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Citations for Roche :
451 - 500 of 1299 citations for Recombinant Human CD40 protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... then used as templates for in vitro transcription with RNA T3 polymerase and DIG-labeled NTPs (Roche) to generate DIG-labeled riboprobes ...
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bioRxiv - Developmental Biology 2019Quote: Antisense riboprobes were transcribed using NEB T7 or T3 RNA polymerase and labeled with digoxygenin (DIG) (Roche). Whole-mount in situ hybridization (WISH ...
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bioRxiv - Cell Biology 2019Quote: ... signals were detected using anti-DIG labeled alkaline phosphatase antibody by chromogen method using NBT/BCIP (Roche). Sections were counter stained with nuclear fast red and mounted after dehydration ...
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bioRxiv - Genetics 2019Quote: Detection of tRNA precursors was performed as described (Otsubo et al., 2018) using Digoxigenin labeled probes (Roche), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 1 h) and labeled with primary antibodies at the following dilutions: rat anti-HA (1:1000; Roche), mouse anti-Myc (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... which was labeled with fluorescein by using Fluorescein RNA Labeling Mix and T7 RNA polymerase (Roche Diagnostics). After blocking with PBS containing 2% normal goat serum for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... PAN specific oligonucleotide probes were labeled with a DIG-Oligonucleotide tailing kit (Sigma Roche 2nd generation 03353583910) and mixed at the 1:1:1 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... Digoxigenin-labeled RNA probes were generated by in vitro transcription using a DIG RNA Labeling Kit (Roche) and purified using Micro Bio-Spin® Chromatography Columns (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... The DIG-labeled probes were hybridized to the membrane in DIG Easy Hyb hybridization buffer (Roche #11603558001) at 49°C for overnight ...
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bioRxiv - Neuroscience 2021Quote: ... The probes were then labeled using a digoxigenin (DIG) RNA labeling Kit (Roche Diagnostics Corp., Indianapolis, IN). After pre-hybridization ...
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bioRxiv - Physiology 2022Quote: ... Detection of DIG-labeled probes was performed by incubating with anti-DIG antibody (1:2000, Roche #11093274910) in 0.1% PBST overnight at 4° C ...
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bioRxiv - Developmental Biology 2020Quote: Riboprobes were made using T3 and T7 polymerases and a digoxigenin-labeled ribonucleotide mix (Roche, Cat.# 11277073910) using PCR templates ...
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bioRxiv - Molecular Biology 2019Quote: ... The Bar amplified fragment (Table 1) labeled by DIG high primer DNA labeling (Roche, Cat. No. 11585614910) and purified using a high pure PCR product purification kit (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... and digoxigenin-labeled RNA probes were transcribed using a digoxigenin-UTP in vitro transcription labeling mix (Roche) and either T7 or T3 RNA polymerase (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... and used as template to generate DIG-labeled in situ probes with SP6/T7 polymerases (Roche, # 10999644001). Whole-mount in situ hybridization was done according to Lécuyer et al ...
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bioRxiv - Molecular Biology 2022Quote: ... probes were digoxigenin-(DIG) labeled by using the DIG RNA Labeling Kit (Roche Co. Ltd, Basel, Switzerland). The female flower buds collected at 1 DAP were infiltrated with the FAA fix solution (90 ml of 50% (v/v ...
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bioRxiv - Cell Biology 2022Quote: Digoxin-labeled RNA probes were in vitro transcribed as per manufacturers’ instructions (DIG RNA labeling Mix, Roche; T7 polymerase ...
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bioRxiv - Developmental Biology 2022Quote: ... opilio used sense (control) and antisense RNA probes labeled with DIG RNA labeling mix (Roche, Basel, Switzerland) and followed published protocols (Sharma et al ...
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bioRxiv - Developmental Biology 2023Quote: ... A DIG-labeled antisense RNA probe targeting lefty1 was synthesized using a DIG Probe Synthesis Kit (Roche). NBT/BCIP/Alkaline phosphatase-stained embryos were dehydrated in methanol before clearing and imaging in 2:1 benzyl benzoate:benzyl alcohol (BBBA ...
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bioRxiv - Molecular Biology 2023Quote: ... DIG-labeled TER and alpha-tubulin probes were generated using the PCR DIG Probe Synthesis Kit (Roche) and used in the hybridization step (S1 Table ...
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bioRxiv - Cell Biology 2023Quote: ... RNA probes were transcribed and labeled with either FITC (fluorescein isothiocyanate) or DIG (digoxigenin) NTPs (Roche Cat#).
