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Citations for Roche :
501 - 538 of 538 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Kidneys were finely minced and digested for 40 min at 37°C with 0.4 mg/ml collagenase D (Roche, Mannheim, Germany) and 0.01 mg/ml DNase I (Roche) ...
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bioRxiv - Immunology 2022Quote: Skin biopsies were collected and minced with sterile scissors before digestion in 1 mL of DMEM with 1 mg/mL collagenase D (Roche Diagnostics) for 3 hours at 37℃ ...
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bioRxiv - Immunology 2022Quote: ... We transferred the minced tissue to a tube containing 5 ml of digestion buffer containing collagenase D (2mg/ml, Roche #11088858001) and DNase (0.125 mg/ml ...
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bioRxiv - Immunology 2022Quote: ... these tissues were dissected and incubated for 1h at 37ºC in the enzymatic cocktail containing Colleagenase D (Roche, 1 mg/ml) and DNAse I (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... the pellet was resuspended in 200 µl cold buffer D (50 mM Tris/HCl (pH=8.0), 10 mM EDTA, 1% SDS, 1xPIC (cOmplete™, Protease Inhibitor Cocktail (Roche; 04693116001)) per 15 cm plate ...
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bioRxiv - Pathology 2023Quote: ... and kidneys were minced into small pieces and placed into 5mL digestion buffer (15mg/mL Collagenase D (Roche Applied Science, 11088858001), 10mg/mL Collagenase Type 4 (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... the pellet was resuspended in 200 µL of cold buffer D (50 mM Tris/HCl, pH = 8.0, 10 mM EDTA, 1% SDS 1xPIC (cOmplete™, Protease Inhibitor Cocktail; Roche; 04693116001)) per 15 cm plate ...
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bioRxiv - Immunology 2023Quote: ... and digested at 37°C for an hour with mechanical disruption with a stir bar and enzymatic digestion with collagenase D (Roche 11088875103) and DNase I (Sigma D4527) ...
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bioRxiv - Immunology 2023Quote: ... in HBSS for 20 min and then incubated for 20 min with and equal volume of DMEM with Collagenase D (Roche, #11088858001) and DNase I (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... Cultures were grown until an A600 of 0.6-0.8 was reached and protein over-expression was induced by incubating with 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG, Roche) for 16 hours at 18°C.
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bioRxiv - Cancer Biology 2024Quote: ... The tissues were then cut into small pieces using sharp dissection scissors and incubated in 10 mL dissociation solution (0.2 mg/mL Collagenase D (Roche, Cat#11088866001); 0.05 mg/mL DNase I (Roche ...
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bioRxiv - Microbiology 2021Quote: DNA protein interaction was studied with purified H-NS and DIG labeled PCR amplified PctxAB fragments by using the DIG gel shift kit (2nd Gen, Roche Aplied Science, Mannheim, Germany) as previously described (10) ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were transfected 24 h prior to the experiment with cDNA of interest with Fugene6 following the protocol of the manufacturer (Roche Diagnostics, Burgess Hill, UK). MCF10A cells [CRL-10317 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated for at least 1 h with a 1:1000 dilution of a high affinity anti-HA antibody from rat IgG1 (#11867423001, Roche Applied Science, Penzberg, Germany). Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795 ...
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bioRxiv - Microbiology 2022Quote: ... 0.85 mg/ml; Collagenase D, Roche, 11088882001, 1.25 mg/ml; dispase, Gibco, 17105-041, 1 mg/ml; DNase, Roche, 101104159001, 30µg/ml). Tissues were then incubated at 37 °C with shaking for at least 15 min and manually shaken every 5 minutes until complete digestion ...
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bioRxiv - Immunology 2022Quote: ... The activation of B3Z cell line was measured detecting β-galactosidase activity by optical density (absorbance at 595–655 nm) using chlorophenol red-β-D-galactopyranoside (CPRG; Roche) as substrate for the reaction.
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bioRxiv - Cell Biology 2022Quote: ... and processed in digestion buffer (1 mg/ml of Collagenase D and 80 U/ml DNase I, both from Roche, in DMEM) and a GentleMACS dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... These tissues were cut to ∼2mm pieces and incubated for 1h at 37ºC in the enzymatic cocktail containing Colleagenase D (Roche, 1 mg/ml) and DNAse I (Roche ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The left lobe minced into small (~1-2 mm) sections and digested using 0.5 mg/mL collagenase D (Roche Diagnostics, Mannheim, Germany) and 50 U/mL DNase I (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: Resected tumors were cut into small pieces using spring scissors and digested in 0.5 mg/mL collagenase D (Roche: cat#: COLLD-RO) and 0.25 mg/mL DNase I at 37°C for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... and PLN from non-diabetic 6w– 8w female mice were minced and enzymatically dissociated with 1 mg/mL collagenase D (Roche Applied Science) and 25 U/mL DNase I in DMEM for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Consecutive sections were stained with hematoxylin and eosin (H&E) or subjected to immunohistochemistry using automated immunostaining platforms (Ventana Discovery XT, Roche or Autostainer Plus Link 48). Antigen retrieval was first performed with high or low pH buffer depending on the primary antibody (CC1m ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Immunology 2021Quote: ... Lungs were mechanically dissociated by using a scalpel and transferred to collagenase solution consisting of DMEM supplemented with collagenase D (Roche, 1 mg/ml) and DNase1 (ThermoFisher ...
