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Citations for Roche :
401 - 450 of 538 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The cells were further incubated for 48 h and subsequently subjected to Gentamycin (G418) selection (1 μg/ml, Roche, G418-RO). After 4 days cells were sorted on a fluorescence-activated cell sorter (FACS ...
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bioRxiv - Cell Biology 2023Quote: Library preparation was performed with 500 ng of total RNA where the ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems), fragmented in reverse transcriptase buffer and heated to 94°C for 8 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... CDMs were washed with PBS and treated for 1 h at 37°C with a buffer of 50 µg/ml DNaseI (Roche, #11284932001), 5 mM MgCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... samples were covered with the staining mixture at 37°C for 1 h following instructions of the In Situ Cell Death Detection Kit (Roche, 11684795910). After the TUNEL reaction ...
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bioRxiv - Cell Biology 2024Quote: HEK293T cells were seeded at 30 000/45 000 cells/cm2 and transfected 24 h after with plasmids (pcDNA3.1-GFP, MPV17-HA or MPV17) preincubated with XTremeGENE HP Transfection Reagent (Roche, Basel, Switzerland) for 30 min at room temperature in Opti-MEM I serum-free medium (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was blocked in 5% milk in PBS with 0.1% Tween 20 (PBS-T) for 1 h at room temperature (RT) before being probed with either rat anti-HA-tag antibody (Roche, 11867423001) diluted 1:1000 in 5% milk in PBST ...
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bioRxiv - Cell Biology 2024Quote: ... Eluates were transferred to fresh tubes and incubated for 2 h at 55°C with 0.3 µg/µl proteinase K (Roche, cat# 3115852001). For the chromatin input sample ...
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bioRxiv - Cancer Biology 2020Quote: ... and pancreatic tumor bearing mice by mechanical separation of the tumor followed by digestion with an enzymatic cocktail consisting of 1mg/ml collagenase A and collagenase D and 0.4mg/ml DNase I (all from Roche) in PBS at 37°C for 2-3h with rotation at 600rpm ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected and digested for 30min at 37°C using RPMI media (2% FBS+ Pen/Strep, glutamine, 10mM HEPES) containing 0.5mg/mL Collagenase D (Roche). The digested fragments were passed through a 70μm cell strainer (Greiner bio-one ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) 2-mercaptoethanol and 60 × 10−3 М n-Octyl-β-D-Glucopyranoside) containing protease inhibitor (Roche). Total protein was collected and incubated with anti-p75NTR (Alomone ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg poly[d(I-C)] and 0.1 μg poly-Lysine (based on DIG Gel Shift Kit, 2nd Generation, Roche) for 15 min at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The imaginal discs were removed and suspended whole in 350μl of sucrose solution (250mM D-sucrose, 10mM Tris-HCl, 1mM. MgCl2, 1x protease inhibitors (Roche)) inside labelled 2ml dounce homogenisers (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... C-reactive protein and D-dimer levels were measured using Cobas Integra 400 plus analyzer (Roche Diagnostics Nederland B.V.).
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bioRxiv - Immunology 2021Quote: ... ankle joints were teased apart and shaken at 110 rpm at 37°C for 25 minutes with 2.68mg/mL collagenase D (Roche) in RPMI 1640 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: Tissue leukocytes were extracted from surgically dissociated lung tissue by enzymatic digestion with 2ml enzyme mix (RPMI with 0.625 mg/ml collagenase D (Roche), 0.85 mg/ml collagenase V (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... A single-cell solution was obtained by tissue digestion using 1mgml−1 collagenase D and 0.1mgml−1 DNase I (Roche) in HBSS (Lonza ...
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bioRxiv - Immunology 2021Quote: ... For experiments in which islets were analyzed using flow cytometry single cell suspensions were generated by digestion with 0.4 Wunsch Units/ml Collagenase D (Roche) and 250 µg/ml DNAse I (Roche ...
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bioRxiv - Immunology 2022Quote: ... Collected tissue was mechanically dissociated by chopping followed by enzymatic digestion for 45 minutes at 37°C in DMEM containing collagenase D (1.5 mg/ml, Roche) and DNase I (60 U/ml ...
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bioRxiv - Cell Biology 2021Quote: ... at 37°C in a 0.4 μm filtered solution of 0.5 mg/mL collagenase D (Roche, Indianapolis, IN, USA), 1 Unit/mL Dispase (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... brain and dura mater were chopped and subject to enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 2.5 mg/mL collagenase D (Roche) and 0.1 mg/mL DNase I (Roche ...
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bioRxiv - Immunology 2021Quote: ... Livers and liver tumours were excised and then minced with scissors before incubation in RPMI/10% (v/v) FBS/2mM EDTA/1.2mg/mL Collagenase D (Roche)/1mg/mL DNase I (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were dissociated in 1-2 ml of digestion mix (D-PBS, 250ug/ml Liberase TL (Roche; cat: 5401020001), 0.1 mg/ml DNaseI (Sigma ...
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bioRxiv - Immunology 2021Quote: L1/L2 larvae were collected via pipette in 200-300 µl of PBS then added directly to lysing matrix D (1.4-mm ceramic spheres, MagNA Lyser Green Bead tubes, Roche) along with 1 ml of TRIzol LS ...
