Labshake search
Citations for Roche :
501 - 550 of 577 citations for Superose 12 Prep Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... adaptor-ligated DNA was enriched by PCR for 12 cycles using KAPA HiFi HotStart Uracil+ Kit (KAPA Biosystems), purified with Agencourt beads (Agilent ...
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bioRxiv - Plant Biology 2024Quote: ... size selected and amplified by 12–14 PCR cycles using a KAPA HiFi HotStart ReadyMix kit (Roche, KK2601). The PCR products were then purified using AMPure XP magnetic SPRI beads and quantified using a Bioanalyzer ...
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bioRxiv - Cell Biology 2020Quote: ... 4 µg of total RNA was used to generate libraries using of stranded poly(A) mRNA-Seq library with the KAPA stranded mRNA Library prep kit (KAPA Biosystems, KK8421). Libraries concentration was quantified with Qubit dsDNA HS (High Sensitivity ...
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bioRxiv - Genomics 2020Quote: ... a total of 100 ng DNA from each bacterial strain was used according to KAPA Hyper Prep kit (KAPA Biosystems, MA, USA) at the Georgia Genomics and Bioinformatics Core (GGBC) ...
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bioRxiv - Plant Biology 2021Quote: ... The sheared DNA fragments were end-repaired and deoxyadenosine-tailed in a 60 µL volume with 3 µL End Repair & A-Tailing Enzyme Mix and 7 µL End Repair & A-Tailing Buffer using a KAPA Hyper Prep Kit (KAPA Biosystems, USA) according to the manufacturer instructions (20°C 30 min ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was then provided to the Functional Genomics Laboratory at UC Berkeley where RNA-seq libraries were prepared by Oligo dT enrichment followed by a stranded Illumina library prep protocol with the KAPA mRNA HyperPrep kit (Kapa Biosystems, KK8580). Libraries were checked for quality on an AATI Fragment analyzer (Agilent ...
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bioRxiv - Genetics 2021Quote: The bone libraries were prepared in a dedicated low copy DNA laboratory using the KAPA Hyper Prep Kit (Roche Sequencing, Wilmington, MA). Library preparation followed the manufacturer’s recommendations using 15 µM 8-bp dual-indexed adapters ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library preparation was carried out using KAPA Hyper Prep and HiFi HS Library Amplification kits (F. Hoffmann-La Roche AG, Basel, Switzerland) and with iTru i5 and i7 dual-indexing primers (BadDNA ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 ng of purified DNA for each IP and input sample were used for library preparation with the KAPA Hyper Prep Kit (Kapa Biosystems KR0961). TAZ and Combo treatments required two IPs per biological replicate to attain enough material for library preparation ...
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bioRxiv - Cancer Biology 2023Quote: ... a maximum of 1 μg of sheared DNA was used for library preparation using the KAPA HTP Prep Kit (KAPA Biosystems, KK8234) following manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Whole genome library preparation (without duplex) Next generation sequencing libraries were generated using a double-stranded preparation kit (Kapa Hyper Prep Kit, Roche, cat#KK8502). Full-length adapters (IDT TruSeQ UDI plate ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA samples were submitted to the Dana-Farber Molecular Biology Core Facility for mRNA sequencing using the Roche Kapa mRNA Hyper Prep kit (Roche, Basel, Switzerland) with samples sequenced on an Illumina NextSeq or NovaSeq ...
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bioRxiv - Immunology 2024Quote: ... 7.4-10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems cat. no. KK8504) using 8 cycles of PCR ...
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bioRxiv - Evolutionary Biology 2024Quote: A PCR free Illumina library (based on the same HMW extraction) was prepared using the Kapa Hyper Prep Kit (Roche, Basel, Switzerland), following manufacturer instructions ...
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bioRxiv - Plant Biology 2020Quote: One µg of 3’ RACE amplicons were diluted to 20 ng/µl in elution buffer and used as input in a modified KAPA Hyper Prep library production protocol (Kapa Biosystems, MA, USA) to generate libraries for Illumina sequencing ...
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bioRxiv - Paleontology 2022Quote: DNA library construction for whole-genome sequencing was performed using the KAPA Hyper Prep Kits for Illumina® NGS platforms (KAPA Biosystems, KK8504). Adapters and 8bp index oligos purchased from IDT® (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2020Quote: ... 500 μg total RNA was used to prepare polyA-enriched RNA-seq libraries using the KAPA mRNA Hyper Prep Kit (Roche, ref. KK8581/KK8581). Those libraries were prepared separately for each sample with 11 amplification cycles ...
