the probe using Random Prime DNA labeling kit (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... Illumina compatible Indexed libraries were prepared from 12ng of Diagenode Biorupter Pico sheared ChIP DNA using the KAPA Hyper Library Preparation Kit (Roche). Libraries were enriched with 2 cycles of PCR then assessed for size distribution using the 4200 TapeStation High Sensitivity D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... 1μl of cDNA diluted with 10-fold MQ was mixed with KAPA SYBR Fast qPCR kit and amplified with Lightcycler 96 (Roche; 95℃ 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Triplicate reactions that amplified were pooled together and quantified with Kapa library quantification kit (Kapa Biosystems, KK4824, Wilmington, MA, USA) before equimolar sample mixing ...
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bioRxiv - Immunology 2020Quote: ... TUNEL assays were performed on sectioned tissue using the TMR red In Situ Cell Death Detection Kit (Roche, Basel, Switzerland), following the manufacturer protocol for labeling of cryopreserved tissues ...
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bioRxiv - Immunology 2020Quote: ... The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Equimolar amounts of each library were sequenced on a NextSeq 500 instrument controlled by the NextSeq Control Software (NCS ...
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bioRxiv - Immunology 2020Quote: ... Amplifications were carried out on a LightCycler 480 II instrument using LightCycler 480 software (v. 1.5.0) and a LightCycler 480 SYBR green I master kit (Roche Applied Science) according to the provided instructions ...
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bioRxiv - Immunology 2021Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Immunology 2021Quote: ... cell nuclei with DNA strand breaks at the early stage of apoptosis were labeled using fluorescein-dUTP in the TUNEL reaction (In Situ Cell Death Detection Kit; Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... finally hybridized for 3h at 42 °C with DIG-labelled telomere probes and bulk telomere length analysed as recommended by the manufacturer (Telo-TAGGG Telomere Length Kit Assay; Roche). For TRF on APC vesicle preparations ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from homogenized tissue of adenovirus vectored vaccine inoculated mice by High Pure Viral Nucleic Acid Kit (Roche). DNA fragments of Sad23L and Ad49L vectors were amplified by nested PCR with primers specific to adenoviral hexon sequences (Table S4 ...
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bioRxiv - Immunology 2021Quote: ... Poly A mRNA was purified from total RNA using oligodT magnetic beads and strand-specific indexed libraries were prepared using the Illumina’s TruSeq Stranded mRNA Sample Prep Kit followed by ten cycles of amplification using KAPA HiFi DNA polymerase (KAPA Biosystems). Libraries were quantified and pooled based on a post-PCR Bioanalyzer and 75 base pair (bp ...
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bioRxiv - Immunology 2021Quote: ... Synthesized DNA specific to regions of homologous recombination (see Oligonuleotide section in Key Resources Table) was labeled with 32P (Amersham) using the High Prime labeling kit (Roche) according to manufacturer’s instructions and unincorporated 32P was removed with G-50 Sephadex Quick Spin columns (Fisher) ...
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bioRxiv - Immunology 2020Quote: ... Telomere dot blot was performed by 3h hybridization at 42°C with DIG-labelled telomere probe as recommended by the manufacturer (Telo-TAGGG Telomere Length Kit Assay; Roche). Control Input was 200 ng genomic DNA from APCs ...
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bioRxiv - Immunology 2021Quote: Sample RNA extraction was performed from freshly isolated C57BL/6J WT pMBEMCs following the manufacturers instruction of the High Pure RNA Isolation Kit (Hoffman-La Roche). cDNA was obtained from total isolated RNA of each sample with the SuperScript III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Genetics 2019Quote: ... RNA mRNA libraries of template molecules suitable for high throughput DNA sequencing were created using the KAPA mRNA HyperPrep Kit (Roche). Briefly ...
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bioRxiv - Genomics 2020Quote: ... Quantification and quality control of the final HiChIP library was conducted using a Bioanalyzer and KAPA quantification kit (Kapa Biosystems). Libraries underwent Next Generation Sequencing on a HiSeq 2500 generating 100 bp paired-ends.