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Citations for Roche :
401 - 450 of 1299 citations for Recombinant Human CD40 protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... The krang digoxigenin-labeled probe was synthesized in vitro using DIG RNA labeling kit (Roche) with T3 (sense ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmid DNA containing EEEb1 was labeled with cyanine-5-dUTP by Nick Translation Mix (Roche), while plasmid DNA harboring each of the other nine DNA families (EEEb2–EEEb10 ...
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bioRxiv - Developmental Biology 2023Quote: ... DIG or fluorescein labeled RNA probes were synthesized by T7 and sp6 RNA polymerases (Roche) from template DNA plasmid digested by NotI or SpeI (New England Biolabs) ...
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bioRxiv - Neuroscience 2022Quote: ... or 20 μg of fluorescein-labeled probes (see below) for fluorescent detection (Roche, cat. #11685619910). After hybridization ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxygenin-labeled antisense RNA probes were synthesized using a DIG RNA Labeling NTP mix (Roche) and either SP6 or T7 RNA polymerase (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... Probes were synthesized from linearized DNA and labeled during synthesis with digoxigenin-11-UTP (Roche) nucleotides ...
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bioRxiv - Molecular Biology 2024Quote: ... the annealed probes were purified with Quick Spin Columns for radio-labeled DNA Purification (Roche). ZNF410 full-length or ZNF410-ZF were overexpressed and harvested from COS-7 cells ...
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bioRxiv - Microbiology 2019Quote: HA-tagged constructs for western blotting were detected using a 1:5000 dilution of anti-HA-peroxidase antibody (Roche clone 3F10, #12013819001). HA-tagged constructs for indirect immunofluorescence were detected using anti-HA (Zymed ...
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bioRxiv - Immunology 2020Quote: ... The remaining tissue was incubated in digestion buffer (RPMI 1640, 10% FCS, 1.25 mg/ml collagenase D (Roche), 0.85 mg/ml collagenase V (Sigma– Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... spleens and were minced and digested in 5 mL of Iscove’s modified Dulbecco’s media (IMDM) + 10% fetal calf serum (FCS) (cIMDM) with 250 μg/mL collagenase B (Roche) and 30 U/mL DNaseI (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... System performance was assessed by an Fc/2 standard obtained from a commercial mAb preparation (Herceptin; Roche Diagnostics).
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bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014 ...
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bioRxiv - Evolutionary Biology 2019Quote: 15-20 µg of total RNA was digested with DNase I (recombinant, RNase-free, Roche Life Science) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: Mice livers were perfused and dissociated into single cells using Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) as previously described8 ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein G beads (Roche) were added for 2 h ...
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bioRxiv - Genetics 2020Quote: ... Protein A beads (Roche) were added for 2h ...
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bioRxiv - Immunology 2020Quote: ... Protein A agarose (Roche) beads were equilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... protein inhibitor cocktail (Roche cOmplete Ultra ...
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bioRxiv - Developmental Biology 2023Quote: ... RT qPCR samples were prepared using the KAPPA SYBR R fast (Roche, KK4611). Primer Sequences:
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or FITC-labeled antisense probes were synthesized using an RNA Labeling Kit (SP6/T7; Roche). The staining was revealed with NBT/BCIP or INT/BCIP substrate (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: Digoxigenin-labeled antisense Rp-BicC RNA probes were synthesized using the RNA-Dig Labeling kit (Roche). In situ hybridization was carried out in 4% PFA fixed ovaries stored in PBT at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Labeled S and AS probes were generated using the DIG RNA Labeling Kit (SP6/T7) (Roche) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... the DIG-labeled probe was visualized by using alkaline phosphatase-conjugated anti-DIG antibody (Roche Diagnostics) and Fast Red (Roche Diagnostics) ...
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bioRxiv - Neuroscience 2019Quote: ... Dioxygenin (DIG)-labeled probes were detected using peroxidase-conjugated anti-DIG antibodies (Roche Diagnostics, Mannheim, Germany). Peroxidase activity was detected using fluorescein-tyramide conjugate ...
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bioRxiv - Plant Biology 2022Quote: ... gene-specific cDNA was amplified and labeled using the DIG RNA Labeling Kit (Roche, Basel, Switzerland). Pretreatment of sections ...
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bioRxiv - Molecular Biology 2022Quote: ... Hybridization with digoxigenin (DIG)-labeled antisense RNA probes was conducted in DIG Easy Hyb (Roche Diagnostics) at 68°C ...