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bioRxiv - Microbiology 2021Quote: ... The purified DNA was used for Illumina sequencing library construction using a KAPA Hyper Prep Kit (for Illumina) (KAPA Biosystems, Wilmington, MA, USA). Sequencing was performed on an Illumina HiSeq 2500 platform (San Diego ...
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bioRxiv - Immunology 2023Quote: ... and adapter-ligated using the KAPA PCR-free Hyper Prep Kit in combination with KAPA Unique Dual-Indexed Adapters (F. Hoffmann-La Roche AG, Basel, Switzerland). Adapter-ligated libraries were purified by AMPure XP bead cleanup ...
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bioRxiv - Genomics 2023Quote: ... DNA-Seq libraries were prepared from 1 mg of DNA extracted from young leaves using the Kapa LTP library prep kit (Kapa Biosystems, MA, USA). After quantity and quality evaluation with the High Sensitivity chip of a Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We prepared dual- indexed libraries (Glenn et al. 2019) for targeted enrichment using the KAPA Hyper Prep Library Kit (KAPA Biosystems, Wilmington, MA) following manufacturer’s protocols ...
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bioRxiv - Evolutionary Biology 2024Quote: ... A volume of 50 µl (1 µg) of sheared DNA from each sample was used for library preparation using a KAPA Hyper Prep Illumina Kit (KAPA Biosystems, Wilmington, MA) and 48 barcoded adaptors (Bioo Scientific ...
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bioRxiv - Genomics 2024Quote: Library preparation was carried out at the University of Michigan Advanced Genomics Core using the KAPA mRNA Hyper Prep Kit (Roche, Wilmington, MA, USA) with dual indexing adapters ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared dual-indexed libraries with custom indexes (Glenn et al. 2019) and the Kapa Biosystems Hyper Prep Plus library preparation kit (F. Hoffmann-La Roche AG, Basel, Switzerland) following the manufacturer’s protocol at one-half volume.
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bioRxiv - Cell Biology 2021Quote: ... A cDNA library was made from Oligo(dT)12–18 as described in the manufacturer’s protocol (Transcriptor First Strand cDNA Synthesis Kit, Roche). Quantitative PCR was performed using Quantifast SYBR Green PCR Kit (204052 ...
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bioRxiv - Microbiology 2020Quote: ... Reaction mixtures for each sample (24 µl) included 12 µl KAPA HiFi HotStart ReadyMix (2X) (KAPA Biosystems, Boston, Massachusetts), 1.5 µl NanoPure water ...
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bioRxiv - Cell Biology 2021Quote: ... were transfected into COS-7 cells (14,000-17,000 cells/cm2 in 12-well plate) for 24 h using X-tremeGENE HP DNA (Roche, #06366236001). Cells were incubated for 7 min at 37 °C with either 4 μg.mL-1 rabbit anti-γ2 Ex2 or 1:50 serum rabbit anti-γ8 Ex1 antibodies prior to fixation ...
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bioRxiv - Cell Biology 2020Quote: ... 50 ng of nick translated probe per coverslip was combined with 12 μg of human Cot-1 DNA (Roche), 10 μg salmon sperm ssDNA (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... containing 25 Abs260 ribosome units (1 A260 = 12 μg/μL) [5] were digested with 60 U of MNase (Roche) and 60 U of SUPERase·In (Ambion ...
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bioRxiv - Cancer Biology 2023Quote: ... The medium from each well was removed and replaced with fresh medium containing 10 µl of 12 mM MTT as per the manufacturer’s instruction (Roche). After 4 h of incubation ...
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bioRxiv - Plant Biology 2024Quote: ... on a rocker with horseradish peroxidase (HRP)-conjugated HA antibody (Roche, Indiana, USA, Cat. No. 12 013 819 001). Membranes were washed three times in 1X Tris-Buffered Saline (TBS ...
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bioRxiv - Genomics 2024Quote: Genomic DNA (5∼8 μg) for Nanopore sequencing was end-repaired and ligated via a KAPA Hyper Prep Kit (Cat#KR0961, Kapa Biosystems, Wilmington, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... mixed with alignment oligos and sample tracking control oligos (Nimblegen Hybridisation and Sample Tracking Control Kits) and hybridised to a 12×135k HD2 expression array (Roche Nimblegen ...
