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Citations for Roche :
4651 - 4700 of 7005 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The library amplification step included 6-7 PCR cycles and was performed using the KAPA Library Amplification kit (Roche). The final Hi-C library was purified using SPRIselect beads and the quality and size of the library (approximately 500 bp ...
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bioRxiv - Genetics 2021Quote: BAM files from previously published multi-region WES data (generated using the SeqCapEx Exome Enrichment Kit v3.0 (Nimblegen/Roche)) from nine sporadic fresh-frozen adenomas were available (Supplementary Table 2).11 Somatic variants were re-called using Mutect2 as above.
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bioRxiv - Microbiology 2021Quote: ... The 16S rRNA V3–V4 amplicon was amplified using a KAPA HiFi HotStart ReadyMix PCR Kit (KAPA BioSystems, USA). the amplicon PCR forward primer (5’-CCTACGGGNGGCWGCAG-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... 4 were purified according to the instructions of the manufacturer using the High Pure PCR Template Preparation Kit (Roche) followed by RNAse A digest and a final purification with the High Pure PCR Product Purification Kit (Roche).
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bioRxiv - Plant Biology 2021Quote: ... Library preparation and sequencing were performed by the iGenSeq genotyping and sequencing core facility (ICM Institute - Hôpital Pitié-Salpêtrière AP-HP, Paris, France) using KAPA HyperPrep Kits (Roche, Basel, Switzerland) and the NovaSeq 6000 sequencer (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... hygromycin B phosphotranferase (HYG) or blastacidin S deamidase gene (BSD) generated using the PCR DIG Probe Synthesis Kit (Roche). The blot was developed according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... The qPCR was performed using KAPA SYBR® FAST qPCR Kit Master Mix (2X) Universal (Kapa Biosystems; Massachusetts, USA). Protein expression at day 10 post transfection was analyzed by immunocytochemistry using the following antibodies and dilutions ...
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bioRxiv - Developmental Biology 2020Quote: ... TUNEL staining was performed according to respective manufacturer protocol using Terminal Deoxynucleotidyl Transferase kits (Invitrogen #10533065 or Roche #03333574001), dig-UTP (Roche ...
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bioRxiv - Genomics 2020Quote: A screenshot showing read visualization by IGV of the region were the Alu insertion occurred in the positive control sample (BRCA1) when using the SeqCap EZ Exome v3.0 kit from Roche for exome capture is presented in Figure 2 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100-500 ng of DNA were used to prepare libraries using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR ...
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bioRxiv - Biophysics 2021Quote: ... The troponin T detection kit (Elecsys® Troponin T Gen 5 STAT) and troponin T were purchased from Roche. 1X phosphate-buffered saline (PBS ...
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An optimized ChIP-Seq framework for profiling of histone modifications in Chromochloris zofingiensisbioRxiv - Genomics 2021Quote: RNA was converted into cDNA and made into sequence ready libraries with the KAPA RNA-Seq Kit (Kapa Biosystems). RNA-Seq libraries were sequenced with 150 bp single-end reads on a HiSeq 2500 ...
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bioRxiv - Cell Biology 2020Quote: RACE-cDNA was synthesized according to the manufacturer’s protocol for 5’/3’ RACE Kit 2nd generation (Roche; Cat.No. 03353621001). The 2648-bp cDNA was cloned into the NheI-XhoI site in pcDNA 3.1 plasmid to obtain pcDNA3.1-DR5-AS and was verified by sequencing ...
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bioRxiv - Developmental Biology 2022Quote: ... The library was enriched using 14 PCR cycles and quantified with Kapa NGS library quantification kit (Kapa Biosystems, KK4824) before pooling at a concentration of 20nM ...
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bioRxiv - Cancer Biology 2022Quote: ... Post-capture amplification was performed using the KAPA HiFi Hot Start PCR ReadyMix Kit (KAPA Biosystems, Catalogue no. KK2601). Post-capture amplified libraries were quality controlled and quantified using a Tapestation 2200 with the High Sensitivity reagents.
