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Citations for Roche :
351 - 400 of 881 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... or 20 μg of fluorescein-labeled probes (see below) for fluorescent detection (Roche, cat. #11685619910). After hybridization ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxygenin-labeled antisense RNA probes were synthesized using a DIG RNA Labeling NTP mix (Roche) and either SP6 or T7 RNA polymerase (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... Probes were synthesized from linearized DNA and labeled during synthesis with digoxigenin-11-UTP (Roche) nucleotides ...
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bioRxiv - Plant Biology 2020Quote: ... transferred to a PVDF membrane and immunoblotted with rat monoclonal anti-HA (High Affinity, clone 3F10, Roche, www.roche.com; 1:2000 dilution) and hybridized with peroxidase conjugated goat anti-rat (Polyclonal ...
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bioRxiv - Biochemistry 2022Quote: ... purified Q23-huntingtin (5 µg) was purified in the presence of complete protease and phosphatase inhibitor cocktails (Roche Applied Science) and incubated with or without PRKACA (20 ng) ...
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bioRxiv - Biochemistry 2022Quote: ... purified by cOmplete His-Tag purification columns (Roche), and concentrated to 1 mg/mL using Amicon Ultra 10,000 NMWL (Merck) ...
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bioRxiv - Cell Biology 2021Quote: ... RNA samples were purified treated with DNase (Roche) and RNeasy mini prep kit (Qiagen) ...
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bioRxiv - Biochemistry 2022Quote: ... Radiolabeled RNAs were purified using QuickSpin columns (Roche) and radioactivity was measured by scintillation counter ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using phenol/chloroform/isoamylalcohol (Roche), precipitated using isopropanol and resuspended in TE ...
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bioRxiv - Cancer Biology 2023Quote: ... purified with KAPA Pure Beads (Roche Molecular Systems), and used as a template for PCR to incorporate Illumina P7 indices ...
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bioRxiv - Developmental Biology 2023Quote: ... then size purified with KAPA Pure Beads (Roche). cDNA input was normalized across samples ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or FITC-labeled antisense probes were synthesized using an RNA Labeling Kit (SP6/T7; Roche). The staining was revealed with NBT/BCIP or INT/BCIP substrate (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: Digoxigenin-labeled antisense Rp-BicC RNA probes were synthesized using the RNA-Dig Labeling kit (Roche). In situ hybridization was carried out in 4% PFA fixed ovaries stored in PBT at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Labeled S and AS probes were generated using the DIG RNA Labeling Kit (SP6/T7) (Roche) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... the DIG-labeled probe was visualized by using alkaline phosphatase-conjugated anti-DIG antibody (Roche Diagnostics) and Fast Red (Roche Diagnostics) ...
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bioRxiv - Neuroscience 2019Quote: ... Dioxygenin (DIG)-labeled probes were detected using peroxidase-conjugated anti-DIG antibodies (Roche Diagnostics, Mannheim, Germany). Peroxidase activity was detected using fluorescein-tyramide conjugate ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 ng of labeled probe was mixed with 10 μg of human Cot-1 DNA (Roche) and 10 μg each of salmon sperm DNA and E ...
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bioRxiv - Plant Biology 2022Quote: ... gene-specific cDNA was amplified and labeled using the DIG RNA Labeling Kit (Roche, Basel, Switzerland). Pretreatment of sections ...
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bioRxiv - Molecular Biology 2022Quote: ... Hybridization with digoxigenin (DIG)-labeled antisense RNA probes was conducted in DIG Easy Hyb (Roche Diagnostics) at 68°C ...
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bioRxiv - Physiology 2022Quote: ... Digoxigenin (DIG) labeled probe was synthesized using the DIG RNA labeling kit (SP6/T7) (Roche, #11175025910). Denatured DIG-labeled probe was hybridized at 55° C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Visualization of the labeled probe was done using anti-DIG-AP (alkaline phosphatase) antibodies (Roche Biochemicals) followed by histochemical enzymatic staining reagents (Roche Biochemicals) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sequences were amplified from cDNA and DIG-labeled RNA probes complementary to the target mRNA (Roche). The primer sequences for synthesizing the probes are available in supplementary table 4 ...
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bioRxiv - Genetics 2019Quote: ... Digoxigenin-labeled RNA probes were prepared using a DIG Northern starter kit (Cat. No. 2039672, Roche) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... DIG-labeled probes were detected using mouse monoclonal anti-DIG primary antibody (Roche; 1:100 dilution) and a Cy3-labeled goat anti-mouse IgG secondary antibody (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The DIG-labeled probe was synthesized using the PCR DIG probe synthesis kit (11636090910, Roche, Switzerland) and the following primers ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After the reaction between DIG-labeled RNA and anti-DIG antibody conjugated horseradish peroxidase (HRP) (Roche), sections were washed 3 times by TNT buffer (100 mM Tris-HCl ...