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bioRxiv - Physiology 2020Quote: ... finely cut with scissors and incubated in digestion medium (PBS with 2% BSA, 1M CaCl2, 94U/ml dispase II and 100mg/ml collagenase D, Roche Life Science, France) at 37°C for 30-40min ...
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bioRxiv - Bioengineering 2022Quote: ... Fetal livers were cut in small fragments and then treated for 45 min at 37°C with collagenase D (Roche; 100 ng/ml), and a cell suspension was prepared ...
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bioRxiv - Immunology 2024Quote: ... Brains were homogenized using a glass Potter and digested for 45 min at room temperature (RT) in Hanks’ balanced salt solution (HBSS) medium with collagenase D (1 mg/ml, Roche Diagnostics cat # 11088882001) and deoxyribonuclease (DNase ...
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bioRxiv - Biochemistry 2023Quote: ... and collected by centrifugation at 7,500 RCF for 15 minutes and then suspended in Lysis Buffer D (20 mM HEPES pH 8, 200 mM NaCl, 5 mM imidazole, 0.5 mM Imidazole, 1x Roche EDTA free protease inhibitor). Cells were lysed using an emulsifier (AvestinEmulsiflexC3 ...
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bioRxiv - Cancer Biology 2024Quote: ... To maintain the carryover ≤ 20% with intra-day and inter-day (2 d) CV%≤ 20% acceptable for bioanalytical assays ((Clouser-Roche et al., 2008), it was necessary to perform intermediate a post-run wash injection following each standard and sample injection ...
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bioRxiv - Immunology 2024Quote: ... lungs were minced with scissors and then enzymatically digested for 45 min at 37°C in RPMI-1640 medium supplemented with 1 mg/ml Collagenase D (Roche-Diagnostics, Indianapolis, IN), 1 mg/ml hyaluronidase ...
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bioRxiv - Systems Biology 2024Quote: Tissue samples were homogenized using the FastPrep-24 Instrument using lysing matrix D in lysis buffer (8M urea, 200 mM EPPS, Roche protease inhibitor tablets) and cells sonicated ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 19 hindguts were minced with a blade and incubated for 30 min at 37°C in a solution of Collagenase D (Roche, 1 mg/mL). After filtering in a 40 μM strainer and washing with BGJb ...
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bioRxiv - Microbiology 2024Quote: In-situ detection of DNA fragments following treatment of cells with MLT 5 μM and 10 μM for 24 h was measured by TUNEL using a Cell Death Detection kit (In-Situ Cell Death Detection Kit, POD; Roche; Cat. No. 11 684 817 910). 1000 μL of cells were centrifuged (3000 rpm for 5 min) ...
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bioRxiv - Plant Biology 2020Quote: ... yeast strains were grown for 2 d on non-selective medium and the β-galactosidase enzymatic activity was determined in an assay using red-β-D-galactopyranoside (CPRG, Roche Applied Science) as substrate (Yeast Protocols Handbook ...
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bioRxiv - Genomics 2020Quote: ... the tissues were cut into 1-2 mm2 pieces and put in 1mg/ml collagenase and dispase (C/D) solution (Roche Diagnostics GmbH Roche Applied Science, Mannheim, Germany) at 37°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... and S-D-Bact-0785-a-A-21 (GACTACHVGGGTATCTAATCC) (Klindworth et al. 2013) together with KAPA HIFI Hot Start Ready Mix (Kapa Biosystems, Cat. No. 07958935001) containing the polymerase with proofreading activity ...
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bioRxiv - Immunology 2024Quote: ... and digested for 45min in digestion solution composed of Hanks’ balanced salt solution supplemented with collagenase D (1 mg/ml, Roche Diagnostics cat # 54 11088882001) and deoxyribonuclease (DNase ...
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bioRxiv - Neuroscience 2021Quote: ... were collected from CX3CR1GFP/+ mice and incubated isolated in 1 ml of RPMI medium containing 1mg/ml of collagenase D (Roche, cat. no. 1-088-874) for 30 minutes at 37°C ...