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bioRxiv - Molecular Biology 2022Quote: Southern blot analysis was performed using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche 11585614910) and the DIG Wash and Block Buffer Set (Roche 11585762001) ...
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Activation of the Integrated Stress Response overcomes multidrug resistance in FBXW7-deficient cellsbioRxiv - Cancer Biology 2022Quote: ... The U6-sgRNA cassette was then amplified by PCR using the KAPA HIFI Hot Start PCR kit (Roche, KK2502) and different tagged primers required for the subsequent Illumina sequencing ...
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bioRxiv - Microbiology 2022Quote: ... Library quality control and quantification were performed using a KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems, KK4873) and the 2100 Bioanalyzer equipped with a High Sensitivity DNA kit (Agilent ...
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bioRxiv - Genetics 2022Quote: ... Library preparation was performed with 1ug of sheared DNA using Kapa Biosystems Hyper Prep Kit (Roche, product number KK8504) dual index adapters (KAPA ...
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bioRxiv - Cancer Biology 2022Quote: 300 ng of total RNA was used for library preparation using the KAPA RNA HyperPrep kit with RiboErase (Roche), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Pax6a digoxigenin (DIG) antisense RNA probes were generated from linearised plasmids using an RNA labelling and detection kit (Roche) (Scholpp and Brand ...
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bioRxiv - Systems Biology 2022Quote: ... libraries for polyA+ RNA were prepared using KAPA Stranded RNA-Seq Kit for Illumina Platforms (KAPA Biosystems, Wilmington, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and RNA sequencing libraries were prepared using 400 ng of total RNA using the Kapa mRNA Hyperprep kit (Roche) at 1/3rd reaction volume using 14 cycles of PCR ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were also stained for β-galactosidase (β-gal) activity with the use of a staining kit (11828673001, Roche). Apoptotic cells were detected by TUNEL analysis with digoxigenin-labelled dUTP (S7105 ...
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bioRxiv - Plant Biology 2021Quote: ... The qPCR was run using KAPA SYBR® FAST qPCR Kit Master Mix on LightCycler® 96 instrument (Roche) as described previously described (Han et al. ...
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bioRxiv - Microbiology 2021Quote: ... The RNA-seq library was prepared from viral RNA extracts using the KAPA RNA HyperPrep Kit (Roche, Penzberg, Germany) and KAPA DI adaptors according to the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... DIG-labeled probes where generated using T7/SP6 transcriptions start sites and the digoxigenin RNA Labeling Mix kit (Roche) after manufactures instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and the concentration of indexed libraries was quantified following the protocol of the KAPA library quantification kit (Kapa Biosystems). For each donor libraries from all tissues were combined prior to sequencing and sequenced on the Illumina Hiseq 4000 system in 2 lanes.
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bioRxiv - Neuroscience 2021Quote: ... Expressed sequence tags (ChESTs; SourceBioScience) were used to generate in situ probes using a DIG RNA labeling kit (Roche). In situ hybridization was performed as described (Mauti et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... and PCR products were quantified fluorometrically using the KAPA SYBR FAST qPCR Master Mix (2X) Kit (KR0389, KAPA Biosystems).
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bioRxiv - Microbiology 2022Quote: ... After 24 h treatments were performed and ATP was measured using the ATP Bioluminescence Assay Kit CLSII from Roche. This assay exploits the fact that luciferase requires ATP to produce light ...
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bioRxiv - Microbiology 2022Quote: ... detection of bound conjugate was achieved by the use of an enhanced chemiluminescence assay (ECL) kit (Roche Applied Science). Images were acquired using a BIO-RAD ChemiDoc imaging system.
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bioRxiv - Genetics 2022Quote: ... Total Ty1-copia sequences labeled with DIG were prepared as probes using the PCR-DIG Probe Synthesis Kit (Roche). ImageJ and Calculator software (http://cels.uri.edu/gsc/cndna.html ...
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bioRxiv - Cell Biology 2022Quote: ... sequencing libraries were prepared by using a modified version of the KAPA HyperPrep library kit (KAPA Biosystems, Willmington, MA) and sequenced by a NovaSeq instrument ...
