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Citations for Roche :
401 - 450 of 881 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The DIG-labeled probes were hybridized to the membrane in DIG Easy Hyb hybridization buffer (Roche #11603558001) at 49°C for overnight ...
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bioRxiv - Neuroscience 2021Quote: ... The probes were then labeled using a digoxigenin (DIG) RNA labeling Kit (Roche Diagnostics Corp., Indianapolis, IN). After pre-hybridization ...
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bioRxiv - Physiology 2022Quote: ... Detection of DIG-labeled probes was performed by incubating with anti-DIG antibody (1:2000, Roche #11093274910) in 0.1% PBST overnight at 4° C ...
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bioRxiv - Developmental Biology 2020Quote: Riboprobes were made using T3 and T7 polymerases and a digoxigenin-labeled ribonucleotide mix (Roche, Cat.# 11277073910) using PCR templates ...
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bioRxiv - Molecular Biology 2019Quote: ... The Bar amplified fragment (Table 1) labeled by DIG high primer DNA labeling (Roche, Cat. No. 11585614910) and purified using a high pure PCR product purification kit (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... and digoxigenin-labeled RNA probes were transcribed using a digoxigenin-UTP in vitro transcription labeling mix (Roche) and either T7 or T3 RNA polymerase (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... and used as template to generate DIG-labeled in situ probes with SP6/T7 polymerases (Roche, # 10999644001). Whole-mount in situ hybridization was done according to Lécuyer et al ...
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bioRxiv - Molecular Biology 2022Quote: ... probes were digoxigenin-(DIG) labeled by using the DIG RNA Labeling Kit (Roche Co. Ltd, Basel, Switzerland). The female flower buds collected at 1 DAP were infiltrated with the FAA fix solution (90 ml of 50% (v/v ...
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bioRxiv - Cell Biology 2022Quote: Digoxin-labeled RNA probes were in vitro transcribed as per manufacturers’ instructions (DIG RNA labeling Mix, Roche; T7 polymerase ...
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bioRxiv - Developmental Biology 2022Quote: ... opilio used sense (control) and antisense RNA probes labeled with DIG RNA labeling mix (Roche, Basel, Switzerland) and followed published protocols (Sharma et al ...
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bioRxiv - Molecular Biology 2023Quote: ... DIG-labeled TER and alpha-tubulin probes were generated using the PCR DIG Probe Synthesis Kit (Roche) and used in the hybridization step (S1 Table ...
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bioRxiv - Developmental Biology 2023Quote: ... A DIG-labeled antisense RNA probe targeting lefty1 was synthesized using a DIG Probe Synthesis Kit (Roche). NBT/BCIP/Alkaline phosphatase-stained embryos were dehydrated in methanol before clearing and imaging in 2:1 benzyl benzoate:benzyl alcohol (BBBA ...
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bioRxiv - Cell Biology 2023Quote: ... RNA probes were transcribed and labeled with either FITC (fluorescein isothiocyanate) or DIG (digoxigenin) NTPs (Roche Cat#).
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bioRxiv - Genomics 2024Quote: ... The probe was labeled with alpha-32P CTP using the High Prime DNA labeling kit (Roche, #11585584001). The labelled probe was purified on a G50 sephadex column (Cytiva ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were first purified with a midiprep kit (Roche) and were transfected into HEK293T cells with a five-plasmid system to produce lentivirus with a very high titer of ~107 CFU/ml ...
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bioRxiv - Genomics 2021Quote: ... The purified DNA was quality-controlled using qPCR (Roche) and Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... using bovine serum albumin and purified calf histones (Roche) standards and purity examined by SDS-PAGE and Coomassie staining (>95% pure ...
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bioRxiv - Neuroscience 2020Quote: ... Probes were purified using Mini Quick Spin Columns (Roche). In-situ hybridization reaction was performed as described elsewhere (Thomas-Jinu & Houart ...
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bioRxiv - Genomics 2022Quote: ... PCR reactions were purified using 1.7×KAPAPure beads (Roche). Libraries were checked for quality and concentration using the DNA High-Sensitivity TapeStation assay (Agilent Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... SPRI-bead purified with KapaPure beads (Kapa Biosystems/Roche), quantified via qPCR with a Kapa Library Quant kit (Kapa Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... SPRI-bead purified with KapaPure beads (Kapa Biosystems/Roche), quantified via qPCR with a Kapa Library Quant kit (Kapa Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... the polyclonal murine anti N-terminal MSPDBL2 serum and a rat monoclonal α-HA antibody (Roche 3F10) were used ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated for at least 1 h with a 1:1000 dilution of a high affinity anti-HA antibody from rat IgG1 (#11867423001, Roche Applied Science, Penzberg, Germany). Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795 ...
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bioRxiv - Developmental Biology 2021Quote: ... DIG-labeled antisense or sense probes were synthesized using T7 or T3 RNA polymerase (Roche, 10588423 and 11031171001) and Dig RNA Labeling Mix (Roche ...
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bioRxiv - Immunology 2019Quote: ... In vitro biotin-labeled RNAs (lncAVAN and its antisense RNA) were transcribed with biotin RNA labeling mix (Roche) and T7 RNA polymerase (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... DIG-labeled RNA probes (prepared according to the protocol provided by Roche, Cat. No. 11 277 073 910) or radiolabeled DNA probes and RNA probes were used (Table S5) ...
