Labshake search
Citations for Roche :
4601 - 4650 of 7005 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... we constructed PE and SE libraries using the KAPA Hyper Library Preparation Kit (Kapa Biosystems, Woburn, MA, USA) and the Accel NGS 1S plus DNA kit (Swift BioSciences ...
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bioRxiv - Molecular Biology 2024Quote: The peptidyl-tRNAs in the PTS buffer were isolated by using a High Pure miRNA Isolation Kit (Roche), as described previously (54) ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA (2 µl) was added to the mixture with FastStart SYBR Green Master Mix kit (Roche, Basel, Switzerland) and specific primers SP_F 5’-GAC-CAG-TCG-AAC-GCA-CAT-TG-3’ and SP_R 5’-CGG-AGA-GGG-TTG-TTG-TGT-CT-3’ ...
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bioRxiv - Cell Biology 2024Quote: AST and ALT levels were determined with a Kit (AST: Cat# 04467493190, Roche; ALT: Cat No 04467388190, Roche) according to International Federation of Clinical Chemistry and Laboratory Medicine (IFCC ...
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bioRxiv - Molecular Biology 2024Quote: ... The yielded DNA was used to construct barcoded ChIP-seq libraries using the KAPA Hyper Pre Kit (Roche) according to the manufacturer’s instruction.
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bioRxiv - Neuroscience 2024Quote: ... quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems, Wilmington, MA, USA). Pooled libraries were paired-end sequenced on a High Throughput Run Mode flow cell with the V4 sequencing chemistry on an Illumina HiSeq 2500 platform (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... RNA libraries were prepared from 100 ng of total RNA using the Kapa mRNA Hyper Prep kit (Roche) and sequenced with a Novaseq SP v1.5 (100 cycles ...
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bioRxiv - Plant Biology 2024Quote: DNA library preparation was done according to the manufacturers protocol using the KAPA HyperPlus Kit (Roche, Basel, Switzerland). All library preparation was done using half of the recommended volumes ...
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bioRxiv - Plant Biology 2024Quote: ... size selected and amplified by 12–14 PCR cycles using a KAPA HiFi HotStart ReadyMix kit (Roche, KK2601). The PCR products were then purified using AMPure XP magnetic SPRI beads and quantified using a Bioanalyzer ...
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bioRxiv - Plant Biology 2024Quote: ... We conducted all qPCR reactions according to the instructions of the KAPPA SYBR FAST kit (Roche, Basel, Switzerland). All cDNA samples were diluted in a 1:100 ratio in nuclease-free water before analysis ...
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bioRxiv - Immunology 2024Quote: ... ∼200ng of genomic DNA were used for library construction using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR ...
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bioRxiv - Genomics 2024Quote: ... the quantification of DNA libraries was conducted with KAPA Library Quantification Kit ILLUMINA® Platforms (Roche, Cat No.KK48247) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: Genomic DNA (gDNA) was isolated from PBMCs via a High Pure PCR Template Preparation Kit (Roche, Penzberg, Germany). The concentration (ng/μL ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA libraries were generated from amplified samples using the KAPA Library Preparation Kit (Kapa Biosystems, Woburn, MA), and then fragmented to approximately 200 base pair (bp ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from PBMCs stored in RNAlater® with a High Pure RNA Isolation Kit (Roche) via a modified protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... The digoxingenin (DIG)-labeled RNA probes were synthesized using the meioc cDNA and DIG RNA-labeing kit (Roche), and the reagents and antibodies were listed in supplemental Table S6.
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed using SYBR green premix pro Taq HS qPCR kit with a LightCycler 96 instrument (Roche) and PCR was performed using 2×TransTaq-T PCR SuperMix ...
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bioRxiv - Bioengineering 2024Quote: ... lactate dehydrogenase (LDH) activity was measured in culture supernatants using a colorimetric detection kit (Roche, Diagostics, Meylan, France).
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bioRxiv - Cancer Biology 2024Quote: DNA was quantified by qPCR using a KAPA library quantification kit (Cat. #KK4835; Kapa Biosystems, Wilmington, Massachusetts, USA). The supplied primer premix was first added to the supplied KAPA SYBR FAST master mix ...
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bioRxiv - Cancer Biology 2024Quote: ... The total RNAs were converted into individually barcoded polyadenylated mRNAseq libraries with the Kapa HyperPrep mRNA kit (Roche). Libraries were barcoded with Unique Dual Indexes (UDI’s ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were then pooled at equimolar concentrations and quantified by qPCR using a KAPA library quantification kit (Roche). Sequencing was performed on a NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Genetics 2024Quote: ... Real-time PCR was done using KAPA SYBR FAST qPCR Master Mix (2X) Kit (Kapa Biosystems, Wilmington, MA) on a CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... kit was used in all qPCR eDNA runs on 96-well reaction plates (Roche Diagnostics GmbH, Mannheim, Germany) with the parameters shown in Fig 3 ...
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bioRxiv - Genetics 2024Quote: ... Probes were labeled using the DIG High-Prime DNA Labeling and Detection Starter Kit I (Cat #: 11745832910; Roche). Repeat tract estimation was performed as previously described (Southern blot analysis of repeat expansion size).