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bioRxiv - Physiology 2022Quote: ... Digoxigenin (DIG) labeled probe was synthesized using the DIG RNA labeling kit (SP6/T7) (Roche, #11175025910). Denatured DIG-labeled probe was hybridized at 55° C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Visualization of the labeled probe was done using anti-DIG-AP (alkaline phosphatase) antibodies (Roche Biochemicals) followed by histochemical enzymatic staining reagents (Roche Biochemicals) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sequences were amplified from cDNA and DIG-labeled RNA probes complementary to the target mRNA (Roche). The primer sequences for synthesizing the probes are available in supplementary table 4 ...
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bioRxiv - Genetics 2019Quote: ... Digoxigenin-labeled RNA probes were prepared using a DIG Northern starter kit (Cat. No. 2039672, Roche) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... DIG-labeled probes were detected using mouse monoclonal anti-DIG primary antibody (Roche; 1:100 dilution) and a Cy3-labeled goat anti-mouse IgG secondary antibody (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The DIG-labeled probe was synthesized using the PCR DIG probe synthesis kit (11636090910, Roche, Switzerland) and the following primers ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After the reaction between DIG-labeled RNA and anti-DIG antibody conjugated horseradish peroxidase (HRP) (Roche), sections were washed 3 times by TNT buffer (100 mM Tris-HCl ...
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bioRxiv - Neuroscience 2019Quote: ... Antisense digoxigenin (DIG)-labeled RNA probes were synthesized using DIG or Fluorescein RNA Labeling Mix (Roche). Human and mouse Cbln2 cDNA ...
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bioRxiv - Developmental Biology 2019Quote: Digoxigenin-labeled cRNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics) from the following templates ...
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bioRxiv - Developmental Biology 2020Quote: Digoxigenin-labeled cRNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics) from the following templates ...
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bioRxiv - Developmental Biology 2022Quote: ... and fluorescein-labeled probes were recognized using an anti-FLUO antibody coupled to peroxidase (11426346910, Roche). Fluorescence signal was produced using the TSA plus kit (NEL753001KT ...
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bioRxiv - Developmental Biology 2023Quote: ... Detection was done with alkaline phosphatase-labeled anti-DIG antibody and NBT/BCIP (Roche diagnostics, USA). Control experiments with sense probes did not give detectable signals (data not shown) ...
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bioRxiv - Neuroscience 2023Quote: ... which was labeled with fluorescein using T7 RNA polymerase and Fluorescein RNA Labeling Mix (Roche Diagnostics), and a DIG-labeled AR (ara ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of fosmid DNA was labeled by nick translation to incorporate biotin-dUTP (Roche 11093070910) or digoxigenin-dUTP (Roche 11093088910 ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of plasmid DNA was labeled by nick translation to incorporate biotin-dUTP (Roche 11093070910), digoxigenin-dUTP (Roche 11093088910 ...
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bioRxiv - Molecular Biology 2023Quote: ... The AAV2 ITR specific probe was labeled using the DIG DNA labeling kit (11175033910, Roche, Switzerland) using the following oligonucleotide ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.01 mg/mL human insulin (Roche, Indianapolis, IN, USA), 100 IU penicillin (Mediatech) ...
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bioRxiv - Cancer Biology 2023Quote: ... human PR rabbit monoclonal antibody (Roche Diagnostics, 790-4296), and human Ki67 rabbit monoclonal antibody (Roche Diagnostics ...
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bioRxiv - Immunology 2023Quote: ... human interleukin-2 (IL-2) (10 IU/ml, Roche), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were first pre-incubated with 2.4G2 antibody (kindly provided by Louis Boon) for blocking Fc receptors and next stained for surface markers in PBS supplemented with 0.5% BSA (Roche) and 2 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Genetics 2021Quote: Total proteins were extracted using protein extraction reagents (Roche Diagnostics Ltd., Shanghai, China) from the myocardial tissue of rats ...
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bioRxiv - Developmental Biology 2022Quote: ... Positive control embryos were incubated in polymerase chain reaction buffer containing 3 U/ml DNase I recombinant (Roche) for 1 h at 37°C before adding the TUNEL reaction mix ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mg of nuclear extract of KDM3A and KDM3B endogenously tagged ESCs (prepared as above) was incubated with 10 µg of HA antibody (Roche, clone 12ca5, catalog#11583816001) for 4 hours rotating at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labeled RNA probes were synthesized by in-vitro transcription using the DIG RNA labeling kit (Roche). In situ analysis was performed as described previously (Cecchetti et al. ...