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bioRxiv - Pathology 2022Quote: The supernatant from cultured NRVMs was collected to determine the content of LDH via cytotoxicity detection kit (Roche, USA), following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... The prepared libraries were then quantified by qPCR using the Kapa SYBR Fast Illumina Library Quantification Kit (Kapa Biosystems) and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Bioengineering 2022Quote: ... The libraries were pooled to 10 nM and diluted for qPCR using the KAPA Library Quantification Kit (Kapa Biosystems). Subsequently the pooled libraries were normalized to 4 nM based on qPCR values ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR reaction was prepared using a LightCycler® 480 SYBR Green I Master kit (Roche Diagnostics, Mannheim, Germany) and the PCR was performed with a LightCycler® 480 Instrument II (384-well ...
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bioRxiv - Synthetic Biology 2020Quote: ... Washing and detection steps were carried out as outlined in the manufacturer’s protocol for the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche).
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bioRxiv - Genomics 2020Quote: ... was generated with KAPA Hyperprep Stranded mRNA-seq library kits (Roche Sequencing and Life Science, Kapa Biosystems, Wilmington, MA) and sequenced with a Novaseq 6000 using 150bp paired end reads.
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bioRxiv - Plant Biology 2020Quote: ... A 1 µg RNA was used to synthesize cDNA using a first-stand cDNA synthesis kit (Roche Diagnostics, Switzerland). The cDNA was normalized to 100 ng µl−1 and used for PCR analysis in a 10 µl reaction with gene-specific primers (5‘-CGTGGGATACCCTTACATGG and 5‘-CCCGATTATCTTCCACCAGA for ZmOMT1 ...
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bioRxiv - Immunology 2020Quote: ... genes of SARS-CoV-2 was performed using the LightMix Modular SARS and Wuhan CoV E-gene Kit (TIB MOBIOL, Synheselabor GmbH, Berlin, Germany) in a LightCycler 480 II system following the manufacturer’s recommendation (Roche Diagnostics International Ltd. ...
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bioRxiv - Genomics 2020Quote: ... Bisulfite converted DNA was then used as template for PCR amplification with KAPA HiFi HotStart Uracil kit (Roche, KK2801) using primers surrounding Site 1 in intron 8 of the Fto gene ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The pooled and purified library was quantified using the Universal KAPA Library Quantification kit for Illumina platforms (Roche, # KK4824). The pooled TempO-Seq® library was sequenced in-house using the NextSeq® 500/550 High Output Kits v2 (75 cycles ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was fragmented to 100-300bp and libraries were constructed using the KAPA HTP Library Kits (KAPA Biosystems). A single low input sample used the Swift Accel-NGS 2S DNA Library Kit (Swift BioSciences ...
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bioRxiv - Cancer Biology 2021Quote: ... and quantitative RT-PCR for library quantification was performed using the Kapa Library Quantification Kit (Roche Sequencing, Pleasanton, CA). The libraries were sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate about 40 million read pairs per sample.
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were prepared using a miniaturized version of the Kapa HyperPlus Illumina-compatible library prep kit (Kapa Biosystems). DNA extracts were normalized to 5 ng total input per sample using an Echo 550 acoustic liquid handling robot (Labcyte Inc) ...
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bioRxiv - Microbiology 2021Quote: ... shotgun library construction was performed for the extracted DNA using a KAPA Hyper Prep kit for Illumina (KAPA Biosystems), and sequencing of the library was performed on an Illumina MiSeq platform (MiSeq PE300) ...
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bioRxiv - Genetics 2020Quote: ... 400ng of total RNA (RIN >6) was used to generate polyA-selected libraries with Kapa mRNA HyperPrep kits (Roche) with indexed adaptors ...
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bioRxiv - Molecular Biology 2020Quote: ... The digested RNA samples were then analyzed by electrophoresis (5.5% PAGE with 8 M urea) and northern blotting using the DIG Northern Starter Kit (Roche). Poly(A ...