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bioRxiv - Neuroscience 2020Quote: ... Digoxigenin (DIG)-labeled probes were synthesized with DIG RNA labeling kit version 12 (SP6/T7) (Roche, Mannheim, Germany). Biotin-labeled probes were synthesized by substitution of DIG RNA labeling mix with biotin RNA labeling mix (Roche ...
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bioRxiv - Biophysics 2020Quote: ... The 3′ end of the 1,882-nt DNA handle was labeled with dig-ddUTP using terminal transferase (Roche), and the 798-nt DNA handle of the transcript was functionalized with biotin on the 5′ end of the PCR primer ...
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bioRxiv - Neuroscience 2021Quote: ... we labeled cell nuclei with DAPI (4’,6-diamidino-2-phenylindole; 1:10.000, Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Genetics 2022Quote: ... purpureum was labeled with DIG using PCR as described in the PCR-DIG Probe Synthesis Kit (Roche Diagnostics). 35S rDNA was labeled with DIG or biotin-16-dUTP (Roche ...
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bioRxiv - Genetics 2022Quote: ... purpureum gDNA probe was labeled with digoxigenin-11-dUTP (DIG) using a DIG Nick Translation Kit (Roche Diagnostics). Hybridization was performed as described in the Instruction Manual of the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Diagnostics) ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were labeled with DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche, Mannheim, Germany), and the operating procedures according to the protocol supplied by the manufacturer of DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche ...
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bioRxiv - Genomics 2019Quote: ... DNA was labeled with Fluorolink Cy3-dUTP (Amersham Pharmacia Biotech; red labeling) using a Nick Translation mix (Roche) or with SpectrumGreen direct-labeled dUTP (Vysis ...
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bioRxiv - Physiology 2020Quote: ... Synthesis of digoxigenin-labeled antisense RNA probes were synthesized using a DIG RNA Labeling Kit (SP6/T7; Roche).
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bioRxiv - Molecular Biology 2020Quote: Digoxigenin (DIG) or fluorescein labeled RNA probes were made using the DIG RNA labeling kit (Roche, Cat. 11175025910) or fluorescein RNA labeling kit (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... Digoxigenin (DIG) and fluorescein labeled (FITC) probes were synthesized by using DIG and FITC RNA labelling kit (Roche). Primer sequences are listed in Table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... and hybridized overnight at 40°C with digoxigenin (DIG)-labeled DNA probes in DIG Easy Hyb solution (Roche). After low stringency washes (washing twice with 2× SSC/0.1% SDS at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: Digoxigenin-labeled RNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics, Basel, Switzerland). In situ hybridizations were performed on 16μm cryosections that were rehydrated in 1x PBS for 5min ...
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bioRxiv - Developmental Biology 2023Quote: ... and the DIG-labeled probes were generated according to the manufacturer’s recommendations (Roche Applied Science, Indianapolis, IN, USA). In situ hybridization was performed on embryos from different stages per detailed published protocols (Wilkinson ...
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bioRxiv - Developmental Biology 2023Quote: Digoxigenin-labeled cRNA probes were prepared via in vitro transcription (DIG RNA labeling kit; Roche Diagnostics, Basel, Switzerland) from the Prokr2 template available from Genepaint (https://gp3.mpg.de/viewer/setInfo/MH1790/12) ...
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bioRxiv - Developmental Biology 2022Quote: ... Both probes were labeled with fluorescein or digoxigenin (DIG) using RNA labeling kit (Roche Applied Science, Mannheim, Germany). The heads of fish at 4 mpf were fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Developmental Biology 2022Quote: ... DIG and fluorescein-labeled probes were detected with anti-DIG or anti-Fluorescein POD-conjugated Fab fragments (Roche) and Cy3 or Fluorescein TSA-plus Reagent (Akoya Biosciences) ...
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bioRxiv - Physiology 2024Quote: Digoxigenin (DIG)-labeled cRNA probes were generated by in vitro transcription using DIG RNA Labeling Mix (Roche Diagnostics), T7 RNA polymerase (Roche Diagnostics) ...
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bioRxiv - Microbiology 2023Quote: ZIK1 sense and antisense RNA probes labeled with digoxigenin were synthesized using the DIG Northern Starter Kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... In vitro transcription of Digoxigenin/Fluorescent-labeled probes was performed using an RNA Labeling Kit (Roche Diagnostics Corporation) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... A digoxigenin labeled probe was generated by PCR following the manufacturer’s instructions (PCR DIG Probe Synthesis Kit,Roche). The 993 bp probe (Cd709 chr1 ...
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bioRxiv - Neuroscience 2024Quote: ... Detection of DIG-labeled probe was performed with a fluorescein-conjugated sheep anti-DIG antibody (1:250, Roche). All buffers were made with RNase-free PBS or water.
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bioRxiv - Microbiology 2024Quote: ... The lcrF transcripts of interest were detected with a DIG-labeled PCR fragment (DIG-PCR nucleotide mix, Roche) amplified with primer pairs for the individually detected according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Mastl immunocomplexes were purified with an anti-GFP antibody (Roche), crosslinked to Dynabeads Protein A (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: PCR amplicons were purified with KAPA Pure magnetic beads (Roche) at a 0.5 × bead-to-sample volume ratio and eluted in nuclease-free water ...