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bioRxiv - Evolutionary Biology 2024Quote: ... BAC arrayed library filters were screened with a DIG-labeled Pfbra probe (PCR DIG probe synthesis kit, Roche) following the hybridization protocol (DIG high prime DNA labeling and detection starter kit II ...
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bioRxiv - Cell Biology 2024Quote: Libraries were constructed from 600ng of DNA using the KAPA Hyper PCR-free Library Prep Kit (KAPA Biosystems) on a SciClone NGS instrument (Perkin Elmer) ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labelled antisense and sense RNA probes were synthesized by in vitro transcription using DIG-labelled UTP according to the manufacturer’s instructions (DIG RNA labelling kit, Roche). Primers used were as follow ...
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bioRxiv - Developmental Biology 2021Quote: ... Pax3 digoxigenin (DIG) - labeled antisense riboprobes were transcribed from linearized gene-specific probes (PCR DIG probe Synthesis Kit, Roche). WISH experiment was performed as follows ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was performed on a Roche 480 LightCycler using the LightCycler 480 DNA SYBR Green I Master kit (Roche). In all cases ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq libraries were generated using the KAPA HyperPrep Kit with RiboErase (HMR) and KAPA dual-indexed adapters (Roche Sequencing and Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted by combining phenol-chloroform–isopropanol treatment using the High Pure RNA Isolation Kit (Roche, Germany) (101) ...
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bioRxiv - Genomics 2020Quote: ... The sheared DNA was used for paired-end library preparation with a KAPA LTP Library Preparation Kit (KAPA Biosystems). The optimal numbers of PCR cycles for individual libraries were determined with a Real-Time Library Amplification Kit (KAPA Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by quantitation using real-time PCR using the KAPA Library Quant Kit for Illumina (KAPA Biosystems; Cat#KK4835). Libraries were then pooled and sequenced (1×75bp ...
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bioRxiv - Cancer Biology 2020Quote: One microgram of total RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche) with the oligonucleotide (dT)18 primer according to the manufacturer’s instructions (30,34) ...
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bioRxiv - Genomics 2020Quote: ... Total RNA sequencing libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase for Illumina Platforms (KAPA Biosystems). Briefly ...
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bioRxiv - Genomics 2021Quote: ... RNA-sequencing (RNA-seq) libraries were generated from 300ng RNA using the KAPA RNA HyperPrep Kit with RiboErase (Roche) and sequenced with NovaSeq 6000 (2×150 bp ...
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bioRxiv - Cell Biology 2021Quote: ... A cDNA library was made from Oligo(dT)12–18 as described in the manufacturer’s protocol (Transcriptor First Strand cDNA Synthesis Kit, Roche). Quantitative PCR was performed using Quantifast SYBR Green PCR Kit (204052 ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared using 50 ng of DNA with the KAPA HyperPlus Kit as per the manufacturer’s protocol (Roche). The libraries were sequenced with a paired-end read length of 150bp ...
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bioRxiv - Microbiology 2021Quote: ... The final pool was quantified by qPCR with Kapa Library Quant Kit for Illumina Platforms (KapaBiosystems, Roche, Cat. # KK4824) on an AriaMX Real Time PCR System (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: Total nucleic acid was extracted from clinical isolates using the AMPLICOR® Respiratory Specimen Preparation Kit (Roche, Basel, Switzerland) and purified with 1.8X AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... Samples were further processed using the Kapa LTP Library Preparation Kit for Illumina Platforms (Kapa Biosystems, Part Number KK8232) and indexed with the NEBNext Multiplex Oligos for Illumina 96 Unique Dual Index Primer Pairs (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... Prepared libraries were quantified using KAPA Biosystem’s next-generation sequencing library qPCR kit (Kapa Biosystems / Roche AG, Basel, Switzerland) and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Bioengineering 2020Quote: ... Prepared libraries were quantified using KAPA Biosystem’s next-generation sequencing library qPCR kit (Kapa Biosystems / Roche AG, Basel, Switzerland) and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Neuroscience 2021Quote: ... The eaat2a target site was PCR amplified with a fast-cycling polymerase (KAPA2G Fast HotStart PCR kit, KAPA Biosystems) (primers ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was performed on a Roche 480 LightCycler using the LightCycler 480 DNA SYBR Green I Master kit (Roche). In all cases ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA synthesis was performed with equal RNA input using the Transcriptor First Strand cDNA synthesis kit (Roche Molecular Biochemicals). Quantitative PCR was performed using C1000 Touch Thermal Cycler (BioRed ...
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bioRxiv - Neuroscience 2020Quote: ... The complementary DNA (cDNA) was synthesized using a transcriptor high fidelity cDNA synthesis kit (Roche Applied Science, Penzberg, Germany). Each prepared sample used 20 µL of the SYBR green qPCR reaction kit (Roche Applied Science) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg poly[d(I-C)] and 0.1 μg poly-Lysine (based on DIG Gel Shift Kit, 2nd Generation, Roche) for 15 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... pooled and quantified by qPCR with the KAPA Library quantification kit for Illumina platforms (Kapa Biosystems, Wilmington MA, USA) on a StepOnePlus qPCR system (Thermo-Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from the culture supernatant after each passage using a High Pure Viral RNA kit (Roche Diagnostics). For deep sequencing of the S1/S2 cleavage site of the viral S gene ...