Labshake search
Citations for Roche :
401 - 450 of 577 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... Full-length RNA was made into libraries using the KAPA stranded mRNA library prep kit (Roche, Nutley, NJ) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... Full length cDNA was then converted into an Illumina sequencing library using the Kapa Hyper Prep kit (Roche). In brief ...
-
bioRxiv - Cancer Biology 2023Quote: Low-pass WGS libraries were created using the Kapa Biosystems Hyper Prep kit (KAPA Biosystems, Capetown, South Africa) using 11.8 ng/ml of G4_1 ...
-
bioRxiv - Genetics 2023Quote: ... The Yale Center for Genome Analysis prepared libraries using polyA-selection (Roche Kapa mRNA Hyper Prep Cat #KR1352) and sequenced them on an Illumina NovaSeq 6000 to generate paired 150 bp reads.
-
bioRxiv - Molecular Biology 2023Quote: ... Libraries preparation were performed with a Kapa Hyper Prep Kits with KAPA Library Amplification Primer Mix (Roche, 07962347001), quantified by Qubit (Invitrogen) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 100 ng of DNA were used to prepare libraries using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR ...
-
bioRxiv - Evolutionary Biology 2023Quote: DNA libraries for all samples were prepared using a KAPA Hyper Prep kit and PCR-free protocol (Roche). For each genotype ...
-
bioRxiv - Neuroscience 2024Quote: ... RNA libraries were prepared from 100 ng of total RNA using the Kapa mRNA Hyper Prep kit (Roche) and sequenced with a Novaseq SP v1.5 (100 cycles ...
-
bioRxiv - Immunology 2024Quote: ... ∼200ng of genomic DNA were used for library construction using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR ...
-
bioRxiv - Cell Biology 2024Quote: Libraries were constructed from 600ng of DNA using the KAPA Hyper PCR-free Library Prep Kit (KAPA Biosystems) on a SciClone NGS instrument (Perkin Elmer) ...
-
bioRxiv - Biochemistry 2020Quote: ... The gel piece was vacuum dried and treated with trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche sequencing grade) at 37°C for 16 hours ...
-
bioRxiv - Biochemistry 2020Quote: ... Ganglia were mechanically disrupted with a pipette tip until obtaining a single cell suspension in culture media supplemented with DNAse I grade II at final concentration 3mg/ml (Roche, Cat# 104159), which was centrifuged at 1300 rpm (302 rcf ...
-
bioRxiv - Neuroscience 2020Quote: ... 35% of the UTP in each reaction was replaced with either Fluorescein-12-UTP (Roche), Digoxigenin-11-UTP (Roche ...
-
bioRxiv - Cell Biology 2022Quote: ... the eluted DNA was amplified for 11-12 cycles with Kappa HiFi Ready-mix (Roche) and custom Nextera PCR primers ...
-
bioRxiv - Biochemistry 2021Quote: ... the proteins were detected using an HA-HRP antibody (12-013-819; 1:3,500; Roche).
-
bioRxiv - Microbiology 2021Quote: ... and 25 Abs260 ribosome units (1 A260 = 12 μg/μL) were digested with MNase (Roche). Digested samples were carefully loaded onto sucrose gradients and centrifuged at 124,700 × g for 8 hr at 4°C ...
-
bioRxiv - Genetics 2022Quote: ... PCR was performed in 12 μL containing 6 μL Kapa HRM-Fast Master mix (Roche), supplemented with 1.5 mM MgCl2 ...
-
bioRxiv - Neuroscience 2024Quote: ... incubated with anti-DIG-POD antibody (Roche; 1:1000 in TNB; 12 hr, 4 °C), and washed in TNT (3 × 20 min ...
-
bioRxiv - Genomics 2023Quote: ... 12 μL of KAPA_mix [1X KAPA HiFi HotStart buffer Ready Mix (Roche, Cat. No KK2601), 0.5 μM LIDAR_preamp_f ...
-
bioRxiv - Bioengineering 2024Quote: ... amplified with 12 rounds of PCR using KAPA HiFi DNA polymerase (Roche cat. no. 07958935001) and purified with a DNA Clean & Concentrator column (Zymo cat ...
-
bioRxiv - Cancer Biology 2020Quote: ... 100-500 ng of DNA were used to prepare libraries using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR ...
-
bioRxiv - Genetics 2022Quote: ... Library preparation was performed with 1ug of sheared DNA using Kapa Biosystems Hyper Prep Kit (Roche, product number KK8504) dual index adapters (KAPA ...
-
bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were prepared using a miniaturized version of the Kapa HyperPlus Illumina-compatible library prep kit (Kapa Biosystems). DNA extracts were normalized to 5 ng total input per sample using an Echo 550 acoustic liquid handling robot (Labcyte Inc) ...
-
bioRxiv - Microbiology 2021Quote: ... shotgun library construction was performed for the extracted DNA using a KAPA Hyper Prep kit for Illumina (KAPA Biosystems), and sequencing of the library was performed on an Illumina MiSeq platform (MiSeq PE300) ...
-
bioRxiv - Molecular Biology 2020Quote: ... the libraries were prepared from 500 pg of immunoprecipitated DNA fragments using the KAPA Hyper Prep Kit (KAPA Biosystems). The libraries were applied to Single-end sequencing for 93 cycles on HiSeq2500 (Illumina) ...
-
bioRxiv - Genetics 2020Quote: ... A total of 80 ng of purified amplicon was inputted in the KAPA Hyper Prep (Roche Sequencing, CA, USA) library preparation according to manufacturer’s recommendations ...
-
bioRxiv - Genomics 2020Quote: ... The fragmented DNA was then ligated with dual-indices using a KAPA Hyper prep PCR-free library kit (Roche) according to the manufacturer’s directions ...