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bioRxiv - Immunology 2020Quote: ... The organs were chopped mechanically with scalpels and transferred into gentleMACS C tubes containing DMEM supplemented with 1μg/mL Collagenase D (Roche) and 25mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Right lung lobes were chopped finely and digested in pre-warmed RPMI1640 with ‘collagenase cocktail’ (0.625mg ml−1 collagenase D (Roche), 0.425mg ml−1 collagenase V (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and lung were further digested in RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche) on a shaker at 220rpm for 45 minutes at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... cells were released by incubating gels in a cell culture incubator in a protease solution of 2.5 mg/mL collagenase D (Roche) in PBS until gels were fully degraded ...
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bioRxiv - Immunology 2023Quote: ... Brain and spinal cord were cut into pieces and digested for 30 min at 37 °C with collagenase D (3.75 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... enzymatic digestion of tissue pieces was done with collagenase D (1 mg/ml) and DNAseI (0.1 mg/ml) (both from Roche) in HBSS/10%FBS for 45 min (37°C ...
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bioRxiv - Immunology 2023Quote: ... Popliteal lymph nodes were harvested from immunized mice 1 day later and digested with collagenase D (2.5 mg/mL, Roche) in RPMI-1640 medium supplemented with 10% FBS for 30 min at 37°C ...
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bioRxiv - Immunology 2024Quote: ... kidneys were cut and digest for 30 min at 37°C with 0.5mg/ml collagenase D (Roche, Mannheim, Germany) and 0.05 mg/ml DNase I (Roche ...
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bioRxiv - Immunology 2024Quote: Lung tissue was prepared by coarsely chopping the tissue followed by incubation in a 0.5 mg/mL solution of collagenase D (Roche) and 500 Units of DNase (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Lungs were processed with a gentle MACS dissociator (Miltenyi Biotech) according to manufacturer’s protocol and digested with Collagenase D (11mg/ml; Roche) and DNase I (301µg/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% Normal Goat Serum) for 3 h at room temperature and then incubated overnight with alkaline phosphatase-conjugated anti-DIG Fab fragments (Roche, 1: 2,000) at 4°C on a shaking platform ...
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bioRxiv - Molecular Biology 2021Quote: ... were transiently transfected for 24 h with 9,36 μg of hNAA40-flag expression vectors and using 35 μl Fugene HD (Roche, cat. n°04709705001) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Transfection with full-length nV5-WT or nV5-P112L (0.8 μg DNA/well) was performed 24 h post-seeding using X-tremeGENE™ 9 DNA Transfection Reagent (Roche, Basel, Swityerland). 24 h post-transfection ...
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bioRxiv - Microbiology 2021Quote: The body weight of all mice was recorded weekly and blood glucose (BG) levels were measured every 5 weeks after fasting for 12 h using a glucose analyzer (Accu-Chek; Roche, Rotkreuz Switzerland) over the tail vein ...
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bioRxiv - Cancer Biology 2021Quote: ... control and Capecitabine treated tumors were digested for 2 h at 37 °C with a cocktail of Collagenase I (Roche, Ref: 11088793001) and Hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... the LDH release in cell culture supernatant from infected cells was measured at 24 h and 96 hpi by using the colorimetric kit (Roche, Mannheim, Germany) and following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... sections were blocked with 10% HI-serum for 2 h and incubated overnight at 4°C with alkaline phosphatase-labeled sheep anti-dig antibody (11093274910, Roche; 1:2500). After washing ...
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bioRxiv - Plant Biology 2020Quote: ... In situ nick-end labeling of nuclear DNA fragmentation was performed for 1 h in the dark at 37°C using the In-Situ cell death detection kit (Roche Applied Science) according to the manufacturer’s manual ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked for 1 h with 3% (w/v) non-fat milk powder in PBS/0.1% (v/v) Tween (PBST) (CALR, 10% Roche WBR in PBST) and incubated with primary antibody (Supplementary Table III ...
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bioRxiv - Microbiology 2020Quote: ... Samples were incubated alone or with ipICP4/pICP4 or BSA for 1 h at 4°C and subsequently fluorescence intensity was measured in a LightCycler II (Roche, Milan, Italy) by observing 6-carboxyfluorescein (6-FAM ...
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bioRxiv - Immunology 2022Quote: ... cells were collected 24 h after incubation with 40 ng/ml IL-10 following the protocol of the High Pure RNA Isolation Kit (Roche, Basel, Switzerland).
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bioRxiv - Microbiology 2023Quote: ... HEK293T/17 cells were seeded onto a 6-well plate and then transfected 24 h later using X-tremeGENE HP (Roche, Basel, Switzerland). After transfection (24 h ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated at 37 °C in the dark for 1 h with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) solution from the In Situ Cell Death Detection Kit (Roche, Mannheim, Germany). The embryos were washed three times with PBS-PVP ...
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bioRxiv - Physiology 2023Quote: ... autofluorescence was quenched by treating paraffin-embedded sections with PBS/BSA (5%) for 2 h before performing TUNEL staining (Roche, Basel, Switzerland) according to the manufacturer’s protocol and using Proteinase K treatment ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in MBST + 10% HISS + 2% BMB for 2 h at room temperature and incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910) diluted in blocking buffer until signal development ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were stained with secondary antibodies (1:300) for 2 h at room temperature and nuclei stained with DAPI (1:1000, Roche, Munich, Germany). Cells were mounted with ProLong Diamond Antifade Mountant (Thermo P36970 ...