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bioRxiv - Genomics 2024Quote: ... The probe was labeled with alpha-32P CTP using the High Prime DNA labeling kit (Roche, #11585584001). The labelled probe was purified on a G50 sephadex column (Cytiva ...
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bioRxiv - Genetics 2023Quote: ... Bound protein/protein complexes were washed 3 times ((1x TBS, 0.5% Nonident P40 (Roche), 1% phosphatase inhibitor cocktail 2 and 3 (Sigma)) ...
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bioRxiv - Immunology 2022Quote: ... Tissue pieces then were transferred to a 50-mL conical tube containing 5 ml of digestion medium #2 (RPMI without phenol red containing 5% of FCS, 25 µg/ml of Liberase (Roche), and 200 µg/ml of DNase I (Sigma)) ...
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bioRxiv - Immunology 2019Quote: A single-cell suspension of lung cells was obtained by mincing whole mouse lungs in RPMI and then digesting each lung in 5 mL of RPMI + 10% FCS with 1 mg/mL Collagenase A (Roche) and 2000 U/mL of DNAse I for 45 minutes in a shaking incubator at 37°C ...
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bioRxiv - Immunology 2020Quote: ... The mucosa was stripped and dissociated in GentleMACS tubes in digestion medium (DM; complete medium (RPMI 1640 with PGA/L-glutamine/10% FCS) with 1 mg/mL Collagenase D (Roche), 1 mg/mL soybean Trypsin inhibitor (Sigma) ...
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bioRxiv - Genetics 2024Quote: ... In order to link the sequencing adaptors and double indexed libraries the second PCR step were performed using TG Nextera® XT Index Kit (FC-131-200(1,2,3) and the Kappa Hifi Hot Start (Roche KK2602) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... All lung lobes were cut into small pieces and digested in R5 media (RPMI 1640 supplemented with FCS (5 %) containing DNase I grade II (30 µg/ml) and Liberase TM (50 µg/ml, Roche) for 45 min at 37 °C with a magnetic stirrer.
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bioRxiv - Developmental Biology 2019Quote: ... and protein G agarose (Roche). Immunoprecipitates were analyzed on SDS-PAGE gel bands ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein A Agarose (Roche 11134515001), blocked with 10μg BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Protein-A agarose beads (Roche) washed three times with lysis buffer were mixed with IPed lysates and incubated at 4 °C for two hours or overnight with constant rotation ...
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Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2bioRxiv - Molecular Biology 2019Quote: ... and protein-G-agarose (Roche). Oligonucleotide complexes were labelled with alpha-32P dCTP using terminal deoxynucleotidyl transferase (Fermentas ...
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bioRxiv - Immunology 2019Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein-G agarose beads (Roche) were then added ...
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bioRxiv - Immunology 2020Quote: ... protein inhibitor cocktail (PIC) (Roche). Cross-linked cell pellets were stored at −80°C ...
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bioRxiv - Microbiology 2023Quote: ... protein G agarose beads (Roche) were added to the cell lysate and incubated for 3 h at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein A-Agarose (Roche, 11134515001) and Protein G-Agarose (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein G Sepharose beads (Roche) were pre-incubated with 1 µg of indicated primary antibody for 2 h at 4°C ...
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bioRxiv - Immunology 2019Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The A20 cells were grown in RPMI-1640 (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and 100 U/mL human interleukin 2 (IL-2) (Roche) (Munoz et al. ...
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bioRxiv - Biophysics 2019Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche).
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bioRxiv - Cell Biology 2021Quote: ... human NEMO (5 µg) and ATP (2 mM) (Roche, 10519979000) were incubated at 37°C for the indicated time in a buffer containing 150 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... coated with 5 µg/ml human plasma fibronectin (Roche, 11080938001) in growth medium overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... and human Ki67 rabbit monoclonal antibody (Roche Diagnostics, 790-4286). Images were captured with VENTANA iScan HT (Roche Diagnostics ...
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bioRxiv - Immunology 2023Quote: ... and 30 IU/mL human interleukin-2 (hIL-2) (Roche). To ectopically express Lifeact-eGFP or Lamp1-eGFP in CTLs ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.01 mg/mL of human insulin (Roche, Indianapolis, IN, USA), 10 mM HEPES (Corning ...
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bioRxiv - Cell Biology 2019Quote: ... 500mM NaCl, 1mM TCEP-HCl (ThermoScientific, 20491) pH 7.4 which was supplemented with recombinant DNase I (1000U) (Roche,04536282001), cOmplete protease EDTA-free inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... 12-20 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) with 2.3 U of Liberase Blendzyme 3 recombinant collagenase (Roche) were cannulated into the vena cava to isolate hepatocytes ...