-
bioRxiv - Genomics 2021Quote: ... Library preparation was carried out with 500 ng sheared DNA using Kapa Hyper library prep kit (Roche, Basel, Switzerland). Barcodes were added using the Illumina UD 96 index kit (Illumina) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Sequence libraries were prepared starting with 0.5 μg of genomic DNA using the KAPA Hyper Library Prep (KAPA Biosystems). We sequenced genomic DNA on the Illumina NovaSeq (150 bp x 2 paired-end ...
-
bioRxiv - Plant Biology 2024Quote: We fragmented 700 ng of high-molecular-weight DNA using a Covaris S220 sonicator and a WGS library was generated for both species using the KAPA Hyper Prep kit with a PCR-free protocol according to the manufacturer’s instructions (Roche). We applied final size selection using a 0.7-fold ratio of AMPureXP beads ...
-
bioRxiv - Genetics 2023Quote: ... Sequencing libraries were prepared using 100 ng fragmented DNA with the KAPA HyperPrep Library Prep Kit (Roche, Indianapolis, IN) and NEXTFLEX UDI barcodes (PerkinElmer ...
-
bioRxiv - Immunology 2023Quote: ... Poly(A)-enriched next-generation sequencing library construction was performed using the KAPA mRNA Hyper Prep Kit (KAPA Biosystems) with 100 ng of input total RNA and 15 amplification cycles according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR ...
-
bioRxiv - Genomics 2022Quote: ... Libraries were constructed from 10 ng purified DNA using the High Throughput Library Prep Kit from KAPA Biosystems (KK8234) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: NGS libraries from DRIP DNA and inputs were constructed according to the manufacturer’s instructions (KAPA Hyper Prep Kit: Roche, cat ...
-
bioRxiv - Cancer Biology 2024Quote: ... 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems, #KK8504) using 8 cycles of PCR ...
-
bioRxiv - Evolutionary Biology 2024Quote: A PCR free library was prepared following the Kapa Hyper Prep Kit procedure provided by Roche (Roche, Basel, Switzerland). The library was sequenced on a paired-end mode with an Illumina NovaSeq 6000 instrument (Illumina ...
-
bioRxiv - Cell Biology 2020Quote: ... The solution was refreshed one time before the addition of Liberase TM research grade (final concentration 0.038 mg/mL (Roche Diagnostics, GmbH, Mannheim, Germany)) and Elastase from porcine pancreas (final concentration 0.01 mg/mL (Bio-Connect B.V. ...
-
bioRxiv - Molecular Biology 2021Quote: ... sections were fixed with 4% PFA (PierceTM, 28908) in PBS for 15 minutes at room temperature and permeabilized with PCR grade recombinant Proteinase K (Roche Applied Science, 3115887001) for 30 minutes at 37 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... The bisulfite converted libraries were amplified for 12 cycles with KAPA HiFi HotStart Uracil+ ReadyMix (Roche). The amplified libraries were cleaned up with AMPure beads (0.8X ratio ...
-
bioRxiv - Biochemistry 2021Quote: The LLPS model in solution was made by (PR)12 peptide and poly-rA RNA (Roche) in water or PBS18 ...
-
bioRxiv - Cell Biology 2024Quote: ... and incubated in digestion solution (3 mg/mL collagenase D [Roche] in DMEM/F-12 [Gibco]) for 45 min at 37 °C with intermittent vortex mixing to dislodge soft tissues ...
-
bioRxiv - Molecular Biology 2024Quote: ... The 12-μL PCR mixture contained 10 μL of KAPA PCR Ready Mix (Kapa Biosystems, Japan), 0.5 μL of 10 μM of each primer ...
-
bioRxiv - Cell Biology 2020Quote: ... with mRNA enrichment from 100 ng of total purified RNA and Illumina sequencing libraries preparation performed using Kapa stranded mRNA Hyper Prep (Roche Sequencing Solutions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The Illumina library construction and sequencing were performed at Duke University using KAPA Hyper Prep kits (Kapa Biosystems, Wilmington, MA) and the Illumina NovaSeq 6000 platform (paired-end 150 bp reads) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Sequencing libraries were constructed from 1 ng of immunoprecipitated and input DNA using the KAPA Hyper Prep kit (KAPA Biosystems) and NEXTflex ChIP-seq barcodes (Bio Scientific).
-
bioRxiv - Evolutionary Biology 2022Quote: We prepared libraries for each individual using the Kapa Hyper Prep library preparation kit (Kapa Biosystems Inc., Wilmington, MA, USA) and enzymatic fragmentation of DNA ...
-
bioRxiv - Cancer Biology 2022Quote: ... Large scale preps of bacteria cultures containing the sgRNA plasmid library were harvested using the Genopure plasmid maxi kit (Roche). SIDP library was packaged in lentiviral particles by large scale co-transfection of HEK293T cells with CELLECTA ready-to-use packaging plasmid (Cellecta – cat.no CPCP-K2A ...
-
bioRxiv - Bioengineering 2022Quote: ... DNase-treated RNA was converted into barcoded polyadenylated mRNA libraries using the Kapa Hyper Stranded mRNA Sample Prep Kit (Roche). Libraries were barcoded with unique dual indexes to prevent index switching and adaptor-ligated double-stranded cDNAs were PCR-amplified for eight cycles with KAPA HiFi DNA Polymerase (Roche) ...
-
bioRxiv - Microbiology 2021Quote: ... Between 150 and 200 ng of genomic DNA was sheared acoustically and HiSeq sequencing libraries were prepared using the KAPA Hyper Prep Kit (Roche). PCR amplification of the libraries was carried out for 10 cycles ...