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bioRxiv - Neuroscience 2019Quote: ... Antisense digoxigenin (DIG)-labeled RNA probes were synthesized using DIG or Fluorescein RNA Labeling Mix (Roche). Human and mouse Cbln2 cDNA ...
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bioRxiv - Developmental Biology 2019Quote: Digoxigenin-labeled cRNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics) from the following templates ...
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bioRxiv - Developmental Biology 2020Quote: Digoxigenin-labeled cRNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics) from the following templates ...
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bioRxiv - Developmental Biology 2022Quote: ... and fluorescein-labeled probes were recognized using an anti-FLUO antibody coupled to peroxidase (11426346910, Roche). Fluorescence signal was produced using the TSA plus kit (NEL753001KT ...
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bioRxiv - Developmental Biology 2023Quote: ... Detection was done with alkaline phosphatase-labeled anti-DIG antibody and NBT/BCIP (Roche diagnostics, USA). Control experiments with sense probes did not give detectable signals (data not shown) ...
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bioRxiv - Neuroscience 2023Quote: ... which was labeled with fluorescein using T7 RNA polymerase and Fluorescein RNA Labeling Mix (Roche Diagnostics), and a DIG-labeled AR (ara ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of fosmid DNA was labeled by nick translation to incorporate biotin-dUTP (Roche 11093070910) or digoxigenin-dUTP (Roche 11093088910 ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of plasmid DNA was labeled by nick translation to incorporate biotin-dUTP (Roche 11093070910), digoxigenin-dUTP (Roche 11093088910 ...
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bioRxiv - Molecular Biology 2023Quote: ... The AAV2 ITR specific probe was labeled using the DIG DNA labeling kit (11175033910, Roche, Switzerland) using the following oligonucleotide ...
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bioRxiv - Plant Biology 2020Quote: ... Western Blotting was performed on 14 day old seedlings to determine protein expression using anti-HA high affinity antibody (Roche, Penzberg, Germany). Primer sequences for RxL21 and RxL21 deletion variants are in Table S9 ...
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bioRxiv - Genetics 2019Quote: ... rat polyclonal anti-Lid (1:500) [13] and mouse monoclonal anti-GFP (Roche #118144600001; 1:200). Secondary antibodies were goat anti-rabbit antibodies (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... The AFS chamber was next functionalized with polyclonal anti-digoxigenin from sheep (Sigma, Roche cat. 11333089001) by injection of 50 μL of a 200 μg/mL anti-Dig solution (20 min incubation) ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies used for immunostaining included anti-HA (1:500, ROHAHA rat polyclonal, clone 3F10, Roche) and anti-GFP (1:2000 ...
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bioRxiv - Plant Biology 2021Quote: ... pH 7.4) for 30 min and subsequently incubated 1 h with anti-HA-peroxidase high-affinity monoclonal rat antibody (3F10; Roche [catalog no. 12013819001]) diluted 1:1000 in 2.5% (w/v ...
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bioRxiv - Genomics 2019Quote: ... and 5 refers to the CRE Number and Affinity library) and with KAPA HiFi HotStart Real-time PCR Master Mix (2X) (Kapa Biosystems, Wilmington, MA) following the recommended cycling protocol at 14 cycles ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labeled RNA probes were synthesized by in-vitro transcription using the DIG RNA labeling kit (Roche). In situ analysis was performed as described previously (Cecchetti et al. ...
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bioRxiv - Neuroscience 2019Quote: ... then used as templates for in vitro transcription with RNA T3 polymerase and DIG-labeled NTPs (Roche) to generate DIG-labeled riboprobes ...
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bioRxiv - Developmental Biology 2019Quote: Antisense riboprobes were transcribed using NEB T7 or T3 RNA polymerase and labeled with digoxygenin (DIG) (Roche). Whole-mount in situ hybridization (WISH ...
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bioRxiv - Cell Biology 2019Quote: ... signals were detected using anti-DIG labeled alkaline phosphatase antibody by chromogen method using NBT/BCIP (Roche). Sections were counter stained with nuclear fast red and mounted after dehydration ...
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bioRxiv - Genetics 2019Quote: Detection of tRNA precursors was performed as described (Otsubo et al., 2018) using Digoxigenin labeled probes (Roche), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 1 h) and labeled with primary antibodies at the following dilutions: rat anti-HA (1:1000; Roche), mouse anti-Myc (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... which was labeled with fluorescein by using Fluorescein RNA Labeling Mix and T7 RNA polymerase (Roche Diagnostics). After blocking with PBS containing 2% normal goat serum for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... PAN specific oligonucleotide probes were labeled with a DIG-Oligonucleotide tailing kit (Sigma Roche 2nd generation 03353583910) and mixed at the 1:1:1 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... Digoxigenin-labeled RNA probes were generated by in vitro transcription using a DIG RNA Labeling Kit (Roche) and purified using Micro Bio-Spin® Chromatography Columns (BioRad) ...