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bioRxiv - Plant Biology 2021Quote: ... 100 µl starch degradation mix (16.8 units ml-1 amyloglucosidase [#10102857001; Roche, Mannheim, Germany] and 12 units ml-1 α-amylase [#10102814001; Roche, Mannheim ...
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bioRxiv - Genomics 2023Quote: ... and incorporation of sample-specific multiplex indices before PCR amplification (12 cycles) according to the manufacturer’s protocol (Kapa Biosystems, USA). Size distribution and molarity of amplified cDNA libraries were assessed via the Bioanalyzer High Sensitivity DNA Assay (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... the libraries were amplified by 12-rounds of indexing PCR (2X KAPA HiFi Hot Start Ready Mix, Roche, cat.no. KK2601). Library quality was assessed using Bioanalyzer DNA High Sensitivity chip (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Zwittergent 3-12 (N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate) and protease inhibitor (Complete, Roche Applied Science)) for 2 h at 0°C (ref A) ...
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bioRxiv - Cancer Biology 2023Quote: ... glands were minced into paste and incubated in 5 mL DME/F-12 medium (HyClone, #SH30023.1) with 2 mg/mL collagenase A (Roche #10103578001), 100 units/mL hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Cells from a well of 12-well plate were lysed on ice in 80 µL RIPA buffer (Thermo) supplemented with protease inhibitor cocktail (Roche) and Benzonase (Novagen ...
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bioRxiv - Immunology 2024Quote: The retroviral recombination substrate pINV-12/23 was introduced into primary leukemic cells utilizing X-treme GENE HP DNA Transfection Reagent (Roche). Recombina-tion efficiency of pINV-12/23 was evaluated through flow cytometry analysis for mouse CD90 (mCD90 ...
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bioRxiv - Cancer Biology 2023Quote: ... or OSU-ERb-12 (100nM) + Enza (1uM) for 48h and processed as previously(24) reported using Annexin-V-FLUOS staining kit (Roche) and Propidium Iodide ...
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bioRxiv - Microbiology 2022Quote: ... the ligated product was amplified by 12 PCR cycles using the Kapa Hifi Hotstart NGS library amplification kit (Kapa Biosystems), followed by purification with 0.6×AMPure XP ...
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bioRxiv - Cancer Biology 2023Quote: ... The 12 and 32 libraries were equimolarly pooled and subjected to qPCR quantification using the KAPA library quantification kit (Roche). Sequencing was carried out on a NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... dissected in ice-cold PBS and then digested with 4mg/ml Collagenase Type IV/ 0.2mg/ml DNase I /10% FCS/PBS under constant shaking with 700 rpm at 37°C for 12-15 min (Collagenase Type IV, Cat#: 17104-019, Gibco; DNase I, Cat#: 10104159001, Roche). Samples were filtered using a 70µm nylon filter and then washed twice with FACS buffer (0.5% FCS/2mM EDTA in PBS ...
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bioRxiv - Immunology 2024Quote: ... ATP concentration in cell lysates was measured in a FB 12 Luminometer (Berthold detection systems) using the ATP Bioluminiscence Assay Kit CLS II (Roche).
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bioRxiv - Immunology 2024Quote: ... Libraries were amplified over 12 cycles in thermocycler using KAPA HiFi HotStart ReadyMix and 5µM Pre-LM-PCR Oligo 1&2 (Roche, Switzerland). Following amplification another 3-step adapter removal with AMPure XP beads was carried out ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft OS tumors were harvested and microdissected 12 d after 143B OS cell inoculation and digested with collagenase type I and II (1 mg/ml; Roche) digestion for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... clean tracheas were excised longitudinally and then incubated in Ham’s F-12 pen-strep with 1.5 mg/mL of pronase (Roche Molecular Biochemicals) for 16 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples (containing 20 μg of total protein) were mixed with loading buffer and separated on 12 % SDS-PAGE gels and transferred to PVDF membranes (Roche, Switzerland). After blocking with Tris-buffered saline with 0.1% Tween 20 and 5 % nonfat dry milk ...
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bioRxiv - Cancer Biology 2020Quote: ... Two milligrams recombinant MYC and 12 μl T7-HCF-1VIC were rotated overnight at 4°C in Kischkel buffer + PIC (Roche 05056489001). Anti-FLAG M2 Affinity Gel (Sigma-Aldrich) ...